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Duf_1100-R

Relationship

Family: Duf_1100-R

Block: X

Parent Family: UPF0255

Comment

DUF1100 UPF0255. The family was split in two. Duf_1100-S with active site (S217 in (GXSXG) D300 H329) as in artni-Q93NG6, and Duf_1100-R with active site (R53, D203, R 272 (in GXRXG)) as in FrsA. The fermentation/respiration switch protein FrsA (product of the yafA gene in E. Coli (ecoli-yafa)(vibvy-y856) binds the dephosphorylated form of glucose-specific enzyme IIAGl the interaction increases the glucose fermentation under oxygen-limited conditions. FrsA is a pyruvate decarboxylase (Lee et al.)(In Scop 2,6-dihydropseudooxynicotine hydrolase). Thus there are two functions and two different active sites in this family (nucleophile elbow: GXSXG in hydrolase, GXRXG in decarboxylase). However (Kellett et al.) showed computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase. Other members of this family are still uncharacterised

Database

Interpro : IPR010520 Esterase FrsA-like Protein of unknown function DUF1100 UPF0255, hydrolase-like , IPR043423 Esterase FrsA

PIRSF : PIRSF004493 YafA protein

Pdoc : No Pdoc

Pfam : PF06500 FrsA-like DUF1100

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
3MVE Crystal structure of a novel pyruvate decarboxylase vibvy-y856
3OUR Crystal structure of complex between EIIA and a novel pyruvate decarboxylase vibvy-y856
4I4C Crystal structure of the protein frsA complexed with unknown ligand vibvy-y856

