leima-OPB

Leishmania major Leishmania mexicana, Leishmania donovan, L. amazonensis, L. braziliensis, L. infantum, L. donovani, oligopeptidase B (EC 3.4.21.83)

Comment

Inhibitors of OPB include: leupeptin (26nM),antipain (69 nM), water-soluble serine protease inhibitor 4-(2-aminoethyl)-benzenesulfonyl-fluoride (AEBSF) but not PMSF. Substrates include: (Bz-Arg-AMC, Z-Phe-Arg-AMC, Z-Arg-Arg-AMC, Boc-Gly-Arg-Arg-AMC, Z-Gly-Gly-Arg-AMC, Z-Gly-Pro-AMC the best are di-arginine). Other strains: Leishmania major, Leishmania mexicana (strain MHOM\/GT\/2001\/U1103), Leishmania donovani (strain BPK282A1), Leishmania amazonensis, Leishmania braziliensis Leishmania infantum, Leishmania donovani

Relationship

Family : S9N_PREPL_Peptidase_S9

Block : X

Position in NCBI Life Tree : Leishmania major

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Euglenozoa N E > Kinetoplastida N E > Trypanosomatidae N E > Leishmaniinae N E > Leishmania [genus] N E > Leishmania [subgenus] N E > Leishmania major species complex N E > Leishmania major N E

Molecular evidence

No mutation

No kinetic

No disease

No substrate

Sequence

Peptide

MSSDSSVAAS AQPPIAAKKP HRVKFGYVEG EDRGPNPMNP PRYREDPYFW MRDDDRKDPA VIEHLNKEKV YFQARSADIA QLRDDIYAEH ISHINEDDMS APYVYGKYRY YTREVKGKPY KIYCRVFTDK EPGDVAAEEV IIDVNQVAEG KAFCDVMEVK PAPPEHDLVA FSVDMSGNEV YTIEFKRISD PSQTIADKVS GTNGEIVWGP DHTSLFYVTK DETLRENKVW RHVMGKLQSE DVCLYEEHNP LFSAFMYKAA DTNTLCIGSQ SPETAEVHLL DLRKGNAHNT LEIVRPREKG VRYDVQMHGT SHLVILTNEG GAVNHKLLIA PRGQPSDWSH VLVDHSEDVF MESIAVRSNY LVVAGRRAGL TRIWTMMADS QDGVFQAGTG LREVVMEEPI FTVHLVESQM LEYEEPTFRM EYSSLATPNT WFDVSPQDTL APLVKVREVG GGFDAANYKV ERRFATAPDQ TKIPLSVVCH KDLDMSQPQP CMLYGYGSYG LSMDPQFSIQ HLPYCDRGMI FAIAHIRGGS ELGRAWYEIG AKYLTKRNTF SDFIAAAEFL VNAKLTTPSQ LACEGRSAGG LLMGAVLNMR PDLFKVALAG VPFVDVMTTM CDPSIPLTTG EWEEWGNPNE YKYYDYMLSY SPMDNVRAQE YPNIMVQCGL HDPRVAYWEP AKWVSKLREC KTDNNEILLN IDMESGHFSA KDRYKFWKES AIQQAFVCKH LKSTVRLLVR R

References (6)

Title : The oligopeptidase B of Leishmania regulates parasite enolase and immune evasion - Swenerton_2011_J.Biol.Chem_286_429
Author(s) : Swenerton RK , Zhang S , Sajid M , Medzihradszky KF , Craik CS , Kelly BL , McKerrow JH
Ref : Journal of Biological Chemistry , 286 :429 , 2011
PubMedID: 20961853
Gene_locus related to this paper: leima-OPB

Title : Crystal structure of Leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor - McLuskey_2010_J.Biol.Chem_285_39249
Author(s) : McLuskey K , Paterson NG , Bland ND , Isaacs NW , Mottram JC
Ref : Journal of Biological Chemistry , 285 :39249 , 2010
PubMedID: 20926390
Gene_locus related to this paper: leima-OPB

