Lei X

References (10)

Title : Microbial-host-isozyme analyses reveal microbial DPP4 as a potential antidiabetic target - Wang_2023_Science_381_eadd5787
Author(s) : Wang K , Zhang Z , Hang J , Liu J , Guo F , Ding Y , Li M , Nie Q , Lin J , Zhuo Y , Sun L , Luo X , Zhong Q , Ye C , Yun C , Zhang Y , Wang J , Bao R , Pang Y , Wang G , Gonzalez FJ , Lei X , Qiao J , Jiang C
Ref : Science , 381 :eadd5787 , 2023
Abstract : A mechanistic understanding of how microbial proteins affect the host could yield deeper insights into gut microbiota-host cross-talk. We developed an enzyme activity-screening platform to investigate how gut microbiota-derived enzymes might influence host physiology. We discovered that dipeptidyl peptidase 4 (DPP4) is expressed by specific bacterial taxa of the microbiota. Microbial DPP4 was able to decrease the active glucagon like peptide-1 (GLP-1) and disrupt glucose metabolism in mice with a leaky gut. Furthermore, the current drugs targeting human DPP4, including sitagliptin, had little effect on microbial DPP4. Using high-throughput screening, we identified daurisoline-d4 (Dau-d4) as a selective microbial DPP4 inhibitor that improves glucose tolerance in diabetic mice.
ESTHER : Wang_2023_Science_381_eadd5787
PubMedSearch : Wang_2023_Science_381_eadd5787
PubMedID: 37535747
Gene_locus related to this paper: bactn-BT4193

Title : The screening for marine fungal strains with high potential in alkaloids production by in situ colony assay and LC-MS\/MS based secondary metabolic profiling - Lu_2023_Front.Microbiol_14_1144328
Author(s) : Lu T , Liu Y , Zhou L , Liao Q , Nie Y , Wang X , Lei X , Hong P , Feng Y , Hu X , Zhang Y
Ref : Front Microbiol , 14 :1144328 , 2023
Abstract : BACKGROUND: Alkaloids are the second primary class of secondary metabolites (SMs) from marine organisms, most of which have antioxidant, antitumor, antibacterial, anti-inflammatory, and other activities. However, the SMs obtained by traditional isolation strategies have drawbacks such as highly reduplication and weak bioactivity. Therefore, it is significantly important to establish an efficient strategy for screening strains and mining novel compounds. METHODS: In this study, we utilized in situ colony assay combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the strain with high potential in alkaloids production. The strain was identified by genetic marker genes and morphological analysis. The secondary metabolites from the strain were isolated by the combine use of vacuum liquid chromatography (VLC), ODS column chromatography, and Sephadex LH-20. Their structures were elucidated by 1D/2D NMR, HR-ESI-MS, and other spectroscopic technologies. Finally, these compounds bioactivity were assay, including anti-inflammatory and anti-beta aggregation. RESULTS: Eighteen marine fungi were preliminarily screened for alkaloids production by in situ colony assay using Dragendorff reagent as dye, and nine of them turned orange, which indicated abundant alkaloids. By thin-layer chromatography (TLC), LC-MS/MS, and multiple approaches assisted Feature-Based Molecular Networking (FBMN) analysis of fermentation extracts, a strain ACD-5 (Penicillium mallochii with GenBank accession number OM368350) from sea cucumber gut was selected for its diverse alkaloids profiles especially azaphilones. In bioassays, the crude extracts of ACD-5 in Czapek-dox broth and brown rice medium showed moderate antioxidant, acetylcholinesterase inhibitory, anti-neuroinflammatory, and anti-beta aggregation activities. Three chlorinated azaphilone alkaloids, compounds 1-3 (sclerotioramine, isochromophilone VI, and isochromophilone IX, respectively), were isolated from the fermentation products of ACD-5 in brown rice medium guided by bioactivities and mass spectrometry analysis. Compound 1 had shown remarkable anti-neuroinflammatory activity in liposaccharide induced BV-2 cells. CONCLUSION: In summary, in situ colony screening together with LC-MS/MS, multi-approach assisted FBMN can act as an efficient screening method for strains with potential in alkaloids production.
ESTHER : Lu_2023_Front.Microbiol_14_1144328
PubMedSearch : Lu_2023_Front.Microbiol_14_1144328
PubMedID: 37206330