Gene_locus Name Species
9entr-b6xas1 Providencia alcalifaciens DSM 30120 Putative uncharacterized protein Providencia alcalifaciens DSM 30120
9entr-d1p5v4 Providencia rustigianii DSM 4541 Esterase FrsA Providencia rustigianii DSM 4541
9entr-d2u045 Arsenophonus nasoniae (son-killer infecting Nasonia vitripennis) Esterase Arsenophonus nasoniae
9entr-d4bdn7 Citrobacter youngae ATCC 29220, Citrobacter sp. 30_2 , UPF0255 protein CIT292_08605 Citrobacter youngae ATCC 29220
9entr-e5yif6 Enterobacteriaceae bacterium 9_2_54FAA UPF0255 protein HMPREF0864_02316 Enterobacteriaceae bacterium 9_2_54FAA
9gamm-a5kz13 Vibrionales bacterium, Vibrio sp., Vibrio splendidus (Vibrio splendidus) Fermentation\/respiration switch protein Vibrionales bacterium SWAT-3
9gamm-e8lht3 Succinatimonas hippei YIT 12066 Putative uncharacterized protein Succinatimonas hippei YIT 12066
9gamm-q1zfr4 Psychromonas sp. CNPT3 hypothetical protein Psychromonas sp. CNPT3
9gamm-q2c662 Photobacterium sp. SKA34 hypothetical protein Photobacterium sp. SKA34
9vibr-a6cwr8 Vibrio shilonii AK1 Fermentation\/respiration switch protein Vibrio shilonii AK1
9vibr-a8tap9 Vibrio sp. AND4 Fermentation\/respiration switch protein Vibrio sp. AND4
9vibr-c9nnl3 Vibrio coralliilyticus ATCC BAA-450 UPF0255 protein VIC_000672 Vibrio coralliilyticus ATCC BAA-450
9vibr-e3blh9 Vibrio caribbenthicus ATCC BAA-2122 Fermentation\/respiration switch protein Vibrio caribbenthicus ATCC BAA-2122
9vibr-e8m7m8 Vibrio sinaloensis DSM 21326, Vibrio brasiliensis LMG 20546, Fermentation\/respiration switch protein Vibrio sinaloensis DSM 21326
phoas-q1zpm9 Photobacterium angustum (strain S14 \/ CCUG 15956) (Vibrio sp. (strain S14 \/ CCUG 15956) hypothetical protein Photobacterium angustum S14
aerhh-a0knr1 Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 \/ NCIB 9240) Esterase YafA Aeromonas hydrophila subsp. hydrophila
aers4-a4sjf3 Aeromonas salmonicida (strain A449) Putative uncharacterized protein Aeromonas salmonicida
alisl-b6eif0 Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) Putative esterase Aliivibrio salmonicida
chrvo-q7p151 Chromobacterium violaceum hypothetical protein Chromobacterium violaceum
citk8-y2957 Citrobacter koseri (strain ATCC BAA-895 \/ CDC 4225-83 \/ SGSC4696) UPF0255 protein CKO_02957 Citrobacter koseri
citri-d2tjy8 Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280) UPF0255 protein ROD_03341 Citrobacter rodentium
cros8-a7men1 Cronobacter sakazakii (Enterobacter sakazakii) Cronobacter turicensis UPF0255 protein ESA_03114 Ctu_08510 Cronobacter sakazakii
dicd3-e0scu7 Dickeya dadantii (Erwinia chrysanthemi)\; Dickeya solani UPF0255 protein Dda3937_00932 Dickeya dadantii
dicd5-d2bv10 Dickeya dadantii (strain Ech586) UPF0255 protein Dd586_3245 Dickeya dadantii
dicdc-c6cb38 Dickeya dadantii (strain Ech703) UPF0255 protein Dd703_0931 Dickeya dadantii
dicze-c6cmk3 Dickeya zeae (strain Ech1591) UPF0255 protein Dd1591_0898 Dickeya zeae
ecoli-yafa Escherichia coli, Escherichia fergusonii, Escherichia albertii, (EC 3.1.-.-) esterase yafa Fermentation-respiration switch protein frsA Escherichia coli
edwtf-e0t1p5 Edwardsiella tarda, Edwardsiella ictaluri, UPF0255 protein ETAF_0736 Edwardsiella tarda
ent38-y765 Enterobacter sp., Enterobacter cancerogenus, Enterobacter cloacae UPF0255 protein Ent638_0765 Enterobacter sp.
entls-e3g7m4 Enterobacter cloacae (strain SCF1) UPF0255 protein Entcl_3479 Enterobacter cloacae
erwac-d4hxs4 Erwinia amylovora (strains CFBP1430\; ATCC BAA-2158\; ATCC 49946 \/ CCPPB 0273 \/ Ea273 \/ 27-3) UPF0255 protein EAMY_0885 Erwinia amylovora
erwbe-d8mnk7 Erwinia billingiae (strain Eb661) UPF0255 protein EbC_08830 Erwinia billingiae
erwct-Y3465 Erwinia carotovora (subsp. atroseptica) (Pectobacterium atrosepticum) ,Pectobacterium carotovorum subsp. carotovorum PC1) hypothetical protein Erwinia carotovora
erwpe-d0fv08 Erwinia pyrifoliae, Erwinia sp., UPF0255 protein EpC_27190 Erwinia pyrifoliae
erwt9-y2597 Erwinia tasmaniensis (strain DSM 17950 \/ Et1\/99) UPF0255 protein ETA_25970 Erwinia tasmaniensis
klep7-y243 Klebsiella pneumoniae, Klebsiella sp., Klebsiella variicola, UPF0255 protein KPN78578_02430 Klebsiella pneumoniae subsp. pneumoniae
lisda-d0yzd6 Photobacterium damselae subsp. damselae CIP 102761 UPF0255 protein VDA_002649 Photobacterium damselae subsp. damselae CIP 102761
mycvp-a1t3u4 Mycobacterium vanbaalenii (strain DSM 7251 \/ PYR-1) Putative uncharacterized protein Mycobacterium vanbaalenii
