Fukui K

References (15)

Title : Desmethyl type germinone, a specific agonist for the HTL\/KAI2 receptor, induces the Arabidopsis seed germination in a gibberellin-independent manner - Okabe_2023_Biochem.Biophys.Res.Commun_649_110
Author(s) : Okabe S , Kitaoka K , Suzuki T , Kuruma M , Hagihara S , Yamaguchi S , Fukui K , Seto Y
Ref : Biochemical & Biophysical Research Communications , 649 :110 , 2023
Abstract : DWARF14 (D14) and HTL/KAI2 (KAI2) are paralogous receptors in the alpha/beta-hydrolase superfamily. D14 is the receptor for a class of plant hormones, strigolactones (SLs), and KAI2 is the receptor for the smoke-derived seed germination inducer, Karrikin (KAR), in Arabidopsis. Germinone (Ger) was previously reported as a KAI2 agonist with germination-inducing activity for thermo-inhibited Arabidopsis seed. However, Ger was not specific to KAI2, and could also bind to D14. It was reported that SL analogs with a desmethyl-type D-ring structure are specifically recognized by KAI2. On the basis of this observation, we synthesized a desmethyl-type germinone (dMGer). We found that dMGer is highly specific to KAI2. Moreover, dMGer induced Arabidopsis seed germination more effectively than did Ger. In addition, dMGer induced the seed germination of Arabidopsis in a manner independently of GA, a well-known germination inducer in plants.
ESTHER : Okabe_2023_Biochem.Biophys.Res.Commun_649_110
PubMedSearch : Okabe_2023_Biochem.Biophys.Res.Commun_649_110
PubMedID: 36764113

Title : Ligand-receptor interactions in plant hormone signaling - Takeuchi_2021_Plant.J_105_290
Author(s) : Takeuchi J , Fukui K , Seto Y , Takaoka Y , Okamoto M
Ref : Plant J , 105 :290 , 2021
Abstract : Small-molecule plant hormones principally control plant growth, development, differentiation, and environmental responses. Nine types of plant hormones are ubiquitous in angiosperms, and the molecular mechanisms of their hormone actions have been elucidated during the last two decades by genomic decoding of model plants with genetic mutants. In particular, the discovery of hormone receptors has greatly contributed to the understanding of signal transduction systems. The three-dimensional structure of the ligand-receptor complex has been determined for eight of the nine hormones by X-ray crystal structure analysis, and ligand perception mechanisms have been revealed at the atomic level. Collective research has revealed the molecular function of plant hormones that act as either molecular glue or an allosteric regulator for activation of receptors. In this review, we present an overview of the respective hormone signal transduction and describe the structural bases of ligand-receptor interactions.
ESTHER : Takeuchi_2021_Plant.J_105_290
PubMedSearch : Takeuchi_2021_Plant.J_105_290
PubMedID: 33278046

Title : On improving strigolactone mimics for induction of suicidal germination of the root parasitic plant Striga hermonthica - Takahashi_2021_aBIOTECH_2_1
Author(s) : Takahashi I , Fukui K , Asami T
Ref : aBIOTECH , 2 :1 , 2021
Abstract : Strigolactones (SLs) are plant hormones that regulate the branching of plants and seed germination stimulants of root parasitic plants. As root parasites are a great threat to agricultural production, the use of SL agonists could be anticipated to provide an efficient method for regulating root parasites as suicidal germination inducers. A series of phenoxyfuranone-type SL mimics, termed debranones, has been reported to show potent bioactivities, including reduction of the tiller number on rice, and stimulation of seed germination in the root parasite Striga hermonthica. To exert both activities, two substituents on the phenyl ring of the molecules were important but at least a substituent at the 2-position must be an electron-withdrawing group. However, little is known about the effect of the properties of the substituents at the 2-position on bioactivities. Here, we found that different substituents at the 2-position give different preferences for bioactivities. Halogenated debranones were more effective than the others and SL agonist GR24 for inhibiting rice tiller but far less effective in the induction of S. hermonthica germination. Meanwhile, nitrile and methyl derivatives clearly stimulated the germination of S. hermonthica seeds. Although their IC50 values were 100 times higher than that of GR24 in the receptor competitive binding assay, their physiological activities were approximately 1/10 of GR24. These differences could be due to their uptake in plants and/or their physicochemical stability under our experimental conditions. These findings could support the design of more potent and selective SL agonists that could contribute to solving big agricultural issues.
ESTHER : Takahashi_2021_aBIOTECH_2_1
PubMedSearch : Takahashi_2021_aBIOTECH_2_1
PubMedID:

