Suzuki M

References (42)

Title : Microbial decomposition of biodegradable plastics on the deep-sea floor - Omura_2024_Nat.Commun_15_568
Author(s) : Omura T , Isobe N , Miura T , Ishii S , Mori M , Ishitani Y , Kimura S , Hidaka K , Komiyama K , Suzuki M , Kasuya KI , Nomaki H , Nakajima R , Tsuchiya M , Kawagucci S , Mori H , Nakayama A , Kunioka M , Kamino K , Iwata T
Ref : Nat Commun , 15 :568 , 2024
Abstract : Microbes can decompose biodegradable plastics on land, rivers and seashore. However, it is unclear whether deep-sea microbes can degrade biodegradable plastics in the extreme environmental conditions of the seafloor. Here, we report microbial decomposition of representative biodegradable plastics (polyhydroxyalkanoates, biodegradable polyesters, and polysaccharide esters) at diverse deep-sea floor locations ranging in depth from 757 to 5552 m. The degradation of samples was evaluated in terms of weight loss, reduction in material thickness, and surface morphological changes. Poly(L-lactic acid) did not degrade at either shore or deep-sea sites, while other biodegradable polyesters, polyhydroxyalkanoates, and polysaccharide esters were degraded. The rate of degradation slowed with water depth. We analysed the plastic-associated microbial communities by 16S rRNA gene amplicon sequencing and metagenomics. Several dominant microorganisms carried genes potentially encoding plastic-degrading enzymes such as polyhydroxyalkanoate depolymerases and cutinases/polyesterases. Analysis of available metagenomic datasets indicated that these microorganisms are present in other deep-sea locations. Our results confirm that biodegradable plastics can be degraded by the action of microorganisms on the deep-sea floor, although with much less efficiency than in coastal settings.
ESTHER : Omura_2024_Nat.Commun_15_568
PubMedSearch : Omura_2024_Nat.Commun_15_568
PubMedID: 38278791

Title : Synthesis of 2,8-Dioxabicyclo[3.3.1]nonane Derivatives and Their Neuroprotective Activities - Kamauchi_2024_Chem.Pharm.Bull.(Tokyo)_72_56
Author(s) : Kamauchi H , Takanashi A , Suzuki M , Izumi K , Takao K , Sugita Y
Ref : Chem Pharm Bull (Tokyo) , 72 :56 , 2024
Abstract : Twenty natural-product-like 2,8-dioxabicyclo[3.3.1]nonane derivatives were synthesized and their neuroprotective activities were tested using human monoamine oxidases (MAO) A and B and acetyl and butyryl cholinesterases (ChE). Compound 1s showed inhibitory activity for MAO-A, MAO-B and acetylcholinesterase (AChE) (IC(50) values 34.0, 2.3 and 11.0 microM, respectively). The inhibition mode of (-)-1s for MAO-B was investigated. Chiral HPLC of (+/-)-1s separated the enantiomers and (-)-1s showed MAO-B inhibitory activity. Molecular docking simulation of (-)-1s and MAO-B revealed the binding mode.
ESTHER : Kamauchi_2024_Chem.Pharm.Bull.(Tokyo)_72_56
PubMedSearch : Kamauchi_2024_Chem.Pharm.Bull.(Tokyo)_72_56
PubMedID: 38171905

Title : An exploratory, open-label, randomized, multicenter trial of hachimijiogan for mild Alzheimer's disease - Kainuma_2022_Front.Pharmacol_13_991982
Author(s) : Kainuma M , Ouma S , Kawakatsu S , Iritani O , Yamashita KI , Ohara T , Hirano S , Suda S , Hamano T , Hieda S , Yasui M , Yoshiiwa A , Shiota S , Hironishi M , Wada-Isoe K , Sasabayashi D , Yamasaki S , Murata M , Funakoshi K , Hayashi K , Shirafuji N , Sasaki H , Kajimoto Y , Mori Y , Suzuki M , Ito H , Ono K , Tsuboi Y
Ref : Front Pharmacol , 13 :991982 , 2022
Abstract : Background: Alzheimer's disease (AD) is a progressive neurodegeneration and is the most prevalent form of dementia. Intervention at an early stage is imperative. Although three acetylcholinesterase inhibitors (AChEIs) are currently approved for the treatment of mild AD, they are not sufficiently effective. Novel treatments for mild AD are of utmost importance. Objective: To assess the effectiveness of hachimijiogan (HJG), a traditional Japanese herbal medicine (Kampo), in the treatment of mild AD. Methods: This exploratory, open-label, randomized, multicenter trial enrolled patients with mild AD whose score on the Mini Mental State Examination (MMSE) was over 21points. All participants had been taking the same dosage of AChEI for more than 3 months. The participants were randomly assigned to an HJG group taking HJG extract 7.5 g/day in addition to AChEI or to a control group treated only with AChEI. The primary outcome was the change from baseline to 6 months post treatment initiation on the Alzheimer's Disease Assessment Scale-cognitive component- Japanese version(ADAS-Jcog). The secondary outcomes were change from baseline of the Instrumental Activity of Daily Life (IADL), Apathy scale, and Neuropsychiatric Inventory (NPI) -Q score. Results: Among the 77 enrollees, the data of 69(34 HJG and 35 control)were available for analysis. The difference in the change of ADAS-Jcog from baseline to 6 months of the HJG and control groups was 1.29 (90% Confidence interval (CI), -0.74 to 3.32 p = 0.293). In the subgroup analysis, the differences in the change from baseline to 3 and 6 months for women were 3.70 (90% CI ,0.50 to 6.91, p = 0.059) and 2.90 (90% CI,0.09 to 5.71, p = 0.090), respectively. For patients over 65 years, the difference at 3 months was 2.35 (90%CI, 0.01 to 4.68 p = 0.099). No significant differences were found between the HJG and control groups in IADL score, Apathy scale, or NPI-Q score. Conclusion: Although not conclusive, our data indicate that HJG has an adjuvant effect for acetylcholinesterase inhibitors and that it delays the deterioration of the cognitive dysfunction of mild Altzheimer's disease patients. Clinical Trial Registration: http://clinicaltrials.gov Japan Registry of clinical trials, identifier jRCTs 071190018.
ESTHER : Kainuma_2022_Front.Pharmacol_13_991982
PubMedSearch : Kainuma_2022_Front.Pharmacol_13_991982
PubMedID: 36313371

Title : Maize domestication phenotypes reveal strigolactone networks coordinating grain size evolution with kernel-bearing cupule architecture - Guan_2022_Plant.Cell__
Author(s) : Guan JC , Li C , Flint-Garcia S , Suzuki M , Wu S , Saunders JW , Dong L , Bouwmeester HJ , McCarty DR , Koch KE
Ref : Plant Cell , : , 2022
Abstract : The maize (Zea mays) ear represents one of the most striking domestication phenotypes in any crop species, with the cob conferring an exceptional yield advantage over the ancestral form of teosinte. Remodeling of the grain-bearing surface required profound developmental changes. However, the underlying mechanisms remain unclear and can only be partly attributed to the known domestication gene Teosinte glume architecture 1 (Tga1). Here we show that a more complete conversion involves strigolactones (SLs), and that these are prominent players not only in the Tga1 phenotype, but also other domestication features of the ear and kernel. Genetic combinations of a teosinte tga1 allele with three SL-related mutants progressively enhanced ancestral morphologies. The SL mutants, in addition to modulating the tga1 phenotype, also reshaped kernel-bearing pedicels and cupules in a teosinte-like manner. Genetic and molecular evidence are consistent with SL regulation of TGA1, including direct interaction of TGA1 with components of the SL-signaling system shown here to mediate TGA1 availability by sequestration. Roles of the SL network extend to enhancing maize seed size and, importantly, coordinating increased kernel growth with remodeling of protective maternal tissues. Collectively, our data show that SLs have central roles in releasing kernels from restrictive maternal encasement and coordinating other factors that increase kernel size, physical support, and their exposure on the grain-bearing surface.
ESTHER : Guan_2022_Plant.Cell__
PubMedSearch : Guan_2022_Plant.Cell__
PubMedID: 36573016

