Mori T

References (18)

Title : In vitro evaluation of novel SN-38 prodrug activated by alpha-rhamnosidase of exogenous enzyme - Nii_2024_Anal.Sci__
Author(s) : Nii T , Hijii S , Kaneko R , Tanito K , Yamanaka K , Kishimura A , Mori T , Katayama Y
Ref : Anal Sci , : , 2024
Abstract : This study introduces the alpha-rhamnose (Rham)-conjugated prodrug of SN-38 (Rham-SN-38) as a promising alternative to irinotecan. alpha-rhamnosidase, responsible for SN-38 release from Rham-SN-38, does not express in human cells, minimizing individual variability and side effects. The injection of the alpha-rhamnosidase into the tumor tissues makes it possible, for the first time, to activate the Rham-SN-38. Furthermore, alpha-rhamnosidase demonstrates significantly higher activity than carboxylesterase, the specific enzyme activating irinotecan. SN-38 release mediated by alpha-rhamnosidase completes within 2 h, with a k(cat)/K(m) value approximately 5.0 x 10(4)-fold higher than that of irinotecan. The 50% inhibition concentration (IC(50)) of Rham-SN-38 against three types of cancer cells and one normal cell exceeds 4.5 x 10(3) nM. The addition of alpha-rhamnosidase significantly increases cytotoxicity, with IC(50) comparable to free SN-38. The QIC(50), an index reflecting the difference in cytotoxicity with and without alpha-rhamnosidase, exceeds approximately 1.0 x 10(2)-fold. Rham-SN-38, synthesized in this study, demonstrates significant potential as a prodrug for cancer therapy.
ESTHER : Nii_2024_Anal.Sci__
PubMedSearch : Nii_2024_Anal.Sci__
PubMedID: 38748393

Title : Asymmetric Open-Closed Dimer Mechanism of Polyhydroxyalkanoate Synthase PhaC - Chek_2020_iScience_23_101084
Author(s) : Chek MF , Kim SY , Mori T , Tan HT , Sudesh K , Hakoshima T
Ref : iScience , 23 :101084 , 2020
Abstract : Biodegradable polyester polyhydroxyalkanoate (PHA) is a promising bioplastic material for industrial use as a replacement for petroleum-based plastics. PHA synthase PhaC forms an active dimer to polymerize acyl moieties from the substrate acyl-coenzyme A (CoA) into PHA polymers. Here we present the crystal structure of the catalytic domain of PhaC from Chromobacterium sp. USM2, bound to CoA. The structure reveals an asymmetric dimer, in which one protomer adopts an open conformation bound to CoA, whereas the other adopts a closed conformation in a CoA-free form. The open conformation is stabilized by the asymmetric dimerization and enables PhaC to accommodate CoA and also to create the product egress path. The bound CoA molecule has its beta-mercaptoethanolamine moiety extended into the active site with the terminal SH group close to active center Cys291, enabling formation of the reaction intermediate by acylation of Cys291.
ESTHER : Chek_2020_iScience_23_101084
PubMedSearch : Chek_2020_iScience_23_101084
PubMedID: 32388399
Gene_locus related to this paper: 9neis-e1apk1

Title : Structure function and engineering of multifunctional non-heme iron dependent oxygenases in fungal meroterpenoid biosynthesis - Nakashima_2018_Nat.Commun_9_104
Author(s) : Nakashima Y , Mori T , Nakamura H , Awakawa T , Hoshino S , Senda M , Senda T , Abe I
Ref : Nat Commun , 9 :104 , 2018
Abstract : Non-heme iron and alpha-ketoglutarate (alphaKG) oxygenases catalyze remarkably diverse reactions using a single ferrous ion cofactor. A major challenge in studying this versatile family of enzymes is to understand their structure-function relationship. AusE from Aspergillus nidulans and PrhA from Penicillium brasilianum are two highly homologous Fe(II)/alphaKG oxygenases in fungal meroterpenoid biosynthetic pathways that use preaustinoid A1 as a common substrate to catalyze divergent rearrangement reactions to form the spiro-lactone in austinol and cycloheptadiene moiety in paraherquonin, respectively. Herein, we report the comparative structural study of AusE and PrhA, which led to the identification of three key active site residues that control their reactivity. Structure-guided mutagenesis of these residues results in successful interconversion of AusE and PrhA functions as well as generation of the PrhA double and triple mutants with expanded catalytic repertoire. Manipulation of the multifunctional Fe(II)/alphaKG oxygenases thus provides an excellent platform for the future development of biocatalysts.
ESTHER : Nakashima_2018_Nat.Commun_9_104
PubMedSearch : Nakashima_2018_Nat.Commun_9_104
PubMedID: 29317628
Gene_locus related to this paper: penbi-prhl

