Kyozuka J

References (14)

Title : What's New in Strigolactone Research? -
Author(s) : Yamaguchi S , Seto Y , Kyozuka J
Ref : Plant Cell Physiol , 64 :933 , 2023
PubMedID: 37655929

Title : Activation of Strigolactone Biosynthesis by the DWARF14-LIKE\/KARRIKIN-INSENSITIVE2 Pathway in Mycorrhizal Angiosperms, but Not in Arabidopsis, a Non-mycorrhizal Plant - Mashiguchi_2023_Plant.Cell.Physiol_64_1066
Author(s) : Mashiguchi K , Morita R , Tanaka K , Kodama K , Kameoka H , Kyozuka J , Seto Y , Yamaguchi S
Ref : Plant Cell Physiol , 64 :1066 , 2023
Abstract : Strigolactones (SLs) are a class of plant hormones that regulate many aspects of plant growth and development. SLs also improve symbiosis with arbuscular mycorrhizal fungi (AMF) in the rhizosphere. Recent studies have shown that the DWARF14-LIKE (D14L)/KARRIKIN-INSENSITIVE2 (KAI2) family, paralogs of the SL receptor D14, are required for AMF colonization in several flowering plants, including rice. In this study, we found that (-)-GR5, a 2'S-configured enantiomer of a synthetic SL analog (+)-GR5, significantly activated SL biosynthesis in rice roots via D14L. This result is consistent with a recent report, showing that the D14L pathway positively regulates SL biosynthesis in rice. In fact, the SL levels tended to be lower in the roots of the d14l mutant under both inorganic nutrient-deficient and -sufficient conditions. We also show that the increase in SL levels by (-)-GR5 was observed in other mycorrhizal plant species. In contrast, the KAI2 pathway did not upregulate the SL level and the expression of SL biosynthetic genes in Arabidopsis, a non-mycorrhizal plant. We also examined whether the KAI2 pathway enhances SL biosynthesis in the liverwort Marchantia paleacea, where SL functions as a rhizosphere signaling molecule for AMF. However, the SL level and SL biosynthetic genes were not positively regulated by the KAI2 pathway. These results imply that the activation of SL biosynthesis by the D14L/KAI2 pathway has been evolutionarily acquired after the divergence of bryophytes to efficiently promote symbiosis with AMF, although we cannot exclude the possibility that liverworts have specifically lost this regulatory system.
ESTHER : Mashiguchi_2023_Plant.Cell.Physiol_64_1066
PubMedSearch : Mashiguchi_2023_Plant.Cell.Physiol_64_1066
PubMedID: 37494415

Title : Unveiling the complexity of strigolactones: Exploring structural diversity, biosynthesis pathways and signaling mechanisms - Nomura_2023_J.Exp.Bot__
Author(s) : Nomura T , Seto Y , Kyozuka J
Ref : J Exp Bot , : , 2023
Abstract : Strigolactone (SL) is the collective name for compounds containing a butenolide as a part of their structure, first discovered as compounds that induce seed germination of root parasitic plants. They were later found to be rhizosphere signaling molecules that induce hyphal branching of arbuscular mycorrhizal (AM) fungi, and finally, they emerged as a class of plant hormones. SLs are found in root exudates, where they display a great variability in their chemical structure. Their structure varies among plant species, and multiple SLs can exist in one species. Over 30 SLs have been identified, yet the chemical structure of the SL that functions as an endogenous hormone and is found in the above-ground parts of plants remains unknown. We discuss our current knowledge of the synthetic pathways of diverse SLs and their regulation as well as recent progresses in identifying SLs as plant hormones. SL is perceived by the D14 receptor, an alpha/beta hydrolase which originated by gene duplication of KARRIKIN INSENSITIVE 2 (KAI2). D14 and KAI2 signaling pathways are partially overlapping paralogous pathways. Progress in understanding the signaling mechanisms mediated by two alpha/beta hydrolase receptors as well as remaining challenges in the field of SL research are reviewed.
ESTHER : Nomura_2023_J.Exp.Bot__
PubMedSearch : Nomura_2023_J.Exp.Bot__
PubMedID: 37877933