Title : Characterization of a poly(butylene adipate- co -terephthalate) hydrolase from the mesophilic actinobacteria Rhodococcus fascians - Soulenthone_2021_Polym.Degrad.Stab_184_109481
Author(s) : Soulenthone P , Tachibana Y , Suzuki M , Mizuno T , Ohta Y , Kasuya KI
Ref : Polymer Degradation and Stability , 154 :109481 , 2021
Abstract : Poly(butylene adipate- co -terephthalate) (PBAT) possesses excellent film-forming ability and biodegrad- ability. Therefore, it is considered to be a promising mulching film material that eliminates the need for recovery. In the applications that require PBAT degradation in the field after use, it is important to un- derstand the biodegradation mechanism at moderate temperatures. We have previously isolated from the soil the mesophilic actinobacteria Rhodococcus fascians NKCM2511 that biodegraded PBAT under moderate temperature conditions (20-30 C). In this study, to clarify the mechanism of PBAT degradation by the strain NKCM2511, a DNA fragment carrying the gene pbath Rf responsible for the PBAT degradation activity was cloned. The gene encoded a 216-amino-acid-long protein designated as PBATH Rf . Homology modeling revealed that PBATH Rf belongs to the alpha/ betahydrolase fold family, lacking the lid domain covering the active site. PBATH Rf degraded PBAT film at 30 C at the rate of 0.10 +/- 0.03 mg/cm 2 /d and was capable of degrad- ing several other aliphatic polyester films. Liquid chromatography revealed that PBATH Rf preferentially cleaved the ester bond between 1,4-butanediol and adipic acid rather than that between 1,4-butanediol and terephthalic acid (T). This characteristic of PBATH Rf may explain the low degradation rate of the aliphatic - aromatic copolyester PBAT, compared to the rate of degradation of aliphatic polyesters without T. In addition, liquid chromatography showed that PBATH Rf released T, mono(2-hydroxyethyl) terephthalic acid, and bis(2-hydroxybutyl) terephthalate from an amorphous poly(ethylene terephthalate) (PET) film. However, no significant change in the PET film surface after the treatment with PBATH Rf was found by scanning electron microscopy. This is the first report of an enzyme from the mesophilic actinobacteria Rhodococcus fascians that can hydrolyze various polyesters, including PBAT, and catalyze hydrolysis on the surface of an amorphous PET film. This study also provides insight into the biodegradation mechanism of PBAT in the actual field as it describes an enzyme from a naturally occurring organism that acts in the medium temperature range.
ESTHER : Soulenthone_2021_Polym.Degrad.Stab_184_109481
PubMedSearch : Soulenthone_2021_Polym.Degrad.Stab_184_109481
PubMedID:
Gene_locus related to this paper: rhofa-a0a7i8e2z4

Title : TAK-071, a novel M1 positive allosteric modulator with low cooperativity, improves cognitive function in rodents with few cholinergic side effects - Sako_2019_Neuropsychopharmacology_44_950
Author(s) : Sako Y , Kurimoto E , Mandai T , Suzuki A , Tanaka M , Suzuki M , Shimizu Y , Yamada M , Kimura H
Ref : Neuropsychopharmacology , 44 :950 , 2019
Abstract : The muscarinic M1 receptor (M1R) is a promising target for treating cognitive impairment associated with cholinergic deficits in disorders such as Alzheimer's disease and schizophrenia. We previously reported that cooperativity (alpha-value) was key to lowering the risk of diarrhea by M1R positive allosteric modulators (M1 PAMs). Based on this, we discovered a low alpha-value M1 PAM, TAK-071 (alpha-value: 199), and characterized TAK-071 using T-662 as a reference M1 PAM with high alpha-value of 1786. Both TAK-071 and T-662 were potent and highly selective M1 PAMs, with inflection points of 2.7 and 0.62 nM, respectively. However, T-662 but not TAK-071 augmented isolated ileum motility. TAK-071 and T-662 increased hippocampal inositol monophosphate production through M1R activation and improved scopolamine-induced cognitive deficits in rats at 0.3 and 0.1 mg/kg, respectively. TAK-071 and T-662 also induced diarrhea at 10 and 0.1 mg/kg, respectively, in rats. Thus, taking into consideration the fourfold lower brain penetration ratio of T-662, TAK-071 had a wider margin between cognitive improvement and diarrhea induction than T-662. Activation of M1R increases neural excitability via membrane depolarization, reduced afterhyperpolarization, and generation of afterdepolarization in prefrontal cortical pyramidal neurons. T-662 induced all three processes, whereas TAK-071 selectively induced afterdepolarization. Combining sub-effective doses of TAK-071, but not T-662, with an acetylcholinesterase inhibitor, significantly ameliorated scopolamine-induced cognitive deficits in rats. TAK-071 may therefore provide therapeutic opportunities for cognitive dysfunction related to cholinergic deficits or reduced M1R expression, while minimizing peripheral cholinergic side effects.
ESTHER : Sako_2019_Neuropsychopharmacology_44_950
PubMedSearch : Sako_2019_Neuropsychopharmacology_44_950
PubMedID: 30089885

Title : TAK-071, a muscarinic M1 receptor positive allosteric modulator, attenuates scopolamine-induced quantitative electroencephalogram power spectral changes in cynomolgus monkeys - Kurimoto_2019_PLoS.One_14_e0207969
Author(s) : Kurimoto E , Nakashima M , Kimura H , Suzuki M
Ref : PLoS ONE , 14 :e0207969 , 2019
Abstract : Activation of the muscarinic M1 receptor is a promising approach to improve cognitive deficits associated with cholinergic dysfunction in Alzheimer's disease, dementia with Lewy bodies, and schizophrenia. TAK-071 is an M1-selective positive allosteric modulator that improves cognitive deficits induced by scopolamine, a non-selective muscarinic receptor antagonist, with reduced side effects on gastrointestinal function in rats. In this study, we explored changes in quantitative electroencephalography (qEEG) power bands, with or without scopolamine challenge, as a non-invasive translational biomarker for the effect of TAK-071 in cynomolgus monkeys. Scopolamine has been reported to increase theta and delta power bands and decrease alpha power band in healthy volunteers. In line with the clinical observations, scopolamine (25-100 mug/kg, subcutaneous administration [s.c.]) increased theta and delta power bands in cynomolgus monkeys in a dose-dependent manner, whereas it had the opposite effect on alpha power band. The effects of TAK-071 on scopolamine (25 mug/kg, s.c.)-induced qEEG spectral changes were examined using an acetylcholinesterase inhibitor donepezil and a muscarinic M1/M4 receptor agonist xanomeline as comparative cholinomimetics. TAK-071 (0.3-3 mg/kg, oral administration [p.o.]), donepezil (3 mg/kg, p.o.), and xanomeline (1 mg/kg, s.c.) suppressed the scopolamine-induced increases in alpha, theta, and delta power bands. These results suggest that changes in specific qEEG power bands, in particular theta and delta power bands in the context of scopolamine challenge, could be used as translational biomarkers for the evaluation of TAK-071 in clinical studies.
ESTHER : Kurimoto_2019_PLoS.One_14_e0207969
PubMedSearch : Kurimoto_2019_PLoS.One_14_e0207969
PubMedID: 30856192

Title : Different pharmacological responses in late-life depression with subsequent dementia: a case supporting the reserve threshold theory -
Author(s) : Akechi T , Suzuki M , Hashimoto N , Yamada T , Yamada A , Nakaaki S
Ref : Psychogeriatrics , 17 :500 , 2017
PubMedID: 28332247

Title : The Gonium pectorale genome demonstrates co-option of cell cycle regulation during the evolution of multicellularity - Hanschen_2016_Nat.Commun_7_11370
Author(s) : Hanschen ER , Marriage TN , Ferris PJ , Hamaji T , Toyoda A , Fujiyama A , Neme R , Noguchi H , Minakuchi Y , Suzuki M , Kawai-Toyooka H , Smith DR , Sparks H , Anderson J , Bakaric R , Luria V , Karger A , Kirschner MW , Durand PM , Michod RE , Nozaki H , Olson BJ
Ref : Nat Commun , 7 :11370 , 2016
Abstract : The transition to multicellularity has occurred numerous times in all domains of life, yet its initial steps are poorly understood. The volvocine green algae are a tractable system for understanding the genetic basis of multicellularity including the initial formation of cooperative cell groups. Here we report the genome sequence of the undifferentiated colonial alga, Gonium pectorale, where group formation evolved by co-option of the retinoblastoma cell cycle regulatory pathway. Significantly, expression of the Gonium retinoblastoma cell cycle regulator in unicellular Chlamydomonas causes it to become colonial. The presence of these changes in undifferentiated Gonium indicates extensive group-level adaptation during the initial step in the evolution of multicellularity. These results emphasize an early and formative step in the evolution of multicellularity, the evolution of cell cycle regulation, one that may shed light on the evolutionary history of other multicellular innovations and evolutionary transitions.
ESTHER : Hanschen_2016_Nat.Commun_7_11370
PubMedSearch : Hanschen_2016_Nat.Commun_7_11370
PubMedID: 27102219
Gene_locus related to this paper: gonpe-a0a150g7b7 , gonpe-a0a150g7j0 , gonpe-a0a150fxi1 , gonpe-a0a150gb26 , gonpe-a0a150grd7 , gonpe-a0a150gvy9 , gonpe-a0a150h2v5

