Friedrich A

References (4)

Title : Genome Sequence of the Yeast Cyberlindnera fabianii (Hansenula fabianii) - Freel_2014_Genome.Announc_2_e00638
Author(s) : Freel KC , Sarilar V , Neuveglise C , Devillers H , Friedrich A , Schacherer J
Ref : Genome Announc , 2 : , 2014
Abstract : The yeast Cyberlindnera fabianii is used in wastewater treatment, fermentation of alcoholic beverages, and has caused blood infections. To assist in the accurate identification of this species, and to determine the genetic basis for properties involved in fermentation and water treatment, we sequenced and annotated the genome of C. fabianii (YJS4271).
ESTHER : Freel_2014_Genome.Announc_2_e00638
PubMedSearch : Freel_2014_Genome.Announc_2_e00638
PubMedID: 25103752
Gene_locus related to this paper: cybfa-a0a061b0q2 , cybfa-a0a061ayy2 , cybfa-a0a061b6j0

Title : Pre- and postoperative nutritional deficiencies in obese patients undergoing laparoscopic sleeve gastrectomy - Damms-Machado_2012_Obes.Surg_22_881
Author(s) : Damms-Machado A , Friedrich A , Kramer KM , Stingel K , Meile T , Kuper MA , Konigsrainer A , Bischoff SC
Ref : Obes Surg , 22 :881 , 2012
Abstract : BACKGROUND: Laparoscopic sleeve gastrectomy (LSG) has been identified as an innovative surgical approach for the treatment of obesity and is increasingly applied worldwide. However, data on outcome of LSG regarding nutrient deficiencies, protein status, and body composition are scarce. METHODS: Obese subjects (54; f:m = 4:1) scheduled for LSG were included in this study. Micronutrient analysis, protein status assessment, and bioimpedance measures were performed before and 1, 3, 6, and 12 months after LSG. RESULTS: In 51% of the subjects, at least one micronutrient deficiency was found prior to surgery. Baseline concentrations were below normal for 25-OH vitamin D (27%), iron (29%), vitamin B6 (11%), vitamin B12 (9%), folate (6%), and potassium (7%). Frequencies of deficiencies for vitamin B12, folate, iron, and vitamin B6 tended to increase following LSG within the first year after intervention. Also, parameters of protein status (albumin, transferrin, cholinesterase, and total protein) decreased. After surgery, bioimpedance measures indicated a reduction of total body fat, but also of body cell mass. CONCLUSIONS: Preoperative micronutrient deficiencies were common in morbid obese individuals scheduled for LSG. LSG had a modest effect on micronutrient status by further reducing iron, vitamin B12, vitamin B6, and folate within the first year after intervention. Our data suggest that especially obese patients with preoperative deficits require control and supplementation of micronutrients and protein in the postoperative period.
ESTHER : Damms-Machado_2012_Obes.Surg_22_881
PubMedSearch : Damms-Machado_2012_Obes.Surg_22_881
PubMedID: 22403000

