Mo J

References (10)

Title : Termiticidal, biochemical, and morpho-histological effects of botanical based nanoemulsion against a subterranean termite, Odontotermes Formosanus Shiraki - Nasser_2023_Front.Plant.Sci_14_1292272
Author(s) : Nasser R , Ibrahim E , Fouad H , Ahmad F , Li W , Zhou Q , Yu T , Chidwala N , Mo J
Ref : Front Plant Sci , 14 :1292272 , 2023
Abstract : Recently, the use of nanopesticides has shown significant efficacy in the control of many pests. However, the effect of nanopesticides, especially nanoemulsions, on suppressing termites, Odontotermes formosanus (Shiraki, 1909) (O. formosanus), has not been studied yet. Therefore, this study aimed to produce nanoemulsions of the essential oils of eucalyptus (Eucalyptus globulus Labill; E-EO) and nutmeg (Myristica fragrans Houtt; N-EO) to suppress O. formosanus. The analysis of eucalyptus nanoemulsion (E-NE) and nutmeg nanoemulsion (N-NE) was confirmed by using UV-Vis, dynamic light scattering, zeta potential, transmission electron microscopy, scanning electron microscopy, and energy dispersive spectroscopy. In addition, chemical analysis by Gas Chromatography with a mass spectrometer (GC-MS) exhibited the major constituents of E-NE and N-NE. The principal chemical components of E-NE included D-limonene, eucalyptol, 1,5-cyclooctadiene,3,4-dimethyl, benzene, and 1-methyl-3-(1 methylethyl)-, while the main constituents in N-NE were cyclohexane,1-methylene-4-(1 methylethenyl)-, eucalyptol, and L-. alpha. -terpineol. The mortality rates were 100% and 99.53%, respectively, after 24 hours of treatment with a concentration of 140 mg/mL, compared to 23.43% and 43.55%, respectively, from E-EO and N-EO treatment. These results refer to the essential oils' nanoemulsion as far more effective than the essential oils themselves. Furthermore, the effects of E-NE and N-NE on detoxification enzymes such as acetylcholinesterase, carboxylesterase, acid and alkaline phosphatase were investigated, as well as total protein concentrations, and the results have been found to be significantly increasing or decreasing in comparison with control. Besides, histological and morphological alterations found post exposure to E-NE and N-NE were shown. Overall, the results from this study clearly indicate that the nanopesticide-formulated nanoemulsions may have great potential to be used as novel, environmentally safe insecticides for controlling O. formosanus.
ESTHER : Nasser_2023_Front.Plant.Sci_14_1292272
PubMedSearch : Nasser_2023_Front.Plant.Sci_14_1292272
PubMedID: 38259939

Title : Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish - Wan_2022_Environ.Epigenet_8_dvac013
Author(s) : Wan T , Au DW , Mo J , Chen L , Cheung KM , Kong RY , Seemann F
Ref : Environ Epigenet , 8 :dvac013 , 2022
Abstract : Previous studies have revealed that DNA methylation changes could serve as potential genomic markers for environmental benzo[a]pyrene (BaP) exposure and intergenerational inheritance of various physiological impairments (e.g. obesity and reproductive pathologies). As a typical aromatic hydrocarbon pollutant, direct BaP exposure has been shown to induce neurotoxicity. To unravel the inheritance mechanisms of the BaP-induced bone phenotype in freshwater medaka, we conducted whole-genome bisulfite sequencing of F1 sperm and identified 776 differentially methylated genes (DMGs). Ingenuity pathway analysis revealed that DMGs were significantly enriched in pathways associated with neuronal development and function. Therefore, it was hypothesized that parental BaP exposure (1 microg/l, 21 days) causes offspring neurotoxicity. Furthermore, the possibility for sperm methylation as an indicator for a neurotoxic phenotype was investigated. The F0 adult brains and F1 larvae were analyzed for BaP-induced direct and inherited toxicity. Acetylcholinesterase activity was significantly reduced in the larvae, together with decreased swimming velocity. Molecular analysis revealed that the marker genes associated with neuron development and growth (alpha1-tubulin, mbp, syn2a, shh, and gap43) as well as brain development (dlx2, otx2, and krox-20) were universally downregulated in the F1 larvae (3 days post-hatching). While parental BaP exposure at an environmentally relevant concentration could induce neurotoxicity in the developing larvae, the brain function of the exposed F0 adults was unaffected. This indicates that developmental neurotoxicity in larvae may result from impaired neuronal development and differentiation, causing delayed brain growth. The present study demonstrates that the possible adverse health effects of BaP in the environment are more extensive than currently understood. Thus, the possibility of multigenerational BaP toxicity should be included in environmental risk assessments.
ESTHER : Wan_2022_Environ.Epigenet_8_dvac013
PubMedSearch : Wan_2022_Environ.Epigenet_8_dvac013
PubMedID: 35769199