Title : KARRIKIN INSENSITIVE2 (KAI2)-dependent signaling pathway controls vegetative reproduction in Marchantia polymorpha - Komatsu_2022_Biorxiv__
Author(s) : Komatsu A , Kodama K , Mizuno Y , Fujibayashi M , Naramoto S , Kyozuka J
Ref : Biorxiv , : , 2022
Abstract : In vegetative reproduction of Marchantia polymorpha, propagules, called gemmae, are formed in gemma cups. Despite its significance for survival, control of gemma and gemma cup formation by environmental cues is not well understood. We show here that the number of gemmae formed in a gemma cup is a genetic trait. Gemma formation starts from the central region of the floor of the gemma cup, proceeds to the periphery, and terminates when the appropriate number of gemmae are initiated. The MpKARRIKIN INSENSITIVE2 (MpKAI2)-dependent signaling pathway promotes gemma cup formation and gemma initiation. The number of gemmae in a cup is controlled by modulating the ON/OFF switch of the KAI2-dependent signaling. Termination of the signaling results in the accumulation of MpSMXL, a suppressor protein. In the Mpsmxl mutants, gemma initiation continues, leading to the formation of a highly increased number of gemmae in a cup. Consistent with its function, the MpKAI2-dependent signaling pathway is active in gemma cups where gemmae initiate, as well as in the notch region of the mature gemma and midrib of the ventral side of the thallus. In this work, we also show that GEMMA CUP-ASSOCIATED MYB1 works downstream of this signaling pathway to promote gemma cup formation and gemma initiation. We also found that the availability of potassium affects gemma cup formation independently from the KAI2-dependent signaling pathway in M. polymorpha. We propose that the ancestral function of the KAI2-dependent signaling pathway may be to optimize vegetative reproduction by adapting to the environment.Competing Interest StatementThe authors have declared no competing interest.
ESTHER : Komatsu_2022_Biorxiv__
PubMedSearch : Komatsu_2022_Biorxiv__

Title : An ancestral function of strigolactones as symbiotic rhizosphere signals - Kodama_2022_Nat.Commun_13_3974
Author(s) : Kodama K , Rich MK , Yoda A , Shimazaki S , Xie X , Akiyama K , Mizuno Y , Komatsu A , Luo Y , Suzuki H , Kameoka H , Libourel C , Keller J , Sakakibara K , Nishiyama T , Nakagawa T , Mashiguchi K , Uchida K , Yoneyama K , Tanaka Y , Yamaguchi S , Shimamura M , Delaux PM , Nomura T , Kyozuka J
Ref : Nat Commun , 13 :3974 , 2022
Abstract : In flowering plants, strigolactones (SLs) have dual functions as hormones that regulate growth and development, and as rhizosphere signaling molecules that induce symbiosis with arbuscular mycorrhizal (AM) fungi. Here, we report the identification of bryosymbiol (BSB), an SL from the bryophyte Marchantia paleacea. BSB is also found in vascular plants, indicating its origin in the common ancestor of land plants. BSB synthesis is enhanced at AM symbiosis permissive conditions and BSB deficient mutants are impaired in AM symbiosis. In contrast, the absence of BSB synthesis has little effect on the growth and gene expression. We show that the introduction of the SL receptor of Arabidopsis renders M. paleacea cells BSB-responsive. These results suggest that BSB is not perceived by M. paleacea cells due to the lack of cognate SL receptors. We propose that SLs originated as AM symbiosis-inducing rhizosphere signaling molecules and were later recruited as plant hormone.
ESTHER : Kodama_2022_Nat.Commun_13_3974
PubMedSearch : Kodama_2022_Nat.Commun_13_3974
PubMedID: 35803942

Title : Desmethyl butenolides are optimal ligands for karrikin receptor proteins - Yao_2021_New.Phytol__
Author(s) : Yao J , Scaffidi A , Meng Y , Melville KT , Komatsu A , Khosla A , Nelson DC , Kyozuka J , Flematti GR , Waters MT
Ref : New Phytol , : , 2021
Abstract : Strigolactones and karrikins are butenolide molecules that regulate plant growth. They are perceived via the alpha/beta-hydrolase DWARF14 (D14) and its homologue KARRIKIN INSENSITIVE2 (KAI2), respectively. Plant-derived strigolactones have a butenolide ring with a methyl group that is essential for bioactivity. By contrast, karrikins are abiotic in origin, and the butenolide methyl group is non-essential. KAI2 is probably a receptor for an endogenous butenolide, but the identity of this compound remains unknown. Here we characterise the specificity of KAI2 towards differing butenolide ligands using genetic and biochemical approaches. We find that KAI2 proteins from multiple species are most sensitive to desmethyl butenolides that lack a methyl group. Desmethyl-GR24 and desmethyl-CN-debranone are active via KAI2 but not D14. They are more potent KAI2 agonists than their methyl-substituted reference compounds both in vitro and in plants. The preference of KAI2 for desmethyl butenolides is conserved in Selaginella moellendorffii and Marchantia polymorpha, suggesting that it is an ancient trait in land plant evolution. Our findings provide insight into the mechanistic basis for differential ligand perception by KAI2 and D14, and support the view that the endogenous substrates for KAI2 and D14 have distinct chemical structures and biosynthetic origins.
ESTHER : Yao_2021_New.Phytol__
PubMedSearch : Yao_2021_New.Phytol__
PubMedID: 33474738
Gene_locus related to this paper: arath-AtD14 , arath-KAI2.D14L

