Leal DB

References (9)

Title : Neuroprotective effects of pretreatment with quercetin as assessed by acetylcholinesterase assay and behavioral testing in poloxamer-407 induced hyperlipidemic rats - Braun_2017_Biomed.Pharmacother_88_1054
Author(s) : Braun JB , Ruchel JB , Adefegha SA , Coelho AP , Trelles KB , Signor C , Rubin MA , Oliveira JS , Dornelles GL , de Andrade CM , Castilhos LG , Leal DB
Ref : Biomed Pharmacother , 88 :1054 , 2017
Abstract : Hyperlipidemia is a group of disorders characterized by excessive lipids in the bloodstream. It is associated with the incidence of cardiovascular diseases and recognized as the most important factor underlying the occurrence of atherosclerosis. This study was conducted to investigate whether pretreatment with quercetin can protect against possible memory impairment and deterioration of the cholinergic system in hyperlipidemic rats. Animals were divided into ten groups (n=7): saline/control, saline/quercetin 5mg/kg, saline/quercetin 25mg/kg, saline/quercetin 50mg/kg, saline/simvastatin (0.04mg/kg), hyperlipidemia, hyperlipidemia/quercetin 5mg/kg, hyperlipidemia/quercetin 25mg/kg, hyperlipidemia/quercetin 50mg/kg and hyperlipidemia/simvastatin. The animals were pretreated with quercetin by oral gavage for a period of 30days and hyperlipidemia was subsequently induced by intraperitoneal administration of a single dose of 500mg/kg of poloxamer-407. Simvastatin was administered after the induction of hyperlipidemia. The results demonstrated that hyperlipidemic rats had memory impairment compared with the saline control group (P<0.001). However, pretreatment with quercetin and simvastatin treatment attenuated the damage caused by hyperlipidemia compared with the hyperlipidemic group (P<0.05). Acetylcholinesterase (AChE) activity in the cerebral hippocampus was significantly (P<0.001) reduced in the hyperlipidemic group compared with the control saline group. Pretreatment with quercetin and simvastatin treatment in the hyperlipidemic groups significantly (P<0.05) increased AChE activity compared with the hyperlipidemic group. Our results thus suggest that quercetin may prevent memory impairment, alter lipid metabolism, and modulate AChE activity in an experimental model of hyperlipidemia.
ESTHER : Braun_2017_Biomed.Pharmacother_88_1054
PubMedSearch : Braun_2017_Biomed.Pharmacother_88_1054
PubMedID: 28192878

Title : Increased oxidative stress alters nucleosides metabolite levels in sickle cell anemia - Castilhos_2017_Redox.Rep__1
Author(s) : Castilhos LG , de Oliveira JS , Adefegha SA , Magni LP , Doleski PH , Abdalla FH , de Andrade CM , Leal DB
Ref : Redox Rep , :1 , 2017
Abstract : OBJECTIVES: This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients.
METHODS: Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCA patients.
RESULTS: ROS, thiobarbituric acid-reactive substances (TBARS) and T-SH levels as well as the activities of catalase and MPO were significantly increased while glutathione level was reduced in SCA patients. Furthermore, a significant (P < 0.001) increase in hypoxanthine level was demonstrated in SCA patients. However, the serum levels for xanthine (P < 0.01) and uric acid (P < 0.001) were decreased in SCA patients. A significant (P < 0.001) decrease in XO activity was detected in SCA patients. DISCUSSION: The altered parameters in SCA patients suggest that the generation and impairment of oxidative stress in this disease as well as antioxidant markers are contributory factors towards cellular redox homeostasis and alteration of purine metabolites.
ESTHER : Castilhos_2017_Redox.Rep__1
PubMedSearch : Castilhos_2017_Redox.Rep__1
PubMedID: 28209096