Title : Explicating anti-amyloidogenic role of curcumin and piperine via amyloid beta (Abeta) explicit pathway: recovery and reversal paradigm effects - Abdul_2020_PeerJ_8_e10003
Author(s) : Abdul Manap AS , Madhavan P , Vijayabalan S , Chia A , Fukui K
Ref : PeerJ , 8 :e10003 , 2020
Abstract : Previously, we reported the synergistic effects of curcumin and piperine in cell cultures as potential anti-cholinesterase and anti-amyloidogenic agents. Due to limited findings on the enrolment of these compounds on epigenetic events in AD, we aimed at elucidating the expression profiles of Abeta42-induced SH-SY5Y cells using microarray profiling. In this study, an optimized concentration of 35 muM of curcumin and piperine in combination was used to treat Abeta42 fibril and high-throughput microarray profiling was performed on the extracted RNA. This was then compared to curcumin and piperine used singularly at 49.11 muM and 25 muM, respectively. Our results demonstrated that in the curcumin treated group, from the top 10 upregulated and top 10 downregulated significantly differentially expressed genes (p < 0.05; fold change >= 2 or <= -2), there were five upregulated and three downregulated genes involved in the amyloidogenic pathway. While from top 10 upregulated and top 10 downregulated significantly differentially expressed genes (p < 0.05; fold change >= 2 or <= - 2) in the piperine treated group, there were four upregulated and three downregulated genes involved in the same pathway, whereas there were five upregulated and two downregulated genes involved (p < 0.05; fold change >= 2 or <= - 2) in the curcumin-piperine combined group. Four genes namely GABARAPL1, CTSB, RAB5 and AK5 were expressed significantly in all groups. Other genes such as ITPR1, GSK3B, PPP3CC, ERN1, APH1A, CYCS and CALM2 were novel putative genes that are involved in the pathogenesis of AD. We revealed that curcumin and piperine have displayed their actions against Abeta via the modulation of various mechanistic pathways. Alterations in expression profiles of genes in the neuronal cell model may explain Abeta pathology post-treatment and provide new insights for remedial approaches of a combined treatment using curcumin and piperine.
ESTHER : Abdul_2020_PeerJ_8_e10003
PubMedSearch : Abdul_2020_PeerJ_8_e10003
PubMedID: 33062432

