Nagata N

References (5)

Title : Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus - Iwata-Yoshikawa_2019_J.Virol_93_
Author(s) : Iwata-Yoshikawa N , Okamura T , Shimizu Y , Kotani O , Sato H , Sekimukai H , Fukushi S , Suzuki T , Sato Y , Takeda M , Tashiro M , Hasegawa H , Nagata N
Ref : J Virol , 93 : , 2019
Abstract : Middle East respiratory syndrome coronavirus (MERS-CoV) infection can manifest as a mild illness, acute respiratory distress, organ failure, or death. Several animal models have been established to study disease pathogenesis and to develop vaccines and therapeutic agents. Here, we developed transgenic (Tg) mice on a C57BL/6 background; these mice expressed human CD26/dipeptidyl peptidase 4 (hDPP4), a functional receptor for MERS-CoV, under the control of an endogenous hDPP4 promoter. We then characterized this mouse model of MERS-CoV. The expression profile of hDPP4 in these mice was almost equivalent to that in human tissues, including kidney and lung; however, hDPP4 was overexpressed in murine CD3-positive cells within peripheral blood and lymphoid tissues. Intranasal inoculation of young and adult Tg mice with MERS-CoV led to infection of the lower respiratory tract and pathological evidence of acute multifocal interstitial pneumonia within 7 days, with only transient loss of body weight. However, the immunopathology in young and adult Tg mice was different. On day 5 or 7 postinoculation, lungs of adult Tg mice contained higher levels of proinflammatory cytokines and chemokines associated with migration of macrophages. These results suggest that the immunopathology of MERS-CoV infection in the Tg mouse is age dependent. The mouse model described here will increase our understanding of disease pathogenesis and host mediators that protect against MERS-CoV infection.IMPORTANCE Middle East respiratory syndrome coronavirus (MERS-CoV) infections are endemic in the Middle East and a threat to public health worldwide. Rodents are not susceptible to the virus because they do not express functional receptors; therefore, we generated a new animal model of MERS-CoV infection based on transgenic mice expressing human DPP4 (hDPP4). The pattern of hDPP4 expression in this model was similar to that in human tissues (except lymphoid tissue). In addition, MERS-CoV was limited to the respiratory tract. Here, we focused on host factors involved in immunopathology in MERS-CoV infection and clarified differences in antiviral immune responses between young and adult transgenic mice. This new small-animal model could contribute to more in-depth study of the pathology of MERS-CoV infection and aid development of suitable treatments.
ESTHER : Iwata-Yoshikawa_2019_J.Virol_93_
PubMedSearch : Iwata-Yoshikawa_2019_J.Virol_93_
PubMedID: 30626685

Title : Temporal deterioration of neurological symptoms and increase of serum acetylcholine receptor antibody levels after thymectomy: a case report of a cat with myasthenia gravis - Nagata_2017_J.Vet.Med.Sci_78_1893
Author(s) : Nagata N , Miyoshi T , Otake Y , Suzuki H , Kagawa Y , Yamagami T , Irie M
Ref : J Vet Med Sci , 78 :1893 , 2017
Abstract : Neurological signs and serum acetylcholine receptor antibody (AChR-Ab) levels before and after thymectomy were monitored in a 6-year-old male cat with acquired Myasthenia Gravis (MG) as a paraneoplastic syndrome of thymoma. Soon after surgery, the neurological symptoms relapsed, and the cholinesterase inhibitor was administered to control them. The AChR-Ab levels increased postoperatively until 90 days after surgery. This is the first report on long term measurements of serum AChR-Ab levels in a cat with MG. Although thymectomy is valuable for the removal of thymoma, it may not resolve MG symptoms, neurological signs and serum AChR-Ab levels, without medication early after surgery. Also, this case report indicates that the AChR-Ab level might be a guide to detect a deterioration of MG symptoms.
ESTHER : Nagata_2017_J.Vet.Med.Sci_78_1893
PubMedSearch : Nagata_2017_J.Vet.Med.Sci_78_1893
PubMedID: 27593682