Title : Correlation between hepatic blood flow and liver function in alcoholic liver cirrhosis - Takahashi_2014_World.J.Gastroenterol_20_17065
Author(s) : Takahashi H , Shigefuku R , Yoshida Y , Ikeda H , Matsunaga K , Matsumoto N , Okuse C , Sase S , Itoh F , Suzuki M
Ref : World J Gastroenterol , 20 :17065 , 2014
Abstract : AIM: To elucidate the correlation between hepatic blood flow and liver function in alcoholic liver cirrhosis (AL-LC).
METHODS: The subjects included 35 patients with AL-LC (34 men, 1 woman; mean age, 58.9 +/- 10.7 years; median age, 61 years; range: 37-76 years). All patients were enrolled in this study after obtaining written informed consent. Liver function was measured with tests measuring albumin (Alb), prothrombin time (PT), brain natriuretic peptide (BNP), branched amino acid and tyrosine ratio (BTR), branched chain amino acid (BCAA), tyrosine, ammonia (NH3), cholinesterase (ChE), immunoreactive insulin (IRI), total bile acid (TBA), and the retention rate of indocyanine green 15 min after administration (ICG R15). Hepatic blood flow, hepatic arterial tissue blood flow (HATBF), portal venous tissue blood flow (PVTBF), and total hepatic tissue blood flow (THTBF) were simultaneously calculated using xenon computed tomography.
RESULTS: PVTBF, HATBF and THTBF were 30.2 +/- 10.4, 20.0 +/- 10.7, and 50.3 +/- 14.9 mL/100 mL/min, respectively. Alb, PT, BNP, BTR, BCAA, tyrosine, NH3, ChE, IRI, TBA, and ICG R15 were 3.50 +/- 0.50 g/dL, 72.0% +/- 11.5%, 63.2 +/- 56.7 pg/mL, 4.06 +/- 1.24, 437.5 +/- 89.4 mumol/L, 117.7 +/- 32.8 mumol/L, 59.4 +/- 22.7 mug/dL, 161.0 +/- 70.8 IU/L, 12.8 +/- 5.0 mug/dL, 68.0 +/- 51.8 mumol/L, and 28.6% +/- 13.5%, respectively. PVTBF showed a significant negative correlation with ICG R15 (r = -0.468, P <0.01). No significant correlation was seen between ICG 15R, HATBF and THTBF. There was a significant correlation between PVTBF and Alb (r = 0.2499, P < 0.05), and NH3 tended to have an inverse correlation with PVTBF (r = -0.2428, P = 0.0894). There were also many significant correlations between ICG R15 and liver function parameters, including Alb, NH3, PT, BNP, TBA, BCAA, and tyrosine (r = -0.2156, P < 0.05; r = 0.4318, P < 0.01; r = 0.4140, P < 0.01; r = 0.3610, P < 0.05; r = 0.5085, P < 0.001; r = 0.4496, P < 0.01; and r = 0.4740, P < 0.05, respectively). CONCLUSION: Our investigation showed that there is a close correlation between liver function and hepatic blood flow.
ESTHER : Takahashi_2014_World.J.Gastroenterol_20_17065
PubMedSearch : Takahashi_2014_World.J.Gastroenterol_20_17065
PubMedID: 25493018

Title : Molecular epidemiology and clinical spectrum of hereditary spastic paraplegia in the Japanese population based on comprehensive mutational analyses - Ishiura_2014_J.Hum.Genet_59_163
Author(s) : Ishiura H , Takahashi Y , Hayashi T , Saito K , Furuya H , Watanabe M , Murata M , Suzuki M , Sugiura A , Sawai S , Shibuya K , Ueda N , Ichikawa Y , Kanazawa I , Goto J , Tsuji S
Ref : J Hum Genet , 59 :163 , 2014
Abstract : Hereditary spastic paraplegia (HSP) is one of the most genetically heterogeneous neurodegenerative disorders characterized by progressive spasticity and pyramidal weakness of lower limbs. Because >30 causative genes have been identified, screening of multiple genes is required for establishing molecular diagnosis of individual patients with HSP. To elucidate molecular epidemiology of HSP in the Japanese population, we have conducted mutational analyses of 16 causative genes of HSP (L1CAM, PLP1, ATL1, SPAST, CYP7B1, NIPA1, SPG7, KIAA0196, KIF5A, HSPD1, BSCL2, SPG11, SPG20, SPG21, REEP1 and ZFYVE27) using resequencing microarrays, array-based comparative genomic hybridization and Sanger sequencing. The mutational analysis of 129 Japanese patients revealed 49 mutations in 46 patients, 32 of which were novel. Molecular diagnosis was accomplished for 67.3% (33/49) of autosomal dominant HSP patients. Even among sporadic HSP patients, mutations were identified in 11.1% (7/63) of them. The present study elucidated the molecular epidemiology of HSP in the Japanese population and further broadened the mutational and clinical spectra of HSP.
ESTHER : Ishiura_2014_J.Hum.Genet_59_163
PubMedSearch : Ishiura_2014_J.Hum.Genet_59_163
PubMedID: 24451228
Gene_locus related to this paper: human-SPG21

Title : Draft Genome Sequence of Helicobacter fennelliae Strain MRY12-0050, Isolated from a Bacteremia Patient - Rimbara_2013_Genome.Announc_1_e00512
Author(s) : Rimbara E , Matsui M , Mori S , Suzuki S , Suzuki M , Kim H , Sekizuka T , Kuroda M , Shibayama K
Ref : Genome Announc , 1 : , 2013
Abstract : Helicobacter fennelliae, a human enterohepatic pathogen, causes bacteremia and colitis. We isolated H. fennelliae strain MRY12-0050 from a female patient; this strain was isolated from 2 other patients from the same hospital during the same period, suggesting human-to-human transmission. This is the first report of an H. fennelliae genome sequence.
ESTHER : Rimbara_2013_Genome.Announc_1_e00512
PubMedSearch : Rimbara_2013_Genome.Announc_1_e00512
PubMedID: 23929465

Title : Distinct localization of peripheral and central types of choline acetyltransferase in the rat cochlea - Kitanishi_2013_Acta.Histochem.Cytochem_46_145
Author(s) : Kitanishi T , Aimi Y , Kitano H , Suzuki M , Kimura H , Saito A , Shimizu T , Tooyama I
Ref : Acta Histochemica Cytochem , 46 :145 , 2013
Abstract : We previously discovered a splice variant of choline acetyltransferase (ChAT) mRNA, and designated the variant protein pChAT because of its preferential expression in peripheral neuronal structures. In this study, we examined the immunohistochemical localization of pChAT in rat cochlea and compared the distribution pattern to those of common ChAT (cChAT) and acetylcholinesterase. Some neuronal cell bodies and fibers in the spiral ganglia showed immunoreactivity for pChAT, predominantly the small spiral ganglion cells, indicating outer hair cell type II neurons. In contrast, cChAT- and acetylcholinesterase-positive structures were localized to fibers and not apparent in ganglion cells. After ablation of the cochlear nuclei, many pChAT-positive cochlear nerve fibers became clearly visible, whereas fibers immunopositive for cChAT and acetylcholine esterase disappeared. These results suggested that pChAT and cChAT are localized in different systems of the rat cochlea; pChAT in the afferent and cChAT in the efferent structures.
ESTHER : Kitanishi_2013_Acta.Histochem.Cytochem_46_145
PubMedSearch : Kitanishi_2013_Acta.Histochem.Cytochem_46_145
PubMedID: 24194628

Title : Rivastigmine dermal patch solves eating problems in an individual with advanced Alzheimer's disease -
Author(s) : Uwano C , Suzuki M , Aikawa T , Ebihara T , Une K , Tomita N , Kosaka Y , Okinaga S , Furukawa K , Arai H , Ohrui T
Ref : J Am Geriatr Soc , 60 :1979 , 2012
PubMedID: 23057453

Title : Polyphenols extracted from black tea (Camellia sinensis) residue by hot-compressed water and their inhibitory effect on pancreatic lipase in vitro - Yuda_2012_J.Food.Sci_77_H254
Author(s) : Yuda N , Tanaka M , Suzuki M , Asano Y , Ochi H , Iwatsuki K
Ref : J Food Sci , 77 :H254 , 2012
Abstract : Polyphenols, retained in black tea wastes following the commercial production of tea beverages, represent an underutilized resource. The purpose of this study was to investigate the potential use of hot-compressed water (HCW) for the extraction of pancreatic lipase-inhibiting polyphenols from black tea residues. Black tea residues were treated with HCW at 10 degreesC intervals, from 100 to 200 degreesC. The resulting extracts were analyzed using high-performance liquid chromatography-mass spectrometry and assayed to determine their inhibitory effect on pancreatic lipase activity in vitro. Four theaflavins (TF), 5 catechins, 2 quercetin glycosides, quinic acid, gallic acid, and caffeine were identified. The total polyphenol content of extracts increased with increasing temperature but lipase inhibitors (TF, theaflavin 3-O-gallate, theaflavin 3'-O-gallate, theaflavin 3,3'-O-gallate, epigallocatechin gallate, and epicatechin gallate) decreased over 150 degreesC. All extracts inhibited pancreatic lipase but extracts obtained at 100 to 140 degreesC showed the greatest lipase inhibition (IC(50) s of 0.9 to 1.3 microg/mL), consistent with the optimal extraction of TFs and catechins except catechin by HCW between 130 and 150 degreesC. HCW can be used to extract pancreatic lipase-inhibiting polyphenols from black tea waste. These extracts have potential uses, as dietary supplements and medications, for the prevention and treatment of obesity.
ESTHER : Yuda_2012_J.Food.Sci_77_H254
PubMedSearch : Yuda_2012_J.Food.Sci_77_H254
PubMedID: 23106349