Title : Synthetic agonist of HTL\/KAI2 shows potent stimulating activity for Arabidopsis seed germination - Fukui_2019_Bioorg.Med.Chem.Lett_29_2487
Author(s) : Fukui K , Arai K , Kasahara H , Asami T , Hayashi KI
Ref : Bioorganic & Medicinal Chemistry Lett , 29 :2487 , 2019
Abstract : HTL/KAI2, a member of the alpha/beta-fold hydrolase superfamily, is known to be a receptor-like protein of lactone compounds and that triggers seed germination of Arabidopsis. However, the endogenous ligand and physiological roles of HTL/KAI2 have remained unclear. To understand the mechanism underlying seed germination involved in HTL/KAI2 signaling, it is necessary to identify the endogenous ligand of HTL/KAI2. To date, even a biosynthetic mutant of the ligand has not yet been isolated. Because exogenous agonistic chemicals can only be purchased in small amounts at high prices, the limited supply of those chemicals has hampered any large-scale experiments, such as mutant screening. Therefore, easily synthesized and scalable artificial agonist would remove the limitation of the chemical supply and contribute to the identification of the endogenous ligand of HTL/KAI2 and/or the biosynthetic mutants. In this study, we demonstrated that designed chemicals with a phenoxyfuranone scaffold potently stimulated seed germination via HTL/KAI2 in Arabidopsis. As a result of screening of these chemicals, we selected a representative compound with convincing selectivity. Here in, we provide a new promising synthetic agonist of HTL/KAI2.
ESTHER : Fukui_2019_Bioorg.Med.Chem.Lett_29_2487
PubMedSearch : Fukui_2019_Bioorg.Med.Chem.Lett_29_2487
PubMedID: 31345632
Gene_locus related to this paper: arath-KAI2.D14L

Title : A Taylor-Made Design of Phenoxyfuranone-Type Strigolactone Mimic - Fukui_2017_Front.Plant.Sci_8_936
Author(s) : Fukui K , Yamagami D , Ito S , Asami T
Ref : Front Plant Sci , 8 :936 , 2017
Abstract : Strigolactones are a class of plant hormones that inhibit axillary bud outgrowth and are released from plant roots to act as a rhizosphere communication signal. The Orobanchaceae parasitic plant Striga hermonthica perceives strigolactone as its germination signal, indicating host presence. After germination, the Striga plant parasitises the host plant and suppresses host growth by draining photosynthetic products, water and other essential nutrients. Because of this way of life, this parasite threatens crop production in sub-Saharan Africa with infestation in crop fields and crop devastation. Crop protection in such areas is among the most concerning problems to be dealt with as immediately as possible. With respect to crop protection from Striga, many strigolactone agonists have been developed and used in research to reveal Striga biology, and have contributed to development of crop protection methods. However, an effective method has yet to be found. In a previous study, we reported debranones as a group of strigolactone mimics that inhibit axillary buds outgrowth with moderate stimulation activity for Striga germination. Debranones would be accessible because they are simply synthesized from commercially available phenols and bromo butenolide. Taking this advantage of debranones for Striga research, we tried to find the debranones stimulating Striga seed germination. To modulate functional selectivity and to enhance germination inducing activity of debranones, we studied structure-activity relationships. We investigated effects of substituent position and functional group on debranone activity and selectivity as a strigolactone mimic. As a result, we improved stimulation activity of debranones for Striga seed germination by chemical modification, and demonstrated the pharmacophore of debranones for selective modulation of distinct strigolactone responses.
ESTHER : Fukui_2017_Front.Plant.Sci_8_936
PubMedSearch : Fukui_2017_Front.Plant.Sci_8_936
PubMedID: 28676802

