Morrison M

References (11)

Title : Draft Genome Sequence of Ralstonia pickettii AU12-08, Isolated from an Intravascular Catheter in Australia - Zhang_2014_Genome.Announc_2_e00027
Author(s) : Zhang L , Morrison M , Rickard CM
Ref : Genome Announc , 2 : , 2014
Abstract : Ralstonia pickettii is a nonfermenting Gram-negative bacillus that creates a significant problem in clinical settings, as it is a widespread cause of nosocomial infections. Here, we report the draft genome sequence of R. pickettii AU12-08, isolated from an intravascular catheter tip.
ESTHER : Zhang_2014_Genome.Announc_2_e00027
PubMedSearch : Zhang_2014_Genome.Announc_2_e00027
PubMedID: 24503988
Gene_locus related to this paper: 9rals-s9rw78 , 9rals-s9tfx3 , 9rals-s9teu7

Title : Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach - Gagen_2014_BMC.Microbiol_14_314
Author(s) : Gagen EJ , Wang J , Padmanabha J , Liu J , de Carvalho IP , Webb RI , Al Jassim R , Morrison M , Denman SE , McSweeney CS
Ref : BMC Microbiol , 14 :314 , 2014
Abstract : BACKGROUND: Forestomach fermentation in Australian marsupials such as wallabies and kangaroos, though analogous to rumen fermentation, results in lower methane emissions. Insights into hydrogenotrophy in these systems could help in devising strategies to reduce ruminal methanogenesis. Reductive acetogenesis may be a significant hydrogen sink in these systems and previous molecular analyses have revealed a novel diversity of putative acetogens in the tammar wallaby forestomach.
RESULTS: Methanogen-inhibited enrichment cultures prepared from tammar wallaby forestomach contents consumed hydrogen and produced primarily acetate. Functional gene (formyltetrahydrofolate synthetase and acetyl-CoA synthase) analyses revealed a restricted diversity of Clostridiales species as the putative acetogens in the cultures. A new acetogen (growth on H2/CO2 with acetate as primary end product) designated isolate TWA4, was obtained from the cultures. Isolate TWA4 classified within the Lachnospiraceae and demonstrated >97% rrs identity to previously isolated kangaroo acetogens. Isolate TWA4 was a potent hydrogenotroph and demonstrated excellent mixotrophic growth (concomitant consumption of hydrogen during heterotrophic growth) with glycerol. Mixotrophic growth of isolate TWA4 on glycerol resulted in increased cell densities and acetate production compared to autotrophic growth. Co-cultures with an autotrophic methanogen Methanobrevibacter smithii revealed that isolate TWA4 performed reductive acetogenesis under high hydrogen concentration (>5 mM), but not at low concentrations. Under heterotrophic growth conditions, isolate TWA4 did not significantly stimulate methanogenesis in a co-culture with M. smithii contrary to the expectation for organisms growing fermentatively.
CONCLUSIONS: The unique properties of tammar wallaby acetogens might be contributing factors to reduced methanogen numbers and methane emissions from tammar wallaby forestomach fermentation, compared to ruminal fermentation. The macropod forestomach may be a useful source of acetogens for future strategies to reduce methane emissions from ruminants, particularly if these strategies also include some level of methane suppression and/or acetogen stimulation, for example by harnessing mixotrophic growth capabilities.
ESTHER : Gagen_2014_BMC.Microbiol_14_314
PubMedSearch : Gagen_2014_BMC.Microbiol_14_314
PubMedID: 25495654
Gene_locus related to this paper: 9firm-a0a0d0sh39