Title : Diverse roles of strigolactone signaling in maize architecture and the uncoupling of a branching-specific subnetwork - Guan_2012_Plant.Physiol_160_1303
Author(s) : Guan JC , Koch KE , Suzuki M , Wu S , Latshaw S , Petruff T , Goulet C , Klee HJ , McCarty DR
Ref : Plant Physiol , 160 :1303 , 2012
Abstract : Strigolactones (SLs) control lateral branching in diverse species by regulating transcription factors orthologous to Teosinte branched1 (Tb1). In maize (Zea mays), however, selection for a strong central stalk during domestication is attributed primarily to the Tb1 locus, leaving the architectural roles of SLs unclear. To determine how this signaling network is altered in maize, we first examined effects of a knockout mutation in an essential SL biosynthetic gene that encodes CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8), then tested interactions between SL signaling and Tb1. Comparative genome analysis revealed that maize depends on a single CCD8 gene (ZmCCD8), unlike other panicoid grasses that have multiple CCD8 paralogs. Function of ZmCCD8 was confirmed by transgenic complementation of Arabidopsis (Arabidopsis thaliana) max4 (ccd8) and by phenotypic rescue of the maize mutant (zmccd8::Ds) using a synthetic SL (GR24). Analysis of the zmccd8 mutant revealed a modest increase in branching that contrasted with prominent pleiotropic changes that include (1) marked reduction in stem diameter, (2) reduced elongation of internodes (independent of carbon supply), and (3) a pronounced delay in development of the centrally important, nodal system of adventitious roots. Analysis of the tb1 zmccd8 double mutant revealed that Tb1 functions in an SL-independent subnetwork that is not required for the other diverse roles of SL in development. Our findings indicate that in maize, uncoupling of the Tb1 subnetwork from SL signaling has profoundly altered the balance between conserved roles of SLs in branching and diverse aspects of plant architecture.
ESTHER : Guan_2012_Plant.Physiol_160_1303
PubMedSearch : Guan_2012_Plant.Physiol_160_1303
PubMedID: 22961131

Title : Iodide oxidation by a novel multicopper oxidase from the alphaproteobacterium strain Q-1 - Suzuki_2012_Appl.Environ.Microbiol_78_3941
Author(s) : Suzuki M , Eda Y , Ohsawa S , Kanesaki Y , Yoshikawa H , Tanaka K , Muramatsu Y , Yoshikawa J , Sato I , Fujii T , Amachi S
Ref : Applied Environmental Microbiology , 78 :3941 , 2012
Abstract : Alphaproteobacterium strain Q-1 is able to oxidize iodide (I(-)) to molecular iodine (I(2)) by an oxidase-like enzyme. One of the two isoforms of the iodide-oxidizing enzyme (IOE-II) produced by this strain was excised from a native polyacrylamide gel, eluted, and purified. IOE-II appeared as a single band (51 kDa) and showed significant in-gel iodide-oxidizing activity in sodium dodecyl sulfate-polyacrylamide gel electrophoresis without heat treatment. However, at least two bands with much higher molecular masses (150 and 230 kDa) were observed with heat treatment (95 degrees C, 3 min). IOE-II was inhibited by NaN(3), KCN, EDTA, and a copper chelator, o-phenanthroline. In addition to iodide, IOE-II showed significant activities toward phenolic compounds such as syringaldazine, 2,6-dimethoxy phenol, and p-phenylenediamine. IOE-II contained copper atoms as prosthetic groups and had UV/VIS absorption peaks at 320 and 590 nm. Comparison of several internal amino acid sequences obtained from trypsin-digested IOE-II with a draft genome sequence of strain Q-1 revealed that the products of two open reading frames (IoxA and IoxC), with predicted molecular masses of 62 and 71 kDa, are involved in iodide oxidation. Furthermore, subsequent tandem mass spectrometric analysis repeatedly detected peptides from IoxA and IoxC with high sequence coverage (32 to 40%). IoxA showed homology with the family of multicopper oxidases and included four copper-binding regions that are highly conserved among various multicopper oxidases. These results suggest that IOE-II is a multicopper oxidase and that it may occur as a multimeric complex in which at least two proteins (IoxA and IoxC) are associated.
ESTHER : Suzuki_2012_Appl.Environ.Microbiol_78_3941
PubMedSearch : Suzuki_2012_Appl.Environ.Microbiol_78_3941
PubMedID: 22447601
Gene_locus related to this paper: 9prot-a0a061qis2 , 9prot-a0a061q8k2 , 9prot-a0a061qhj5

Title : Identification of substrain-specific mutations by massively parallel whole-genome resequencing of Synechocystis sp. PCC 6803 - Kanesaki_2012_DNA.Res_19_67
Author(s) : Kanesaki Y , Shiwa Y , Tajima N , Suzuki M , Watanabe S , Sato N , Ikeuchi M , Yoshikawa H
Ref : DNA Research , 19 :67 , 2012
Abstract : The cyanobacterium, Synechocystis sp. PCC 6803, was the first photosynthetic organism whose genome sequence was determined in 1996 (Kazusa strain). It thus plays an important role in basic research on the mechanism, evolution, and molecular genetics of the photosynthetic machinery. There are many substrains or laboratory strains derived from the original Berkeley strain including glucose-tolerant (GT) strains. To establish reliable genomic sequence data of this cyanobacterium, we performed resequencing of the genomes of three substrains (GT-I, PCC-P, and PCC-N) and compared the data obtained with those of the original Kazusa strain stored in the public database. We found that each substrain has sequence differences some of which are likely to reflect specific mutations that may contribute to its altered phenotype. Our resequence data of the PCC substrains along with the proposed corrections/refinements of the sequence data for the Kazusa strain and its derivatives are expected to contribute to investigations of the evolutionary events in the photosynthetic and related systems that have occurred in Synechocystis as well as in other cyanobacteria.
ESTHER : Kanesaki_2012_DNA.Res_19_67
PubMedSearch : Kanesaki_2012_DNA.Res_19_67
PubMedID: 22193367
Gene_locus related to this paper: synsp-ester

Title : Depot-specific expression of lipolytic genes in human adipose tissues--association among CES1 expression, triglyceride lipase activity and adiposity - Nagashima_2011_J.Atheroscler.Thromb_18_190
Author(s) : Nagashima S , Yagyu H , Takahashi N , Kurashina T , Takahashi M , Tsuchita T , Tazoe F , Wang XL , Bayasgalan T , Sato N , Okada K , Nagasaka S , Gotoh T , Kojima M , Hyodo M , Horie H , Hosoya Y , Okada M , Yasuda Y , Fujiwara H , Ohwada M , Iwamoto S , Suzuki M , Nagai H , Ishibashi S
Ref : J Atheroscler Thromb , 18 :190 , 2011
Abstract : AIM: Adipocyte lipolysis is mediated by a family of triglyceride (TG) lipases consisting of hormone-sensitive lipase (LIPE), adipose triglyceride lipase (PNPLA2) and carboxylesterase 1 (CES1); however, little is known about the relationship between the expression of each gene in different depots and TG lipase activity or obesity. METHOD: We measured both mRNA expression levels of the lipolytic enzymes (LIPE, PNPLA2 and CES1) and TG lipase activities of biopsy samples obtained from subcutaneous, omental and mesenteric adipose tissues of 34 patients who underwent abdominal surgery. The results were correlated with clinical parameters: adiposity measures, parameters for insulin resistance and plasma lipid levels. RESULTS: PNPLA2 mRNA levels were slightly higher in omental fat than subcutaneous fat. Cytosolic TG lipase activities were positively correlated with the mRNA levels of CES1 in subcutaneous fat and mesenteric fat, while they were correlated with those of PNPLA2 in omental fat. The mRNA levels of LIPE were negatively correlated with various measures of adiposity in subcutaneous fat. The mRNA levels of CES1 were positively correlated with various measures of adiposity, particularly those estimated by CT in the three depots; they were also positively correlated with plasma LDL-cholesterol levels in omental fat. In contrast, the mRNA levels of PNPLA2 were not significantly associated with adiposity. CONCLUSIONS: The positive correlations of the expression of CES1 with cytosolic TG lipase activities as well as with adiposity suggest that CES1 is involved in lipolysis, thereby contributing to the development of obesity-associated phenotypes. On the other hand, the expression of LIPE is negatively correlated with adiposity. These distinct regulatory patterns of lipolytic genes may underlie the complex phenotypes associated with human obesity.
ESTHER : Nagashima_2011_J.Atheroscler.Thromb_18_190
PubMedSearch : Nagashima_2011_J.Atheroscler.Thromb_18_190
PubMedID: 21081832

Title : The molecular and cellular identity of peripheral osmoreceptors - Lechner_2011_Neuron_69_332
Author(s) : Lechner SG , Markworth S , Poole K , Smith ES , Lapatsina L , Frahm S , May M , Pischke S , Suzuki M , Ibanez-Tallon I , Luft FC , Jordan J , Lewin GR
Ref : Neuron , 69 :332 , 2011
Abstract : In mammals, the osmolality of the extracellular fluid (ECF) is highly stable despite radical changes in salt/water intake and excretion. Afferent systems are required to detect hypo- or hyperosmotic shifts in the ECF to trigger homeostatic control of osmolality. In humans, a pressor reflex is triggered by simply drinking water which may be mediated by peripheral osmoreceptors. Here, we identified afferent neurons in the thoracic dorsal root ganglia (DRG) of mice that innervate hepatic blood vessels and detect physiological hypo-osmotic shifts in blood osmolality. Hepatic sensory neurons are equipped with an inward current that faithfully transduces graded changes in osmolality within the physiological range (~15 mOsm). In mice lacking the osmotically activated ion channel, TRPV4, hepatic sensory neurons no longer exhibit osmosensitive inward currents and activation of peripheral osmoreceptors in vivo is abolished. We have thus identified a new population of sensory neurons that transduce ongoing changes in hepatic osmolality.
ESTHER : Lechner_2011_Neuron_69_332
PubMedSearch : Lechner_2011_Neuron_69_332
PubMedID: 21262470