Title : Cognitive Impairments Induced by Concussive Mild Traumatic Brain Injury in Mouse Are Ameliorated by Treatment with Phenserine via Multiple Non-Cholinergic and Cholinergic Mechanisms - Tweedie_2016_PLoS.One_11_e0156493
Author(s) : Tweedie D , Fukui K , Li Y , Yu QS , Barak S , Tamargo IA , Rubovitch V , Holloway HW , Lehrmann E , Wood WH, 3rd , Zhang Y , Becker KG , Perez E , Van Praag H , Luo Y , Hoffer BJ , Becker RE , Pick CG , Greig NH
Ref : PLoS ONE , 11 :e0156493 , 2016
Abstract : Traumatic brain injury (TBI), often caused by a concussive impact to the head, affects an estimated 1.7 million Americans annually. With no approved drugs, its pharmacological treatment represents a significant and currently unmet medical need. In our prior development of the anti-cholinesterase compound phenserine for the treatment of neurodegenerative disorders, we recognized that it also possesses non-cholinergic actions with clinical potential. Here, we demonstrate neuroprotective actions of phenserine in neuronal cultures challenged with oxidative stress and glutamate excitotoxicity, two insults of relevance to TBI. These actions translated into amelioration of spatial and visual memory impairments in a mouse model of closed head mild TBI (mTBI) two days following cessation of clinically translatable dosing with phenserine (2.5 and 5.0 mg/kg BID x 5 days initiated post mTBI) in the absence of anti-cholinesterase activity. mTBI elevated levels of thiobarbituric acid reactive substances (TBARS), a marker of oxidative stress. Phenserine counteracted this by augmenting homeostatic mechanisms to mitigate oxidative stress, including superoxide dismutase [SOD] 1 and 2, and glutathione peroxidase [GPx], the activity and protein levels of which were measured by specific assays. Microarray analysis of hippocampal gene expression established that large numbers of genes were exclusively regulated by each individual treatment with a substantial number of them co-regulated between groups. Molecular pathways associated with lipid peroxidation were found to be regulated by mTBI, and treatment of mTBI animals with phenserine effectively reversed injury-induced regulations in the 'Blalock Alzheimer's Disease Up' pathway. Together these data suggest that multiple phenserine-associated actions underpin this compound's ability to ameliorate cognitive deficits caused by mTBI, and support the further evaluation of the compound as a therapeutic for TBI.
ESTHER : Tweedie_2016_PLoS.One_11_e0156493
PubMedSearch : Tweedie_2016_PLoS.One_11_e0156493
PubMedID: 27254111

Title : Discovery and identification of 2-methoxy-1-naphthaldehyde as a novel strigolactone-signaling inhibitor - Mashita_2016_J.Pestic.Sci_41_71
Author(s) : Mashita O , Koishihara H , Fukui K , Nakamura H , Asami T
Ref : J Pestic Sci , 41 :71 , 2016
Abstract : Knowledge about strigolactone biosynthesis and signaling is increasing and the crystal structure of strigolactone receptor protein D14 has been resolved. Although a variety of strigolactone biosynthesis inhibitors and strigolactone agonists are known, no inhibitors of strigolactone signaling have been reported. Here, we conducted virtual screening in silico to identify chemical regulators that inhibit SL reception. We used LigandScout to analyze a pharmacophore model based on structural information about D14 protein and complex D14-D-OH (a hydrolysis product of strigolactone formed by D14). We identified a candidate compound, XM-47, and confirmed that it inhibits D14-SLR1 and D14-D53 interactions. A possible product of XM-47 hydrolysis, 2-methoxy-1-naphthaldehyde (2-MN), inhibits D14-SLR1 and D14-D53 interactions and restores the growth of rice tillering buds suppressed by strigolactone.
ESTHER : Mashita_2016_J.Pestic.Sci_41_71
PubMedSearch : Mashita_2016_J.Pestic.Sci_41_71
PubMedID: 30363101

Title : Chemical modification of a phenoxyfuranone-type strigolactone mimic for selective effects on rice tillering or Striga hermonthica seed germination - Takahashi_2016_Pest.Manag.Sci_72_2048
Author(s) : Takahashi I , Fukui K , Asami T
Ref : Pest Manag Sci , 72 :2048 , 2016
Abstract : BACKGROUND: We previously reported that a series of phenoxyfuranone compounds, designated 'debranones', mimic strigolactone (SL) activity. 4-Bromodebranone (4BD) is a functionally selective SL mimic that reduces the number of shoot branches on rice more potently than GR24, a typical synthetic SL analogue, but does not induce seed germination in the root-parasitic plant Striga hermonthica. To enhance the selective activity of debranones in stimulating the seed germination of root-parasitic plants, we prepared several analogues of 4BD in which the chlorine atom was substituted with an H atom at the o-, m- or p-position on the phenyl ring (designated 2-, 3-, or 4-chlorodebranone, respectively) or had a bicyclic group instead of the phenyl ring. We evaluated the biological activities of the compounds with rice tillering assays and S. hermonthica seed germination assays. RESULTS: Both assays showed that the substituent position affected debranone efficiency, and among the monochlorodebranones, 2-chlorodebranone was more effective than the other two isomers in both assays. When the activities of the bicyclic debranones were compared in the same two assays, one was more active than GR24 in the rice tillering assay. This debranone also stimulated the germination of S. hermonthica seeds. Thus, some debranone derivatives induced the germination of S. hermonthica seeds, although their activities were still approximately 1/20 that of GR24. CONCLUSION: These results strongly suggest that further and rigorous structure-activity relationship studies of the debranones will identify derivatives that more potently stimulate the suicidal germination of S. hermonthica seeds. (c) 2016 Society of Chemical Industry.
ESTHER : Takahashi_2016_Pest.Manag.Sci_72_2048
PubMedSearch : Takahashi_2016_Pest.Manag.Sci_72_2048
PubMedID: 26929041