panam-d4gl10 Pantoea ananatis (strains LMG 20103\; AJ13355) UPF0255 protein PANA_0889 Pantoea ananatis
pansa-e6wam4 Pantoea sp. (strain At-9b) UPF0255 protein Pat9b_0825 Pantoea sp.
panvc-e1sek2 Pantoea vagans (strain C9-1) (Pantoea agglomerans (strain C9-1)), Pantoea sp. aB , UPF0255 protein Pvag_0279 Pantoea vagans
pecww-d0kkc9 Pectobacterium wasabiae (strain WPP163) UPF0255 protein Pecwa_3435 Pectobacterium wasabiae
phoaa-c7bhw5 Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 \/ 3105-77) (Xenorhabdus luminescens (strain 2)) UPF0255 protein PAU_03219 Photorhabdus asymbiotica subsp. asymbiotica
pholl-y1242 Photorhabdus luminescens (subsp. laumondii) hypothetical upf0255 protein plu1242 Photorhabdus luminescens
phomo-f2p681 Photobacterium leiognathi subsp. mandapamensis svers.1.1 UPF0255 protein PMSV_213 Photobacterium leiognathi subsp. mandapamensis svers.1.1
phopr-q1z7k1 Photobacterium profundum 3TCK hypothetical protein Photobacterium profundum 3TCK
phopr-y837 Photobacterium profundum (Photobacterium sp. (strain SS9)) hypothetical upf0255 protein pbpra0837 Photobacterium profundum
promh-b4euu8 Proteus mirabilis (strains HI4320\; ATCC 29906) Proteus penneri ATCC 35198 Esterase FrsA family protein Proteus mirabilis
prore-d4c4l5 Providencia rettgeri DSM 1131 Esterase FrsA Providencia rettgeri DSM 1131
prost-b2pu03 Providencia stuartii ATCC 25827 Putative uncharacterized protein Providencia stuartii ATCC 25827
psyin-a1syu2 Psychromonas ingrahamii (strain 37) Putative uncharacterized protein Psychromonas ingrahamii
rahsy-e8xpe3 Rahnella sp. (strain Y9602) UPF0255 protein Rahaq_0959 Rahnella sp.
salty-yafa Salmonella typhi, S. typhimurium, S. paratyphi, S. cholerae-suis, S. agona, S. arizonae, S. dublin, S. enteritidis, S. gallinarum, S. heidelberg, S. newport, S. schwarzengrund, Esterase frsA Esterase yafa (EC 3.1.-.-) Salmonella typhi
serod-d1rvl5 Serratia odorifera 4Rx13 UPF0255 protein SOD_e00790 Serratia odorifera 4Rx13
serod-d4dwl9 Serratia odorifera DSM 4582 UPF0255 protein frsA Serratia odorifera DSM 4582
serp5-y965 Serratia proteamaculans (strain 568) UPF0255 protein Spro_0965 Serratia proteamaculans
shifl-yafa Shigella flexneri, Shigella sonnei, Shigella boydii, Shigella dysenteriae, Shigella boydii, (EC 3.1.-.-) esterase yafa Fermentation-respiration switch protein frsA Shigella flexneri
sodgm-q2nvf1 Sodalis glossinidius (strain morsitans) hypothetical protein Sodalis glossinidius
tolat-c4lbk2 Tolumonas auensis (strain DSM 9187 \/ TA4) Putative uncharacterized protein Tolumonas auensis
uncma-q0w8x7 Uncultured methanogenic archaeon RC-I Uncharacterized protein Uncultured methanogenic archaeon RC-I
vibal-q1vai8 Vibrio alginolyticus, Vibrio sp. hypothetical protein Vibrio alginolyticus 12G01
vibch-y2276 Vibrio cholerae, Vibrio mimicus, Vibrio sp. hypothetical upf0255 protein vc2276 Vibrio cholerae
vibf1-q5e6w2 Vibrio fischeri (strains ATCC 700601 \/ ES114\; MJ11) alpha\/beta hydrolase Vibrio fischeri
vibfn-f0lug8 Vibrio furnissii (strains DSM 14383 \/ NCTC 11218\; CIP 102972) UPF0255 protein vfu_A01054 Vibrio furnissii
vibcb-a7mwv0 Vibrio harveyi, Putative uncharacterized protein Vibrio harveyi
griho-d0i7j9 Grimontia hollisae CIP 101886 Putative uncharacterized protein Grimontia hollisae CIP 101886
vibme-c9p2z6 Vibrio metschnikovii CIP 69.14 Putative uncharacterized protein Vibrio metschnikovii CIP 69.14
vibor-c9qmm3 Vibrio orientalis CIP 102891 = ATCC 33934, Vibrio parahaemolyticus 16 Fermentation\/respiration switch protein Vibrio orientalis CIP 102891 = ATCC 33934
vibpa-y674 Vibrio parahaemolyticus hypothetical upf0255 protein vp0674 Vibrio parahaemolyticus
vibsl-b7vjb4 Vibrio splendidus Putative esterase Vibrio splendidus
vibvy-y856 Vibrio vulnificus hypothetical FrsA upf0255 protein vv0856 Vibrio vulnificus
xenbs-d3v437 Xenorhabdus bovienii (strain SS-2004) UPF0255 protein XBJ1_3298 Xenorhabdus bovienii
xenna-d3v9t6 Xenorhabdus nematophila (strain ATCC 19061 \/ DSM 3370 \/ LMG 1036 \/ NCIB 9965 \/ AN6) UPF0255 protein yafA Xenorhabdus nematophila
yere8-y3204 Yersinia enterocolitica, Yersinia kristensenii, Yersinia aldovae, Yersinia intermedia, Yersinia rohdei, Yersinia mollaretii, Yersinia bercovieri, UPF0255 protein YE3204 Yersinia enterocolitica
yerpe-y3224 Yersinia pestis, Yersinia pseudotuberculosis, hypothetical upf0255 protein ypo3224\/y0964\/yp0709 Yersinia pestis
yerru-c4ujw7 Yersinia ruckeri ATCC 29473 UPF0255 protein yruck0001_22940 Yersinia ruckeri ATCC 29473
entcl-y1bd05 Enterobacter cloacae. UPF0255 protein P852_00729 Enterobacter cloacae

