Carboxypeptidase_S10

Relationship

Family: Carboxypeptidase_S10

Block: X

Parent Family: No family

Comment

Serine carboxypeptidase-like (SCPL) proteins. Structures determined for wheat and yeast peptidase first. Very successful family in plants (arabdopsis) also human protective protein cathepsineA. The protective protein human-CTSA is a glycoprotein that associates with lysosomal beta-galactosidase and neuraminidase and is deficient in the autosomal recessive disorder Galactosialidosis . Also contains non peptidase sequences as hydroxynitril lyase from plants. But new structure of sorghum hnl shows clear differences in catalytic mechanism because of a deletion of two amino acid adjescent of the active site serine: see sorbi-hnl and 1GXS Lauble et al 2002. It does not accept aliphatic hydroxynitriles, unlike EC 4.1.2.10 mandelonitrile lyase and EC 4.1.2.39 hydroxynitrilase. Other hydroxynitril lyase belong to another family: Hydroxynitrile_lyase . This Carboxypeptidase family also contains acyltransferases as sinapoyltransferase AT2G22990 arath-SCP8 SNG1 gene. Another enzyme, related to the SCPL family, is glucose acyltransferase from Lycopersicon pennellii catalysing the formation of diacylglucose. Some of these SCPL have a T instead of the serine in the catalytic triad: probably no catalytic activity but partner of another Serine carboxypeptitase-like protein.

Database

Interpro : IPR001563 Peptidase S10, serine carboxypeptidase

PIRSF : No PIRSF

Pdoc : PDOC00122

Pfam : PF00450 Peptidase_S10

Prints : PR00724

EC Number : 3.4.16.6 || 4.1.2.11

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

Structures (20)

Genes Proteins in Carboxypeptidase_S10 family (619)

Fragments of genes in Carboxypeptidase_S10 family (87)

Structures in Carboxypeptidase_S10 family (20)

Substrates in Carboxypeptidase_S10 family (4)

Inhibitors in Carboxypeptidase_S10 family (16)

References (21)

Title : Insights into acylation mechanisms: co-expression of serine carboxypeptidase-like acyltransferases and their non-catalytic companion paralogs - Yao_2022_Plant.J_111_117
Author(s) : Yao S , Liu Y , Zhuang J , Zhao Y , Dai X , Jiang C , Wang Z , Jiang X , Zhang S , Qian Y , Tai Y , Wang Y , Wang H , Xie DY , Gao L , Xia T
Ref : Plant J , 111 :117 , 2022
PubMedID: 35437852
Gene_locus related to this paper: dioka-dkSCPL1 , camsi-SCPL5 , camsi-SCPL4

Title : Proteolytic activation of human cathepsin A - Kolli_2014_J.Biol.Chem_289_11592
Author(s) : Kolli N , Garman SC
Ref : Journal of Biological Chemistry , 289 :11592 , 2014
PubMedID: 24599961
Gene_locus related to this paper: human-CTSA

Title : Crystal structure of cathepsin A, a novel target for the treatment of cardiovascular diseases - Schreuder_2014_Biochem.Biophys.Res.Commun_445_451
Author(s) : Schreuder HA , Liesum A , Kroll K , Bohnisch B , Buning C , Ruf S , Sadowski T
Ref : Biochemical & Biophysical Research Communications , 445 :451 , 2014
PubMedID: 24530914
Gene_locus related to this paper: human-CTSA

Title : Novel beta-amino acid derivatives as inhibitors of cathepsin A. - Ruf_2012_J.Med.Chem_55_7636
Author(s) : Ruf S , Buning C , Schreuder H , Horstick G , Linz W , Olpp T , Pernerstorfer J , Hiss K , Kroll K , Kannt A , Kohlmann M , Linz D , Hubschle T , Rutten H , Wirth K , Schmidt T , Sadowski T
Ref : Journal of Medicinal Chemistry , 55 :7636 , 2012
PubMedID: 22861813
Gene_locus related to this paper: human-CTSA

Title : Structure determinants and substrate recognition of serine carboxypeptidase-like acyltransferases from plant secondary metabolism - Stehle_2006_FEBS.Lett_580_6366
Author(s) : Stehle F , Brandt W , Milkowski C , Strack D
Ref : FEBS Letters , 580 :6366 , 2006
PubMedID: 17094968
Gene_locus related to this paper: arath-SCP19 , arath-SCP8

Title : Structure of the carboxypeptidase Y inhibitor IC in complex with the cognate proteinase reveals a novel mode of the proteinase-protein inhibitor interaction - Mima_2005_J.Mol.Biol_346_1323
Author(s) : Mima J , Hayashida M , Fujii T , Narita Y , Hayashi R , Ueda M , Hata Y
Ref : Journal of Molecular Biology , 346 :1323 , 2005
PubMedID: 15713484
Gene_locus related to this paper: yeast-cbpy1

Title : An expression and bioinformatics analysis of the Arabidopsis serine carboxypeptidase-like gene family - Fraser_2005_Plant.Physiol_138_1136
Author(s) : Fraser CM , Rider LW , Chapple C
Ref : Plant Physiol , 138 :1136 , 2005
PubMedID: 15908604
Gene_locus related to this paper: arath-AT4g30610 , arath-SCP25 , arath-SCP27 , arath-SCP16 , arath-SCP4 , arath-SCP7 , arath-SCP8 , arath-SCP11 , arath-SCP15 , arath-SCP18 , arath-SCP23 , arath-SCP26 , arath-SCP28 , arath-SCP32 , arath-SCP33 , arath-SCP36 , arath-SCP40 , arath-SCP44 , arath-SCPL6 , arath-SCPL34 , arath-SCP22