Title : Design, synthesis, in vitro and in vivo evaluation of benzylpiperidine-linked 1,3-dimethylbenzimidazolinones as cholinesterase inhibitors against Alzheimer's disease - Mo_2020_J.Enzyme.Inhib.Med.Chem_35_330
Author(s) : Mo J , Chen T , Yang H , Guo Y , Li Q , Qiao Y , Lin H , Feng F , Liu W , Chen Y , Liu Z , Sun H
Ref : J Enzyme Inhib Med Chem , 35 :330 , 2020
Abstract : Cholinesterase inhibitor plays an important role in the treatment of patients with Alzheimer's disease (AD). Herein, we report the medicinal chemistry efforts leading to a new series of 1,3-dimethylbenzimidazolinone derivatives. Among the synthesised compounds, 15b and 15j showed submicromolar IC50 values (15b, eeAChE IC50 = 0.39 +/- 0.11 microM; 15j, eqBChE IC50 = 0.16 +/- 0.04 microM) towards acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Kinetic and molecular modelling studies revealed that 15b and 15j act in a competitive manner. 15b and 15j showed neuroprotective effect against H2O2-induced oxidative damage on PC12 cells. This effect was further supported by their antioxidant activity determined in a DPPH assay in vitro. Morris water maze test confirmed the memory amelioration effect of the two compounds in a scopolamine-induced mouse model. Moreover, the hepatotoxicity of 15b and 15j was lower than tacrine. In summary, these data suggest 15b and 15j are promising multifunctional agents against AD.
ESTHER : Mo_2020_J.Enzyme.Inhib.Med.Chem_35_330
PubMedSearch : Mo_2020_J.Enzyme.Inhib.Med.Chem_35_330
PubMedID: 31856607

Title : Functional analysis of the GbDWARF14 gene associated with branching development in cotton - Wang_2019_PeerJ_7_e6901
Author(s) : Wang P , Zhang S , Qiao J , Sun Q , Shi Q , Cai C , Mo J , Chu Z , Yuan Y , Du X , Miao Y , Zhang X , Cai Y
Ref : PeerJ , 7 :e6901 , 2019
Abstract : Plant architecture, including branching pattern, is an important agronomic trait of cotton crops. In recent years, strigolactones (SLs) have been considered important plant hormones that regulate branch development. In some species such as Arabidopsis, DWARF14 is an unconventional receptor that plays an important role in the SL signaling pathway. However, studies on SL receptors in cotton are still lacking. Here, we cloned and analysed the structure of the GbD14 gene in Gossypium barbadense and found that it contains the domains necessary for a SL receptor. The GbD14 gene was expressed primarily in the roots, leaves and vascular bundles, and the GbD14 protein was determined via GFP to localize to the cytoplasm and nucleus. Gene expression analysis revealed that the GbD14 gene not only responded to SL signals but also was differentially expressed between cotton plants whose types of branching differed. In particular, GbD14 was expressed mainly in the axillary buds of normal-branching cotton, while it was expressed the most in the leaves of nulliplex-branch cotton. In cotton, the GbD14 gene can be induced by SL and other plant hormones, such as indoleacetic acid, abscisic acid, and jasmonic acid. Compared with wild-type Arabidopsis, GbD14-overexpressing Arabidopsis responded more rapidly to SL signals. Moreover, we also found that GbD14 can rescue the multi-branched phenotype of Arabidopsis Atd14 mutants. Our results indicate that the function of GbD14 is similar to that of AtD14, and GbD14 may be a receptor for SL in cotton and involved in regulating branch development. This research provides a theoretical basis for a profound understanding of the molecular mechanism of branch development and ideal plant architecture for cotton breeding improvements.
ESTHER : Wang_2019_PeerJ_7_e6901
PubMedSearch : Wang_2019_PeerJ_7_e6901
PubMedID: 31143538

