de Vries_1997_Appl.Environ.Microbiol_63_4638

Reference

Title : The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides - de Vries_1997_Appl.Environ.Microbiol_63_4638
Author(s) : de Vries RP , Michelsen B , Poulsen CH , Kroon PA , van den Heuvel RH , Faulds CB , Williamson G , van den Hombergh JP , Visser J
Ref : Applied Environmental Microbiology , 63 :4638 , 1997
Abstract :

We report the cloning and characterization of a gene encoding a ferulic acid esterase, faeA, from Aspergillus niger and Aspergillus tubingensis. The A. niger and A. tubingensis genes have a high degree of sequence identity and contain one conserved intron. The gene product, FAEA, was overexpressed in wild-type A. tubingensis and a protease-deficient A. niger mutant. Overexpression of both genes in wild-type A. tubingensis and an A. niger protease-deficient mutant showed that the A. tubingensis gene product is more sensitive to degradation than the equivalent gene product from A. niger. FAEA from A. niger was identical to A. niger FAE-III (C. B. Faulds and G. Williamson, Microbiology 140:779-787, 1994), as assessed by molecular mass, pH and temperature optima, pI, N-terminal sequence, and activity on methyl ferulate. The faeA gene was induced by growth on wheat arabinoxylan and sugar beet pectin, and its gene product (FAEA) released ferulic acid from wheat arabinoxylan. The rate of release was enhanced by the presence of a xylanase. FAEA also hydrolyzed smaller amounts of ferulic acid from sugar beet pectin, but the rate was hardly affected by addition of an endo-pectin lyase.

PubMedSearch : de Vries_1997_Appl.Environ.Microbiol_63_4638
PubMedID: 9406381
Gene_locus related to this paper: aspni-FAEA , asptu-FAEA

Related information

Gene_locus aspni-FAEA    asptu-FAEA
Family Lipase_3

Citations formats

de Vries RP, Michelsen B, Poulsen CH, Kroon PA, van den Heuvel RH, Faulds CB, Williamson G, van den Hombergh JP, Visser J (1997)
The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides
Applied Environmental Microbiology 63 :4638

de Vries RP, Michelsen B, Poulsen CH, Kroon PA, van den Heuvel RH, Faulds CB, Williamson G, van den Hombergh JP, Visser J (1997)
Applied Environmental Microbiology 63 :4638