| Title : Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry - Suzuki_2007_Biosci.Biotechnol.Biochem_71_811 |
| Author(s) : Suzuki Y , Miyazaki M , Ito E , Suzuki M , Yamashita T , Taira H , Suzuki A |
| Ref : Biosci Biotechnol Biochem , 71 :811 , 2007 |
|
Abstract :
Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to (83)Asn was a bisecting complex type, Galbeta1-4GlcNAcbeta1-2Manalpha1-3(Galbeta1-4GlcNAcbeta1-2Manalpha1-6)(GlcNAcbeta 1-4)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc. |
| PubMedSearch : Suzuki_2007_Biosci.Biotechnol.Biochem_71_811 |
| PubMedID: 17341822 |
| Gene_locus related to this paper: felca-CAUXIN |
| Gene_locus | felca-CAUXIN |
Suzuki Y, Miyazaki M, Ito E, Suzuki M, Yamashita T, Taira H, Suzuki A (2007)
Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry
Biosci Biotechnol Biochem
71 :811
Suzuki Y, Miyazaki M, Ito E, Suzuki M, Yamashita T, Taira H, Suzuki A (2007)
Biosci Biotechnol Biochem
71 :811