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References (8)

Title : The cholinergic system in the olfactory center of the terrestrial slug Limax - Matsuo_2014_J.Comp.Neurol_522_2951
Author(s) : Matsuo R , Kobayashi S , Wakiya K , Yamagishi M , Fukuoka M , Ito E
Ref : Journal of Comparative Neurology , 522 :2951 , 2014
Abstract : Acetylcholine plays various important roles in the central nervous system of invertebrates as well as vertebrates. In the olfactory center of the terrestrial slug Limax, the local field potential (LFP) oscillates, and the change in its oscillatory frequency is thought to correlate with the detection of odor that potentially changes an ongoing behavior of the animal. Acetylcholine is known to upregulate the frequency of the LFP oscillation, and is one of the candidates for the neurotransmitters that are involved in such higher cognitive functions. However, there have been no histological data on the cholinergic system in gastropods, nor are there data on the receptors that are responsible for the upregulation of the oscillatory frequency of LFP due to the lack of analytical tools (such as antibodies or cDNA sequence information on cholinergic system-related genes). Here we cloned the cDNAs of choline acetyltransferase (ChAT), acetylcholinesterase, vesicular acetylcholine transporter, and several nicotinic acetylcholine receptors (nAChRs), and investigated their localization in the brain of Limax. We also generated a polyclonal antibody against ChAT to examine its localization, and investigated pharmacologically the involvement of nAChRs in the LFP oscillation. Our data showed: 1) dense distribution of the neurons expressing mRNAs of ChAT and vesicular acetylcholine transporter in the olfactory center; 2) spatially unique expression patterns of different nAChRs in the olfactory center; 3) involvement of nAChRs in the upregulation of the oscillation; 4) localization of ChAT protein in nerve fibers and/or terminals; and 5) the presence of cholinergic nerves in the tentacles. J. Comp. Neurol. 522:2951-2966, 2014. (c) 2014 Wiley Periodicals, Inc.
ESTHER : Matsuo_2014_J.Comp.Neurol_522_2951
PubMedSearch : Matsuo_2014_J.Comp.Neurol_522_2951
PubMedID: 24523205

Title : Molecular mechanism of strigolactone perception by DWARF14 - Nakamura_2013_Nat.Commun_4_2613
Author(s) : Nakamura H , Xue YL , Miyakawa T , Hou F , Qin HM , Fukui K , Shi X , Ito E , Ito S , Park SH , Miyauchi Y , Asano A , Totsuka N , Ueda T , Tanokura M , Asami T
Ref : Nat Commun , 4 :2613 , 2013
Abstract : Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.
ESTHER : Nakamura_2013_Nat.Commun_4_2613
PubMedSearch : Nakamura_2013_Nat.Commun_4_2613
PubMedID: 24131983
Gene_locus related to this paper: orysj-Q10QA5

Title : Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids - Morita_2013_Genome.Announc_1_e0006413
Author(s) : Morita T , Koike H , Koyama Y , Hagiwara H , Ito E , Fukuoka T , Imura T , Machida M , Kitamoto D
Ref : Genome Announc , 1 :e0006413 , 2013
Abstract : The basidiomycetous yeast Pseudozyma antarctica T-34 is an excellent producer of mannosylerythritol lipids (MELs), members of the multifunctional extracellular glycolipids, from various feedstocks. Here, the genome sequence of P. antarctica T-34 was determined and annotated. Analysis of the sequence might provide insights into the properties of this yeast that make it superior for use in the production of functional glycolipids, leading to the further development of P. antarctica for industrial applications.
ESTHER : Morita_2013_Genome.Announc_1_e0006413
PubMedSearch : Morita_2013_Genome.Announc_1_e0006413
PubMedID: 23558529
Gene_locus related to this paper: canar-LipB , psea3-m9mfk8 , psea3-m9lyb4 , psea3-m9mhk2 , psea3-m9lth4 , psea3-m9mcb7 , psea3-m9lv13 , psea3-m9m1x3 , psea3-m9mf48 , psea3-m9m693 , psea3-m9mbv1 , psea3-m9lqi4

Title : Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes - Miyauchi_2008_J.Biosci.Bioeng_105_628
Author(s) : Miyauchi K , Sukda P , Nishida T , Ito E , Matsumoto Y , Masai E , Fukuda M
Ref : J Biosci Bioeng , 105 :628 , 2008
Abstract : A dibenzofuran (DF)-degrading bacterium, Nocardioides sp. DF412, was isolated and its genes responsible for the degradation of DF were cloned and characterized. The dfdA1A2A3A4 gene cluster coding for a ring hydroxylating dioxygenase was located on a plasmid, and the dfdBC gene cluster coding for a ring-cleavage dioxygenase and a following hydrolase respectively was located on the chromosome. Reverse transcription-PCR analysis revealed that the transcription of both gene clusters was induced in the presence of DF. These two gene clusters also conferred the activity of each enzyme to a host strain. These results strongly suggest that the clusters are responsible for the degradation of DF in strain DF412.
ESTHER : Miyauchi_2008_J.Biosci.Bioeng_105_628
PubMedSearch : Miyauchi_2008_J.Biosci.Bioeng_105_628
PubMedID: 18640602
Gene_locus related to this paper: 9acto-b0i4w7