Title : Structurally diverse steroids from an endophyte of Aspergillus tennesseensis 1022LEF attenuates LPS-induced inflammatory response through the cholinergic anti-inflammatory pathway - Su_2022_Chem.Biol.Interact_362_109998
Author(s) : Su JC , Pan Q , Xu X , Wei X , Lei X , Zhang P
Ref : Chemico-Biological Interactions , 362 :109998 , 2022
Abstract : The emerging cholinergic anti-inflammatory pathway plays a key role in regulating inflammation. Steroids are known to possess remarkable anti-inflammatory activity. However, the links between steroids and the cholinergic anti-inflammatory pathway remain unidentified. In this study, eight steroids (1-8) featuring five different structural types were characterized from an endophytic fungus Aspergillus tennesseensis 1022LEF, and were subsequently evaluated for their potential role in regulating the cholinergic anti-inflammatory pathway. As a result, compound 8, with the best potency, showed remarkable anti-inflammatory activity at the nanomolar to low micromolar level. Further pharmacological study indicated that 8 notably increased alpha7nAchR expression and inhibited the activation of its down-stream signaling pathways. Collectively, the present study not only highlighted the potential correlation between steroids and the cholinergic anti-inflammatory pathway, but also identified 8 as a dual-functional modulator via directly inhibition to acetylcholinesterase as well as up-regulation of alpha7nAchR expression.
ESTHER : Su_2022_Chem.Biol.Interact_362_109998
PubMedSearch : Su_2022_Chem.Biol.Interact_362_109998
PubMedID: 35649461

Title : Heterozygous lipoprotein lipase knockout mice exhibit impaired hematopoietic stem\/progenitor cell compartment - Shi_2021_Animal.Model.Exp.Med_4_418
Author(s) : Shi G , Li X , Li K , Huang Y , Lei X , Bai L , Qin C
Ref : Animal Model Exp Med , 4 :418 , 2021
Abstract : BACKGROUND: Hematopoietic stem cells (HSC) maintain the hematopoietic system homeostasis through self-renewal and multilineage differentiation potential. HSC are regulated by the microenvironment, cytokine signaling, and transcription factors. Recent results have shown that lipid pathways play a key role in the regulation of HSC quiescence, proliferation, and division. However, the mechanism by which lipid metabolism regulates HSC proliferation and differentiation remains to be clarified. Lipoprotein lipase (LPL) is an essential enzyme in the anabolism and catabolism of very low-density lipoprotein, chylomicrons, and triglyceride-rich lipoproteins. METHODS: The percentage of hematopoietic stem/progenitor cells and immune cells were determined by fluorescence-activated cell sorting (FACS). The function and the mechanism of HSCs were analyzed by cell colony forming assay and qPCR analysis. The changes in LPL(+/-) HSC microenvironment were detected by transplantation assays using red fluorescent protein (RFP) transgenic mice. RESULTS: To explore the function of LPL in HSC regulation, heterozygous LPL-knockout mice (LPL(+/-)) were established and analyzed by FACS. LPL(+/-) mice displayed decreased hematopoietic stem/progenitor cell compartments. In vitro single-cell clonogenic assays and cell-cycle assays using FACS promoted the cell cycle and increased proliferation ability. qPCR analysis showed the expression of p57(KIP2) and p21(WAF1/CIP1) in LPL(+/-) mice was upregulated. CONCLUSIONS: LPL(+/-) mice exhibited HSC compartment impairment due to promotion of HSC proliferation, without any effects on the bone marrow (BM) microenvironment.
ESTHER : Shi_2021_Animal.Model.Exp.Med_4_418
PubMedSearch : Shi_2021_Animal.Model.Exp.Med_4_418
PubMedID: 34977493

Title : Long non-coding RNA ABHD11-AS1 promotes colorectal cancer development through regulation of miR-133a\/SOX4 axis - Lei_2018_Biosci.Rep_38_
Author(s) : Lei X , Li L , Duan X
Ref : Bioscience Reports , 38 : , 2018
Abstract : Recently, lncRNA has been verified to regulate the development and progression of tumor. LncRNA ABHD11-AS1 has been proven to serve as an oncogene in several cancers. However, the role of ABHD11-AS1 in colorectal cancer remains totally unknown. In the present study, qRT-PCR assay revealed that ABHD11-AS1 expression was markedly higher in colorectal cancer tissues and cell lines. In addition, patients who displayed overexpression of ABHD11-AS1 showed a significantly poorer progression free survival (PFS) and overall survival (OS) by Kaplan-Meier analysis. Loss-of-function experiments suggested that silencing of ABHD11-AS1 expression could significantly reduce the proliferation, colony formation, migration and invasion of colorectal cancer cells, and increase cell apoptosis. Moreover, bioinformatics analysis, biotin pull-down assay, luciferase reporter assay, and RIP assay disclosed that ABHD11-AS1 straightly interacted with miR-133a. We also found that SOX4 was a downstream target of miR-133a and ABHD11-AS1 subsequently exerted its biological effects via modulating the expression of SOX4 in colorectal cancer cells. Collectively, these findings manifested that the ABHD11-AS1/miR-133a/SOX4 axis may be a cogitable and promising therapeutic target for colorectal cancer.
ESTHER : Lei_2018_Biosci.Rep_38_
PubMedSearch : Lei_2018_Biosci.Rep_38_
PubMedID: 30429229
Gene_locus related to this paper: human-ABHD11