Title : Molecular mechanism of strigolactone perception by DWARF14 - Nakamura_2013_Nat.Commun_4_2613
Author(s) : Nakamura H , Xue YL , Miyakawa T , Hou F , Qin HM , Fukui K , Shi X , Ito E , Ito S , Park SH , Miyauchi Y , Asano A , Totsuka N , Ueda T , Tanokura M , Asami T
Ref : Nat Commun , 4 :2613 , 2013
Abstract : Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.
ESTHER : Nakamura_2013_Nat.Commun_4_2613
PubMedSearch : Nakamura_2013_Nat.Commun_4_2613
PubMedID: 24131983
Gene_locus related to this paper: orysj-Q10QA5

Title : New branching inhibitors and their potential as strigolactone mimics in rice - Fukui_2011_Bioorg.Med.Chem.Lett_21_4905
Author(s) : Fukui K , Ito S , Ueno K , Yamaguchi S , Kyozuka J , Asami T
Ref : Bioorganic & Medicinal Chemistry Lett , 21 :4905 , 2011
Abstract : Strigolactones (SLs) are rhizosphere communication chemicals. Recent studies of highly branched mutants revealed that SL or its metabolites work as a phytohormone to inhibit shoot branching. When SLs are exogenously applied to the rice d10-1 mutant that has a highly branched phenotype caused by a defect in the SL biosynthesis gene (CCD8), they inhibit tiller bud outgrowth (branching in rice) of the mutant. We focused our attention on the SL function as a phytohormone and tried to find new chemicals mimicking the hormonal action of SL by screening chemicals that inhibit branching of rice d10-1 mutant. Fortunately, we found 5-(4-chlorophenoxy)-3-methylfuran-2(5H)-one (3a) as a new chemical possessing SL-like activity against the rice d10-1 mutant. Then, we prepared several derivatives of 3a (3b-3k) to examine their ability to inhibit shoot branching of rice d10-1. These derivatives were synthesized by a one-pot coupling reaction between phenols and halo butenolide to give 5-phenoxy 3-methylfuran-2(5H)-one (3) derivatives, which possess a common substructure with SLs. Some of the derivatives showed SL-like activity more potently than GR24, a typical SL derivative, in a rice assay. As SLs also show activity by inducing seed germination of root parasitic plants, the induction activity of these derivatives was also evaluated. Here we report the structure-activity relationships of these compounds.
ESTHER : Fukui_2011_Bioorg.Med.Chem.Lett_21_4905
PubMedSearch : Fukui_2011_Bioorg.Med.Chem.Lett_21_4905
PubMedID: 21741836