Title : Oligopeptidase B from L. amazonensis: molecular cloning, gene expression analysis and molecular model - de Matos Guedes_2007_Parasitol.Res_101_853
Author(s) : de Matos Guedes HL , Carneiro MP , de Oliveira Gomes DC , Rossi-Bergmanmn B , de Simone SG
Ref : Parasitol Res , 101 :853 , 2007
PubMedID: 17530480
Gene_locus related to this paper: leima-OPB , leima-q4qj45

Title : The genome of the kinetoplastid parasite, Leishmania major - Ivens_2005_Science_309_436
Author(s) : Ivens AC , Peacock CS , Worthey EA , Murphy L , Aggarwal G , Berriman M , Sisk E , Rajandream MA , Adlem E , Aert R , Anupama A , Apostolou Z , Attipoe P , Bason N , Bauser C , Beck A , Beverley SM , Bianchettin G , Borzym K , Bothe G , Bruschi CV , Collins M , Cadag E , Ciarloni L , Clayton C , Coulson RM , Cronin A , Cruz AK , Davies RM , De Gaudenzi J , Dobson DE , Duesterhoeft A , Fazelina G , Fosker N , Frasch AC , Fraser A , Fuchs M , Gabel C , Goble A , Goffeau A , Harris D , Hertz-Fowler C , Hilbert H , Horn D , Huang Y , Klages S , Knights A , Kube M , Larke N , Litvin L , Lord A , Louie T , Marra M , Masuy D , Matthews K , Michaeli S , Mottram JC , Muller-Auer S , Munden H , Nelson S , Norbertczak H , Oliver K , O'Neil S , Pentony M , Pohl TM , Price C , Purnelle B , Quail MA , Rabbinowitsch E , Reinhardt R , Rieger M , Rinta J , Robben J , Robertson L , Ruiz JC , Rutter S , Saunders D , Schafer M , Schein J , Schwartz DC , Seeger K , Seyler A , Sharp S , Shin H , Sivam D , Squares R , Squares S , Tosato V , Vogt C , Volckaert G , Wambutt R , Warren T , Wedler H , Woodward J , Zhou S , Zimmermann W , Smith DF , Blackwell JM , Stuart KD , Barrell B , Myler PJ
Ref : Science , 309 :436 , 2005
PubMedID: 16020728
Gene_locus related to this paper: leima-e9ady6 , leima-L2464.12 , leima-L2802.02 , leima-OPB , leima-q4fw33 , leima-q4fwg8 , leima-q4fwj0 , leima-q4fya7 , leima-q4q0a1 , leima-q4q0t5 , leima-q4q0v0 , leima-q4q1h9 , leima-q4q2c9 , leima-q4q4j7 , leima-q4q4t6 , leima-q4q5j1 , leima-q4q6e9 , leima-q4q7v8 , leima-q4q8a8 , leima-q4q9g9 , leima-q4q080 , leima-q4q398 , leima-q4q615 , leima-q4q819 , leima-q4q871 , leima-q4q942 , leima-q4qae7 , leima-q4qb85 , leima-q4qdz7 , leima-q4qe26 , leima-q4qe31 , leima-q4qe85 , leima-q4qe86 , leima-q4qe87 , leima-q4qe90 , leima-q4qec8 , leima-q4qgz4 , leima-q4qgz5 , leima-q4qhs0 , leima-q4qj45

Title : Oligopeptidase B from Trypanosoma brucei, a new member of an emerging subgroup of serine oligopeptidases - Morty_1999_J.Biol.Chem_274_26149
Author(s) : Morty RE , Lonsdale-Eccles JD , Morehead J , Caler EV , Mentele R , Auerswald EA , Coetzer TH , Andrews NW , Burleigh BA
Ref : Journal of Biological Chemistry , 274 :26149 , 1999
PubMedID: 10473566
Gene_locus related to this paper: leima-OPB , trybr-PEPTB