arath-KAI2.D14L

Arabidopsis thaliana (Mouse-ear cress) At4g37470 KAI2 AtKAI2 HTL hyposensitive to light

Comment

orthologue to rice OsDLK1 paralogue to AtD14 DWARF14 || KARRIKIN INSENSITIVE2 (KAI2) karrikin receptor. Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Lacks detectable hydrolase activity against karrikin. Karrikin binding induces a conformational change. Mutations in KAI2, encoding the proposed karrikin receptor, result in hypersensitivity to water deprivation. HYPOSENSITIVE TO LIGHT or HTL. Mutant displayed a long hypocotyl phenotype under red, far-red, and blue light

Relationship

Family : RsbQ-like

Block : X

Position in NCBI Life Tree : Arabidopsis thaliana

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Viridiplantae N E > Streptophyta N E > Streptophytina N E > Embryophyta N E > Tracheophyta N E > Euphyllophyta N E > Spermatophyta N E > Magnoliophyta N E > Mesangiospermae N E > eudicotyledons N E > Gunneridae N E > Pentapetalae N E > rosids N E > malvids N E > Brassicales N E > Brassicaceae N E > Camelineae N E > Arabidopsis N E > Arabidopsis thaliana N E

Molecular evidence

No mutation

5YZ7 - Crystal structure of DWARF14 (KAI2) karrikin receptor D14-D-ringopened 7'-carba-4BD complex 5Z9G - Crystal structure of DWARF14 (KAI2) karrikin receptor 5Z9H - Crystal structure of KAI2_ply2(A219V) DWARF14 (KAI2) karrikin receptor 4JYP - crystal Structure of KAI2 Apo form 4JYM - crystal Structure of KAI2 in complex with KAR1 3W06 - Crystal structure of Arabidopsis DWARF14-LIKE (D14L) AtD14L 8U33 - Crystal structure of KAI2 S95C mutant 8U2L - Crystal structure of KAI2 S95C L48I mutant 4HRY - The structure of Arabidopsis thaliana KAI2 4HTA - The structure of the karrikin insensitive (KAI2) protein in Arabidopsis thaliana 4HRX - Crystal structure of KAI2 4IH1 - Crystal structure of Karrikin Insensitive 2 (KAI2) from Arabidopsis thaliana

No kinetic

No disease

5-Deoxystrigol 7-carba-4BD Contalactone GR24 Orobanchol Xilatone-Red Yoshimulactone-Green desmethyl-Yoshimulactone-Green

4-Br-Debranone 4-CN-Debranone 4-CN-desmethyl-Debranone 4-Cl-Debranone 7-carba-4BD 7-carba-4BD-ring-opened Germinone-A dMgerminone

Database

AL035601 AL161591

Q9SZU7

Sequence

Peptide

MGVVEEAHNV KVIGSGEATI VLGHGFGTDQ SVWKHLVPHL VDDYRVVLYD NMGAGTTNPD YFDFDRYSNL EGYSFDLIAI LEDLKIESCI FVGHSVSAMI GVLASLNRPD LFSKIVMISA SPRYVNDVDY QGGFEQEDLN QLFEAIRSNY KAWCLGFAPL AVGGDMDSIA VQEFSRTLFN MRPDIALSVG QTIFQSDMRQ ILPFVTVPCH ILQSVKDLAV PVVVSEYLHA NLGCESVVEV IPSDGHLPQL SSPDSVIPVI LRHIRNDIAM

References (16)

Title : KAI2 regulates seedling development by mediating light-induced remodelling of auxin transport - Hamon-Josse_2022_New.Phytol_235_126

Author(s) : Hamon-Josse M , Villaecija-Aguilar JA , Ljung K , Leyser O , Gutjahr C , Bennett T