Title : Major components in the KARRIKIN INSENSITIVE2-ligand signaling pathway are conserved in the liverwort, Marchantia polymorpha - Mizuno_2020_Biorxiv__
Author(s) : Mizuno Y , Komatsu A , Shimazaki S , Xie X , Ishizaki K , Naramoto S , Kyozuka J
Ref : Biorxiv , 11.17.387852 : , 2020
Abstract : KARRIKIN INSENSITIVE2 (KAI2) was first identified in Arabidopsis thaliana as a receptor of karrikin, a smoke-derived germination stimulant. KAI2 is also considered a receptor of an unidentified endogenous molecule called the KAI2-ligand (KL). Upon KAI2 activation, signals are transmitted through degradation of D53/SMXL proteins via ubiquitination by a Skp-Cullin-F-box (SCF) E3 ubiquitin ligase complex. All components in the KL signaling pathway exist in the liverwort Marchantia polymorpha, namely MpKAI2A and MpKAI2B, MpMAX2 encoding the F-box protein, and MpSMXL, indicating that the signaling pathway became functional in the common ancestor of bryophytes and seed plants. Genetic analysis using knock-out mutants of these KL signaling genes, produced using the CRISPR system, indicated that MpKAI2A, MpMAX2 and MpSMXL act in the same genetic pathway and control early gemma growth. Introduction of MpSMXLd53, in which a domain required for degradation is mutated, into wild-type plants caused phenotypes resembling those of the Mpkai2a and Mpmax2 mutants. In addition, Citrine fluorescence was detected in tobacco cells transiently transformed with the 35S:MpSMXL-Citrine gene construct and treated with MG132, a proteasome inhibitor. On the other hand, introduction of 35S:MpSMXLd53-Citrine conferred Citrine fluorescence without MG132 treatment. These findings imply that MpSMXL is subjected to degradation, and that degradation of MpSMXL is crucial for KL signaling in M. polymorpha. We also showed that MpSMXL is negatively regulated by KL signaling. Taken together, this study demonstrates that basic mechanisms in the KL signaling pathway are conserved in M. polymorpha.
ESTHER : Mizuno_2020_Biorxiv__
PubMedSearch : Mizuno_2020_Biorxiv__

Title : Strigolactone perception and deactivation by a hydrolase receptor DWARF14 - Seto_2019_Nat.Commun_10_191
Author(s) : Seto Y , Yasui R , Kameoka H , Tamiru M , Cao M , Terauchi R , Sakurada A , Hirano R , Kisugi T , Hanada A , Umehara M , Seo E , Akiyama K , Burke J , Takeda-Kamiya N , Li W , Hirano Y , Hakoshima T , Mashiguchi K , Noel JP , Kyozuka J , Yamaguchi S
Ref : Nat Commun , 10 :191 , 2019
Abstract : The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an alpha/beta-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14(D218A) catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.
ESTHER : Seto_2019_Nat.Commun_10_191
PubMedSearch : Seto_2019_Nat.Commun_10_191
PubMedID: 30643123
Gene_locus related to this paper: arath-AtD14

Title : Phloem Transport of the Receptor DWARF14 Protein Is Required for Full Function of Strigolactones - Kameoka_2016_Plant.Physiol_172_1844
Author(s) : Kameoka H , Dun EA , Lopez-Obando M , Brewer PB , de Saint Germain A , Rameau C , Beveridge CA , Kyozuka J
Ref : Plant Physiol , 172 :1844 , 2016
Abstract : The cell-to-cell transport of signaling molecules is essential for multicellular organisms to coordinate the action of their cells. Recent studies identified DWARF14 (D14) as a receptor of strigolactones (SLs), molecules that act as plant hormones and inhibit shoot branching. Here, we demonstrate that RAMOSUS3, a pea ortholog of D14, works as a graft-transmissible signal to suppress shoot branching. In addition, we show that D14 protein is contained in phloem sap and transported through the phloem to axillary buds in rice. SLs are not required for the transport of D14 protein. Disruption of D14 transport weakens the suppression of axillary bud outgrowth of rice. Taken together, we conclude that the D14 protein works as an intercellular signaling molecule to fine-tune SL function. Our findings provide evidence that the intercellular transport of a receptor can regulate the action of plant hormones.
ESTHER : Kameoka_2016_Plant.Physiol_172_1844
PubMedSearch : Kameoka_2016_Plant.Physiol_172_1844
PubMedID: 27670819

