Amir A

General

Full name : Amir Adina

First name : Adina

Mail : Dept. of Biochemistry, Israel Inst. for Biol. Res., P.O.B. 19, 70450 Ness-Ziona

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Country : Israel

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Phone : (972) 8 381522

Fax : (972) 8 401094

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References (5)

Title : Prolonged impairment of corneal innervation after exposure to sulfur mustard and its relation to the development of delayed limbal stem cell deficiency - Kadar_2013_Cornea_32_e44
Author(s) : Kadar T , Dachir S , Cohen M , Gutman H , Cohen L , Brandeis R , Horwitz V , Amir A
Ref : Cornea , 32 :e44 , 2013
Abstract : PURPOSE: : Ocular injuries after exposure to the vesicant sulfur mustard (SM) are characterized by acute corneal erosions and inflammation of the anterior segment that may be followed by delayed limbal stem cell deficiency (LSCD), expressed clinically by corneal neovascularization and epithelial defects. The present study aimed to investigate the involvement of corneal nerves in the development of the delayed LSCD.
METHODS: : Rabbit eyes were exposed to SM vapor and observed clinically up to 1 month. Morphology and density of corneal nerves were studied in acetylcholinesterase-stained whole-mount corneas at different time points after exposure. Corneal calcitonin gene-related peptide (CGRP) was measured and the relation to clinical symptoms was tested.
RESULTS: : Degeneration of nerve terminals was observed a few hours after exposure simultaneously with the typical signs of SM ocular toxicity. Although corneal erosions healed within days, the nerves continued to disintegrate under a Wallerian degeneration pattern and their density declined significantly at 1 week in both central and peripheral corneal regions. Sprouting and regenerative nerve fibers were observed later in most of the corneas; however, healing was partial and often abnormal and was correlated with corneal edema. CGRP levels decreased at 24 hours and then increased significantly at 1 to 4 weeks, concomitant with the reinnervation process and development of the late injuries.
CONCLUSIONS: : The prolonged impairment of corneal nerves, together with chronic inflammation implied by edema, and abnormal increase in CGRP may contribute to a pathological environment for corneal epithelial stem cells, leading to their death and to the development of the SM-induced delayed LSCD.
ESTHER : Kadar_2013_Cornea_32_e44
PubMedSearch : Kadar_2013_Cornea_32_e44
PubMedID: 23132440

Title : A topical skin protectant against chemical warfare agents - Kadar_2003_Isr.Med.Assoc.J_5_717
Author(s) : Kadar T , Fishbine E , Meshulam J , Sahar R , Amir A , Barness I
Ref : Isr Med Assoc J , 5 :717 , 2003
Abstract : BACKGROUND: Sulfur mustard and VX are potent chemical warfare agents that penetrate rapidly through the skin, causing severe prolonged injuries and sometimes death. OBJECTIVES: To develop a topically applied pretreatment that will act as a barrier and prevent the absorption of these agents through the skin, reducing morbidity and saving life.
METHODS: Several formulations were developed and tested in preclinical animal studies in pigs. The protecting cream was applied as a single application (0.5-1 ml/100 cm2) prior to exposure (10 minutes to 12 hours) to sulfur mustard or VX. Assessment of sulfur mustard-induced skin damage was based on clinical and histologic evaluations. When tested against VX, clinical signs and blood cholinesterase activity were monitored. At the final stage of development, safety studies were conducted in animals and in human volunteers.
RESULTS: The formulation that gave the best results, coded IB1 (under patent application), provided significant protection against a 1 hour exposure to sulfur mustard (droplets or vapor). All the pigs pretreated with IB1 cream survived a 1-4 hour challenge of 2xLD50 VX and did not exhibit any overt clinical signs. Protection was exhibited even when the cream was applied 12 hours (single application) prior to exposure. IB1 was found to be non-irritating in animals and humans. No adverse effects were found in a Phase I clinical study in young healthy volunteers when the cream was applied to around 20% of the skin surface (results presented elsewhere).
CONCLUSIONS: IB1 cream has been shown to be a safe and effective topical skin protectant against the chemical warfare agents sulfur mustard and VX.
ESTHER : Kadar_2003_Isr.Med.Assoc.J_5_717
PubMedSearch : Kadar_2003_Isr.Med.Assoc.J_5_717
PubMedID: 14719467