Ref : New Phytol , 235 :126 , 2022

PubMedID: 35313031

Gene_locus related to this paper: arath-KAI2.D14L

Title : KAI2 promotes Arabidopsis root hair elongation at low external phosphate by controlling local accumulation of AUX1 and PIN2 - Villaecija-Aguilar_2021_Curr.Biol__

Author(s) : Villaecija-Aguilar JA , Korosy C , Maisch L , Hamon-Josse M , Petrich A , Magosch S , Chapman P , Bennett T , Gutjahr C

Ref : Current Biology , : , 2021

PubMedID: 34758285

Gene_locus related to this paper: arath-KAI2.D14L

Title : Desmethyl butenolides are optimal ligands for karrikin receptor proteins - Yao_2021_New.Phytol__

Author(s) : Yao J , Scaffidi A , Meng Y , Melville KT , Komatsu A , Khosla A , Nelson DC , Kyozuka J , Flematti GR , Waters MT

Ref : New Phytol , : , 2021

PubMedID: 33474738

Gene_locus related to this paper: arath-AtD14 , arath-KAI2.D14L

Title : Three mutations repurpose a plant karrikin receptor to a strigolactone receptor - Arellano-Saab_2021_Proc.Natl.Acad.Sci.U.S.A_118_

Author(s) : Arellano-Saab A , Bunsick M , Al Galib H , Zhao W , Schuetz S , Bradley JM , Xu Z , Adityani C , Subha A , McKay H , de Saint Germain A , Boyer FD , McErlean CSP , Toh S , McCourt P , Stogios PJ , Lumba S

Ref : Proc Natl Acad Sci U S A , 118 : , 2021

PubMedID: 34301902

Gene_locus related to this paper: arath-AtD14 , arath-KAI2.D14L

Title : Synthetic agonist of HTL\/KAI2 shows potent stimulating activity for Arabidopsis seed germination - Fukui_2019_Bioorg.Med.Chem.Lett_29_2487

Author(s) : Fukui K , Arai K , Kasahara H , Asami T , Hayashi KI

Ref : Bioorganic & Medicinal Chemistry Lett , 29 :2487 , 2019

PubMedID: 31345632

Gene_locus related to this paper: arath-KAI2.D14L

Title : An allelic series at the KARRIKIN INSENSITIVE 2 locus of Arabidopsis thaliana decouples ligand hydrolysis and receptor degradation from downstream signalling - Yao_2018_Plant.J_96_75

Author(s) : Yao J , Mashiguchi K , Scaffidi A , Akatsu T , Melville KT , Morita R , Morimoto Y , Smith SM , Seto Y , Flematti GR , Yamaguchi S , Waters MT

Ref : Plant J , 96 :75 , 2018

PubMedID: 29982999

Gene_locus related to this paper: arath-KAI2.D14L

Title : A missense allele of KARRIKIN-INSENSITIVE2 impairs ligand-binding and downstream signaling in Arabidopsis thaliana - Lee_2018_J.Exp.Bot_69_3609

Author(s) : Lee I , Kim K , Lee S , Hwang E , Shin K , Kim D , Choi J , Choi H , Cha JS , Kim H , Lee RA , Jeong S , Kim J , Kim Y , Nam HG , Park SK , Cho HS , Soh MS

Ref : J Exp Bot , 69 :3609 , 2018

PubMedID: 29722815

Gene_locus related to this paper: arath-KAI2.D14L

Title : Rationally Designed Strigolactone Analogs as Antagonists of the D14 Receptor - Takeuchi_2018_Plant.Cell.Physiol_59_1545

Author(s) : Takeuchi J , Jiang K , Hirabayashi K , Imamura Y , Wu Y , Xu Y , Miyakawa T , Nakamura H , Tanokura M , Asami T

Ref : Plant Cell Physiol , 59 :1545 , 2018

PubMedID: 29727000

Gene_locus related to this paper: arath-AtD14 , arath-KAI2.D14L

Title : The karrikin receptor KAI2 promotes drought resistance in Arabidopsis thaliana - Li_2017_PLoS.Genet_13_e1007076