Title : Guarana (Paullinia cupana) ameliorates memory impairment and modulates acetylcholinesterase activity in Poloxamer-407-induced hyperlipidemia in rat brain - Ruchel_2016_Physiol.Behav_168_11
Author(s) : Ruchel JB , Braun JB , Adefegha SA , Guedes Manzoni A , Abdalla FH , de Oliveira JS , Trelles K , Signor C , Lopes ST , da Silva CB , Castilhos LG , Rubin MA , Leal DB
Ref : Physiol Behav , 168 :11 , 2016
Abstract : Hyperlipidemia is a risk factor for the development of cognitive dysfunction and atherosclerosis. Natural compounds have recently received special attention in relation to the treatment of disease due to their low cost and wide margin of safety. Thus, the aim of this study was to determine the possible preventive effect of guarana powder (Paullinia cupana) on memory impairment and acetylcholinesterase (AChE) activity in the brain structures of rats with Poloxamer-407-induced hyperlipidemia. Adult male Wistar rats were pretreated with guarana (12.5, 25 and 50mg/kg/day) and caffeine (0.2mg/kg/day) by gavage for a period of 30days. Simvastatin (0.04mg/kg) was administered as a comparative standard. Acute hyperlipidemia was induced with intraperitoneal injections of 500mg/kg of Poloxamer-407. Memory tests and evaluations of anxiety were performed. The cortex, cerebellum, hippocampus, hypothalamus and striatum were separated to assess acetylcholinesterase activity. Our results revealed that guarana powder was able to reduce the levels of TC and LDL-C in a manner similar to simvastatin. Guarana powder also partially reduced the liver damage caused by hyperlipidemia. Guarana was able to prevent changes in the activity of AChE and improve memory impairment due to hyperlipidemia. Guarana powder may therefore be a source of promising phytochemicals that can be used as adjuvant therapy in the management of hyperlipidemia and cognitive disorders.
ESTHER : Ruchel_2016_Physiol.Behav_168_11
PubMedSearch : Ruchel_2016_Physiol.Behav_168_11
PubMedID: 27720901

Title : Cholinesterase of rats experimentally infected by Cryptococcus neoformans: Relationship between inflammatory response and pathological findings - de Azevedo_2015_Pathol.Res.Pract_211_851
Author(s) : de Azevedo MI , Ferreiro L , Da Silva AS , Tonin AA , Thorstenberg ML , Catilhos LG , Franca RT , Leal DB , Duarte MM , Lopes ST , Sangoi MB , Moresco RN , Fighera R , Santurio JM
Ref : Pathol Res Pract , 211 :851 , 2015
Abstract : The aim of this study was to assess the role of the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as biomarkers of inflammation and tissue injury on rats experimentally infected by Cryptococcus neoformans. For this purpose, 20 male rats were divided into two groups: 10 animals representing the uninfected control group (Group A) and 10 C. neoformans var. grubii infected animals (Group B). Blood and brain samples were collected on days 10 (A10 and B10), and 30 (A30 and B30) post-infection (PI) for hematological analyses; AChE (in lymphocytes and brain) and seric BChE activity; interleukins (IL-1, IL-6, and IL-10); nitrite/nitrate (NOx) levels; and markers of protein oxidation (AOPP) and lipid peroxidation (TBARS). As a result, when animals of Group A were compared to animals of Group B, it was observed leukocytosis (P<0.05) on day 10 PI; AChE activity increase (P<0.05) in lymphocytes (day 30 PI) and in brain (days 10 and 30 PI); BChE activity decrease (P<0.05) on day 10 PI; IL-1 and IL-6 increase (P<0.01) in both periods, while IL-10 had reduced levels (P<0.01) in the same periods; NOx levels increased (P<0.05) significantly on days 10 and 30 PI, while AOPP and TBARS levels increased significantly on day 30 PI; as well as pneumonia on infected rats. Therefore, based on the results obtained, it was possible to conclude that AChE and BChE behavior lead to a proinflammatory reaction evidenced by the enhancement of IL-1, IL-6, and NOx throughout the experiment associated with reduction on IL-10 levels, and cellular damage.
ESTHER : de Azevedo_2015_Pathol.Res.Pract_211_851
PubMedSearch : de Azevedo_2015_Pathol.Res.Pract_211_851
PubMedID: 26376950