Title : Transcriptional responses in the hepatopancreas of Eriocheir sinensis exposed to deltamethrin - Yang_2017_PLoS.One_12_e0184581
Author(s) : Yang Z , Zhang Y , Jiang Y , Zhu F , Zeng L , Wang Y , Lei X , Yao Y , Hou Y , Xu L , Xiong C , Yang X , Hu K
Ref : PLoS ONE , 12 :e0184581 , 2017
Abstract : Deltamethrin is an important pesticide widely used against ectoparasites. Deltamethrin contamination has resulted in a threat to the healthy breeding of the Chinese mitten crab, Eriocheir sinensis. In this study, we investigated transcriptional responses in the hepatopancreas of E. sinensis exposed to deltamethrin. We obtained 99,087,448, 89,086,478, and 100,117,958 raw sequence reads from control 1, control 2, and control 3 groups, and 92,094,972, 92,883,894, and 92,500,828 raw sequence reads from test 1, test 2, and test 3 groups, respectively. After filtering and quality checking of the raw sequence reads, our analysis yielded 79,228,354, 72,336,470, 81,859,826, 77,649,400, 77,194,276, and 75,697,016 clean reads with a mean length of 150 bp from the control and test groups. After deltamethrin treatment, a total of 160 and 167 genes were significantly upregulated and downregulated, respectively. Gene ontology terms "biological process," "cellular component," and "molecular function" were enriched with respect to cell killing, cellular process, other organism part, cell part, binding, and catalytic. Pathway analysis using the Kyoto Encyclopedia of Genes and Genomes showed that the metabolic pathways were significantly enriched. We found that the CYP450 enzyme system, carboxylesterase, glutathione-S-transferase, and material (including carbohydrate, lipid, protein, and other substances) metabolism played important roles in the metabolism of deltamethrin in the hepatopancreas of E. sinensis. This study revealed differentially expressed genes related to insecticide metabolism and detoxification in E. sinensis for the first time and will help in understanding the toxicity and molecular metabolic mechanisms of deltamethrin in E. sinensis.
ESTHER : Yang_2017_PLoS.One_12_e0184581
PubMedSearch : Yang_2017_PLoS.One_12_e0184581
PubMedID: 28910412

Title : Obesity associated Lyplal1 gene is regulated in diet induced obesity but not required for adipocyte differentiation - Lei_2015_Mol.Cell.Endocrinol_411_207
Author(s) : Lei X , Callaway M , Zhou H , Yang Y , Chen W
Ref : Mol Cell Endocrinol , 411 :207 , 2015
Abstract : Obesity and its associated morbidities represent one of the major and most rapidly expanding health epidemics in the world. Recent genome-wide association studies (GWAS) have identified several variants in LYPLAL1 gene that are significantly associated with central obesity preferentially in females. However, the exact function of this gene in adipose tissue development and obesity remains completely uncharacterized. We found murine Lyplal1 gene demonstrated a depot and sex-specific expression profile in white adipose tissues (WAT), and was significantly reduced in the epididymal and retroperitoneal fats in a murine model of high fat diet induced obesity (DIO). Lyplal1 mRNA was mildly up-regulated during adipogenesis and enriched in mature adipocytes through a PPARgamma-independent mechanism. However, overexpression and knockdown of Lyplal1 did not significantly perturb adipocyte differentiation, triacylglycerol accumulation and/or insulin signaling. These data highlight a depot-specific marked reduction of Lyplal1 transcripts in diet induced obesity but a dispensable role of Lyplal1 in adipose tissue development.
ESTHER : Lei_2015_Mol.Cell.Endocrinol_411_207
PubMedSearch : Lei_2015_Mol.Cell.Endocrinol_411_207
PubMedID: 25958046
Gene_locus related to this paper: human-LYPLAL1 , mouse-lypl1