Gene_locus_frgt Name Species
9vibr-a6d9n5 Vibrio shilonii AK1 Fermentation\/respiration switch protein Vibrio shilonii AK1

References (6)

Title : Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase - Kellett_2013_Biochemistry_52_1842
Author(s) : Kellett WF , Brunk E , Desai BJ , Fedorov AA , Almo SC , Gerlt JA , Rothlisberger U , Richards NG
Ref : Biochemistry , 52 :1842 , 2013
Abstract :
PubMedSearch : Kellett_2013_Biochemistry_52_1842
PubMedID: 23452154
Gene_locus related to this paper: vibvy-y856

Title : Genetic manipulation of the Fusarium fujikuroi fusarin gene cluster yields insight into the complex regulation and fusarin biosynthetic pathway - Niehaus_2013_Chem.Biol_20_1055
Author(s) : Niehaus EM , Kleigrewe K , Wiemann P , Studt L , Sieber CM , Connolly LR , Freitag M , Guldener U , Tudzynski B , Humpf HU
Ref : Chemical Biology , 20 :1055 , 2013
Abstract :
PubMedSearch : Niehaus_2013_Chem.Biol_20_1055
PubMedID: 23932525
Gene_locus related to this paper: gibf5-fus2 , gibf5-fus5

Title : FrsA functions as a cofactor-independent decarboxylase to control metabolic flux - Lee_2011_Nat.Chem.Biol_7_434
Author(s) : Lee KJ , Jeong CS , An YJ , Lee HJ , Park SJ , Seok YJ , Kim P , Lee JH , Lee KH , Cha SS
Ref : Nat Chemical Biology , 7 :434 , 2011
Abstract :
PubMedSearch : Lee_2011_Nat.Chem.Biol_7_434
PubMedID: 21623357
Gene_locus related to this paper: vibvy-y856

Title : Structure and action of a C-C bond cleaving alpha\/beta-hydrolase involved in nicotine degradation - Schleberger_2007_J.Mol.Biol_367_409
Author(s) : Schleberger C , Sachelaru P , Brandsch R , Schulz GE
Ref : Journal of Molecular Biology , 367 :409 , 2007
Abstract :
PubMedSearch : Schleberger_2007_J.Mol.Biol_367_409
PubMedID: 17275835
Gene_locus related to this paper: artni-Q93NG6

Title : Hydrolytic polyketide shortening by ayg1p, a novel enzyme involved in fungal melanin biosynthesis - Fujii_2004_J.Biol.Chem_279_44613
Author(s) : Fujii I , Yasuoka Y , Tsai HF , Chang YC , Kwon-Chung KJ , Ebizuka Y
Ref : Journal of Biological Chemistry , 279 :44613 , 2004
Abstract :
PubMedSearch : Fujii_2004_J.Biol.Chem_279_44613
PubMedID: 15310761
Gene_locus related to this paper: aspfu-AYG1

Title : A novel fermentation\/respiration switch protein regulated by enzyme IIAGlc in Escherichia coli - Koo_2004_J.Biol.Chem_279_31613
Author(s) : Koo BM , Yoon MJ , Lee CR , Nam TW , Choe YJ , Jaffe H , Peterkofsky A , Seok YJ
Ref : Journal of Biological Chemistry , 279 :31613 , 2004
Abstract :
PubMedSearch : Koo_2004_J.Biol.Chem_279_31613
PubMedID: 15169777
Gene_locus related to this paper: vibvy-y856