Nie Q

References (5)

Title : Analysis of changes in inter-cellular communications during Alzheimer's Disease pathogenesis reveals conserved changes in glutamatergic transmission in mice and humans - Bartas_2024_bioRxiv__
Author(s) : Bartas K , Hui M , Zhao W , Macchia D , Nie Q , Beier KT
Ref : Biorxiv , : , 2024
Abstract : Analysis of system-wide cellular communication changes in Alzheimer's disease (AD) has recently been enabled by single nucleus RNA sequencing (snRNA-seq) and new computational methods. Here, we combined these to analyze data from postmortem human tissue from the entorhinal cortex of AD patients and compared our findings to those from multiomic data from the 5xFAD amyloidogenic mouse model at two different time points. Using the cellular communication inference tool CellChat we found that disease-related changes were largely related to neuronal excitability as well as synaptic communication, with specific signaling pathways including BMP, EGF, and EPHA, and relatively poor conservation of glial-related changes during disease. Further analysis using the neuron-specific NeuronChat revealed changes relating to metabotropic glutamate receptors as well as neuronal adhesion molecules including neurexins and neuroligins. Our results that cellular processes relating to excitotoxicity are the best conserved between 5xFAD mice and AD suggest that excitotoxicity is the main common feature between pathogenesis in 5xFAD mice and AD patients.
ESTHER : Bartas_2024_bioRxiv__
PubMedSearch : Bartas_2024_bioRxiv__
PubMedID: 38746369

Title : Microbial-host-isozyme analyses reveal microbial DPP4 as a potential antidiabetic target - Wang_2023_Science_381_eadd5787
Author(s) : Wang K , Zhang Z , Hang J , Liu J , Guo F , Ding Y , Li M , Nie Q , Lin J , Zhuo Y , Sun L , Luo X , Zhong Q , Ye C , Yun C , Zhang Y , Wang J , Bao R , Pang Y , Wang G , Gonzalez FJ , Lei X , Qiao J , Jiang C
Ref : Science , 381 :eadd5787 , 2023
Abstract : A mechanistic understanding of how microbial proteins affect the host could yield deeper insights into gut microbiota-host cross-talk. We developed an enzyme activity-screening platform to investigate how gut microbiota-derived enzymes might influence host physiology. We discovered that dipeptidyl peptidase 4 (DPP4) is expressed by specific bacterial taxa of the microbiota. Microbial DPP4 was able to decrease the active glucagon like peptide-1 (GLP-1) and disrupt glucose metabolism in mice with a leaky gut. Furthermore, the current drugs targeting human DPP4, including sitagliptin, had little effect on microbial DPP4. Using high-throughput screening, we identified daurisoline-d4 (Dau-d4) as a selective microbial DPP4 inhibitor that improves glucose tolerance in diabetic mice.
ESTHER : Wang_2023_Science_381_eadd5787
PubMedSearch : Wang_2023_Science_381_eadd5787
PubMedID: 37535747
Gene_locus related to this paper: bactn-BT4193

Title : Effects of abhydrolase domain containing 5 gene (ABHD5) expression and variations on chicken fat metabolism - Ouyang_2016_Poult.Sci_95_99
Author(s) : Ouyang H , Liu Q , Xu J , Zeng F , Pang X , Jebessa E , Liang S , Nie Q , Zhang X
Ref : Poult Sci , 95 :99 , 2016
Abstract : Abhydrolase domain containing 5 gene (ABHD5), also known as comparative gene identification 58 (CGI-58), is a member of the alpha/beta-hydrolase family as a protein cofactor of ATGL stimulating its triacylglycerol hydrolase activity. In this study, we aim to characterize the expression and variations of ABHD5 and to study their functions in chicken fat metabolism. We compared the ABHD5 expression level in various tissues and under different nutrition conditions, identified the variations of ABHD5, and associated them with production traits in an F2 resource population of chickens. Overexpression analysis with two different genotypes and siRNA interfering analysis of ABHD5 were performed in chicken preadipocytes. Chicken ABDH5 was expressed widely and most predominantly in adipose tissue. Five SNPs of the ABHD5 gene were identified and genotyped in the F2 resource population. The c.490C > T SNP was associated with subcutaneous fat thickness (P < 0.01), carcass weight (P < 0.05), body weight (P < 0.05), shank diameter (P < 0.05), and shank length (P < 0.05). The c.423T > C SNP was also associated with chicken body weight (P < 0.05) and shank diameter (P < 0.05). In chicken preadipocytes, overexpression of wild type ABDH5 did not affect the mRNA level of ATGL (adipose triglyceride lipase) but markedly decreased (P < 0.05) the TG (triglyceride) content of the cell, whereas overexpression of mutation type ABHD5 did not affect either ATGL expression or TG content of the cell. The expression of ATGL and TG content of the cell were decreased (P < 0.05) after ABHD5 knockdown in preadipocytes. The mRNA level of ABHD5 was regulated by both feeding and fasting, and by consumption of a high fat diet. It was increased greatly by fasting (P < 0.05) and was returned to control levels after re-feeding in the adipose tissues, and down-regulated in abdominal fat (P < 0.05) and the liver (P < 0.01) of chickens with a high fat diet. These results suggest that expression and variations of ABHD5 may affect fat metabolism through regulating the activity of ATGL in chickens.
ESTHER : Ouyang_2016_Poult.Sci_95_99
PubMedSearch : Ouyang_2016_Poult.Sci_95_99
PubMedID: 26574024