Title : Crystal structure and enhanced activity of a cutinase-like enzyme from Cryptococcus sp. strain S-2 - Kodama_2009_Proteins_77_710
Author(s) : Kodama Y , Masaki K , Kondo H , Suzuki M , Tsuda S , Nagura T , Shimba N , Suzuki E , Iefuji H
Ref : Proteins , 77 :710 , 2009
Abstract : The structural and enzymatic characteristics of a cutinase-like enzyme (CLE) from Cryptococcus sp. strain S-2, which exhibits remote homology to a lipolytic enzyme and a cutinase from the fungus Fusarium solani (FS cutinase), were compared to investigate the unique substrate specificity of CLE. The crystal structure of CLE was solved to a 1.05 A resolution. Moreover, hydrolysis assays demonstrated the broad specificity of CLE for short and long-chain substrates, as well as the preferred specificity of FS cutinase for short-chain substrates. In addition, site-directed mutagenesis was performed to increase the hydrolysis activity on long-chain substrates, indicating that the hydrophobic aromatic residues are important for the specificity to the long-chain substrate. These results indicate that hydrophobic residues, especially the aromatic ones exposed to solvent, are important for retaining lipase activity.
ESTHER : Kodama_2009_Proteins_77_710
PubMedSearch : Kodama_2009_Proteins_77_710
PubMedID: 19544571
Gene_locus related to this paper: crysp-Q874E9

Title : The Rice Annotation Project Database (RAP-DB): 2008 update - Tanaka_2008_Nucleic.Acids.Res_36_D1028
Author(s) : Tanaka T , Antonio BA , Kikuchi S , Matsumoto T , Nagamura Y , Numa H , Sakai H , Wu J , Itoh T , Sasaki T , Aono R , Fujii Y , Habara T , Harada E , Kanno M , Kawahara Y , Kawashima H , Kubooka H , Matsuya A , Nakaoka H , Saichi N , Sanbonmatsu R , Sato Y , Shinso Y , Suzuki M , Takeda J , Tanino M , Todokoro F , Yamaguchi K , Yamamoto N , Yamasaki C , Imanishi T , Okido T , Tada M , Ikeo K , Tateno Y , Gojobori T , Lin YC , Wei FJ , Hsing YI , Zhao Q , Han B , Kramer MR , McCombie RW , Lonsdale D , O'Donovan CC , Whitfield EJ , Apweiler R , Koyanagi KO , Khurana JP , Raghuvanshi S , Singh NK , Tyagi AK , Haberer G , Fujisawa M , Hosokawa S , Ito Y , Ikawa H , Shibata M , Yamamoto M , Bruskiewich RM , Hoen DR , Bureau TE , Namiki N , Ohyanagi H , Sakai Y , Nobushima S , Sakata K , Barrero RA , Souvorov A , Smith-White B , Tatusova T , An S , An G , S OO , Fuks G , Messing J , Christie KR , Lieberherr D , Kim H , Zuccolo A , Wing RA , Nobuta K , Green PJ , Lu C , Meyers BC , Chaparro C , Piegu B , Panaud O , Echeverria M
Ref : Nucleic Acids Research , 36 :D1028 , 2008
Abstract : The Rice Annotation Project Database (RAP-DB) was created to provide the genome sequence assembly of the International Rice Genome Sequencing Project (IRGSP), manually curated annotation of the sequence, and other genomics information that could be useful for comprehensive understanding of the rice biology. Since the last publication of the RAP-DB, the IRGSP genome has been revised and reassembled. In addition, a large number of rice-expressed sequence tags have been released, and functional genomics resources have been produced worldwide. Thus, we have thoroughly updated our genome annotation by manual curation of all the functional descriptions of rice genes. The latest version of the RAP-DB contains a variety of annotation data as follows: clone positions, structures and functions of 31 439 genes validated by cDNAs, RNA genes detected by massively parallel signature sequencing (MPSS) technology and sequence similarity, flanking sequences of mutant lines, transposable elements, etc. Other annotation data such as Gnomon can be displayed along with those of RAP for comparison. We have also developed a new keyword search system to allow the user to access useful information. The RAP-DB is available at: http://rapdb.dna.affrc.go.jp/ and http://rapdb.lab.nig.ac.jp/.
ESTHER : Tanaka_2008_Nucleic.Acids.Res_36_D1028
PubMedSearch : Tanaka_2008_Nucleic.Acids.Res_36_D1028
PubMedID: 18089549
Gene_locus related to this paper: orysa-Q9FW17 , orysa-Q0JK71 , orysa-B9EWJ8 , orysa-Q5N7L1 , orysa-pir7a , orysa-q2qyj1 , orysj-q6yse8 , orysa-q6yzk1 , orysa-Q8S0U8 , orysa-q33aq0 , orysa-Q0J0A4 , orysi-a2z179 , orysi-a2zef2 , orysi-b8a7e6 , orysi-b8a7e7 , orysi-b8bfe5 , orysi-b8bhp9 , orysj-b9fi05 , orysj-b9fkb0 , orysj-cgep , orysj-q0djj0 , orysj-q0dud7 , orysj-q0jaf0 , orysj-q0jga1 , orysj-q5jl22 , orysj-q5jlw7 , orysj-q6h7q9 , orysj-q6yvk6 , orysj-q7f8x1 , orysj-q7xcx3 , orysj-q9fwm6 , orysj-q10j20 , orysj-q10ss2 , orysj-q69uw6 , orysj-q94d71 , orysj-q0iq98 , orysj-b9gbs4 , orysj-b9gbs1 , orysj-pla4 , orysj-pla1

Title : Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry - Suzuki_2007_Biosci.Biotechnol.Biochem_71_811
Author(s) : Suzuki Y , Miyazaki M , Ito E , Suzuki M , Yamashita T , Taira H , Suzuki A
Ref : Biosci Biotechnol Biochem , 71 :811 , 2007
Abstract : Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to (83)Asn was a bisecting complex type, Galbeta1-4GlcNAcbeta1-2Manalpha1-3(Galbeta1-4GlcNAcbeta1-2Manalpha1-6)(GlcNAcbeta 1-4)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc.
ESTHER : Suzuki_2007_Biosci.Biotechnol.Biochem_71_811
PubMedSearch : Suzuki_2007_Biosci.Biotechnol.Biochem_71_811
PubMedID: 17341822
Gene_locus related to this paper: felca-CAUXIN

Title : Effect of antimuscarinic drugs used for overactive bladder on learning in a rat passive avoidance response test - Suzuki_2007_Eur.J.Pharmacol_557_154
Author(s) : Suzuki M , Noguchi Y , Okutsu H , Ohtake A , Sasamata M
Ref : European Journal of Pharmacology , 557 :154 , 2007
Abstract : Antimuscarinic drugs are used for the treatment of overactive bladder. One adverse effect associated with their use, however, is cognitive impairment arising from their anticholinergic action. Here, we examined the effects of antimuscarinic drugs on learning using a passive avoidance task in rats. Drugs were intravenously administered 10 min before an acquisition trial, followed 24 h later by measurement of latency time in the passive avoidance task in a retention trial. Oxybutynin (0.1-1 mg/kg i.v.), propiverine (1-10 mg/kg i.v.) and scopolamine (0.1-1 mg/kg i.v.) impaired learning at doses of 0.3 mg/kg i.v. or more, 10 mg/kg i.v., and 0.3 mg/kg i.v. or more, respectively. Tolterodine (0.1-1 mg/kg i.v.) tended to impair these functions at a dose of 1 mg/kg i.v. In contrast, darifenacin (0.1-1 mg/kg i.v.) and solifenacin (0.3-3 mg/kg i.v.) showed no impairment of these functions. We also examined the effects of antimuscarinic drugs on learning enhanced by the cholinesterase inhibitor donepezil at a dose of 0.1 mg/kg i.v. in scopolamine-treated rats. Oxybutynin (0.1-1 mg/kg i.v.) impaired these donepezil-enhanced functions, whereas solifenacin (0.3-3 mg/kg i.v.) produced no significant impairment. These results suggest that antimuscarinic drugs such as darifenacin, solifenacin and tolterodine may have less effect on cognitive function in the treatment of patients with overactive bladder.
ESTHER : Suzuki_2007_Eur.J.Pharmacol_557_154
PubMedSearch : Suzuki_2007_Eur.J.Pharmacol_557_154
PubMedID: 17207478

Title : D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol alters cellular cholesterol homeostasis by modulating the endosome lipid domains - Makino_2006_Biochemistry_45_4530
Author(s) : Makino A , Ishii K , Murate M , Hayakawa T , Suzuki Y , Suzuki M , Ito K , Fujisawa T , Matsuo H , Ishitsuka R , Kobayashi T
Ref : Biochemistry , 45 :4530 , 2006
Abstract : D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP) is a frequently used inhibitor of glycosphingolipid biosynthesis. However, some interesting characteristics of D-PDMP cannot be explained by the inhibition of glycolipid synthesis alone. In the present study, we showed that d-PDMP inhibits the activation of lysosomal acid lipase by late endosome/lysosome specific lipid, bis(monoacylglycero)phosphate (also called as lysobisphosphatidic acid), through alteration of membrane structure of the lipid. When added to cultured fibroblasts, D-PDMP inhibits the degradation of low-density lipoprotein (LDL) and thus accumulates both cholesterol ester and free cholesterol in late endosomes/lysosomes. This accumulation results in the inhibition of LDL-derived cholesterol esterification and the decrease of cell surface cholesterol. We showed that D-PDMP alters cellular cholesterol homeostasis in a glycosphingolipid-independent manner using L-PDMP, a stereoisomer of D-PDMP, which does not inhibit glycosphingolipid synthesis, and mutant melanoma cell which is defective in glycolipid synthesis. Altering cholesterol homeostasis by D-PDMP explains the unique characteristics of sensitizing multidrug resistant cells by this drug.
ESTHER : Makino_2006_Biochemistry_45_4530
PubMedSearch : Makino_2006_Biochemistry_45_4530
PubMedID: 16584188

