Werner_2021_Metab.Eng_67_250

Reference

Title : Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to beta-ketoadipic acid by Pseudomonas putida KT2440 - Werner_2021_Metab.Eng_67_250
Author(s) : Werner AZ , Clare R , Mand TD , Pardo I , Ramirez KJ , Haugen SJ , Bratti F , Dexter GN , Elmore JR , Huenemann JD , Peabody GLt , Johnson CW , Rorrer NA , Salvachua D , Guss AM , Beckham GT
Ref : Metab Eng , 67 :250 , 2021
Abstract : Poly(ethylene terephthalate) (PET) is the most abundantly consumed synthetic polyester and accordingly a major source of plastic waste. The development of chemocatalytic approaches for PET depolymerization to monomers offers new options for open-loop upcycling of PET, which can leverage biological transformations to higher-value products. To that end, here we perform four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2(II)A3(II)B(II)A1(II) from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) terephthalate (BHET) hydrolysis to TPA by expression of PETase and MHETase from Ideonella sakaiensis, and (iv) BHET conversion to a performance-advantaged bioproduct, beta-ketoadipic acid (betaKA) by deletion of pcaIJ. Using this strain, we demonstrate production of 15.1 g/L betaKA from BHET at 76% molar yield in bioreactors and conversion of catalytically depolymerized PET to betaKA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.
ESTHER : Werner_2021_Metab.Eng_67_250
PubMedSearch : Werner_2021_Metab.Eng_67_250
PubMedID: 34265401
Gene_locus related to this paper: idesa-mheth , idesa-peth

Related information

Gene_locus related to this paper: idesa-mheth , idesa-peth

Citations formats

Werner AZ, Clare R, Mand TD, Pardo I, Ramirez KJ, Haugen SJ, Bratti F, Dexter GN, Elmore JR, Huenemann JD, Peabody GLt, Johnson CW, Rorrer NA, Salvachua D, Guss AM, Beckham GT (2021)
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to beta-ketoadipic acid by Pseudomonas putida KT2440
Metab Eng 67 :250

Werner AZ, Clare R, Mand TD, Pardo I, Ramirez KJ, Haugen SJ, Bratti F, Dexter GN, Elmore JR, Huenemann JD, Peabody GLt, Johnson CW, Rorrer NA, Salvachua D, Guss AM, Beckham GT (2021)
Metab Eng 67 :250