Title : Draft Genome Sequence of Enterococcus faecalis PC1.1, a Candidate Probiotic Strain Isolated from Human Feces - O Cuv_2013_Genome.Announc_1_e00160
Author(s) : O Cuv P , Klaassens ES , Smith WJ , Mondot S , Durkin AS , Harkins DM , Foster L , McCorrison J , Torralba M , Nelson KE , Morrison M
Ref : Genome Announc , 1 : e00160 , 2013
Abstract : Enterococcus faecalis is commonly isolated from the gastrointestinal tract of healthy infants and adults, where it contributes to host health and well-being. We describe here the draft genome sequence of E. faecalis PC1.1, a candidate probiotic strain isolated from human feces.
ESTHER : O Cuv_2013_Genome.Announc_1_e00160
PubMedSearch : O Cuv_2013_Genome.Announc_1_e00160
PubMedID: 23469340
Gene_locus related to this paper: entfl-e2z7d4

Title : Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows - Wang_2013_PLoS.One_8_e78507
Author(s) : Wang L , Hatem A , Catalyurek UV , Morrison M , Yu Z
Ref : PLoS ONE , 8 :e78507 , 2013
Abstract : The ruminal microbial community is a unique source of enzymes that underpin the conversion of cellulosic biomass. In this study, the microbial consortia adherent on solid digesta in the rumen of Jersey cattle were subjected to an activity-based metagenomic study to explore the genetic diversity of carbohydrolytic enzymes in Jersey cows, with a particular focus on cellulases and xylanases. Pyrosequencing and bioinformatic analyses of 120 carbohydrate-active fosmids identified genes encoding 575 putative Carbohydrate-Active Enzymes (CAZymes) and proteins putatively related to transcriptional regulation, transporters, and signal transduction coupled with polysaccharide degradation and metabolism. Most of these genes shared little similarity to sequences archived in databases. Genes that were predicted to encode glycoside hydrolases (GH) involved in xylan and cellulose hydrolysis (e.g., GH3, 5, 9, 10, 39 and 43) were well represented. A new subfamily (S-8) of GH5 was identified from contigs assigned to Firmicutes. These subfamilies of GH5 proteins also showed significant phylum-dependent distribution. A number of polysaccharide utilization loci (PULs) were found, and two of them contained genes encoding Sus-like proteins and cellulases that have not been reported in previous metagenomic studies of samples from the rumens of cows or other herbivores. Comparison with the large metagenomic datasets previously reported of other ruminant species (or cattle breeds) and wallabies showed that the rumen microbiome of Jersey cows might contain differing CAZymes. Future studies are needed to further explore how host genetics and diets affect the diversity and distribution of CAZymes and utilization of plant cell wall materials.
ESTHER : Wang_2013_PLoS.One_8_e78507
PubMedSearch : Wang_2013_PLoS.One_8_e78507
PubMedID: 24223817
Gene_locus related to this paper: 9bact-w0fkk5 , 9bact-w0fjm8 , 9bact-w0fh15 , 9bact-w0fgx1 , 9bact-w0fls2

Title : Genome Sequence of Stenotrophomonas maltophilia Strain AU12-09, Isolated from an Intravascular Catheter - Zhang_2013_Genome.Announc_1_e00195
Author(s) : Zhang L , Morrison M , P OC , Evans P , Rickard CM
Ref : Genome Announc , 1 :e00195 , 2013
Abstract : Stenotrophomonas maltophilia is an opportunistic nosocomial pathogen that is characterized by its high-level intrinsic resistance to a variety of antibiotics and its ability to form biofilms. Here, we report the draft genome sequence of Stenotrophomonas maltophilia AU12-09, isolated from an intravascular catheter tip.
ESTHER : Zhang_2013_Genome.Announc_1_e00195
PubMedSearch : Zhang_2013_Genome.Announc_1_e00195
PubMedID: 23640378
Gene_locus related to this paper: stema-t5kff6