Title : Pichia sorbitophila, an Interspecies Yeast Hybrid, Reveals Early Steps of Genome Resolution After Polyploidization - Louis_2012_G3.(Bethesda)_2_299
Author(s) : Louis VL , Despons L , Friedrich A , Martin T , Durrens P , Casaregola S , Neuveglise C , Fairhead C , Marck C , Cruz JA , Straub ML , Kugler V , Sacerdot C , Uzunov Z , Thierry A , Weiss S , Bleykasten C , De Montigny J , Jacques N , Jung P , Lemaire M , Mallet S , Morel G , Richard GF , Sarkar A , Savel G , Schacherer J , Seret ML , Talla E , Samson G , Jubin C , Poulain J , Vacherie B , Barbe V , Pelletier E , Sherman DJ , Westhof E , Weissenbach J , Baret PV , Wincker P , Gaillardin C , Dujon B , Souciet JL
Ref : G3 (Bethesda) , 2 :299 , 2012
Abstract : Polyploidization is an important process in the evolution of eukaryotic genomes, but ensuing molecular mechanisms remain to be clarified. Autopolyploidization or whole-genome duplication events frequently are resolved in resulting lineages by the loss of single genes from most duplicated pairs, causing transient gene dosage imbalance and accelerating speciation through meiotic infertility. Allopolyploidization or formation of interspecies hybrids raises the problem of genetic incompatibility (Bateson-Dobzhansky-Muller effect) and may be resolved by the accumulation of mutational changes in resulting lineages. In this article, we show that an osmotolerant yeast species, Pichia sorbitophila, recently isolated in a concentrated sorbitol solution in industry, illustrates this last situation. Its genome is a mosaic of homologous and homeologous chromosomes, or parts thereof, that corresponds to a recently formed hybrid in the process of evolution. The respective parental contributions to this genome were characterized using existing variations in GC content. The genomic changes that occurred during the short period since hybrid formation were identified (e.g., loss of heterozygosity, unilateral loss of rDNA, reciprocal exchange) and distinguished from those undergone by the two parental genomes after separation from their common ancestor (i.e., NUMT (NUclear sequences of MiTochondrial origin) insertions, gene acquisitions, gene location movements, reciprocal translocation). We found that the physiological characteristics of this new yeast species are determined by specific but unequal contributions of its two parents, one of which could be identified as very closely related to an extant Pichia farinosa strain.
ESTHER : Louis_2012_G3.(Bethesda)_2_299
PubMedSearch : Louis_2012_G3.(Bethesda)_2_299
PubMedID: 22384408
Gene_locus related to this paper: picso-g8ycc9 , picso-g8yet0 , picso-g8yb96 , picso-g8ym39 , erecy-g8jrp5 , picso-g8y652

Title : Natural transformation in mesophilic and thermophilic bacteria: identification and characterization of novel, closely related competence genes in Acinetobacter sp. strain BD413 and Thermus thermophilus HB27 - Friedrich_2001_Appl.Environ.Microbiol_67_3140
Author(s) : Friedrich A , Hartsch T , Averhoff B
Ref : Applied Environmental Microbiology , 67 :3140 , 2001
Abstract : The mesophile Acinetobacter sp. strain BD413 and the extreme thermophile Thermus thermophilus HB27 display high frequencies of natural transformation. In this study we identified and characterized a novel competence gene in Acinetobacter sp. strain BD413, comA, whose product displays significant similarities to the competence proteins ComA and ComEC in Neisseria and Bacillus species. Transcription of comA correlated with growth phase-dependent transcriptional regulation of the recently identified pilin-like factors of the transformation machinery. This finding strongly suggests that comA is part of a competence regulon. Examination of the genome sequence of T. thermophilus HB27 led to detection of a comA/comEC-like open reading frame (ORF) which is flanked by an ORF whose product shows significant similarities to the Bacillus subtilis competence protein ComEA. To examine whether these two ORFs, designated comEC and comEA, are implicated in natural transformation of T. thermophilus HB27, both were disrupted by using a thermostable kanamycin resistance marker. Natural transformation in comEC mutants was reduced 1,000-fold, whereas in comEA mutants the natural transformation phenotype was completely eliminated. These results strongly suggest that both genes, comEC and comEA, are required for natural transformation in T. thermophilus HB27. Several transmembrane alpha-helices are predicted based on the amino acid sequences of ComA in Acinetobacter sp. strain BD413 and ComEC in T. thermophilus HB27, which suggests that ComA and ComEC are located in the inner membrane and function in DNA transport through the cytoplasmic membrane.
ESTHER : Friedrich_2001_Appl.Environ.Microbiol_67_3140
PubMedSearch : Friedrich_2001_Appl.Environ.Microbiol_67_3140
PubMedID: 11425734
Gene_locus related to this paper: acisp-Q9EYL8