Title : Possible involvement of crosstalk cell-adhesion mechanism by endometrial CD26\/dipeptidyl peptidase IV and embryonal fibronectin in human blastocyst implantation - Shimomura_2006_Mol.Hum.Reprod_12_491
Author(s) : Shimomura Y , Ando H , Furugori K , Kajiyama H , Suzuki M , Iwase A , Mizutani S , Kikkawa F
Ref : Mol Hum Reprod , 12 :491 , 2006
Abstract : When human blastocysts hatch through the zona pellucida, gaining the ability to adhere to the endometrium, crosstalk between the embryo and the uterus may represent a successful outcome of their synchronized development and differentiation. CD26/dipeptidyl peptidase IV is known as a marker molecule of the implantation phase endometrium. To study the role of CD26 in implantation, 35 human hatched blastocysts were prepared by enzymatic treatment of expanded blastocysts that had been grown on schedule from frozen-thawed surplus embryos at the 2- or 4-cell stage. The blastocysts were placed on CD26-overexpressing or mock-transfected control monolayer cell cultures. The CD26-overexpression caused significantly higher blastocyst adhesion rate (53.3% versus 25.0%, P < 0.05) and significantly larger outgrowth area of trophectoderm (1.7-fold, P < 0.05). The second part of the present study was to show the expression of fibronectin, a CD26 ligand, in human preimplantation embryos, using the same donated resources. Fibronectin mRNA was detected by RT-PCR from the single hatched blastocyst (2/2) and from the single early blastocyst (3/6) but not from the single morula (0/5) samples. An indirect immunofluorescence technique verified the localization of fibronectin on the surface of the blastocyst. These results indicate that the adhesion mechanism by endometrial CD26 and embryonal fibronectin may be involved in human blastocyst implantation.
ESTHER : Shimomura_2006_Mol.Hum.Reprod_12_491
PubMedSearch : Shimomura_2006_Mol.Hum.Reprod_12_491
PubMedID: 16621928

Title : Antisense transcription in the mammalian transcriptome - Katayama_2005_Science_309_1564
Author(s) : Katayama S , Tomaru Y , Kasukawa T , Waki K , Nakanishi M , Nakamura M , Nishida H , Yap CC , Suzuki M , Kawai J , Suzuki H , Carninci P , Hayashizaki Y , Wells C , Frith M , Ravasi T , Pang KC , Hallinan J , Mattick J , Hume DA , Lipovich L , Batalov S , Engstrom PG , Mizuno Y , Faghihi MA , Sandelin A , Chalk AM , Mottagui-Tabar S , Liang Z , Lenhard B , Wahlestedt C
Ref : Science , 309 :1564 , 2005
Abstract : Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of the corresponding sense transcripts (RNA interference), as well as gene silencing at the chromatin level. Global transcriptome analysis provides evidence that a large proportion of the genome can produce transcripts from both strands, and that antisense transcripts commonly link neighboring "genes" in complex loci into chains of linked transcriptional units. Expression profiling reveals frequent concordant regulation of sense/antisense pairs. We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to control of transcriptional outputs in mammals.
ESTHER : Katayama_2005_Science_309_1564
PubMedSearch : Katayama_2005_Science_309_1564
PubMedID: 16141073
Gene_locus related to this paper: mouse-lipli , mouse-Ppgb , mouse-q3uuq7

Title : The transcriptional landscape of the mammalian genome - Carninci_2005_Science_309_1559
Author(s) : Carninci P , Kasukawa T , Katayama S , Gough J , Frith MC , Maeda N , Oyama R , Ravasi T , Lenhard B , Wells C , Kodzius R , Shimokawa K , Bajic VB , Brenner SE , Batalov S , Forrest AR , Zavolan M , Davis MJ , Wilming LG , Aidinis V , Allen JE , Ambesi-Impiombato A , Apweiler R , Aturaliya RN , Bailey TL , Bansal M , Baxter L , Beisel KW , Bersano T , Bono H , Chalk AM , Chiu KP , Choudhary V , Christoffels A , Clutterbuck DR , Crowe ML , Dalla E , Dalrymple BP , de Bono B , Della Gatta G , di Bernardo D , Down T , Engstrom P , Fagiolini M , Faulkner G , Fletcher CF , Fukushima T , Furuno M , Futaki S , Gariboldi M , Georgii-Hemming P , Gingeras TR , Gojobori T , Green RE , Gustincich S , Harbers M , Hayashi Y , Hensch TK , Hirokawa N , Hill D , Huminiecki L , Iacono M , Ikeo K , Iwama A , Ishikawa T , Jakt M , Kanapin A , Katoh M , Kawasawa Y , Kelso J , Kitamura H , Kitano H , Kollias G , Krishnan SP , Kruger A , Kummerfeld SK , Kurochkin IV , Lareau LF , Lazarevic D , Lipovich L , Liu J , Liuni S , McWilliam S , Madan Babu M , Madera M , Marchionni L , Matsuda H , Matsuzawa S , Miki H , Mignone F , Miyake S , Morris K , Mottagui-Tabar S , Mulder N , Nakano N , Nakauchi H , Ng P , Nilsson R , Nishiguchi S , Nishikawa S , Nori F , Ohara O , Okazaki Y , Orlando V , Pang KC , Pavan WJ , Pavesi G , Pesole G , Petrovsky N , Piazza S , Reed J , Reid JF , Ring BZ , Ringwald M , Rost B , Ruan Y , Salzberg SL , Sandelin A , Schneider C , Schonbach C , Sekiguchi K , Semple CA , Seno S , Sessa L , Sheng Y , Shibata Y , Shimada H , Shimada K , Silva D , Sinclair B , Sperling S , Stupka E , Sugiura K , Sultana R , Takenaka Y , Taki K , Tammoja K , Tan SL , Tang S , Taylor MS , Tegner J , Teichmann SA , Ueda HR , van Nimwegen E , Verardo R , Wei CL , Yagi K , Yamanishi H , Zabarovsky E , Zhu S , Zimmer A , Hide W , Bult C , Grimmond SM , Teasdale RD , Liu ET , Brusic V , Quackenbush J , Wahlestedt C , Mattick JS , Hume DA , Kai C , Sasaki D , Tomaru Y , Fukuda S , Kanamori-Katayama M , Suzuki M , Aoki J , Arakawa T , Iida J , Imamura K , Itoh M , Kato T , Kawaji H , Kawagashira N , Kawashima T , Kojima M , Kondo S , Konno H , Nakano K , Ninomiya N , Nishio T , Okada M , Plessy C , Shibata K , Shiraki T , Suzuki S , Tagami M , Waki K , Watahiki A , Okamura-Oho Y , Suzuki H , Kawai J , Hayashizaki Y
Ref : Science , 309 :1559 , 2005
Abstract : This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.
ESTHER : Carninci_2005_Science_309_1559
PubMedSearch : Carninci_2005_Science_309_1559
PubMedID: 16141072
Gene_locus related to this paper: mouse-abhd1 , mouse-abhd3 , mouse-abhd4 , mouse-acot4 , mouse-adcl4 , mouse-DGLB , mouse-ephx3 , mouse-Kansl3 , mouse-lipli , mouse-LIPN , mouse-Ppgb , mouse-q3uuq7 , mouse-srac1 , mouse-Tex30 , mouse-tmco4 , mouse-tmm53 , mouse-f172a

Title : The beneficial effect of donepezil on visual hallucinations in three patients with Parkinson's disease - Kurita_2003_J.Geriatr.Psychiatry.Neurol_16_184
Author(s) : Kurita A , Ochiai Y , Kono Y , Suzuki M , Inoue K
Ref : J Geriatr Psychiatry Neurol , 16 :184 , 2003
Abstract : Visual hallucinations (VHs) are common psychiatric symptoms in patients with long-standing Parkinson's disease (PD). Treatment with neuroleptics or withdrawal of anti-PD drugs may improve VHs but will worsen motor dysfunctions. The authors report on 3 patients with long-standing PD who were treated with the cholinesterase inhibitor donepezil for the treatment of VHs. Each received a daily dose of 5 mg of donepezil, after reducing or discontinuing anti-PD medications had failed to relieve the VHs. In 2 patients (patient 1, 2), donepezil decreased VHs without worsening motor dysfunctions. In addition, the cognitive status of patient 2 improved. In patient 3, donepezil also resolved VHs, but delusions developed during treatment. After discontinuing donepezil, delusions disappeared and VHs reappeared. Donepezil may ameliorate visual hallucinations in PD patients, but controlled, double-blind trials are necessary to further clarify the effect of this drug on VHs in PD.
ESTHER : Kurita_2003_J.Geriatr.Psychiatry.Neurol_16_184
PubMedSearch : Kurita_2003_J.Geriatr.Psychiatry.Neurol_16_184
PubMedID: 12967063