Title : Improvement of Visuo-spatial Function Assessed by Raven's Colored Progressive Matrices in Dementia with Lewy Bodies by Donepezil Treatment - Yoshino_2017_Clin.Psychopharmacol.Neurosci_15_243
Author(s) : Yoshino Y , Mori T , Yoshida T , Toyota Y , Shimizu H , Iga JI , Nishitani S , Ueno SI
Ref : Clin Psychopharmacol Neurosci , 15 :243 , 2017
Abstract : Objective: Donepezil is used to improve cognitive impairment of dementia with Lewy bodies (DLB). Visuo-spatial dysfunction is a well-known symptom of DLB. Non-verbal Raven's Colored Progressive Matrices (RCPM) were used to assess both visual perception and reasoning ability in DLB subjects treated with donepezil. Methods: Twenty-one DLB patients (mean age, 78.7+/-4.5 years) were enrolled. RCPM assessment was performed at the time of starting donepezil and within one year after starting donepezil. Results: There were significant improvements of RCPM in the total scores between one year donepezil treatment (p=0.013), in both Set A score (p=0.002) and Set AB score (p=0.015), but trend in the Set B score (p=0.083). Conclusion: Donepezil is useful for improving visuo-spatial impairment in DLB, but not for problem-solving impairment.
ESTHER : Yoshino_2017_Clin.Psychopharmacol.Neurosci_15_243
PubMedSearch : Yoshino_2017_Clin.Psychopharmacol.Neurosci_15_243
PubMedID: 28783933

Title : Molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis - Mori_2017_Nat.Chem.Biol_13_1066
Author(s) : Mori T , Iwabuchi T , Hoshino S , Wang H , Matsuda Y , Abe I
Ref : Nat Chemical Biology , 13 :1066 , 2017
Abstract : Trt14 from Aspergillus terreus is involved in unusual skeletal reconstruction during the biosynthesis of the fungal meroterpenoid terretonin. Detailed in vitro characterization revealed that this novel multifunctional enzyme catalyzes not only the D-ring expansion via intramolecular methoxy rearrangement, but also the hydrolysis of the expanded D-ring. The X-ray crystal structures of Trt14, in complex with substrate or product, and two Trt14 homologs, AusH and PrhC from Aspergillus nidulans and Penicillium brasilianum, respectively, indicated similar overall structures to those of the NTF2-like superfamily of enzymes, despite lacking sequence and functional similarities. Moreover, we gained structural insight into the mechanism of the Trt14-catalyzed ring reconstruction from the in-crystal enzyme reaction and site-directed mutagenesis to show that this reaction involves sequential ester bond cleavage and formation. Structural comparison of Trt14 and its homologs suggests that the enzymes in this new superfamily employ similar acid-base chemistry to diversify the molecular architecture of fungal meroterpenoids.
ESTHER : Mori_2017_Nat.Chem.Biol_13_1066
PubMedSearch : Mori_2017_Nat.Chem.Biol_13_1066
PubMedID: 28759016
Gene_locus related to this paper: asptn-trt4 , penbi-prhl

Title : Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plastics - Chek_2017_Sci.Rep_7_5312
Author(s) : Chek MF , Kim SY , Mori T , Arsad H , Samian MR , Sudesh K , Hakoshima T
Ref : Sci Rep , 7 :5312 , 2017
Abstract : Polyhydroxyalkanoate (PHA) is a promising candidate for use as an alternative bioplastic to replace petroleum-based plastics. Our understanding of PHA synthase PhaC is poor due to the paucity of available three-dimensional structural information. Here we present a high-resolution crystal structure of the catalytic domain of PhaC from Chromobacterium sp. USM2, PhaC Cs -CAT. The structure shows that PhaC Cs -CAT forms an alpha/beta hydrolase fold comprising alpha/beta core and CAP subdomains. The active site containing Cys291, Asp447 and His477 is located at the bottom of the cavity, which is filled with water molecules and is covered by the partly disordered CAP subdomain. We designated our structure as the closed form, which is distinct from the recently reported catalytic domain from Cupriavidus necator (PhaC Cn -CAT). Structural comparison showed PhaC Cn -CAT adopting a partially open form maintaining a narrow substrate access channel to the active site, but no product egress. PhaC Cs -CAT forms a face-to-face dimer mediated by the CAP subdomains. This arrangement of the dimer is also distinct from that of the PhaC Cn -CAT dimer. These findings suggest that the CAP subdomain should undergo a conformational change during catalytic activity that involves rearrangement of the dimer to facilitate substrate entry and product formation and egress from the active site.
ESTHER : Chek_2017_Sci.Rep_7_5312
PubMedSearch : Chek_2017_Sci.Rep_7_5312
PubMedID: 28706283
Gene_locus related to this paper: 9neis-e1apk1