Title : Non Susceptibility of Neonatal and Adult Rats against the Middle East Respiratory Syndrome Coronavirus - Iwata-Yoshikawa_2016_Jpn.J.Infect.Dis_69_510
Author(s) : Iwata-Yoshikawa N , Fukushi S , Fukuma A , Suzuki T , Takeda M , Tashiro M , Hasegawa H , Nagata N
Ref : Jpn J Infect Dis , 69 :510 , 2016
Abstract : The present study examined the susceptibility of rats to the Middle East respiratory syndrome coronavirus (MERS-CoV) and determined whether this animal is a suitable model for MERS-CoV infection. Immunohistochemical analysis identified dipeptidyl peptidase 4 (DPP4), a known receptor for MERS-CoV on type I pneumocytes from infected rats. Whereas adult rats developed antibodies against MERS-CoV spike protein after intranasal inoculation, there was no evidence of viral replication in the lungs of adult, young, or neonatal rats after intranasal inoculation with MERS-CoV. In addition, human DPP4-expressing rat kidney fibroblasts, but not rat DPP4-expressing cells, were susceptible to MERS-CoV. Taken together, these results suggest that the rat is not a useful animal model for studying MERS-CoV infection.
ESTHER : Iwata-Yoshikawa_2016_Jpn.J.Infect.Dis_69_510
PubMedSearch : Iwata-Yoshikawa_2016_Jpn.J.Infect.Dis_69_510
PubMedID: 27000459

Title : Soluble Epoxide Hydrolase Deficiency or Inhibition Attenuates Diet-induced Endoplasmic Reticulum Stress in Liver and Adipose Tissue - Bettaieb_2013_J.Biol.Chem_288_14189
Author(s) : Bettaieb A , Nagata N , Aboubechara D , Chahed S , Morisseau C , Hammock BD , Haj FG
Ref : Journal of Biological Chemistry , 288 :14189 , 2013
Abstract : Soluble epoxide hydrolase (sEH) is a cytosolic enzyme whose inhibition has beneficial effects in cardiovascular, inflammatory, and metabolic diseases in murine models. Mice with targeted deletion or pharmacological inhibition of sEH exhibit improved insulin signaling in liver and adipose tissue. Herein, we assessed the role of sEH in regulating endoplasmic reticulum (ER) stress in liver and adipose tissue. We report that sEH expression was increased in the livers and adipose tissue of mice fed a high fat diet, the adipose tissue of overweight humans, and palmitate-treated cells. Importantly, sEH deficiency or inhibition in mice attenuated chronic high fat diet-induced ER stress in liver and adipose tissue. Similarly, pharmacological inhibition of sEH in HepG2 cells and 3T3-L1 adipocytes mitigated chemical-induced ER stress and activation of JNK, p38, and cell death. In addition, insulin signaling was enhanced in HepG2 cells treated with sEH substrates and attenuated in cells treated with sEH products. In summary, these findings demonstrate that sEH is a physiological modulator of ER stress and a potential target for mitigating complications associated with obesity.
ESTHER : Bettaieb_2013_J.Biol.Chem_288_14189
PubMedSearch : Bettaieb_2013_J.Biol.Chem_288_14189
PubMedID: 23576437

Title : Cytochemical and immunocytochemical demonstration of acetylcholinesterase of the prenatal rat lower limb - Umezu_1993_Arch.Hist.Cytol_56_217
Author(s) : Umezu Y , Nagata N , Doi Y , Furukawa H , Sagara T , Hayashida T , Ogata H , Fujimoto S
Ref : Archives of Histology & Cytology , 56 :217 , 1993
Abstract : Acetylcholinesterase (AChE) activities in the prenatal rat lower limb were investigated by both cytochemistry and immunocytochemistry. Results indicate that the epidermal cells show immunoreactions of AChE at a limited stage at prenatal day 15, and mesenchymal cells which are occasionally in contact with the basal lamina or with the adjacent myotubes begin to show AChE activities at prenatal day 17. Such AChE-positive mesenchymal cells, involved in the formation of the muscular tissues, have almost disappeared in the subepidermis by prenatal day 19. This suggests that AChE independent of the neuromuscular system may be involved in the mesenchymal cell differentiation especially in the inductive process during myogenesis.
ESTHER : Umezu_1993_Arch.Hist.Cytol_56_217
PubMedSearch : Umezu_1993_Arch.Hist.Cytol_56_217
PubMedID: 8373663