Title : Design, synthesis, biological evaluation, and molecular modeling studies of quinoline-ferulic acid hybrids as cholinesterase inhibitors - Mo_2019_Bioorg.Chem_93_103310
Author(s) : Mo J , Yang H , Chen T , Li Q , Lin H , Feng F , Liu W , Qu W , Guo Q , Chi H , Chen Y , Sun H
Ref : Bioorg Chem , 93 :103310 , 2019
Abstract : A series of quinoline-ferulic acid hybrids has been designed, synthesized, and evaluated as cholinesterase inhibitors. Most of the compounds showed good inhibitory activities toward both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Among them, 10f was found to be the most potent inhibitor against AChE (IC50=0.62+/-0.17mum), and 14 was the most potent inhibitor against BChE (IC50=0.10+/-0.01mum). Representative compounds, such as 10f and 12g, act in a competitive manner when they inhibit AChE or BChE. Molecular docking and dynamic simulation revealed that the synthesized compounds bind to the target by simultaneously interacting with the catalytic active site (CAS) and the peripheral anionic site (PAS) of both AChE and BChE. The U-shaped confirmation was preferred when 12g bound to BChE, which was different from the linear conformation of 10f bound to AChE. Cell-based assays have confirmed the moderate neuroprotective effects of compounds 10f and 12g against H2O2-induced oxidative damage towards PC12 cells. Moreover, the hepatotoxicity of 12g was lower than that of tacrine, indicating its potential safety as an anti-Alzheimer's agent. In summary, we report a new chemotype of multifunctional hybrid, which may be further modified to develop new anti-Alzheimer's agents.
ESTHER : Mo_2019_Bioorg.Chem_93_103310
PubMedSearch : Mo_2019_Bioorg.Chem_93_103310
PubMedID: 31586704

Title : Synthesis, pharmacology and molecular docking on multifunctional tacrine-ferulic acid hybrids as cholinesterase inhibitors against Alzheimer's disease - Zhu_2018_J.Enzyme.Inhib.Med.Chem_33_496
Author(s) : Zhu J , Yang H , Chen Y , Lin H , Li Q , Mo J , Bian Y , Pei Y , Sun H
Ref : J Enzyme Inhib Med Chem , 33 :496 , 2018
Abstract : The cholinergic hypothesis has long been a "polar star" in drug discovery for Alzheimer's disease (AD), resulting in many small molecules and biological drug candidates. Most of the drugs marketed for AD are cholinergic. Herein, we report our efforts in the discovery of cholinesterases inhibitors (ChEIs) as multi-target-directed ligands. A series of tacrine-ferulic acid hybrids have been designed and synthesised. All these compounds showed potent acetyl-(AChE) and butyryl cholinesterase(BuChE) inhibition. Among them, the optimal compound 10g, was the most potent inhibitor against AChE (electrophorus electricus (eeAChE) half maximal inhibitory concentration (IC50) = 37.02 nM), it was also a strong inhibitor against BuChE (equine serum (eqBuChE) IC50 = 101.40 nM). Besides, it inhibited amyloid beta-protein self-aggregation by 65.49% at 25 muM. In subsequent in vivo scopolamine-induced AD models, compound 10g obviously ameliorated the cognition impairment and showed preliminary safety in hepatotoxicity evaluation. These data suggest compound 10g as a promising multifunctional agent in the drug discovery process against AD.
ESTHER : Zhu_2018_J.Enzyme.Inhib.Med.Chem_33_496
PubMedSearch : Zhu_2018_J.Enzyme.Inhib.Med.Chem_33_496
PubMedID: 29405075