Author(s) : Li W , Nguyen KH , Chu HD , Ha CV , Watanabe Y , Osakabe Y , Leyva-Gonzalez MA , Sato M , Toyooka K , Voges L , Tanaka M , Mostofa MG , Seki M , Seo M , Yamaguchi S , Nelson DC , Tian C , Herrera-Estrella L , Tran LP

Ref : PLoS Genet , 13 :e1007076 , 2017

PubMedID: 29131815

Gene_locus related to this paper: arath-KAI2.D14L

Title : Substrate-Induced Degradation of the alpha\/beta-Fold Hydrolase KARRIKIN INSENSITIVE2 Requires a Functional Catalytic Triad but Is Independent of MAX2 -

Author(s) : Waters MT , Scaffidi A , Flematti GR , Smith SM

Ref : Mol Plant , 8 :814 , 2015

PubMedID: 25698586

Gene_locus related to this paper: arath-KAI2.D14L

Title : The Structure of the Karrikin-Insensitive Protein (KAI2) in Arabidopsis thaliana. - Bythell-Douglas_2013_PLoS.One_8_e54758

Author(s) : Bythell-Douglas R , Waters MT , Scaffidi A , Flematti GR , Smith SM , Bond CS

Ref : PLoS ONE , 8 :e54758 , 2013

PubMedID: 23349965

Gene_locus related to this paper: arath-KAI2.D14L

Title : KAI2- and MAX2-Mediated Responses to Karrikins and Strigolactones Are Largely Independent of HY5 in Arabidopsis Seedlings - Waters_2013_Mol.Plant_6_63

Author(s) : Waters MT , Smith SM

Ref : Mol Plant , 6 :63 , 2013

PubMedID: 23142794

Gene_locus related to this paper: arath-KAI2.D14L

Title : Structures of D14 and D14L in the strigolactone and karrikin signaling pathways - Kagiyama_2013_Genes.Cells_18_147

Author(s) : Kagiyama M , Hirano Y , Mori T , Kim SY , Kyozuka J , Seto Y , Yamaguchi S , Hakoshima T

Ref : Genes Cells , 18 :147 , 2013

PubMedID: 23301669

Gene_locus related to this paper: arath-KAI2.D14L , orysj-Q10QA5

Title : Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis. - Waters_2012_Development_139_1285

Author(s) : Waters MT , Nelson DC , Scaffidi A , Flematti GR , Sun YK , Dixon KW , Smith SM

Ref : Development , 139 :1285 , 2012

PubMedID: 22357928

Gene_locus related to this paper: arath-AtD14 , arath-KAI2.D14L , orysj-q10j20 , orysj-Q10QA5

Title : HYPOSENSITIVE TO LIGHT, an alpha\/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation - Sun_2011_Mol.Plant_4_116

Author(s) : Sun XD , Ni M

Ref : Mol Plant , 4 :116 , 2011

PubMedID: 20864454

Gene_locus related to this paper: arath-KAI2.D14L

Title : Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. - Bevan_1998_Nature_391_485

Author(s) : Bevan M , Bancroft I , Bent E , Love K , Goodman H , Dean C , Bergkamp R , Dirkse W , van Staveren M , Stiekema W , Drost L , Ridley P , Hudson SA , Patel K , Murphy G , Piffanelli P , Wedler H , Wedler E , Wambutt R , Weitzenegger T , Pohl TM , Terryn N , Gielen J , Villarroel R , De Clerck R , van Montagu M , Lecharny A , Auborg S , Gy I , Kreis M , Lao N , Kavanagh T , Hempel S , Kotter P , Entian KD , Rieger M , Schaeffer M , Funk B , Mueller-Auer S , Silvey M , James R , Montfort A , Pons A , Puigdomenech P , Douka A , Voukelatou E , Milioni D , Hatzopoulos P , Piravandi E , Obermaier B , Hilbert H , Duesterhoft A , Moores T , Jones JDG , Eneva T , Palme K , Benes V , Rechman S , Ansorge W , Cooke R , Berger C , Delseny M , Voet M , Volckaert G , Mewes HW , Klosterman S , Schueller C , Chalwatzis N