Title : Influence of Toxoplasma gondii Acute Infection on Cholinesterase Activities of Wistar Rats - Tonin_2013_Korean.J.Parasitol_51_421
Author(s) : Tonin AA , Da Silva AS , Thorstenberg ML , Castilhos LG , Franca RT , Leal DB , Duarte MM , Vogel FS , de La Rue ML , Dos Anjos Lopes ST
Ref : Korean J Parasitol , 51 :421 , 2013
Abstract : Several studies have shown the mechanisms and importance of immune responses against Toxoplasma gondii infection and the notable role of cholinesterases in inflammatory reactions. However, the association between those factors has not yet been investigated. Therefore, the aim of this study was to evaluate the acetylcholinesterase (AChE) activity in blood and lymphocytes and the activity of butyrylcholinesterase (BChE) in serum of rats experimentally infected with T. gondii during the acute phase of infection. For that, an in vivo study was performed with evaluations of AChE and BChE activities on days 5 and 10 post-infection (PI). The activity of AChE in blood was increased on day 5 PI, while in lymphocytes its activity was enhanced on days 5 and 10 PI (P<0.05). No significant difference was observed between groups regarding to the activity of BChE in serum. A positive (P<0.01) correlation was observed between AChE activity and number of lymphocytes. The role of AChE as an inflammatory marker is well known in different pathologies; thus, our results lead to the hypothesis that AChE has an important role in modulation of early immune responses against T. gondii infection.
ESTHER : Tonin_2013_Korean.J.Parasitol_51_421
PubMedSearch : Tonin_2013_Korean.J.Parasitol_51_421
PubMedID: 24039284

Title : Relationship between butyrylcholinesterase activity and liver injury in mice acute infected with Toxoplasma gondii - Da Silva_2013_Pathol.Res.Pract_209_95
Author(s) : Da Silva AS , Tonin AA , Thorstenberg ML , Leal DB , Fighera R , Flores MM , Franca RT , Camillo G , Vogel FS , de la Rue M , Lopes ST
Ref : Pathol Res Pract , 209 :95 , 2013
Abstract : This study aimed to investigate the butyrylcholinesterase (BChE) activity in mice experimentally infected with Toxoplasma gondii during the acute phase. Twenty mice were divided in two groups with 10 animals each: group A was composed of uninfected mice while group B was formed by rodents infected with T. gondii. Five days after infection, blood was collected and serum separated, and fragments of liver and brain were obtained. In serum and liver homogenate was noted a significant reduction (P<0.05) in BChE activity in infected mice when compared with uninfected ones. In serum was observed an increase in the activity of alanine aminotransferase and urea, associated with reduction in alkaline phosphatase activity and in the levels of total protein and albumin. Histologically, there were foci of necrosis and parasites in the forms of tachyzoites and cysts, with bradyzoites in liver samples of infected animals. Based on these results, we conclude that toxoplasmosis reduces BChE activity in mice, and this alteration is probably related to the liver damage caused by the parasitism. Therefore, this enzymatic alteration can directly contribute to the pathogenesis of the disease.
ESTHER : Da Silva_2013_Pathol.Res.Pract_209_95
PubMedSearch : Da Silva_2013_Pathol.Res.Pract_209_95
PubMedID: 23313104

Title : Ectoenzymes and cholinesterase activity and biomarkers of oxidative stress in patients with lung cancer - Zanini_2013_Mol.Cell.Biochem_374_137
Author(s) : Zanini D , Schmatz R , Pelinson LP , Pimentel VC , da Costa P , Cardoso AM , Martins CC , Schetinger CC , Baldissareli J , do Carmo Araujo M , Oliveira L , Chiesa J , Morsch VM , Leal DB , Schetinger MR
Ref : Molecular & Cellular Biochemistry , 374 :137 , 2013
Abstract : We aimed to examine the nucleoside triphosphate diphosphohydrolases (NTPDase) in lymphocytes; adenosine deaminase (ADA) and butyrylcholinesterase (BChE) in serum; and acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT) activity in whole blood; since these enzymes are involved in inflammation responses as well as in oxidative stress conditions. We also checked the levels of total thiols (T-SH), non-protein thiols (NPSH), and thiobarbituric acid reactive substances (TBARS) in serum of patients with lung cancer. We collected blood samples from patients (n = 31) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). The results showed a significant increase in the hydrolysis of ATP, ADP, and adenosine in patients when compared with the control group. The activity of AChE, SOD, and CAT as well as the T-SH and NPSH levels were higher in patients group and TBARS levels were lower in patients compared with the control group. These findings demonstrated that the enzymes activity involved in the control of inflammatory and immune processes as well as the oxidative stress parameters are altered in patients with lung cancer.
ESTHER : Zanini_2013_Mol.Cell.Biochem_374_137
PubMedSearch : Zanini_2013_Mol.Cell.Biochem_374_137
PubMedID: 23180243

