Reinecke F

References (2)

Title : Genome sequence of the bioplastic-producing Knallgas bacterium Ralstonia eutropha H16 - Pohlmann_2006_Nat.Biotechnol_24_1257
Author(s) : Pohlmann A , Fricke WF , Reinecke F , Kusian B , Liesegang H , Cramm R , Eitinger T , Ewering C , Potter M , Schwartz E , Strittmatter A , Voss I , Gottschalk G , Steinbuchel A , Friedrich B , Bowien B
Ref : Nat Biotechnol , 24 :1257 , 2006
Abstract : The H(2)-oxidizing lithoautotrophic bacterium Ralstonia eutropha H16 is a metabolically versatile organism capable of subsisting, in the absence of organic growth substrates, on H(2) and CO(2) as its sole sources of energy and carbon. R. eutropha H16 first attracted biotechnological interest nearly 50 years ago with the realization that the organism's ability to produce and store large amounts of poly[R-(-)-3-hydroxybutyrate] and other polyesters could be harnessed to make biodegradable plastics. Here we report the complete genome sequence of the two chromosomes of R. eutropha H16. Together, chromosome 1 (4,052,032 base pairs (bp)) and chromosome 2 (2,912,490 bp) encode 6,116 putative genes. Analysis of the genome sequence offers the genetic basis for exploiting the biotechnological potential of this organism and provides insights into its remarkable metabolic versatility.
ESTHER : Pohlmann_2006_Nat.Biotechnol_24_1257
PubMedSearch : Pohlmann_2006_Nat.Biotechnol_24_1257
PubMedID: 16964242
Gene_locus related to this paper: alceu-catD1 , alceu-catD2 , alceu-lipas , alceu-q4w8c9 , cupne-q7wt49 , cupnh-q0jy77 , cupnh-q0k0b3 , cupnh-q0k0b5 , cupnh-q0k0g2 , cupnh-q0k0l5 , cupnh-q0k0q7 , cupnh-q0k2d0 , cupnh-q0k4q3 , cupnh-q0k4r4 , cupnh-q0k9j6 , cupnh-q0ka76 , cupnh-q0kab1 , cupnh-q0kat1 , cupnh-q0kcu9 , cupnh-q0kd98 , cupnh-q0kdv2 , cupnh-q0ke65 , cuppj-metx , cuppj-q472r0 , cuptr-b2agb4 , cuptr-b3r9z0 , cuptr-b3r543 , cupnh-acoc , cupnh-q0jyv4 , cupnh-q0jzs8 , cupnh-q0jzu9 , cupnh-q0k1a8 , cupnh-q0k1c5 , cupnh-q0k2b3 , cupnh-q0k3m7 , cupnh-q0k7y4 , cupnh-q0k9g4 , cupnh-q0k9l1 , cupnh-q0k038 , cupnh-q0k189 , cupnh-q0k199 , cupnh-q0k226 , cupnh-q0k320 , cupnh-q0k399 , cupnh-q0kbr4 , cupnh-q0kbs3 , cupnh-q0kcd2 , cupnh-q0kci6 , cupnh-q0kd51 , cupnh-q0kfc2 , ralpi-u3qr80 , cupnn-g0ewh7 , cupnh-hboh , cupnh-q0kdw6

Title : The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha - Potter_2004_Microbiology_150_2301
Author(s) : Potter M , Muller H , Reinecke F , Wieczorek R , Fricke F , Bowien B , Friedrich B , Steinbuchel A
Ref : Microbiology , 150 :2301 , 2004
Abstract : Analysis of the genome sequence of the polyhydroxyalkanoate- (PHA) accumulating bacterium Ralstonia eutropha strain H16 revealed three homologues (PhaP2, PhaP3 and PhaP4) of the phasin protein PhaP1. PhaP1 is known to constitute the major component of the layer at the surface of poly(3-hydroxybutyrate), poly(3HB), granules. PhaP2, PhaP3 and PhaP4 exhibited 42, 49 and 45 % identity or 61, 62 and 63 % similarity to PhaP1, respectively. The calculated molecular masses of PhaP1, PhaP2, PhaP3 and PhaP4 were 20.0, 20.2, 19.6 and 20.2 kDa, respectively. RT-PCR analysis showed that phaP2, phaP3 and phaP4 were transcribed under conditions permissive for accumulation of poly(3HB). 2D PAGE of the poly(3HB) granule proteome and analysis of the detected proteins by MALDI-TOF clearly demonstrated that PhaP1, PhaP3 and PhaP4 are bound to the poly(3HB) granules in the cells. PhaP3 was expressed at a significantly higher level in PhaP1-negative mutants. Occurrence of an unknown protein with an N-terminal amino-acid sequence identical to that of PhaP2 in crude cellular extracts of R. eutropha had previously been shown by others. Although PhaP2 could not be localized in vivo on poly(3HB) granules, in vitro experiments clearly demonstrated binding of PhaP2 to these granules. Further analysis of complete or partial genomes of other poly(3HB)-accumulating bacteria revealed the existence of multiple phasin homologues in Ralstonia solanacearum, Burkholderia fungorum and Azotobacter vinelandii. These new and unexpected findings should affect our current models of PHA-granule structure and may also have a considerable impact on the establishment of heterologous production systems for PHAs.
ESTHER : Potter_2004_Microbiology_150_2301
PubMedSearch : Potter_2004_Microbiology_150_2301
PubMedID: 15256572
Gene_locus related to this paper: alceu-q6ec20