Gene Locus : human-ACHE
Mode of mutation : Site directed mutagenesis
Disease :
Summary : Catalysis Catalysis\/H-bond network\/active center modulation, reduced kapp for ACT and TB Ordentlich_1993_FEBS.Lett_334_215 Ordentlich_1995_5th.ChE.Meeting.Madras__221 Shafferman_1995_5th.ChE.Meeting.Madras__189 || Heat and pressure stability strengthened heat stability decreased pressure stability Clery-Barraud_2002_Eur.J.Biochem_269_4297
AAA Change :
Allelic Variant :
Risk Factor :
Inhibitor :
Structure :
Disease by interaction :
Interact Gene Locus :
Xenobiotic sensitivity :
Modification : H-bond network || Catalysis || Heat and pressure stability || Stability
Torpedo_number : 443
Kinetic Parameter : Tacrine_E450A_human-ACHE, HuperzineA_E450A_human-ACHE
News : No news
Comment :
p.E450A Glu450Ala (p.E481A Glu481Ala in primary sequence with 31 amino-acids signal peptide) Catalysis\/H-bond network\/active center modulation, reduced kapp for ACT and TB\; Heat and pressure stability: strengthened heat stability decreased pressure stability
| Title : Pressure and heat inactivation of recombinant human acetylcholinesterase. Importance of residue E202 for enzyme stability - Clery-Barraud_2002_Eur.J.Biochem_269_4297 |
| Author(s) : Clery-Barraud C , Ordentlich A , Grosfeld H , Shafferman A , Masson P |
| Ref : European Journal of Biochemistry , 269 :4297 , 2002 |
| Abstract : Clery-Barraud_2002_Eur.J.Biochem_269_4297 |
| ESTHER : Clery-Barraud_2002_Eur.J.Biochem_269_4297 |
| PubMedSearch : Clery-Barraud_2002_Eur.J.Biochem_269_4297 |
| PubMedID: 12199708 |
| Title : The 'aromatic patch' of three proximal residues in the human acetylcholinesterase active centre allows for versatile interaction modes with inhibitors - Ariel_1998_Biochem.J_335_95 |
| Author(s) : Ariel N , Ordentlich A , Barak D , Bino T , Velan B , Shafferman A |
| Ref : Biochemical Journal , 335 :95 , 1998 |
| Abstract : Ariel_1998_Biochem.J_335_95 |
| ESTHER : Ariel_1998_Biochem.J_335_95 |
| PubMedSearch : Ariel_1998_Biochem.J_335_95 |
| PubMedID: 9742217 |
| Title : Amino Acids Determining Specificity to OP-Agents and Facilitating the Aging Process in Human Acetylcholinesterase - |
| Author(s) : Ordentlich A , Kronman C , Stein D , Ariel N , Reuveny S , Marcus D , Segall Y , Barak D , Velan B , Shafferman A |
| Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :221 , 1995 |
| PubMedID: |
| Title : Molecular Aspects of Catalysis and of Allosteric Regulation of Aceytlcholinesterases - |
| Author(s) : Shafferman A , Ordentlich A , Barak D , Kronman C , Ariel N , Leitner M , Segall Y , Bromberg A , Reuveny S , Marcus D , Bino T , Lazar A , Cohen S , Velan B |
| Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :189 , 1995 |
| PubMedID: |
| Title : Engineering resistance to 'aging' of phosphylated human acetylcholinesterase. Role of hydrogen bond network in the active center - Ordentlich_1993_FEBS.Lett_334_215 |
| Author(s) : Ordentlich A , Kronman C , Barak D , Stein D , Ariel N , Marcus D , Velan B , Shafferman A |
| Ref : FEBS Letters , 334 :215 , 1993 |
| Abstract : Ordentlich_1993_FEBS.Lett_334_215 |
| ESTHER : Ordentlich_1993_FEBS.Lett_334_215 |
| PubMedSearch : Ordentlich_1993_FEBS.Lett_334_215 |
| PubMedID: 8224249 |