Title : Synthesis and bioevaluation of new tacrine-cinnamic acid hybrids as cholinesterase inhibitors against Alzheimer's disease - Chen_2018_J.Enzyme.Inhib.Med.Chem_33_290
Author(s) : Chen Y , Zhu J , Mo J , Yang H , Jiang X , Lin H , Gu K , Pei Y , Wu L , Tan R , Hou J , Chen J , Lv Y , Bian Y , Sun H
Ref : J Enzyme Inhib Med Chem , 33 :290 , 2018
Abstract : Small molecule cholinesterases inhibitor (ChEI) provides an effective therapeutic strategy to treat Alzheimer's disease (AD). Currently, the discovery of new ChEI with multi-target effect is still of great importance. Herein, we report the synthesis, structure-activity relationship study and biological evaluation of a series of tacrine-cinnamic acid hybrids as new ChEIs. All target compounds are evaluated for their in vitro cholinesterase inhibitory activities. The representatives which show potent activity on cholinesterase, are evaluated for the amyloid beta-protein self-aggregation inhibition and in vivo assays. The optimal compound 19, 27, and 30 (human AChE IC50 = 10.2 +/- 1.2, 16.5 +/- 1.7, and 15.3 +/- 1.8 nM, respectively) show good performance in ameliorating the scopolamine-induced cognition impairment and preliminary safety in hepatotoxicity evaluation. These compounds deserve further evaluation for the development of new therapeutic agents against AD.
ESTHER : Chen_2018_J.Enzyme.Inhib.Med.Chem_33_290
PubMedSearch : Chen_2018_J.Enzyme.Inhib.Med.Chem_33_290
PubMedID: 29278947

Title : Developmental expression and subcellular distribution of synaptotagmin 11 in rat hippocampus - Yeo_2012_Neurosci_225_35
Author(s) : Yeo H , Kim HW , Mo J , Lee D , Han S , Hong S , Koh MJ , Sun W , Choi S , Rhyu IJ , Kim H , Lee HW
Ref : Neuroscience , 225 :35 , 2012
Abstract : Synaptotagmins are required for Ca(2+)-dependent membrane-trafficking in either neuronal synaptic vesicles or cellular membranes. Previous reports suggested that the synaptotagmin 11 (syt11) gene is involved in the development of schizophrenia based on the genomic analysis of patients. Parkin protein binds to the C2 domains of Syt11 which leads to the polyubiquitination of Syt11. However, where and how Syt11 performs its role in the brain is largely unknown. Here, we report that Syt11 is expressed mainly in the brain. In addition, exogenously expressed Syt11 in HEK293 cells can form higher molecular weight complex via its transmembrane domain. Also, Syt11 is targeted to both dendrite and axon compartments. Immunocytochemistry showed that Syt11 is juxtaposed to postsynaptic markers in both excitatory and inhibitory synapses. Both neuroligin 1 and 2, which are postsynaptic cell adhesion molecules and differentially induce excitatory and inhibitory presynapses, respectively, recruit Syt11 in neuron coculture. Immunogold electron microscopy analysis revealed that Syt11 exists mainly in presynaptic neurotransmitter vesicles and plasma membrane, and rarely in postsynaptic sites. These results suggest that Syt11 may contribute to the regulation of neurotransmitter release in the excitatory and inhibitory presynapses, and postsynapse-targeted membrane trafficking in dendrites.
ESTHER : Yeo_2012_Neurosci_225_35
PubMedSearch : Yeo_2012_Neurosci_225_35
PubMedID: 22960622