Title : Discovery of Key Dioxygenases that Diverged the Paraherquonin and Acetoxydehydroaustin Pathways in Penicillium brasilianum - Matsuda_2016_J.Am.Chem.Soc_138_12671
Author(s) : Matsuda Y , Iwabuchi T , Fujimoto T , Awakawa T , Nakashima Y , Mori T , Zhang H , Hayashi F , Abe I
Ref : Journal of the American Chemical Society , 138 :12671 , 2016
Abstract : Paraherquonin (1), a fungal meroterpenoid produced by Penicillium brasilianum NBRC 6234, possesses a unique, highly congested hexacyclic molecular architecture. Here we identified the biosynthetic gene cluster of 1 (the prh cluster) and elucidated the pathway up to berkeleydione (2), which serves as the key intermediate for the biosynthesis of 1 as well as many other meroterpenoids. Interestingly, the nonheme iron and alpha-ketoglutarate-dependent dioxygenase PrhA constructs the cycloheptadiene moiety to afford 2 from preaustinoid A1 (6), probably via the homoallyl-homoallyl radical rearrangement. Additionally, another fungal strain, P. brasilianum MG11, which produces acetoxydehydroaustin instead of 1, was found to have a gene cluster nearly identical to the prh cluster. The dioxygenase encoded by the cluster shares 92% sequence identity with PrhA, and also accepts 6 but produces preaustinoid A3 (17) with a spiro-lactone system, generating a diverging point for the two different meroterpenoid pathways in the same species.
ESTHER : Matsuda_2016_J.Am.Chem.Soc_138_12671
PubMedSearch : Matsuda_2016_J.Am.Chem.Soc_138_12671
PubMedID: 27602587
Gene_locus related to this paper: penbi-ausa , penbi-prhl

Title : Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes - Hosokawa_2015_Biosens.Bioelectron_67_379
Author(s) : Hosokawa M , Hoshino Y , Nishikawa Y , Hirose T , Yoon DH , Mori T , Sekiguchi T , Shoji S , Takeyama H
Ref : Biosensors & Bioelectronics , 67 :379 , 2015
Abstract : This paper proposes a high-throughput, function-based screening approach of a metagenomic library for isolating novel microbial enzymes by droplet-based microfluidics. We used gel microdroplets (GMDs) dispersed in oil as picoliter-volume reaction vessels for lipolytic enzyme by encapsulating cells in individual GMDs. Using this approach, we monitored the growth of individual cells encapsulated in GMDs and assessed the enzyme reaction activities at the level of an individual GMD. We then applied this method to screen lipolytic enzyme genes from the metagenomic library constructed from soil collected from a quercus serrate forest of Mount Tsukuba, Ibaraki, Japan. In the workflow presented in this study, metagenomic library clones were encapsulated in 100-pL GMDs with a fluorogenic reporter substrate. A total of 67,000 metagenomic library clones can be screened in only 24 h with reduced consumption of reagents (i.e., <10 muL). As a result, we identified a novel lipolytic enzyme, EstT1, belonging to the EstD2 family of esterases and containing a putative signal peptide, which facilitates enzyme export and catalyzation of substrates in the periplasm. Our study demonstrates the potential of microfluidic GMDs as an efficient tool for metagenomic library screening of industrially relevant enzymes with the potential of significantly reducing the cost and time factors involved in successful practical application of microbial enzymes.
ESTHER : Hosokawa_2015_Biosens.Bioelectron_67_379
PubMedSearch : Hosokawa_2015_Biosens.Bioelectron_67_379
PubMedID: 25194237
Gene_locus related to this paper: 9bact-a0a077l869