Title : Draft genome sequence of Treponema sp. strain JC4, a novel spirochete isolated from the bovine rumen - Rosewarne_2012_J.Bacteriol_194_4130
Author(s) : Rosewarne CP , Cheung JL , Smith WJ , Evans PN , Tomkins NW , Denman SE , P OC , Morrison M
Ref : Journal of Bacteriology , 194 :4130 , 2012
Abstract : Morphologically and biochemically diverse members of the Treponema genus are present in the gastrointestinal tract of ruminants, yet very little is understood about their functional importance to this microbiome. Here we describe the annotated draft genome sequence of Treponema sp. strain JC4, a novel spirochete isolated from a bovine rumen sample.
ESTHER : Rosewarne_2012_J.Bacteriol_194_4130
PubMedSearch : Rosewarne_2012_J.Bacteriol_194_4130
PubMedID: 22815447
Gene_locus related to this paper: 9spio-i0x819 , 9spio-i0xbf6

Title : Genome sequence of Staphylococcus epidermidis strain AU12-03, isolated from an intravascular catheter - Zhang_2012_J.Bacteriol_194_6639
Author(s) : Zhang L , Morrison M , P OC , Evans P , Rickard CM
Ref : Journal of Bacteriology , 194 :6639 , 2012
Abstract : In recent years, Staphylococcus epidermidis has become a major nosocomial pathogen and the most common cause of intravascular catheter-related bacteremia, which can increase morbidity and mortality and significantly affect patient recovery. We report a draft genome sequence of Staphylococcus epidermidis AU12-03, isolated from an intravascular catheter tip.
ESTHER : Zhang_2012_J.Bacteriol_194_6639
PubMedSearch : Zhang_2012_J.Bacteriol_194_6639
PubMedID: 23144393
Gene_locus related to this paper: staep-SERP2035

Title : Draft genome sequence of Bacteroides vulgatus PC510, a strain isolated from human feces - Cuiv_2011_J.Bacteriol_193_4025
Author(s) : Cuiv PO , Klaassens ES , Durkin AS , Harkins DM , Foster L , McCorrison J , Torralba M , Nelson KE , Morrison M
Ref : Journal of Bacteriology , 193 :4025 , 2011
Abstract : Although Bacteroides vulgatus is one of the most prevalent microorganisms in the human gastrointestinal tract little is known about the genetic potential of this species Here we describe the annotated draft genome sequence of B vulgatus PC510 isolated from human feces.
ESTHER : Cuiv_2011_J.Bacteriol_193_4025
PubMedSearch : Cuiv_2011_J.Bacteriol_193_4025
PubMedID: 21622758
Gene_locus related to this paper: 9bace-b6w170 , bacv8-a6kwf6 , bacv8-a6kzc1 , bacv8-a6kze8 , bacv8-a6l1d0 , bacv8-a6l1u9 , bacv8-a6l018 , bacv8-a6l715 , bacvu-d4vbw6

Title : Draft genome sequence of Turicibacter sanguinis PC909, isolated from human feces - Cuiv_2011_J.Bacteriol_193_1288
Author(s) : Cuiv PO , Klaassens ES , Durkin AS , Harkins DM , Foster L , McCorrison J , Torralba M , Nelson KE , Morrison M
Ref : Journal of Bacteriology , 193 :1288 , 2011
Abstract : While the microbiota resident in the human gut is now known to provide a range of functions relevant to host health, many of the microbial members of the community have not yet been cultured or are represented by a limited number of isolates. We describe here the draft genome sequence of Turicibacter sanguinis PC909, isolated from a pooled healthy human fecal sample as part of the Australian Human Gut Microbiome Project.
ESTHER : Cuiv_2011_J.Bacteriol_193_1288
PubMedSearch : Cuiv_2011_J.Bacteriol_193_1288
PubMedID: 21183674
Gene_locus related to this paper: 9firm-d4w7y6 , 9firm-d4w7y7 , 9firm-f0hgc7 , 9firm-d4w6y8 , 9firm-d4w5a4