Title : Numerical chromosomal changes and risk of development of myelodysplastic syndrome--acute myeloid leukemia in patients with Fanconi anemia - Mehta_2010_Cancer.Genet.Cytogenet_203_180
Author(s) : Mehta PA , Harris RE , Davies SM , Kim MO , Mueller R , Lampkin B , Mo J , Myers K , Smolarek TA
Ref : Cancer Genet Cytogenet , 203 :180 , 2010
Abstract : Fanconi Anemia (FA) is an inherited bone marrow failure syndrome characterized by congenital abnormalities, progressive marrow failure and predisposition to myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and solid tumors. The most common acquired chromosomal aberrations in FA patients are trisomy of 1q and monosomy of chromosome 7; the latter is known to be associated with poor prognosis. A few reports also suggest that gains of 3q are associated with progression to MDS-AML and overall poor prognosis. It is not uncommon for patients with Fanconi anemia to have easily detectable (oligoclonal) chromosomal alterations in their still normal (nonmalignant) marrow, which makes it even more challenging to determine the import of such alterations. We conducted a retrospective longitudinal analysis of fluorescent in situ hybridization (FISH) analysis for gains in 1q and 3q and for monosomy 7 and 7q deletions on 212 bone marrow samples from 77 children with FA treated at our institution between 1987 and 2007. Given the baseline increased chromosomal instability and defective DNA repair in patients with FA, which leads to unbalanced chromosomal aberrations such as deletions, insertions, and translocations, for the purpose of this analysis an abnormal clone was defined as >/=10% abnormal cells. Chromosome 3 and 7 aberrations were associated with increased risk of developing MDS-AML (P = 0.019 and P < 0.001 respectively), although the significance of chromosome 3 aberrations disappeared when different observation times were accounted for. Gain of 1q alone did not predict development of MDS-AML. In conclusion, children with FA should be followed closely with FISH analyses, because some of the clonal chromosomal abnormalities may be early indicators of progression toward MDS-AML and thus also of the need for hematopoietic stem cell transplantation.
ESTHER : Mehta_2010_Cancer.Genet.Cytogenet_203_180
PubMedSearch : Mehta_2010_Cancer.Genet.Cytogenet_203_180
PubMedID: 21156231

Title : Construction and validation of an insecticide resistance-associated DNA microarray - Ding_2007_J.Pestic.Sci_32_32
Author(s) : Ding M , Gao Q , Mo J , Cheng J
Ref : Journal of Pesticide Science , 32 :32 , 2007
Abstract : A microarray containing PCR products from a large number of candidate genes in insecticide resistance was constructed. A total of 646 genes putatively involved in insecticide resistance and positive control genes were obtained from six insect species, Apis cerana, Blattella germanica, Spodoptera exigua, Musca domestica, Nilaparrata lugens and Culex pipiens. These genes represent cytochrome P450, ion channel, protective enzyme and other genes, which may play important roles in insecticide resistance. The optical microarray printing concentration (250 ng/l) was determined using gradient concentration hybridization assay. The DNA microarray was then constructed with all target genes. A PCR probe composed of 18 target genes was prepared to validate the hybridization specificity of the microarray. The positive rate of this validation test was 83.3%. In addition, analysis of differential gene expression between susceptible and multi-resistant (cypermethrin and malathion) strains of M. domestica showed that 17 genes were over-expressed in the resistant strain. The results imply that cytochrome P450 genes, especially the genes of CYP4 and 6 families, play important roles in the resistance of M. domestica to both insecticides. The 17 genes validated by using qRT-PCR were found to have a similar tendency, mostly compared with using a microarray, indicating that this microarray is suitable for studying insecticide resistance.
ESTHER : Ding_2007_J.Pestic.Sci_32_32
PubMedSearch : Ding_2007_J.Pestic.Sci_32_32