Title : Structures of D14 and D14L in the strigolactone and karrikin signaling pathways - Kagiyama_2013_Genes.Cells_18_147
Author(s) : Kagiyama M , Hirano Y , Mori T , Kim SY , Kyozuka J , Seto Y , Yamaguchi S , Hakoshima T
Ref : Genes Cells , 18 :147 , 2013
Abstract : Strigolactones (SLs) are plant hormones that inhibit shoot branching. DWARF14 (D14) inhibits rice tillering and is an SL receptor candidate in the branching inhibition pathway, whereas the close homologue DWARF14-LIKE (D14L) participates in the signaling pathway of karrikins (KARs), which are derived from burnt vegetation as smoke stimulants of seed germination. We provide the first evidence for direct binding of the bioactive SL analogue GR24 to D14. Isothermal titration calorimetry measurements show a D14-GR24 binding affinity in the sub-micromolar range. Similarly, bioactive KAR1 directly binds D14L in the micromolar range. The crystal structure of rice D14 shows a compact alpha-/beta-fold hydrolase domain forming a deep ligand-binding pocket capable of accommodating GR24. Insertion of four alpha-helices between beta6 strand and alphaD helix forms the helical cap of the pocket, although the pocket is open to the solvent. The pocket contains the conserved catalytic triad Ser-His-Asp aligned with the oxyanion hole, suggesting hydrolase activity. Although these structural characteristics are conserved in D14L, the D14L pocket is smaller than that of D14. The KAR-insensitive mutation kai2-1 is located at the prominent long beta6-alphaD1 loop, which is characteristic in D14 and D14L, but not in related alpha-/beta-fold hydrolases.
ESTHER : Kagiyama_2013_Genes.Cells_18_147
PubMedSearch : Kagiyama_2013_Genes.Cells_18_147
PubMedID: 23301669
Gene_locus related to this paper: arath-KAI2.D14L , orysj-Q10QA5

Title : New branching inhibitors and their potential as strigolactone mimics in rice - Fukui_2011_Bioorg.Med.Chem.Lett_21_4905
Author(s) : Fukui K , Ito S , Ueno K , Yamaguchi S , Kyozuka J , Asami T
Ref : Bioorganic & Medicinal Chemistry Lett , 21 :4905 , 2011
Abstract : Strigolactones (SLs) are rhizosphere communication chemicals. Recent studies of highly branched mutants revealed that SL or its metabolites work as a phytohormone to inhibit shoot branching. When SLs are exogenously applied to the rice d10-1 mutant that has a highly branched phenotype caused by a defect in the SL biosynthesis gene (CCD8), they inhibit tiller bud outgrowth (branching in rice) of the mutant. We focused our attention on the SL function as a phytohormone and tried to find new chemicals mimicking the hormonal action of SL by screening chemicals that inhibit branching of rice d10-1 mutant. Fortunately, we found 5-(4-chlorophenoxy)-3-methylfuran-2(5H)-one (3a) as a new chemical possessing SL-like activity against the rice d10-1 mutant. Then, we prepared several derivatives of 3a (3b-3k) to examine their ability to inhibit shoot branching of rice d10-1. These derivatives were synthesized by a one-pot coupling reaction between phenols and halo butenolide to give 5-phenoxy 3-methylfuran-2(5H)-one (3) derivatives, which possess a common substructure with SLs. Some of the derivatives showed SL-like activity more potently than GR24, a typical SL derivative, in a rice assay. As SLs also show activity by inducing seed germination of root parasitic plants, the induction activity of these derivatives was also evaluated. Here we report the structure-activity relationships of these compounds.
ESTHER : Fukui_2011_Bioorg.Med.Chem.Lett_21_4905
PubMedSearch : Fukui_2011_Bioorg.Med.Chem.Lett_21_4905
PubMedID: 21741836

