Nam_2009_Biochem.Biophys.Res.Commun_389_247

Reference

Title : The crystal structure of an HSL-homolog EstE5 complex with PMSF reveals a unique configuration that inhibits the nucleophile Ser144 in catalytic triads - Nam_2009_Biochem.Biophys.Res.Commun_389_247
Author(s) : Nam KH , Kim SJ , Priyadarshi A , Kim HS , Hwang KY
Ref : Biochemical & Biophysical Research Communications , 389 :247 , 2009
Abstract : The esterase/lipase family (EC 3.1.1.3/EC 3.1.1.1) represents a diverse group of hydrolases that catalyze the cleavage of ester bonds and are widely distributed in animals, plants and microorganisms. Among these enzymes, hormone-sensitive lipases, play a critical role in the regulation of rodent fat cell lipolysis and are regarded as adipose tissue-specific enzymes. Recently, we reported the structural and biological characterization of EstE5 from the metagenome library [K.H. Nam, M.Y. Kim, S.J. Kim, A. Priyadarshi, W.H. Lee, K.Y. Hwang, Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase, Biochem. Biophys. Res. Commun. 379 (2009) 553-556]. The structure of this protein revealed that it belongs to the HSL-family. Here, we report the inhibition of the activity of the HSL-homolog EstE5 protein as determined by the use of esterase/lipase inhibitors. Our results revealed that the EstE5 protein is significantly inhibited by PMSF. In addition, this is the first study to identify the crystal structures of EstE5-PMSF at 2.4 and 2.5A among the HSL-homolog structures. This structural configuration is similar to that adopted when serine proteases are inhibited by PMSF. The results presented here provide valuable information regarding the properties of the HSL-family.
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PubMedID: 19715665
Gene_locus related to this paper: 9bact-Q0GMU2

Related information

Gene_locus related to this paper: 9bact-Q0GMU2

Citations formats

Nam KH, Kim SJ, Priyadarshi A, Kim HS, Hwang KY (2009)
The crystal structure of an HSL-homolog EstE5 complex with PMSF reveals a unique configuration that inhibits the nucleophile Ser144 in catalytic triads
Biochemical & Biophysical Research Communications 389 :247

Nam KH, Kim SJ, Priyadarshi A, Kim HS, Hwang KY (2009)
Biochemical & Biophysical Research Communications 389 :247