Ref : Nature , 391 :485 , 1998

PubMedID: 9461215

Gene_locus related to this paper: arath-a4vcl8 , arath-AT4G00500 , arath-AT4g09900 , arath-AT4g12830 , arath-AT4G14290 , arath-AT4G15100 , arath-AT4G16070 , arath-AT4G16690 , arath-AT4G17150 , arath-AT4G17470 , arath-AT4G17480 , arath-AT4G17483 , arath-At4g18550 , arath-SOBR1 , arath-SOBRL , arath-AT4G24380 , arath-AT4G25770 , arath-AT4g30610 , arath-AT4G31020 , arath-AT4G36195 , arath-AT4G37150 , arath-SCP29 , arath-At3g54240 , arath-KAI2.D14L

arath-KAI2.D14L

Comment

Relationship

Molecular evidence

Mutation (0)

Structure (12)

Kinetic (0)

Disease (0)

Substrate (8)

Inhibitor (8)

Database

Genbank (2)

Interpro (1)

Pfam (1)

UniProt (1)

PIRSF (1)

SUPERFAM (1)

Sequence

Peptide

References (16)

1. KAI2 regulates seedling development by mediating light-induced remodelling of auxin transport - Hamon-Josse_2022_New.Phytol_235_126

2. KAI2 promotes Arabidopsis root hair elongation at low external phosphate by controlling local accumulation of AUX1 and PIN2 - Villaecija-Aguilar_2021_Curr.Biol__

3. Desmethyl butenolides are optimal ligands for karrikin receptor proteins - Yao_2021_New.Phytol__

4. Three mutations repurpose a plant karrikin receptor to a strigolactone receptor - Arellano-Saab_2021_Proc.Natl.Acad.Sci.U.S.A_118_

5. Synthetic agonist of HTL\/KAI2 shows potent stimulating activity for Arabidopsis seed germination - Fukui_2019_Bioorg.Med.Chem.Lett_29_2487

6. An allelic series at the KARRIKIN INSENSITIVE 2 locus of Arabidopsis thaliana decouples ligand hydrolysis and receptor degradation from downstream signalling - Yao_2018_Plant.J_96_75

7. A missense allele of KARRIKIN-INSENSITIVE2 impairs ligand-binding and downstream signaling in Arabidopsis thaliana - Lee_2018_J.Exp.Bot_69_3609

8. Rationally Designed Strigolactone Analogs as Antagonists of the D14 Receptor - Takeuchi_2018_Plant.Cell.Physiol_59_1545

9. The karrikin receptor KAI2 promotes drought resistance in Arabidopsis thaliana - Li_2017_PLoS.Genet_13_e1007076

10. Substrate-Induced Degradation of the alpha\/beta-Fold Hydrolase KARRIKIN INSENSITIVE2 Requires a Functional Catalytic Triad but Is Independent of MAX2 -

11. The Structure of the Karrikin-Insensitive Protein (KAI2) in Arabidopsis thaliana. - Bythell-Douglas_2013_PLoS.One_8_e54758

12. KAI2- and MAX2-Mediated Responses to Karrikins and Strigolactones Are Largely Independent of HY5 in Arabidopsis Seedlings - Waters_2013_Mol.Plant_6_63

13. Structures of D14 and D14L in the strigolactone and karrikin signaling pathways - Kagiyama_2013_Genes.Cells_18_147

14. Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis. - Waters_2012_Development_139_1285

15. HYPOSENSITIVE TO LIGHT, an alpha\/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation - Sun_2011_Mol.Plant_4_116

16. Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. - Bevan_1998_Nature_391_485