Title : A new lead chemical for strigolactone biosynthesis inhibitors - Ito_2010_Plant.Cell.Physiol_51_1143
Author(s) : Ito S , Kitahata N , Umehara M , Hanada A , Kato A , Ueno K , Mashiguchi K , Kyozuka J , Yoneyama K , Yamaguchi S , Asami T
Ref : Plant Cell Physiol , 51 :1143 , 2010
Abstract : Several triazole-containing chemicals have previously been shown to act as efficient inhibitors of cytochrome P450 monooxygenases. To discover a strigolactone biosynthesis inhibitor, we screened a chemical library of triazole derivatives to find chemicals that induce tiller bud outgrowth of rice seedlings. We discovered a triazole-type chemical, TIS13 [2,2-dimethyl-7-phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol], which induced outgrowth of second tiller buds of wild-type seedlings, as observed for non-treated strigolactone-deficient d10 mutant seedlings. TIS13 treatment reduced strigolactone levels in both roots and root exudates in a concentration-dependent manner. Co-application of GR24, a synthetic strigolactone, with TIS13 canceled the TIS13-induced tiller bud outgrowth. Taken together, these results indicate that TIS13 inhibits strigolactone biosynthesis in rice seedlings. We propose that TIS13 is a new lead compound for the development of specific strigolactone biosynthesis inhibitors.
ESTHER : Ito_2010_Plant.Cell.Physiol_51_1143
PubMedSearch : Ito_2010_Plant.Cell.Physiol_51_1143
PubMedID: 20522488

Title : d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers - Arite_2009_Plant.Cell.Physiol_50_1416
Author(s) : Arite T , Umehara M , Ishikawa S , Hanada A , Maekawa M , Yamaguchi S , Kyozuka J
Ref : Plant Cell Physiol , 50 :1416 , 2009
Abstract : Recent studies using highly branched mutants of pea, Arabidopsis and rice have demonstrated that strigolactones, a group of terpenoid lactones, act as a new hormone class, or its biosynthetic precursors, in inhibiting shoot branching. Here, we provide evidence that DWARF14 (D14) inhibits rice tillering and may act as a new compo-nent of the strigolactone-dependent branching inhibition pathway. The d14 mutant exhibits increased shoot branch-ing with reduced plant height like the previously characterized strigolactone-deficient and -insensitive mutants d10 and d3, respectively. The d10-1 d14-1 double mutant is phenotypically indistinguishable from the d10-1 and d14-1 single mutants, consistent with the idea that D10 and D14 function in the same pathway. However, unlike with d10, the d14 branching phenotype could not be rescued by exogenous strigolactones. In addition, the d14 mutant contained a higher level of 2'-epi-5-deoxystrigol than the wild type. Positional cloning revealed that D14 encodes a protein of the alpha/beta-fold hydrolase superfamily, some members of which play a role in metabolism or signaling of plant hormones. We propose that D14 functions downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form.
ESTHER : Arite_2009_Plant.Cell.Physiol_50_1416
PubMedSearch : Arite_2009_Plant.Cell.Physiol_50_1416
PubMedID: 19542179
Gene_locus related to this paper: orysj-Q10QA5

Title : Inhibition of shoot branching by new terpenoid plant hormones - Umehara_2008_Nature_455_195
Author(s) : Umehara M , Hanada A , Yoshida S , Akiyama K , Arite T , Takeda-Kamiya N , Magome H , Kamiya Y , Shirasu K , Yoneyama K , Kyozuka J , Yamaguchi S
Ref : Nature , 455 :195 , 2008
Abstract : Shoot branching is a major determinant of plant architecture and is highly regulated by endogenous and environmental cues. Two classes of hormones, auxin and cytokinin, have long been known to have an important involvement in controlling shoot branching. Previous studies using a series of mutants with enhanced shoot branching suggested the existence of a third class of hormone(s) that is derived from carotenoids, but its chemical identity has been unknown. Here we show that levels of strigolactones, a group of terpenoid lactones, are significantly reduced in some of the branching mutants. Furthermore, application of strigolactones inhibits shoot branching in these mutants. Strigolactones were previously found in root exudates acting as communication chemicals with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Thus, we propose that strigolactones act as a new hormone class or their biosynthetic precursors in regulating above-ground plant architecture, and also have a function in underground communication with other neighbouring organisms.
ESTHER : Umehara_2008_Nature_455_195
PubMedSearch : Umehara_2008_Nature_455_195
PubMedID: 18690207