Liu_2006_PLoS.Pathog_2_e29

Reference

Title : New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum - Liu_2006_PLoS.Pathog_2_e29
Author(s) : Liu F , Lu J , Hu W , Wang SY , Cui SJ , Chi M , Yan Q , Wang XR , Song HD , Xu XN , Wang JJ , Zhang XL , Zhang X , Wang ZQ , Xue CL , Brindley PJ , McManus DP , Yang PY , Feng Z , Chen Z , Han ZG
Ref : PLoS Pathog , 2 :e29 , 2006
Abstract :

Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay.

PubMedSearch : Liu_2006_PLoS.Pathog_2_e29
PubMedID: 16617374
Gene_locus related to this paper: schja-q5byv1

Related information

Gene_locus schja-q5byv1

Citations formats

Liu F, Lu J, Hu W, Wang SY, Cui SJ, Chi M, Yan Q, Wang XR, Song HD, Xu XN, Wang JJ, Zhang XL, Zhang X, Wang ZQ, Xue CL, Brindley PJ, McManus DP, Yang PY, Feng Z, Chen Z, Han ZG (2006)
New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum
PLoS Pathog 2 :e29

Liu F, Lu J, Hu W, Wang SY, Cui SJ, Chi M, Yan Q, Wang XR, Song HD, Xu XN, Wang JJ, Zhang XL, Zhang X, Wang ZQ, Xue CL, Brindley PJ, McManus DP, Yang PY, Feng Z, Chen Z, Han ZG (2006)
PLoS Pathog 2 :e29