Title : Increased level of pericardial insulin-like growth factor-1 in patients with left ventricular dysfunction and advanced heart failure - Abe_2006_J.Am.Coll.Cardiol_48_1387
Author(s) : Abe N , Matsunaga T , Kameda K , Tomita H , Fujiwara T , Ishizaka H , Hanada H , Fukui K , Fukuda I , Osanai T , Okumura K
Ref : J Am Coll Cardiol , 48 :1387 , 2006
Abstract : OBJECTIVES: To test the hypothesis that the cardiac insulin-like growth factor-1 (IGF-1) system is up-regulated in the failing heart, we measured the pericardial (cardiac) and plasma (circulating) IGF-1 levels in coronary artery disease patients. BACKGROUND: Local IGF-1 systems are regulated differently from the systemic IGF-1 system. The cardiac IGF-1 system is up-regulated by the increased left ventricular (LV) wall stress. However, it remains unknown how this system is affected in LV dysfunction and heart failure.
METHODS: We measured the plasma and pericardial fluid levels of IGF-1 and brain natriuretic peptide (BNP) in 87 coronary artery disease patients undergoing cardiac surgery, and examined their relationships with LV function and heart failure severity. The expressions of IGF-1 and IGF-1 receptor proteins were examined in endomyocardial biopsies obtained from other patients with normal or impaired LV function.
RESULTS: The pericardial IGF-1 and BNP levels were positively correlated with the plasma BNP level (both p < 0.001). The pericardial IGF-1 level was increased in heart failure patients, whereas the plasma IGF-1 level was rather decreased. The pericardial IGF-1 level was inversely correlated with the LV ejection fraction (p < 0.001), whereas the plasma IGF-1 level was not. Positive immunostaining for IGF-1 and IGF-1 receptor proteins was enhanced in myocardial biopsies from failing hearts compared with those from nonfailing hearts.
CONCLUSIONS: The pericardial IGF-1 level was increased in patients with LV dysfunction and heart failure, whereas the plasma IGF-1 level was decreased. These results may indicate that up-regulation of the cardiac IGF-1 system serves as a compensatory mechanism for LV dysfunction.
ESTHER : Abe_2006_J.Am.Coll.Cardiol_48_1387
PubMedSearch : Abe_2006_J.Am.Coll.Cardiol_48_1387
PubMedID: 17010800

Title : Clinical features of nicotine dependence compared with those of alcohol, methamphetamine, and inhalant dependence - Miyata_2004_Ann.N.Y.Acad.Sci_1025_481
Author(s) : Miyata H , Kono J , Ushijima S , Yanagita T , Miyasato K , Fukui K
Ref : Annals of the New York Academy of Sciences , 1025 :481 , 2004
Abstract : A new clinical evaluation form was developed to compare the clinical features of nicotine dependence with those associated with other abused drugs. A new scoring system for clinical evaluation was developed. The form consisted of five scoring items: subjective effects, liking (of drug), withdrawal syndrome, acute psychic and physical disorders, and social disturbance. A preliminary clinical investigation was performed to test the validity of the evaluation form. Study subjects were those showing dependence on nicotine (cigarette smoking, n = 40), alcohol (n = 39), methamphetamine (n = 31), and inhalants (n = 30), who fulfilled the DSM-IV-TR criteria for drug dependence disregarding the state of "a maladaptive pattern of substance use, leading to clinically significant impairment or distress," and gave written informed consent for participation in the study. Nicotine caused a mild or the least degree of subjective effects, liking, and psychic and physical withdrawal symptoms, without any significant social disturbance or acute disorders. With alcohol, liking, withdrawal syndrome, and acute physical disorders were prominent. Methamphetamine produced the most serious acute psychic disorders, with intensive acute physical disorders and psychic withdrawal symptoms. Inhalants were characterized by an intensive degree of acute psychic disorders. As for social disturbance, alcohol, methamphetamine, and inhalants showed more significant influence than nicotine. Our study findings revealed that the clinical features of drug dependence could be evaluated by using the new clinical evaluation form. Further study is required to clarify the clinical features of nicotine dependence compared with those of other drugs of dependence.
ESTHER : Miyata_2004_Ann.N.Y.Acad.Sci_1025_481
PubMedSearch : Miyata_2004_Ann.N.Y.Acad.Sci_1025_481
PubMedID: 15542752