Title : Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry - Suzuki_2007_Biosci.Biotechnol.Biochem_71_811
Author(s) : Suzuki Y , Miyazaki M , Ito E , Suzuki M , Yamashita T , Taira H , Suzuki A
Ref : Biosci Biotechnol Biochem , 71 :811 , 2007
Abstract : Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to (83)Asn was a bisecting complex type, Galbeta1-4GlcNAcbeta1-2Manalpha1-3(Galbeta1-4GlcNAcbeta1-2Manalpha1-6)(GlcNAcbeta 1-4)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc.
ESTHER : Suzuki_2007_Biosci.Biotechnol.Biochem_71_811
PubMedSearch : Suzuki_2007_Biosci.Biotechnol.Biochem_71_811
PubMedID: 17341822
Gene_locus related to this paper: felca-CAUXIN

Title : Distributions of gamma-aminobutyric acid immunoreactive and acetylcholinesterase-containing cells in the primary olfactory system in the terrestrial slug Limax marginatus - Ito_2003_Zoolog.Sci_20_1337
Author(s) : Ito I , Watanabe S , Kimura T , Kirino Y , Ito E
Ref : Zoolog Sci , 20 :1337 , 2003
Abstract : The tentacular ganglion, the primary olfactory system of terrestrial slugs, exhibits spontaneous oscillations with a spatial coherence. The digit-like extensions (digits) of the tentacular ganglion presumably house the cell bodies of the neurons underlying the oscillations. The present study was designed to identify the anatomical and physiological determinants of these oscillations with a special focus on whether the neurons located in the digits contribute to the coherent oscillations. We recorded field potentials from the spatially separated sites in the digits in the terrestrial slug Limax marginatus. We also simultaneously recorded tentacular nerve to monitor the coherent oscillations. The spatially separated regions in the digits oscillated at the same frequency as the tentacular nerve, indicating a single coherent activity. To study the neural networks underlying the coherent oscillations, we examined the distributions of acetylcholinesterase (AChE)-containing and gamma-aminobutyric acid immunoreactive (GABA-ir) neurons. AChE-containing and GABA-ir fibers were found to connect the neurons in a branch of the digits with those in other branches. We also used a vital staining technique with 1,1'-didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate to examine the projections of neurons in the digits. Large stained cells were detected in many branches of the digits after placing the dye on one of the cell masses located in right and left sides of the tentacular ganglion. They were detected in the cell masses and in many branches of the digits after placing the dye on a branch of the digits. Our results showed that the slug primary olfactory system has highly interconnected neural networks.
ESTHER : Ito_2003_Zoolog.Sci_20_1337
PubMedSearch : Ito_2003_Zoolog.Sci_20_1337
PubMedID: 14624031

Title : Acquisition of neuronal proteins during differentiation of NG108-15 cells - Tojima_2000_Neurosci.Res_37_153
Author(s) : Tojima T , Yamane Y , Takahashi M , Ito E
Ref : Neurosci Res , 37 :153 , 2000
Abstract : The differentiated type of neuroblastomaxglioma hybrid cell line, NG108-15, has widely been used in in vitro studies instead of primary-cultured neurons. Here we examined whether NG108-15 cells can be used as a model for studying the neuronal differentiation process. We compared the expression of neuronal proteins (neurofilament 200 (NF200), phosphorylated-NF200 (p-NF200), microtubule associated protein 2, synaptophysin, syntaxin 1, choline acetyltransferase, and acetylcholinesterase (AChE)) and a glial protein (vimentin) between undifferentiated and differentiated NG108-15 cells by immunocytochemistry and immunoblot analysis. The expression of all neuronal proteins, with the exception of NF200 and p-NF200, was positive in differentiated cells, but almost negative in undifferentiated cells. On the other hand, cytoskeletal intermediate filaments (NF200 and p-NF200) for neurons and that (vimentin) for glia were present in both undifferentiated and differentiated cells. Furthermore, a high expression of AChE mRNA was confirmed in differentiated cells by reverse transcription-PCR analysis. Our results showed that even though the expression of cytoskeletal filaments does not change during differentiation of NG108-15 cells, these cells during differentiation can serve as an appropriate tool for investigating and understanding the mechanisms involved in neuronal development and differentiation.
ESTHER : Tojima_2000_Neurosci.Res_37_153
PubMedSearch : Tojima_2000_Neurosci.Res_37_153
PubMedID: 10867177

Title : Long-term transformation of an inhibitory into an excitatory GABAergic synaptic response - Alkon_1992_Proc.Natl.Acad.Sci.U.S.A_89_11862
Author(s) : Alkon DL , Sanchez-Andres JV , Ito E , Oka K , Yoshioka T , Collin C
Ref : Proc Natl Acad Sci U S A , 89 :11862 , 1992
Abstract : For a constant membrane potential, a predominantly inhibitory GABAergic synaptic response is shown to undergo long-term transformation into an excitatory response after pairing of exogenous gamma-aminobutyric acid (GABA) with postsynaptic depolarization or pairing of pre- and postsynaptic stimulation. Current- and voltage-clamp experiments suggest that this synaptic transformation is due to a shift from a net increase of conductance to a net decrease of conductance in response to GABA. GABA-induced elevation of intracellular calcium is prolonged after the same stimulus pairing and may, therefore, contribute to this synaptic transformation via Ca(2+)-activated phosphorylation pathways. This synaptic transformation, which does not follow unpaired stimulus presentations, occurs in a neuronal compartment spatially separated from the soma, which also changes during stimulus pairing.
ESTHER : Alkon_1992_Proc.Natl.Acad.Sci.U.S.A_89_11862
PubMedSearch : Alkon_1992_Proc.Natl.Acad.Sci.U.S.A_89_11862
PubMedID: 1334550