Morty_1999_J.Biol.Chem_274_26149

Reference

Title : Oligopeptidase B from Trypanosoma brucei, a new member of an emerging subgroup of serine oligopeptidases - Morty_1999_J.Biol.Chem_274_26149
Author(s) : Morty RE , Lonsdale-Eccles JD , Morehead J , Caler EV , Mentele R , Auerswald EA , Coetzer TH , Andrews NW , Burleigh BA
Ref : Journal of Biological Chemistry , 274 :26149 , 1999
Abstract :

Trypanosoma brucei contains a soluble serine oligopeptidase (OP-Tb) that is released into the host bloodstream during infection, where it has been postulated to participate in the pathogenesis of African trypanosomiasis. Here, we report the identification of a single copy gene encoding the T. brucei oligopeptidase and a homologue from the related trypanosomatid pathogen Leishmania major. The enzymes encoded by these genes belong to an emerging subgroup of the prolyl oligopeptidase family of serine hydrolases, referred to as oligopeptidase B. The trypanosomatid oligopeptidases share 70% amino acid sequence identity with oligopeptidase B from the intracellular pathogen Trypanosoma cruzi, which has a demonstrated role in mammalian host cell signaling and invasion. OP-Tb exhibited no activity toward the prolyl oligopeptidase substrate H-Gly-Pro-7-amido-4-methylcoumarin. Instead, it had activity toward substrates of trypsin-like enzymes, particularly those that have basic amino acids in both P(1) and P(2) (e.g. benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin k(cat)/K(m) = 529 s(-1) microM(-1)). The activity of OP-Tb was enhanced by reducing agents and by polyamines, suggesting that these agents may act as in vivo regulators of OP-Tb activity. This study provides the basis of the characterization of a novel subgroup of serine oligopeptidases from kinetoplastid protozoa with potential roles in pathogenesis.

PubMedSearch : Morty_1999_J.Biol.Chem_274_26149
PubMedID: 10473566
Gene_locus related to this paper: leima-OPB , trybr-PEPTB

Related information

Gene_locus leima-OPB    trybr-PEPTB
Family leima-OPB    trybr-PEPTB    S9N_PREPL_Peptidase_S9

Citations formats

Morty RE, Lonsdale-Eccles JD, Morehead J, Caler EV, Mentele R, Auerswald EA, Coetzer TH, Andrews NW, Burleigh BA (1999)
Oligopeptidase B from Trypanosoma brucei, a new member of an emerging subgroup of serine oligopeptidases
Journal of Biological Chemistry 274 :26149

Morty RE, Lonsdale-Eccles JD, Morehead J, Caler EV, Mentele R, Auerswald EA, Coetzer TH, Andrews NW, Burleigh BA (1999)
Journal of Biological Chemistry 274 :26149