Paper Report for: Despotovic_2019_Protein.Eng.Des.Sel_32_169
Reference
Title: A mixture of three engineered phosphotriesterases enables rapid detoxification of the entire spectrum of known threat nerve agents Despotovic D, Aharon E, Dubovetskyi A, Leader H, Ashani Y, Tawfik DS Ref: Protein Engineering Des Sel, 32:169, 2019 : PubMed
Nerve agents are organophosphates (OPs) that potently inhibit acetylcholinesterase, and their enzymatic detoxification has been a long-standing goal. Nerve agents vary widely in size, charge, hydrophobicity and the cleavable ester bond. A single enzyme is therefore unlikely to efficiently hydrolyze all agents. Here, we describe a mixture of three previously developed variants of the bacterial phosphotriesterase (Bd-PTE) that are highly stable and nearly sequence identical. This mixture enables effective detoxification of a broad spectrum of known threat agents-GA (tabun), GB (sarin), GD (soman), GF (cyclosarin), VX and Russian-VX. The potential for dimer dissociation and exchange that could inactivate Bd-PTE has minimal impact, and the three enzyme variants are as active in a mixture as they are individually. To our knowledge, this engineered enzyme 'cocktail' comprises the first solution for enzymatic detoxification of the entire range of threat nerve agents.
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Citations formats
Despotovic D, Aharon E, Dubovetskyi A, Leader H, Ashani Y, Tawfik DS (2019) A mixture of three engineered phosphotriesterases enables rapid detoxification of the entire spectrum of known threat nerve agents Protein Engineering Des Sel32: 169-174
Despotovic D, Aharon E, Dubovetskyi A, Leader H, Ashani Y, Tawfik DS (2019) Protein Engineering Des Sel32: 169-174