Title : Autologous neuronal and muscle co-cultures: a model for neurotoxicology -
Author(s) : Shahar A , Bidder M , David Y , Amir A , Shainberg A
Ref : Prog Clin Biol Res , 253 :45 , 1987
PubMedID: 3432296

Title : Effects of phencyclidine and analog drugs on acetylcholine receptor of cultured muscle cells - Amir_1985_Biochem.Pharmacol_34_949
Author(s) : Amir A , Fuchs P , Gamliel A , Reis M , Shainberg A
Ref : Biochemical Pharmacology , 34 :949 , 1985
Abstract : Myotubes grown in culture provided a convenient experimental system for the study of the effects of phencyclidine (PCP) and analog drugs on both acetylcholine receptor (AChR) function and on its binding properties. The extent of PCP retention by these cells was studied on the same preparations. PCP, N-ethyl-l-phenylcyclohexylamine (PCE), PCP methiodide (PCPMeI), 1-[1-(3-aminophenyl)-cyclohexyl] piperidine (NH2PCP) and 1-[1-(2-thienyl)cyclohexyl] piperidine (TCP) were found to inhibit carbamylcholine (CbCh)-induced 22Na and 45Ca ion fluxes with 50% inhibition (I50) at 2-6 microM drug concentration. The I50 for CbCh-induced 42K+ efflux was 8-20 microM. Ketamine was less efficient with an I50 of 100 microM. Binding of [125I] alpha-bungarotoxin [( 125I]alpha-BGT) was not affected at drug concentrations that cause 100% inhibition of ion fluxes. Retention of [3H]PCP by the myotubes was a saturable process with half-maximal saturation at approximately 20 microM PCP. It was inhibited by PCP and several tertiary analogs, with and I50 of approximately 20 microM. PCPMeI was much less effective, with an I50 of 1 mM. PCPMeI was, however, as potent as PCP in its inhibition of the AChR function although the amount retained by the cells was 50-fold lower than that of PCP. These results are consistent with the theory that PCP and analog drugs affect AChR at a site other than the alpha-BGT binding site, possibly at the ionic channel of the nicotinic receptor.
ESTHER : Amir_1985_Biochem.Pharmacol_34_949
PubMedSearch : Amir_1985_Biochem.Pharmacol_34_949
PubMedID: 3985998

Title : N-allyl analogues of phencyclidine: chemical synthesis and pharmacological properties - Kalir_1984_J.Med.Chem_27_1267
Author(s) : Kalir A , Teomy S , Amir A , Fuchs P , Lee SA , Holsztynska EJ , Rocki W , Domino EF
Ref : Journal of Medicinal Chemistry , 27 :1267 , 1984
Abstract : Several N-allyl derivatives of 1-phenylcyclohexylamine (PCA) were prepared, and their pharmacology was briefly characterized. The mono- and diallyl derivatives had phencyclidine-like activities in mice but were less potent behaviorally than phencyclidine (PCP). None were PCP antagonists. In vitro these compounds were competitive inhibitors of butyrylcholinesterase (BChE) and protected against inhibition by DFP. In addition, these agents displaced tritiated N-methyl-4-piperidyl benzilate from mouse-brain homogenates and inhibited the effects of acetylcholine on isolated guinea pig ileum. None of these in vitro effects correlated with their PCP-like behavioral activity in vivo in mice.
ESTHER : Kalir_1984_J.Med.Chem_27_1267
PubMedSearch : Kalir_1984_J.Med.Chem_27_1267
PubMedID: 6481761