Title : Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution - Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168
Author(s) : Kawasaki K , Kondo H , Suzuki M , Ohgiya S , Tsuda S
Ref : Acta Crystallographica D Biol Crystallogr , 58 :1168 , 2002
Abstract : Bacillus subtilis extracellular lipase (BsL) has an exceptionally low molecular weight (19.4 kDa) for a member of the lipase family. A crystallographic study was performed on BsL in order to design and produce mutant BsL that will be more suitable for industrial uses based on analysis of the three-dimensional structure. Recently, the crystal structure of BsL has been determined at 1.5 A resolution [van Pouderoyen et al. (2001). J. Mol. Biol. 309, 215-226]. In the present study, a new crystal form of BsL which provides diffraction data to higher resolution was obtained and its structure was determined at 1.3 A using the MAD method. It was found that the active-site residue Ser77 has alternate side-chain conformations. The O(gamma) atom of the first conformer forms a hydrogen bond to the N(epsilon) atom of His155, a member of the catalytic triad. In contrast, the second conformer is constructed with a hydrogen bond to the side-chain atom of the adjacent His76. These two conformers presumably correspond to the active and inactive states, respectively. Similar alternate conformations in the catalytic serine residue have been observed in Fusarium solani cutinase determined at 1.0 A resolution and Penicillium purpurogenum acetylxylan esterase at 0.9 A resolution. In addition, a glycerol molecule, which was used as a cryoprotectant, is found to be located in the active site. On the basis of these results, a model for substrate binding in the reaction-intermediate state of BsL is proposed.
ESTHER : Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168
PubMedSearch : Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168
PubMedID: 12077437
Gene_locus related to this paper: bacsu-lip

Title : Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium - Kawashima_2000_Proc.Natl.Acad.Sci.U.S.A_97_14257
Author(s) : Kawashima T , Amano N , Koike H , Makino S , Higuchi S , Kawashima-Ohya Y , Watanabe K , Yamazaki M , Kanehori K , Kawamoto T , Nunoshiba T , Yamamoto Y , Aramaki H , Makino K , Suzuki M
Ref : Proc Natl Acad Sci U S A , 97 :14257 , 2000
Abstract : The complete genomic sequence of the archaeon Thermoplasma volcanium, possessing optimum growth temperature (OGT) of 60 degrees C, is reported. By systematically comparing this genomic sequence with the other known genomic sequences of archaea, all possessing higher OGT, a number of strong correlations have been identified between characteristics of genomic organization and the OGT. With increasing OGT, in the genomic DNA, frequency of clustering purines and pyrimidines into separate dinucleotides rises (e.g., by often forming AA and TT, whereas avoiding TA and AT). Proteins coded in a genome are divided into two distinct subpopulations possessing isoelectric points in different ranges (i.e., acidic and basic), and with increasing OGT the size of the basic subpopulation becomes larger. At the metabolic level, genes coding for enzymes mediating pathways for synthesizing some coenzymes, such as heme, start missing. These findings provide insights into the design of individual genomic components, as well as principles for coordinating changes in these designs for the adaptation to new environments.
ESTHER : Kawashima_2000_Proc.Natl.Acad.Sci.U.S.A_97_14257
PubMedSearch : Kawashima_2000_Proc.Natl.Acad.Sci.U.S.A_97_14257
PubMedID: 11121031
Gene_locus related to this paper: thevo-pip , thevo-TV0085 , thevo-TV0549 , thevo-TV0908 , thevo-TV1447 , thevo-TVG0742213 , thevo-TVG0965471 , thevo-TVG1026250 , thevo-TVG1221180 , thevo-TVG1473494

Title : Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana genomic region located around the 100 map unit of chromosome 1 - Kato_1999_Gene_239_309
Author(s) : Kato A , Suzuki M , Kuwahara A , Ooe H , Higano-Inaba K , Komeda Y
Ref : Gene , 239 :309 , 1999
Abstract : In order to analyze the organization of genes located at the 100 map unit of chromosome 1, we screened cDNAs hybridized with approximately 300kb contiguous DNA using four P1 clones and one YAC clone. A total of 40 kinds of cDNA were isolated, and their entire sequences were determined. A comparison with the GenBank/EMBL database indicated that three of the cDNAs have been found in Arabidopsis, and that similar sequences to 18 of the cDNAs had been detected in Arabidopsis or other organisms. cDNAs were aligned on a physical map of the contiguous DNA, and the transcriptional direction of each cDNA was determined. This contiguous DNA contains a large direct repeat, which contains five genes. In addition, identical or very similar sequences to two cDNAs are located in a narrow region. Thus, a total of 50 genes were identified, and the gene density was revealed to be approximately one gene every 6kb. In addition, cDNA sequencing revealed the existence of unusual transcripts. A sequence of seven cDNAs seemed to have no significant open reading frames. Furthermore, the existence of antisense RNA and the possibility of alternative splicing were also revealed.
ESTHER : Kato_1999_Gene_239_309
PubMedSearch : Kato_1999_Gene_239_309
PubMedID: 10548732
Gene_locus related to this paper: arath-ZW18

Title : Increased leukotriene A(4) hydrolase expression in the heart of angiotensin II-induced hypertensive rat - Ishizaka_1999_FEBS.Lett_463_155
Author(s) : Ishizaka N , Nakao A , Ohishi N , Suzuki M , Aizawa T , Taguchi J , Nagai R , Shimizu T , Ohno M
Ref : FEBS Letters , 463 :155 , 1999
Abstract : Leukotriene A(4) (LTA(4)) hydrolase is essential for the conversion of LTA(4) to LTB(4), an inflammatory lipid mediator. We investigated whether LTA(4) hydrolase was regulated in the heart by angiotensin II (ang II) infusion. Continuous ang II infusion via an osmotic minipump for up to 7 days upregulated mRNA and protein levels of LTA(4) hydrolase ( approximately 3.5-fold of control) in the heart in a pressor-dependent manner. Immunohistochemistry demonstrated intense LTA(4) hydrolase staining in the myofibroblast as well as migrated monocytes/macrophages. These data suggest that the cardiac LTA(4) hydrolase-LTB(4) system plays a positive role in the promotion of cardiac inflammation in hypertension.
ESTHER : Ishizaka_1999_FEBS.Lett_463_155
PubMedSearch : Ishizaka_1999_FEBS.Lett_463_155
PubMedID: 10601658

Title : Effect of KW-5092, novel gastroprokinetic agent, on the peristalsis in the isolated guinea pig ileum - Suzuki_1997_Jpn.J.Pharmacol_74_91
Author(s) : Suzuki M , Kishibayashi N , Yokoyama T , Karasawa A
Ref : Japanese Journal of Pharmacology , 74 :91 , 1997
Abstract : We examined the effect of KW-5092, a gastroprokinetic agent with acetylcholinesterase inhibitory and acetylcholine release facilitatory activities, on the peristalsis of isolated guinea pig ileum. KW-5092 (10(-9)-3 x 10(-6)M) increased the frequency of the peristaltic wave without changing its amplitude. Neostigmine increased the frequency at 10(-7) M, but domperidone (10(-8)-3 x 10(-6)M) had no effect on the peristalsis. The present results suggest that KW-5092 enhances the peristalsis via the inhibition of acetylcholinesterase, resulting in the intestinal propulsion.
ESTHER : Suzuki_1997_Jpn.J.Pharmacol_74_91
PubMedSearch : Suzuki_1997_Jpn.J.Pharmacol_74_91
PubMedID: 9195302

Title : Effect of YM796, a novel muscarinic agonist, on the impairment of passive avoidance response in senescence-accelerated mice - Suzuki_1995_Pharmacol.Biochem.Behav_51_623
Author(s) : Suzuki M , Yamaguchi T , Ozawa Y , Iwai A , Yamamoto M
Ref : Pharmacology, Biochemistry & Behavior , 51 :623 , 1995
Abstract : We compared the effects of YM796 [(-)-S-2,8-dimethyl-3-methylene-1-oxa-8- azaspiro[4,5]-decane L-tartrate monohydrate], a novel muscarinic agonist, on passive avoidance response with those of the cholinomimetics AF102B [(+/-)-cis-2-methylspiro-(1,3-oxathiolane-5,3')-quinuclidine hydrochloride] and NIK247 [9-amino-2,3,5,6,7,8-hexahydro1H-cyclopenta(b)- quinoline monohydrate hydrochloride] in senescence-accelerated mice. SAMP8@YAN (SAM-P/8, senescence-accelerated-prone substrain) showed an age-dependent shortening in the latency of step-through when compared with SAMR1/YAN (SAM-R/1, senescence-accelerated-resistant substrain). The shortened latency of step-through in SAMP8@YAN was prolonged by administration of YM796 (0.3 and 1 mg/kg, PO), AF102B (3 and 10 mg/kg PO), and NIK247 (30 mg/kg, PO) in a bell-shaped manner. In contrast, amitriptyline (10, 30, and 50 mg/kg, PO), with cholinolytic properties, had no effect on this shortened latency of step-through. These results suggest that YM796, AF102B, and NIK247 ameliorated the disturbance of learning behavior, presumably due to facilitation of the central cholinergic system in SAMP8@YAN mice and that SAMP8@YAN may be an appropriate age-dependent model of amnesia for evaluating pharmacological actions of drugs.
ESTHER : Suzuki_1995_Pharmacol.Biochem.Behav_51_623
PubMedSearch : Suzuki_1995_Pharmacol.Biochem.Behav_51_623
PubMedID: 7675834

