Naffin-Olivos_2017_Biochemistry_56_2304

Reference

Title : Structure Determination of Mycobacterium tuberculosis Serine Protease Hip1 (Rv2224c) - Naffin-Olivos_2017_Biochemistry_56_2304
Author(s) : Naffin-Olivos JL , Daab A , White A , Goldfarb NE , Milne AC , Liu D , Baikovitz J , Dunn BM , Rengarajan J , Petsko GA , Ringe D
Ref : Biochemistry , 56 :2304 , 2017
Abstract :

The Mycobacterium tuberculosis (Mtb) serine protease Hip1 (hydrolase important for pathogenesis; Rv2224c) promotes tuberculosis (TB) pathogenesis by impairing host immune responses through proteolysis of a protein substrate, Mtb GroEL2. The cell surface localization of Hip1 and its immunomodulatory functions make Hip1 a good drug target for new adjunctive immune therapies for TB. Here, we report the crystal structure of Hip1 to a resolution of 2.6 A and the kinetic studies of the enzyme against model substrates and the protein GroEL2. The structure shows a two-domain protein, one of which contains the catalytic residues that are the signature of a serine protease. Surprisingly, a threonine is located within the active site close enough to hydrogen bond with the catalytic residues Asp463 and His490. Mutation of this residue, Thr466, to alanine established its importance for function. Our studies provide insights into the structure of a member of a novel family of proteases. Knowledge of the Hip1 structure will aid in designing inhibitors that could block Hip1 activity.

PubMedSearch : Naffin-Olivos_2017_Biochemistry_56_2304
PubMedID: 28346784
Gene_locus related to this paper: myctu-ym24

Related information

Gene_locus myctu-ym24
Family myctu-ym24    Tiancimycin-TnmK-Tripeptidase-HIP
Structure myctu-ym24    Tiancimycin-TnmK-Tripeptidase-HIP    5UGQ    5UNO    5UOH

Citations formats

Naffin-Olivos JL, Daab A, White A, Goldfarb NE, Milne AC, Liu D, Baikovitz J, Dunn BM, Rengarajan J, Petsko GA, Ringe D (2017)
Structure Determination of Mycobacterium tuberculosis Serine Protease Hip1 (Rv2224c)
Biochemistry 56 :2304

Naffin-Olivos JL, Daab A, White A, Goldfarb NE, Milne AC, Liu D, Baikovitz J, Dunn BM, Rengarajan J, Petsko GA, Ringe D (2017)
Biochemistry 56 :2304