Title : An environmental bacterial taxon with a large and distinct metabolic repertoire - Wilson_2014_Nature_506_58
Author(s) : Wilson MC , Mori T , Ruckert C , Uria AR , Helf MJ , Takada K , Gernert C , Steffens UA , Heycke N , Schmitt S , Rinke C , Helfrich EJ , Brachmann AO , Gurgui C , Wakimoto T , Kracht M , Crusemann M , Hentschel U , Abe I , Matsunaga S , Kalinowski J , Takeyama H , Piel J
Ref : Nature , 506 :58 , 2014
Abstract : Cultivated bacteria such as actinomycetes are a highly useful source of biomedically important natural products. However, such 'talented' producers represent only a minute fraction of the entire, mostly uncultivated, prokaryotic diversity. The uncultured majority is generally perceived as a large, untapped resource of new drug candidates, but so far it is unknown whether taxa containing talented bacteria indeed exist. Here we report the single-cell- and metagenomics-based discovery of such producers. Two phylotypes of the candidate genus 'Entotheonella' with genomes of greater than 9 megabases and multiple, distinct biosynthetic gene clusters co-inhabit the chemically and microbially rich marine sponge Theonella swinhoei. Almost all bioactive polyketides and peptides known from this animal were attributed to a single phylotype. 'Entotheonella' spp. are widely distributed in sponges and belong to an environmental taxon proposed here as candidate phylum 'Tectomicrobia'. The pronounced bioactivities and chemical uniqueness of 'Entotheonella' compounds provide significant opportunities for ecological studies and drug discovery.
ESTHER : Wilson_2014_Nature_506_58
PubMedSearch : Wilson_2014_Nature_506_58
PubMedID: 24476823
Gene_locus related to this paper: 9delt-w4lci7 , 9delt-w4lkq9 , 9delt-w4lj58 , 9delt-w4l7a4 , 9delt-w4m617

Title : Complete biosynthetic pathway of anditomin: nature's sophisticated synthetic route to a complex fungal meroterpenoid - Matsuda_2014_J.Am.Chem.Soc_136_15326
Author(s) : Matsuda Y , Wakimoto T , Mori T , Awakawa T , Abe I
Ref : Journal of the American Chemical Society , 136 :15326 , 2014
Abstract : Anditomin and its precursors, andilesins, are fungal meroterpenoids isolated from Aspergillus variecolor and have unique, highly oxygenated chemical structures with a complex bridged-ring system. Previous isotope-feeding studies revealed their origins as 3,5-dimethylorsellinic acid and farnesyl pyrophosphate and suggested the possible involvement of a Diels-Alder reaction to afford the congested bicyclo[2.2.2]octane core structure of andilesins. Here we report the first identification of the biosynthetic gene cluster of anditomin and the determination of the complete biosynthetic pathway by characterizing the functions of 12 dedicated enzymes. The anditomin pathway actually does not employ a Diels-Alder reaction, but involves the nonheme iron-dependent dioxygenase AndA to synthesize the bridged-ring by an unprecedented skeletal reconstruction. Another dioxygenase, AndF, is also responsible for the structural complexification, generating the end product anditomin by an oxidative rearrangement.
ESTHER : Matsuda_2014_J.Am.Chem.Soc_136_15326
PubMedSearch : Matsuda_2014_J.Am.Chem.Soc_136_15326
PubMedID: 25216349
Gene_locus related to this paper: emeva-andm

Title : Structures of D14 and D14L in the strigolactone and karrikin signaling pathways - Kagiyama_2013_Genes.Cells_18_147
Author(s) : Kagiyama M , Hirano Y , Mori T , Kim SY , Kyozuka J , Seto Y , Yamaguchi S , Hakoshima T
Ref : Genes Cells , 18 :147 , 2013
Abstract : Strigolactones (SLs) are plant hormones that inhibit shoot branching. DWARF14 (D14) inhibits rice tillering and is an SL receptor candidate in the branching inhibition pathway, whereas the close homologue DWARF14-LIKE (D14L) participates in the signaling pathway of karrikins (KARs), which are derived from burnt vegetation as smoke stimulants of seed germination. We provide the first evidence for direct binding of the bioactive SL analogue GR24 to D14. Isothermal titration calorimetry measurements show a D14-GR24 binding affinity in the sub-micromolar range. Similarly, bioactive KAR1 directly binds D14L in the micromolar range. The crystal structure of rice D14 shows a compact alpha-/beta-fold hydrolase domain forming a deep ligand-binding pocket capable of accommodating GR24. Insertion of four alpha-helices between beta6 strand and alphaD helix forms the helical cap of the pocket, although the pocket is open to the solvent. The pocket contains the conserved catalytic triad Ser-His-Asp aligned with the oxyanion hole, suggesting hydrolase activity. Although these structural characteristics are conserved in D14L, the D14L pocket is smaller than that of D14. The KAR-insensitive mutation kai2-1 is located at the prominent long beta6-alphaD1 loop, which is characteristic in D14 and D14L, but not in related alpha-/beta-fold hydrolases.
ESTHER : Kagiyama_2013_Genes.Cells_18_147
PubMedSearch : Kagiyama_2013_Genes.Cells_18_147
PubMedID: 23301669
Gene_locus related to this paper: arath-KAI2.D14L , orysj-Q10QA5