Title : Serine carboxypeptidase-like acyltransferases - Milkowski_2004_Phytochemistry_65_517
Author(s) : Milkowski C , Strack D
Ref : Phytochemistry , 65 :517 , 2004
PubMedID: 15003414
Gene_locus related to this paper: arath-SCP8

Title : Bifunctional indole-3-acetyl transferase catalyses synthesis and hydrolysis of indole-3-acetyl-myo-inositol in immature endosperm of Zea mays - Kowalczyk_2003_Physiol.Plant_119_165
Author(s) : Kowalczyk S , Jakubowska A , Zielieska E , Bandurski RS
Ref : Physiologica Plantarum , 119 :165 , 2003
PubMedID:
Gene_locus related to this paper: maize-b4fg76

Title : Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper - Maki_2002_Biosci.Biotechnol.Biochem_66_1393
Author(s) : Maki T , Kozawa H , Mima J , Ueno H , Hayashi R
Ref : Biosci Biotechnol Biochem , 66 :1393 , 2002
PubMedID: 12162566
Gene_locus related to this paper: yeast-cbpy1

Title : Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme - Lauble_2002_Biochemistry_41_12043
Author(s) : Lauble H , Miehlich B , Forster S , Wajant H , Effenberger F
Ref : Biochemistry , 41 :12043 , 2002
PubMedID: 12356304
Gene_locus related to this paper: sorbi-hnl

Title : The sng2 mutant of Arabidopsis is defective in the gene encoding the serine carboxypeptidase-like protein sinapoylglucose:choline sinapoyltransferase - Shirley_2001_Plant.J_28_83
Author(s) : Shirley AM , McMichael CM , Chapple C
Ref : Plant J , 28 :83 , 2001
PubMedID: 11696189
Gene_locus related to this paper: arath-SCP19

Title : An acyltransferase catalyzing the formation of diacylglucose is a serine carboxypeptidase-like protein - Li_2000_Proc.Natl.Acad.Sci.U.S.A_97_6902
Author(s) : Li AX , Steffens JC
Ref : Proc Natl Acad Sci U S A , 97 :6902 , 2000
PubMedID: 10829071
Gene_locus related to this paper: solbe-PLDP15 , solpn-q9lky6

Title : Peptide aldehyde complexes with wheat serine carboxypeptidase II: implications for the catalytic mechanism and substrate specificity - Bullock_1996_J.Mol.Biol_255_714
Author(s) : Bullock TL , Breddam K , Remington SJ
Ref : Journal of Molecular Biology , 255 :714 , 1996
PubMedID: 8636973
Gene_locus related to this paper: wheat-cbp02

Title : Crystallization of a soluble form of the Kex1p serine carboxypeptidase from Saccharomyces cerevisiae - Shilton_1996_Protein.Sci_5_395
Author(s) : Shilton BH , Li Y , Tessier D , Thomas DY , Cygler M
Ref : Protein Science , 5 :395 , 1996
PubMedID: 8745419
Gene_locus related to this paper: yeast-kex01

Title : Site-Directed Mutagenesis on (Serine) Carboxypeptidase Y from Yeast. The Significance of Thr60 and Met398 in Hydrolysis and Aminolysis Reactions - Sorensen_1995_J.Am.Chem.Soc_117_5944
Author(s) : Sorensen SB , Raaschou-Nielsen M , Mortensen UH , Remington SJ , Breddam K
Ref : Journal of the American Chemical Society , 117 :5944 , 1995
PubMedID:
Gene_locus related to this paper: yeast-cbpy1

Title : Three-dimensional structure of the human 'protective protein': structure of the precursor form suggests a complex activation mechanism - Rudenko_1995_Structure_3_1249
Author(s) : Rudenko G , Bonten E , d'Azzo A , Hol WG
Ref : Structure , 3 :1249 , 1995
PubMedID: 8591035
Gene_locus related to this paper: human-CTSA

Title : Structure of the complex of L-benzylsuccinate with wheat serine carboxypeptidase II at 2.0-A resolution - Bullock_1994_Biochemistry_33_11127
Author(s) : Bullock TL , Branchaud B , Remington SJ
Ref : Biochemistry , 33 :11127 , 1994
PubMedID: 7727364
Gene_locus related to this paper: wheat-cbp02

Title : 2.8-A structure of yeast serine carboxypeptidase - Endrizzi_1994_Biochemistry_33_11106
Author(s) : Endrizzi JA , Breddam K , Remington SJ
Ref : Biochemistry , 33 :11106 , 1994
PubMedID: 7727362
Gene_locus related to this paper: yeast-cbpy1

Title : Molecular cloning of hydroxynitrile lyase from Sorghum bicolor (L.). Homologies to serine carboxypeptidases - Wajant_1994_Plant.Mol.Biol_26_735
Author(s) : Wajant H , Mundry KW , Pfizenmaier K
Ref : Plant Mol Biol , 26 :735 , 1994
PubMedID: 7948927
Gene_locus related to this paper: sorbi-hnl

Title : Refined atomic model of wheat serine carboxypeptidase II at 2.2-A resolution - Liao_1992_Biochemistry_31_9796
Author(s) : Liao DI , Breddam K , Sweet RM , Bullock T , Remington SJ
Ref : Biochemistry , 31 :9796 , 1992
PubMedID: 1390755
Gene_locus related to this paper: wheat-cbp02