Title : [Studies on clinical characteristics of nicotine dependence using a two compartment model of drug dependence] - Miyata_2004_Nihon.Shinkei.Seishin.Yakurigaku.Zasshi_24_61
Author(s) : Miyata H , Kono J , Yanagita T , Ushijima S , Miyasato K , Fukui K
Ref : Nihon Shinkei Seishin Yakurigaku Zasshi , 24 :61 , 2004
Abstract : The purpose of the present study was to develop a new clinical evaluation form to compare the clinical characteristics of nicotine dependence with those associated with other drugs of abuse, using a two-compartment model consisting of "drug dependence" and "dependence syndrome". The evaluation form consisted of five scoring items: subjective effects, drug liking, withdrawal syndrome, acute psychic and acute physical disorders, and social disturbance. "Drug dependence" was defined by positive scores on the "drug liking" item. "Dependence syndrome" was defined by positive scores on drug-induced pathological symptoms (withdrawal syndrome, and acute psychic and physical disorders) and social disturbance. The subjects were dependent on nicotine (cigarette smoking) (n = 114), alcohol (n = 101), methamphetamine (n = 90), inhalants (n = 63), and benzodiazepines (n = 39). All subjects met the DSM-IV-TR criteria for drug dependence. Nicotine produced a mild or the least degree of drug liking and withdrawal syndrome, without any significant social disturbance, or acute disorders. The other four drugs produced more intensive degrees of withdrawal syndrome and acute psychic and physical symptoms, with more significant social disturbance than nicotine. The present study indicated that nicotine dependence differed from other forms of drug dependence in that nicotine was not associated with "dependence syndrome".
ESTHER : Miyata_2004_Nihon.Shinkei.Seishin.Yakurigaku.Zasshi_24_61
PubMedSearch : Miyata_2004_Nihon.Shinkei.Seishin.Yakurigaku.Zasshi_24_61
PubMedID: 15164611

Title : Enzyme-linked immunosorbent assay (ELISA) for Aleuria aurantia lectin-reactive serum cholinesterase to differentiate liver cirrhosis and chronic hepatitis - Kondo_1995_Clin.Chim.Acta_243_1
Author(s) : Kondo M , Hada T , Fukui K , Iwasaki A , Higashino K , Yasukawa K
Ref : Clinica Chimica Acta , 243 :1 , 1995
Abstract : We have established an enzyme-linked immunosorbent assay (ELISA) for total serum cholinesterase (ChE) using 2 new monoclonal antibodies (mAbs) to ChE (E.C.3.1.1.8). The ELISA results correlated very well with the results of a serum ChE activity assay, which has been widely used for differentiating patients with liver diseases, such as hepatocellular carcinoma, liver cirrhosis and chronic hepatitis, from normal individuals. We next established an ELISA for Aleuria aurantia lectin (AAL)-reactive serum ChE using one of the anti-ChE mAbs and AAL, which specifically recognizes L-fucose alpha 1-->2, L-fucose alpha 1-->3, and L-fucose alpha 1-->6 structures. The ratio of AAL-reactive ChE to total ChE in sera determined by the two ELISA procedures was increased in patients with hepatocellular carcinoma and liver cirrhosis compared with patients with chronic hepatitis and normal individuals. We then applied the ELISA for AAL-reactive ChE directly to 10-fold-diluted serum samples, and by using a cut-off value of the mean + 2S.D. for normal individuals, we could effectively differentiate liver cirrhosis from chronic hepatitis. This single ELISA for AAL-reactive ChE could be a useful aid in clinical diagnosis.
ESTHER : Kondo_1995_Clin.Chim.Acta_243_1
PubMedSearch : Kondo_1995_Clin.Chim.Acta_243_1
PubMedID: 8747509