Title : Cotinine reduces amyloid-beta aggregation and improves memory in Alzheimer's disease mice - Echeverria_2011_J.Alzheimers.Dis_24_817
Author(s) : Echeverria V , Zeitlin R , Burgess S , Patel S , Barman A , Thakur G , Mamcarz M , Wang L , Sattelle DB , Kirschner DA , Mori T , Leblanc RM , Prabhakar R , Arendash GW
Ref : J Alzheimers Dis , 24 :817 , 2011
Abstract : Alzheimer's disease (AD) affects millions of people world-wide and new effective and safe therapies are needed. Cotinine, the main metabolite of nicotine, has a long half-life and does not have cardiovascular or addictive side effects in humans. We studied the effect of cotinine on amyloid-beta (Abeta) aggregation as well as addressed its impact on working and reference memories. Cotinine reduced Abeta deposition, improved working and reference memories, and inhibited Abeta oligomerization in the brains of transgenic (Tg) 6799 AD mice. In vitro studies confirmed the inhibitory effect of cotinine on Abeta1-42 aggregation. Cotinine stimulated Akt signaling, including the inhibition of glycogen synthase kinase 3beta (GSK3beta), which promotes neuronal survival and the synaptic plasticity processes underlying learning and memory in the hippocampus and cortex of wild type and Tg6799 AD mice. Simulation of the cotinine-Abeta1-42 complex using molecular dynamics showed that cotinine may interact with key histidine residues of Abeta1-42, altering its structure and inhibiting its aggregation. The good safety profile in humans and its beneficial effects suggest that cotinine may be an excellent therapeutic candidate for the treatment of AD.
ESTHER : Echeverria_2011_J.Alzheimers.Dis_24_817
PubMedSearch : Echeverria_2011_J.Alzheimers.Dis_24_817
PubMedID: 21321389

Title : Novel cation-pi interaction revealed by crystal structure of thermoalkalophilic lipase -
Author(s) : Matsumura H , Yamamoto T , Leow ATC , Mori T , Salleh AB , Basri M , Inoue T , Kai Y , Rahman RNZRA
Ref : Proteins , 70 :592 , 2008
PubMedID: 17932933
Gene_locus related to this paper: bacsp-lip

Title : A metagenomic approach to the identification of UDP-glucose 4-epimerase as a menadione resistance protein - Mori_2008_Biosci.Biotechnol.Biochem_72_1611
Author(s) : Mori T , Suenaga H , Miyazaki K
Ref : Biosci Biotechnol Biochem , 72 :1611 , 2008
Abstract : Quinones are potentially toxic agents that generate reactive oxygen species (ROS) upon reduction. We screened a metagenomic library for a menadione resistance gene to identify UDP-glucose 4-epimerase (UGE). Escherichia coli carrying the gene became resistant to various quinones, but not to ROS. Because UGE is involved in the biosynthesis of lipopolysaccharides, it may have contributed to forming a permeability barrier against lipophilic quinones.
ESTHER : Mori_2008_Biosci.Biotechnol.Biochem_72_1611
PubMedSearch : Mori_2008_Biosci.Biotechnol.Biochem_72_1611
PubMedID: 18540088

Title : Correlation of visual hallucinations with occipital rCBF changes by donepezil in DLB - Mori_2006_Neurology_66_935
Author(s) : Mori T , Ikeda M , Fukuhara R , Nestor PJ , Tanabe H
Ref : Neurology , 66 :935 , 2006
Abstract : The authors explored the neural substrate of visual hallucinations in dementia with Lewy bodies (DLB) by investigating changes in regional cerebral blood flow (rCBF) and psychiatric symptoms, before and after cholinesterase inhibitor treatment. Twenty subjects with DLB were treated with donepezil for a 12-week period. Hallucinations attenuated while receiving therapy, whereas occipital rCBF focally increased, suggesting that functional visual association cortex deficits may cause visual hallucinations in patients with DLB.
ESTHER : Mori_2006_Neurology_66_935
PubMedSearch : Mori_2006_Neurology_66_935
PubMedID: 16567718