Title : Biochemical characterization and relative expression levels of multiple carbohydrate esterases of the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate - Kabel_2011_Appl.Environ.Microbiol_77_5671
Author(s) : Kabel MA , Yeoman CJ , Han Y , Dodd D , Abbas CA , de Bont JA , Morrison M , Cann IK , Mackie RI
Ref : Applied Environmental Microbiology , 77 :5671 , 2011
Abstract : We measured expression and used biochemical characterization of multiple carbohydrate esterases by the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate to gain insight into the carbohydrate esterase activities of this hemicellulolytic rumen bacterium. The P. ruminicola 23 genome contains 16 genes predicted to encode carbohydrate esterase activity, and based on microarray data, four of these were upregulated >2-fold at the transcriptional level during growth on an ester-enriched oligosaccharide (XOS(FA,Ac)) from corn relative to a nonesterified fraction of corn oligosaccharides (AXOS). Four of the 16 esterases (Xyn10D-Fae1A, Axe1-6A, AxeA1, and Axe7A), including the two most highly induced esterases (Xyn10D-Fae1A and Axe1-6A), were heterologously expressed in Escherichia coli, purified, and biochemically characterized. All four enzymes showed the highest activity at physiologically relevant pH (6 to 7) and temperature (30 to 40 degrees C) ranges. The P. ruminicola 23 Xyn10D-Fae1A (a carbohydrate esterase [CE] family 1 enzyme) released ferulic acid from methylferulate, wheat bran, corn fiber, and XOS(FA,Ac), a corn fiber-derived substrate enriched in O-acetyl and ferulic acid esters, but exhibited negligible activity on sugar acetates. As expected, the P. ruminicola Axe1-6A enzyme, which was predicted to possess two distinct esterase family domains (CE1 and CE6), released ferulic acid from the same substrates as Xyn10D-Fae1 and was also able to cleave O-acetyl ester bonds from various acetylated oligosaccharides (AcXOS). The P. ruminicola 23 AxeA1, which is not assigned to a CE family, and Axe7A (CE7) were found to be acetyl esterases that had activity toward a broad range of mostly nonpolymeric acetylated substrates along with AcXOS. All enzymes were inhibited by the proximal location of other side groups like 4-O-methylglucuronic acid, ferulic acid, or acetyl groups. The unique diversity of carbohydrate esterases in P. ruminicola 23 likely gives it the ability to hydrolyze substituents on the xylan backbone and enhances its capacity to efficiently degrade hemicellulose.
ESTHER : Kabel_2011_Appl.Environ.Microbiol_77_5671
PubMedSearch : Kabel_2011_Appl.Environ.Microbiol_77_5671
PubMedID: 21742923
Gene_locus related to this paper: prer2-axea1 , prer2-axfa

Title : Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii: insights into their environmental niche - Purushe_2010_Microb.Ecol_60_721
Author(s) : Purushe J , Fouts DE , Morrison M , White BA , Mackie RI , Coutinho PM , Henrissat B , Nelson KE
Ref : Microb Ecol , 60 :721 , 2010
Abstract : The Prevotellas comprise a diverse group of bacteria that has received surprisingly limited attention at the whole genome-sequencing level. In this communication, we present the comparative analysis of the genomes of Prevotella ruminicola 23 (GenBank: CP002006) and Prevotella bryantii B(1)4 (GenBank: ADWO00000000), two gastrointestinal isolates. Both P. ruminicola and P. bryantii have acquired an extensive repertoire of glycoside hydrolases that are targeted towards non-cellulosic polysaccharides, especially GH43 bifunctional enzymes. Our analysis demonstrates the diversity of this genus. The results from these analyses highlight their role in the gastrointestinal tract, and provide a template for additional work on genetic characterization of these species.
ESTHER : Purushe_2010_Microb.Ecol_60_721
PubMedSearch : Purushe_2010_Microb.Ecol_60_721
PubMedID: 20585943
Gene_locus related to this paper: prebr-d8dt96 , prebr-d8du06 , prebr-d8dv28 , prebr-d8dwg8 , prebr-d8dy06 , prer2-d5esm6 , prebr-d8dul5 , prebr-d8dxa2 , prebr-d8dw03 , prer2-d5erq4 , prer2-d5ewz1 , prebr-d8dv01 , prer2-axea1 , prer2-axfa