Title : The effect of curcumin in the ectonucleotidases and acetylcholinesterase activities in synaptosomes from the cerebral cortex of cigarette smoke-exposed rats - Jaques_2011_Cell.Biochem.Funct_29_703
Author(s) : Jaques JA , Rezer JF , Goncalves JF , Spanevello RM , Gutierres JM , Pimentel VC , Thome GR , Morsch VM , Schetinger MR , Leal DB
Ref : Cell Biochemistry & Function , 29 :703 , 2011
Abstract : With the evidence that curcumin may be a potent neuroprotective agent and that cigarette smoke is associated with a decline in the cognitive performance as our bases, we investigated the activities of Ecto-Nucleoside Triphosphate Diphosphohydrolase (NTPDase), 5'-nucleotidase and acetylcholinesterase (AChE) in cerebral cortex synaptosomes from cigarette smoke-exposed rats treated with curcumin (Cur). The experimental procedures entailed two sets of experiments. In the first set, the groups were vehicle, Cur 12.5, 25 and 50 mg.kg(-1) ; those in the second set were vehicle, smoke, smoke and Cur 12.5, 25 and 50 mg.kg(-1) . Curcumin prevented the increased NTPDase, 5'-nucleotidase and AChE activities caused by smoke exposure. We suggest that treatment with Cur was protective because the decrease of ATP and acetylcholine (ACh) concentrations is responsible for cognitive impairment, and both ATP and ACh have key roles in neurotransmission.
ESTHER : Jaques_2011_Cell.Biochem.Funct_29_703
PubMedSearch : Jaques_2011_Cell.Biochem.Funct_29_703
PubMedID: 21932293

Title : Pre-treatment with ebselen and vitamin E modulate acetylcholinesterase activity: interaction with demyelinating agents - Mazzanti_2009_Int.J.Dev.Neurosci_27_73
Author(s) : Mazzanti CM , Spanevello R , Ahmed M , Pereira LB , Goncalves JF , Correa M , Schmatz R , Stefanello N , Leal DB , Mazzanti A , Ramos AT , Martins TB , Danesi CC , Graca DL , Morsch VM , Schetinger MR
Ref : Int J Developmental Neuroscience , 27 :73 , 2009
Abstract : The ethidium bromide (EB) demyelinating model was associated with vitamin E (Vit E) and ebselen (Ebs) treatment to evaluate acetylcholinesterase (AChE) activity in the striatum (ST), hippocampus (HP), cerebral cortex (CC) and erythrocytes. Rats were divided into seven groups: I-Control (saline), II-(canola); III-(Ebs), IV-(Vit E); V-(EB); VI-(EB+Ebs) and VII-(EB+Vit E). At 3 days after the EB injection, AChE activity in the CC and HC was significantly reduced in groups III, IV, V, VI and VII (p<0.05) and in the ST it was reduced in groups III and V (p<0.05) when compared to the control group. At 21 days after the EB injection, AChE activity in the CC was significantly reduced in groups III, IV and V, while in groups VI and VII a significant increase was observed when compared to the control group. In the HC and ST, AChE activity was significantly reduced in groups V, VI and VII when compared to the control group (p<0.05). In the erythrocytes, at 3 days after the EB injection, AChE activity was significantly reduced in groups III, IV, V, VI and VII and at 21 days there was a significant reduction only in groups VI and VII (p<0.05) when compared to the control group. In conclusion, this study demonstrated that Ebs and Vit E interfere with the cholinergic neurotransmission by altering AChE activity in the different brain regions and in the erythrocytes. Furthermore, treatment with Vit E and Ebs protected against the demyelination lesion caused by EB. In this context, we can suggest that ebselen and Vit E should be considered potential therapeutics and scientific tools to be investigated in brain disorders associated with demyelinating events.
ESTHER : Mazzanti_2009_Int.J.Dev.Neurosci_27_73
PubMedSearch : Mazzanti_2009_Int.J.Dev.Neurosci_27_73
PubMedID: 18930802