Title : Draft Genome Sequence of Norvancomycin-Producing Strain Amycolatopsis orientalis CPCC200066 - Lei_2015_Genome.Announc_3_
Author(s) : Lei X , Yuan F , Shi Y , Li X , Wang L , Hong B
Ref : Genome Announc , 3 : , 2015
Abstract : Amycolatopsis orientalis CPCC200066 is an actinomycete that can produce the glycopeptide antibiotic norvancomycin, which has significant inhibitory activity against Gram-positive cocci and bacilli. Here, we report the draft genome sequence of A. orientalis CPCC200066 and identified the genes involved in norvancomycin biosynthesis.
ESTHER : Lei_2015_Genome.Announc_3_
PubMedSearch : Lei_2015_Genome.Announc_3_
PubMedID: 25977416
Gene_locus related to this paper: amyor-a0a193bq04 , amyor-a0a193bty5 , amyor-a0a193bvz8 , amyor-a0a193byg1 , amyor-a0a193by54 , amyor-a0a193c2b2 , amyor-a0a193bzy5 , amyor-a0a193cbs3

Title : Phaeodactylibacter xiamenensis gen. nov., sp. nov., a member of the family Saprospiraceae isolated from the marine alga Phaeodactylum tricornutum - Chen_2014_Int.J.Syst.Evol.Microbiol_64_3496
Author(s) : Chen Z , Lei X , Lai Q , Li Y , Zhang B , Zhang J , Zhang H , Yang L , Zheng W , Tian Y , Yu Z , Xu H , Zheng T
Ref : Int J Syst Evol Microbiol , 64 :3496 , 2014
Abstract : A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, reddish-orange and chemoheterotrophic bacteria, designated strain KD52(T), was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD52(T) was a member of the family Saprospiraceae, forming a distinct lineage with 'Portibacter lacus' KCTC 23747. The 16S rRNA gene sequence similarity between strain KD52(T) and the type strains of species of the family Saprospiraceae ranged from 86% to 89%. Growth occurred at 20-37 degrees C (optimum, 28 degrees C), in the presence of 1-9% (w/v) NaCl (optimum, 2.5%) and at pH 5-8.5 (optimum, pH 6.0). The dominant fatty acids (>10%) of strain KD52(T) were iso-C15:0 (33.1%), iso-C15:1 G (14.8%) and summed feature 3 (comprising C16:1omega7c and/or C16:1omega6c, 13.8%). The major polar lipids were diphosphatidylglycerol, three unidentified phospholipids, four unknown lipids and one unidentified aminolipid. The DNA G+C content was 51 mol% and the major respiratory quinone was menaquinone-7 (MK-7). On the basis of phenotypic data and phylogenetic inference, strain KD52(T) represents a novel species of a new genus, for which the name Phaeodactylibacter xiamenensis gen. nov., sp. nov., is proposed. The type strain is KD52(T) ( = MCCC 1F01213(T) = KCTC 32575(T)).
ESTHER : Chen_2014_Int.J.Syst.Evol.Microbiol_64_3496
PubMedSearch : Chen_2014_Int.J.Syst.Evol.Microbiol_64_3496
PubMedID: 25052393
Gene_locus related to this paper: 9bact-a0a098s2s4 , 9bact-a0a098s0v5

Title : Measurement of the phospholipase activity of endothelial lipase in mouse plasma - Basu_2013_J.Lipid.Res_54_282
Author(s) : Basu D , Lei X , Josekutty J , Hussain MM , Jin W
Ref : J Lipid Res , 54 :282 , 2013
Abstract : Endothelial lipase (EL) is a major negative regulator of plasma HDL levels in mice, rabbits, and most probably, humans. Although this regulatory function is critically dependent on EL's hydrolysis of HDL phospholipids, as yet there is no phospholipase assay specific for EL in plasma. We developed such an assay for the mouse enzyme using a commercially available phospholipid-like fluorescent substrate in combination with an EL neutralizing antibody. The specificity of the assay was established using EL knockout mice and its utility demonstrated by detection of an increase in plasma EL phospholipase activity following exposure of wild-type mice to lipopolysaccharide. The assay revealed that murine pre-heparin plasma does not contain measurable EL activity, indicating that the hydrolysis of HDL phospholipids by EL in vivo likely occurs on the cell surface.
ESTHER : Basu_2013_J.Lipid.Res_54_282
PubMedSearch : Basu_2013_J.Lipid.Res_54_282
PubMedID: 23103358