| Title : Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper - Maki_2002_Biosci.Biotechnol.Biochem_66_1393 |
| Author(s) : Maki T , Kozawa H , Mima J , Ueno H , Hayashi R |
| Ref : Biosci Biotechnol Biochem , 66 :1393 , 2002 |
|
Abstract :
To investigate the structural importance of a "disulfide zipper" motif of carboxypeptidase Y, disulfide-deficient mutant enzymes were expressed in two strains of Saccharomyces cerevisiae. The mutant enzymes were rapidly degraded into fragments by intracellular proteases. Thus, it is concluded that the disulfide zipper is essential in maintaining the structural integrity of CPase Y against proteolytic susceptibility. |
| PubMedSearch : Maki_2002_Biosci.Biotechnol.Biochem_66_1393 |
| PubMedID: 12162566 |
| Gene_locus related to this paper: yeast-cbpy1 |
| Gene_locus | yeast-cbpy1 |
| Family | Carboxypeptidase_S10 |
| Structure | 1WPX |
Maki T, Kozawa H, Mima J, Ueno H, Hayashi R (2002)
Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper
Biosci Biotechnol Biochem
66 :1393
Maki T, Kozawa H, Mima J, Ueno H, Hayashi R (2002)
Biosci Biotechnol Biochem
66 :1393