| Title : Gene Cloning and Characterization of a Poly(l-Lactic Acid) Depolymerase from Pseudomonas sp. Strain DS04-T - Wang_2011_J.Polym.Environ_19_827 |
| Author(s) : Wang ZY , Li F , Guo ZQ , Wang Y , Chen S |
| Ref : J. Polym. Environ , 19 :827 , 2011 |
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Abstract :
A gene encoding a poly(l-lactic acid) (PLA) depolymerase from Pseudomonas sp. strain DS04-T was cloned and overexpressed in Escherichia coli. The recombinant PLA depolymerase with a molecular weight of 19.2 kDa was purified to homogeneity. The optimum pH and temperature of the PLA depolymerase are 8.5 and 60 C, respectively. K+, Ca2+ and Ni2+ enhance the enzyme activity, while Na+, Zn2+, Mg2+, Cu2+, Fe2+, Mn2+ and Co2+ inhibit it. The inhibition of different chemicals on the PLA depolymerase activity were examined, in which EDTA was found to have a significantly inhibitory effect. The main degradation product of the depolymerase is identified as lactic acid monomer by mass spectrometric analysis. Physicochemical properties, substrate specificity and sequence analysis indicated that PME is a new type of PLA depolymerase |
| PubMedSearch : Wang_2011_J.Polym.Environ_19_827 |
| PubMedID: |
| Substrate | Polylactic-acid |
Wang ZY, Li F, Guo ZQ, Wang Y, Chen S (2011)
Gene Cloning and Characterization of a Poly(l-Lactic Acid) Depolymerase from Pseudomonas sp. Strain DS04-T
J. Polym. Environ
19 :827
Wang ZY, Li F, Guo ZQ, Wang Y, Chen S (2011)
J. Polym. Environ
19 :827