Title : cDNA cloning and characterization of adipose triglyceride lipase gene in zebra finch (Taeniopygia guttata) and Java sparrow (Padda oryzivora) - Hu_2011_DNA.Cell.Biol_30_461
Author(s) : Hu Y , He X , Fang M , Nie Q , Zhang X
Ref : DNA & Cell Biology , 30 :461 , 2011
Abstract : Adipose triglyceride lipase (ATGL) is a crucial lipase that catalyzes triglyceride hydrolysis. In this study, reverse transcription (RT)-polymerase chain reaction, rapid amplification of cDNA ends, and mRNA real-time analysis were performed to characterize the ATGL gene in zebra finch and Java sparrow. Two cDNA of the zebra finch ATGL (zfATGL) gene were 1877 and 1626 bp were obtained; both encoded the same 481 amino acid protein. A 251 bp indel in the 3' UTR led to two variant transcripts. The zfATGL gene at chromosome 5 spanned over 28 Kb in length and comprised 9 exons. As in chicken, both the zfATGL gene and its intron 1 were much longer than mammals, which seemed to be one characteristic of ATGL gene in birds. The zfATGL gene was predominantly expressed in abdominal fat, subcutaneous fat, breast muscle, and leg muscle in both male and female. Two cDNA (1938 and 1885 bp) of the Java sparrow ATGL gene were also obtained, both of which encoded the same 481 amino acid protein. A 53 bp indel in 3' UTR led to two variant transcripts. The zfATGL gene was predominantly expressed in breast muscle, subcutaneous fat, abdominal fat, heart, and leg muscle. ATGL homology analysis showed that GASAG and GCGFLG motifs in the patatin region were conserved among 16 species. It was further indicated that the 251- and 53 bp indels at the 3' UTR of ATGL were potential binding sites for miRNA tgu-miR-2987. In conclusion, our study identified the full length cDNA and their variant transcripts of zfATGL and Java sparrow ATGL, and they were predominantly expressed in muscle, fat, and heart tissues.
ESTHER : Hu_2011_DNA.Cell.Biol_30_461
PubMedSearch : Hu_2011_DNA.Cell.Biol_30_461
PubMedID: 21438752

Title : cDNA cloning, characterization, and variation analysis of chicken adipose triglyceride lipase (ATGL) gene - Nie_2009_Mol.Cell.Biochem_320_67
Author(s) : Nie Q , Fang M , Xie L , Shi J , Zhang X
Ref : Molecular & Cellular Biochemistry , 320 :67 , 2009
Abstract : Adipose triglyceride lipase (ATGL) is an important triglyceride-specific lipase that catalyzes the initial step in triglyceride hydrolysis. In this study, cloning, sequencing, and mRNA real-time analyses were employed to characterize the chicken ATGL gene. We obtained a total of 1,528-bp long chicken ATGL cDNA fragment including 51-bp 5'UTR, 1,452-bp open reading frame (ORF), and 25-bp 3'UTR. The predicted chicken ATGL had 483 amino acids and a molecular weight of 53.5 kDa, giving rise to identities of 66.5%, 67.3%, 68.2%, 64.8%, and 66.5% with that of human, mouse, rat, pig, and cattle, respectively. The chicken ATGL gene spanned over 30,197 bp and comprised of nine exons and eight introns, in which the intron 1 (21,146 bp) was far longer than others. It predominantly expressed in subcutaneous fat and abdominal fat and then in kidney and lung. Very low but detectable mRNA level was also observed in other 15 tissues. However, no mRNA was detected in spleen. A total of 15 single nucleotide polymorphisms (SNPs) were identified in its complete cDNA sequences with an average of one SNP in every 102 bp and a summarized nucleotide diversity of 3.02 x 10(-3). Seven of the 15 SNPs were non-synonymous. All SNPs had allelic frequencies over 5% and could be considered as candidate markers for future marker-trait association analysis.
ESTHER : Nie_2009_Mol.Cell.Biochem_320_67
PubMedSearch : Nie_2009_Mol.Cell.Biochem_320_67
PubMedID: 18679582