Title : Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D - Matsuzaki_2004_Nature_428_653
Author(s) : Matsuzaki M , Misumi O , Shin IT , Maruyama S , Takahara M , Miyagishima SY , Mori T , Nishida K , Yagisawa F , Yoshida Y , Nishimura Y , Nakao S , Kobayashi T , Momoyama Y , Higashiyama T , Minoda A , Sano M , Nomoto H , Oishi K , Hayashi H , Ohta F , Nishizaka S , Haga S , Miura S , Morishita T , Kabeya Y , Terasawa K , Suzuki Y , Ishii Y , Asakawa S , Takano H , Ohta N , Kuroiwa H , Tanaka K , Shimizu N , Sugano S , Sato N , Nozaki H , Ogasawara N , Kohara Y , Kuroiwa T
Ref : Nature , 428 :653 , 2004
Abstract : Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support the lives of photosynthetic eukaryotes, including higher plants. Here we report the 16,520,305-base-pair sequence of the 20 chromosomes of the unicellular red alga Cyanidioschyzon merolae 10D as the first complete algal genome. We identified 5,331 genes in total, of which at least 86.3% were expressed. Unique characteristics of this genomic structure include: a lack of introns in all but 26 genes; only three copies of ribosomal DNA units that maintain the nucleolus; and two dynamin genes that are involved only in the division of mitochondria and plastids. The conserved mosaic origin of Calvin cycle enzymes in this red alga and in green plants supports the hypothesis of the existence of single primary plastid endosymbiosis. The lack of a myosin gene, in addition to the unexpressed actin gene, suggests a simpler system of cytokinesis. These results indicate that the C. merolae genome provides a model system with a simple gene composition for studying the origin, evolution and fundamental mechanisms of eukaryotic cells.
ESTHER : Matsuzaki_2004_Nature_428_653
PubMedSearch : Matsuzaki_2004_Nature_428_653
PubMedID: 15071595
Gene_locus related to this paper: cyam1-m1vi61 , cyam1-m1vhh9

Title : Noradrenergic and cholinergic nerves in the uterus of the Japanese long-fingered bat, Miniopterus schreibersii fuliginosus, change with reproductive cycle - Sugasawa_2002_Zoolog.Sci_19_387
Author(s) : Sugasawa K , Ando K , Mori T
Ref : Zoolog Sci , 19 :387 , 2002
Abstract : The pattern of uterine innervation by noradrenergic (NA) and acetylcholinesterase-positive (AChE) nerves in different reproductive stages of the adult Japanese long-fingered bats were investigated histochemically and immunohistochemically. In the non-pregnant bat, the uterine horn was supplied with abundant NA and AChE nerves. These two types of nerves were closely associated with the uterine arteries and myometrial smooth muscles. In the pregnant bat, NA and AChE nerves supplying the uterus did not degenerate much during hibernating period, but reduced markedly after arousal. In the postpartum bat, the density of nerves recovered progressively. The significant change in the innervation pattern of uterine NA and AChE nerves in the pregnant bats under and after hibernation, and in the postpartum bat must be considered in relation to the adrenergic and cholinergic controlling mechanisms on the uterine function that is matched for the unique reproductive cycle of this bat.
ESTHER : Sugasawa_2002_Zoolog.Sci_19_387
PubMedSearch : Sugasawa_2002_Zoolog.Sci_19_387
PubMedID: 12130815

Title : A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map - Aiba_1996_DNA.Res_3_363
Author(s) : Aiba H , Baba T , Hayashi K , Inada T , Isono K , Itoh T , Kasai H , Kashimoto K , Kimura S , Kitakawa M , Kitagawa M , Makino K , Miki T , Mizobuchi K , Mori H , Mori T , Motomura K , Nakade S , Nakamura Y , Nashimoto H , Nishio Y , Oshima T , Saito N , Sampei G , Horiuchi T , et al.
Ref : DNA Research , 3 :363 , 1996
Abstract : The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid beta-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.
ESTHER : Aiba_1996_DNA.Res_3_363
PubMedSearch : Aiba_1996_DNA.Res_3_363
PubMedID: 9097039
Gene_locus related to this paper: ecoli-ycjy