Title : A case of VX poisoning and the difference from sarin [letter] -
Author(s) : Nozaki H , Aikawa N , Fujishima S , Suzuki M , Shinozawa Y , Hori S , Nogawa S
Ref : Lancet , 346 :698 , 1995
PubMedID: 765883

Title : Effects of (-)-S-2,8-dimethyl-3-methylene-1-oxa-8-azaspiro[4,5]decane L-tartrate monohydrate (YM796), a novel muscarinic agonist, on disturbance of passive avoidance learning behavior in drug-treated and senescence-accelerated mice - Suzuki_1995_J.Pharmacol.Exp.Ther_275_728
Author(s) : Suzuki M , Yamaguchi T , Ozawa Y , Ohyama M , Yamamoto M
Ref : Journal of Pharmacology & Experimental Therapeutics , 275 :728 , 1995
Abstract : Effects of YM796 (-)-S-2,8-dimethyl-3-methylene-1-oxa-8-azaspiro[4,5]decane L-tartrate monohydrate; a novel muscarinic agonist, were observed on disturbance of passive avoidance learning behavior in drug- (protein synthesis inhibitor and anticholinergic drugs) treated and senescence-accelerated mice in comparison with those of a muscarinic agonist (AF102B) and acetylcholinesterase inhibitors (E2020 (1-benzyl-4-[(5,6-dimethoxy-1-indanone-2-yl) methyl] piperidene hydrochloride), NIK247 [9-amino-2,3,5,6,7,8-hexahydro-1H-cyclopenta(b)-quinoline monohydrate hydrochloride], THA (9-amino-1,2,3,4-tetrahydroacridine) and physostigmine). All tested drugs administered before training significantly prolonged the shortened latency of step-through induced by the protein synthesis inhibitor cycloheximide (150 mg/kg s.c.). This shortened latency was also significantly prolonged when YM796 was administered immediately after training, but not when administered before the test trial. The ameliorating effect of YM796 on the impairment in learning behavior by cycloheximide was significantly suppressed by pirenzepine (0.1 micrograms/mouse i.c.v.). When administered before training, all test drugs prolonged the shortened latency of step-through induced by treatment with the anticholinergic drugs [scopolamine (1 mg/kg s.c.) and hemicholinium-3 (0.3 microgram/mouse i.c.v.)], suggesting that they ameliorated the impairment of learning behavior. This shortened latency in scopolamine-treated mice was also significantly prolonged by YM796, AF102B, E2020, NIK247 and physostigmine when administered immediately after training, but not when administered before the test trial. The pharmacological actions of YM796 administered immediately after training and before the test trial in hemicholinium-3-treated mice were similar to those in scopolamine-treated mice.
ESTHER : Suzuki_1995_J.Pharmacol.Exp.Ther_275_728
PubMedSearch : Suzuki_1995_J.Pharmacol.Exp.Ther_275_728
PubMedID: 7473160

Title : Pharmacological characterization of the nonpeptide angiotensin II receptor antagonist, U-97018 - Kushida_1995_J.Pharmacol.Exp.Ther_274_1042
Author(s) : Kushida H , Nomura S , Morita O , Harasawa Y , Suzuki M , Nakano M , Ozawa K , Kunihara M
Ref : Journal of Pharmacology & Experimental Therapeutics , 274 :1042 , 1995
Abstract : We examined the pharmacological properties of U-97018, a novel nonpeptide angiotensin II (AII) receptor antagonist, in various in vitro and in vivo studies. U-97018 selectively displaced 125I-AII specific binding in the membrane fraction derived from the rat mesenteric artery and adrenal cortex (AT1 subtype) with IC50 of 1.3 +/- 0.2 and 7.7 +/- 1.3 nM, respectively, without altering the AII binding of the rat adrenal medulla (AT2 subtype). In rat adrenal cortical cells, U-97018 inhibited 1 nM AII-induced aldosterone secretion with an IC50 of 0.48 nM; it shifted concentration-secretion response curve for AII to the right and inhibited the maximal response to AII, yielding a pKB of 9.8. Similarly, U-97018 showed insurmountable antagonism with a pKB of 10.6 against the AII-induced contraction in the isolated rabbit aorta. U-97018 had no direct effect on the activities of renin and angiotensin converting enzyme in vitro. In pithed rats, U-97018 inhibited the AII-induced pressor response with an ED50 of 0.28 mg/kg, i.v. without any partial agonistic activity. In anesthetized rats and dogs, intraduodenal administration of U-97018 at a dose of 1 mg/kg inhibited the AII-induced pressor response by about 60%. In spontaneously hypertensive rats, U-97018 at 10 mg/kg p.o. produced antihypertensive effects which lasted for 24 hr after administration. Thus, U-97018 is an orally active, insurmountable AII receptor antagonist without any agonistic activity.
ESTHER : Kushida_1995_J.Pharmacol.Exp.Ther_274_1042
PubMedSearch : Kushida_1995_J.Pharmacol.Exp.Ther_274_1042
PubMedID: 7562467

Title : Identification of a splicing mutation responsible for a human hereditary goiter with hypothyroidism. (Abstract) -
Author(s) : Cochaux P , Ieiri T , Targovnik H , Suzuki M , Shimoda SI , Perret J , Vassart G
Ref : American Journal of Human Genetics , 49 (suppl.) :131 , 1991
PubMedID:

Title : A 3' splice site mutation in the thyroglobulin gene responsible for congenital goiter with hypothyroidism - Ieiri_1991_J.Clin.Invest_88_1901
Author(s) : Ieiri T , Cochaux P , Targovnik HM , Suzuki M , Shimoda S , Perret J , Vassart G
Ref : J Clinical Investigation , 88 :1901 , 1991
Abstract : A case of congenital goiter with defective thyroglobulin synthesis has been studied in molecular terms. The patient is the fifth of a kindred of six, three of which have a goiter. The parents are first cousins. Segregation of thyroglobulin alleles in the family was studied by Southern blotting with a probe revealing a diallelic restriction fragment length polymorphism (RFLP). The results demonstrated that the three affected siblings were homozygous for the RFLP. Northern blotting analysis of the goiter RNA with a thyroglobulin probe suggested that thyroglobulin mRNA size was slightly reduced. Polymerase chain reaction amplification of the 8.5-kb thyroglobulin mRNA as overlapping cDNA fragments demonstrated that a 200-bp segment was missing from the 5' region of the goiter mRNA. Subcloning and sequencing of the cDNA fragments, and of the patient genomic DNA amplified from this region, revealed that exon 4 is missing from the major thyroglobulin transcript in the goiter, and that this aberrant splicing is due to a C to G transversion at position minus 3 in the acceptor splice site of intron 3. The presence in exon 4 of a putative donor tyrosine residue (Tyrosine nr 130) involved in thyroid hormone formation provides a coherent explanation to the hypothyroid status of the patient.
ESTHER : Ieiri_1991_J.Clin.Invest_88_1901
PubMedSearch : Ieiri_1991_J.Clin.Invest_88_1901
PubMedID: 1752952

Title : Lysosomal acid lipase deficiency in rats: lipid analyses and lipase activities in liver and spleen - Kuriyama_1990_J.Lipid.Res_31_1605
Author(s) : Kuriyama M , Yoshida H , Suzuki M , Fujiyama J , Igata A
Ref : J Lipid Res , 31 :1605 , 1990
Abstract : We report the biological characterization of an animal model of a genetic lipid storage disease analogous to human Wolman's disease. Affected rats accumulated cholesteryl esters (13.3-fold), free cholesterol (2.8-fold), and triglycerides (5.4-fold) in the liver, as well as cholesteryl esters (2.5-fold) and free cholesterol (1.33-fold) in the spleen. Triglycerides did not accumulate, and the levels actually decreased in the spleen. Analysis of the fatty acid composition of the cholesteryl esters and triglycerides showed high percentages of linoleic acid (18:2) and arachidonic acid (20:4) in both organs, especially in the liver. No accumulation of phospholipids, neutral glycosphingolipids, or gangliosides was found in the affected rats. Acid lipase activity for [14C]triolein, [14C]cholesteryl oleate, and 4-methyl-umbelliferyl oleate was deficient in both the liver and spleen of affected rats. Lipase activity at neutral pH was normal in both liver and spleen. Heterozygous rats showed intermediate utilization of these substrates in both organs at levels between those for affected rats and those for normal controls, although they did not accumulate any lipids. These data suggest that these rats represent an animal counterpart of Wolman's disease in humans.
ESTHER : Kuriyama_1990_J.Lipid.Res_31_1605
PubMedSearch : Kuriyama_1990_J.Lipid.Res_31_1605
PubMedID: 2246613
Gene_locus related to this paper: ratno-1llip

Title : Diagnostic and clinically important aspects of cyanobacterial (blue-green algae) toxicoses -
Author(s) : Beasley VR , Dahlem AM , Cook WO , Valentine WM , Lovell RA , Hooser SB , Harada K , Suzuki M , Carmichael WW
Ref : J Vet Diagn Invest , 1 :359 , 1989
PubMedID: 2518710