Simier P

References (5)

Title : A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically - de Saint Germain_2021_Plant.Commun_2_100166
Author(s) : de Saint Germain A , Jacobs A , Brun G , Pouvreau JB , Braem L , Cornu D , Clave G , Baudu E , Steinmetz V , Servajean V , Wicke S , Gevaert K , Simier P , Goormachtig S , Delavault P , Boyer FD
Ref : Plant Commun , 2 :100166 , 2021
Abstract : Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe. In order to germinate, it must perceive various structurally divergent host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identify five putative SL receptors in P. ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination. We demonstrate the high plasticity of PrKAI2d3, which allows it to interact with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with bioactivity comparable to that of ITCs. This study demonstrates that P. ramosa has extended its signal perception system during evolution, a fact that should be considered for the development of specific and efficient biocontrol methods.
ESTHER : de Saint Germain_2021_Plant.Commun_2_100166
PubMedSearch : de Saint Germain_2021_Plant.Commun_2_100166
PubMedID: 34746757
Gene_locus related to this paper: phera-PrKAI2c , phera-PrKAI2d1 , phera-PrKAI2d2 , phera-PrKAI2d3 , phera-PrKAI2d4

Title : A Phelipanche ramosa KAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates - de Saint Germain_2020_Biorxiv__
Author(s) : de Saint Germain A , Jacobs A , Brun G , Pouvreau JB , Braem L , Cornu D , Clave G , Baudu E , Steinmetz V , Servajean V , Wicke S , Gevaert K , Simier P , Goormachti S , Delavault P , Boyer FD
Ref : Biorxiv , : , 2020
Abstract : Phelipanche ramosa is an obligate root-parasitic weed threatening major crops in central Europe. For its germination, it has to perceive various structurally diverging host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identified five putative SL receptors in P. ramosa, of which PrKAI2d3 is involved in seed germination stimulation. We established the high plasticity of PrKAI2d3, allowing interaction with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that the PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrated that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with a bioactivity comparable to that of ITCs. This study highlights that P. ramosa has extended its signal perception system during evolution, a fact to be considered in the development of specific and efficient biocontrol methods.
ESTHER : de Saint Germain_2020_Biorxiv__
PubMedSearch : de Saint Germain_2020_Biorxiv__
PubMedID:

Title : Seed response to strigolactone is controlled by abscisic acid-independent DNA methylation in the obligate root parasitic plant, Phelipanche ramosa L. Pomel - Lechat_2015_J.Exp.Bot_66_3129
Author(s) : Lechat MM , Brun G , Montiel G , Veronesi C , Simier P , Thoiron S , Pouvreau JB , Delavault P
Ref : J Exp Bot , 66 :3129 , 2015
Abstract : Seed dormancy release of the obligate root parasitic plant, Phelipanche ramosa, requires a minimum 4-day conditioning period followed by stimulation by host-derived germination stimulants, such as strigolactones. Germination is then mediated by germination stimulant-dependent activation of PrCYP707A1, an abscisic acid catabolic gene. The molecular mechanisms occurring during the conditioning period that silence PrCYP707A1 expression and regulate germination stimulant response are almost unknown. Here, global DNA methylation quantification associated with pharmacological approaches and cytosine methylation analysis of the PrCYP707A1 promoter were used to investigate the modulation and possible role of DNA methylation during the conditioning period and in the PrCYP707A1 response to GR24, a synthetic strigolactone analogue. Active global DNA demethylation occurs during the conditioning period and is required for PrCYP707A1 activation by GR24 and for subsequent seed germination. Treatment with 5-azacytidine, a DNA-hypomethylating molecule, reduces the length of the conditioning period. Conversely, hydroxyurea, a hypermethylating agent, inhibits PrCYP707A1 expression and seed germination. Methylated DNA immunoprecipitation followed by PCR experiments and bisulfite sequencing revealed that DNA demethylation particularly impacts a 78-nucleotide sequence in the PrCYP707A1 promoter. The results here demonstrate that the DNA methylation status during the conditioning period plays a crucial role independently of abscisic acid in the regulation of P. ramosa seed germination by controlling the strigolactone-dependent expression of PrCYP707A1.
ESTHER : Lechat_2015_J.Exp.Bot_66_3129
PubMedSearch : Lechat_2015_J.Exp.Bot_66_3129
PubMedID: 25821070

Title : PrCYP707A1, an ABA catabolic gene, is a key component of Phelipanche ramosa seed germination in response to the strigolactone analogue GR24 - Lechat_2012_J.Exp.Bot_63_5311
Author(s) : Lechat MM , Pouvreau JB , Peron T , Gauthier M , Montiel G , Veronesi C , Todoroki Y , Le Bizec B , Monteau F , Macherel D , Simier P , Thoiron S , Delavault P
Ref : J Exp Bot , 63 :5311 , 2012
Abstract : After a conditioning period, seed dormancy in obligate root parasitic plants is released by a chemical stimulus secreted by the roots of host plants. Using Phelipanche ramosa as the model, experiments conducted in this study showed that seeds require a conditioning period of at least 4 d to be receptive to the synthetic germination stimulant GR24. A cDNA-AFLP procedure on seeds revealed 58 transcript-derived fragments (TDFs) whose expression pattern changed upon GR24 treatment. Among the isolated TDFs, two up-regulated sequences corresponded to an abscisic acid (ABA) catabolic gene, PrCYP707A1, encoding an ABA 8'-hydroxylase. Using the rapid amplification of cDNA ends method, two full-length cDNAs, PrCYP707A1 and PrCYP707A2, were isolated from seeds. Both genes were always expressed at low levels during conditioning during which an initial decline in ABA levels was recorded. GR24 application after conditioning triggered a strong up-regulation of PrCYP707A1 during the first 18 h, followed by an 8-fold decrease in ABA levels detectable 3 d after treatment. In situ hybridization experiments on GR24-treated seeds revealed a specific PrCYP707A1 mRNA accumulation in the cells located between the embryo and the micropyle. Abz-E2B, a specific inhibitor of CYP707A enzymes, significantly impeded seed germination, proving to be a non-competitive antagonist of GR24 with reversible inhibitory activity. These results demonstrate that P. ramosa seed dormancy release relies on ABA catabolism mediated by the GR24-dependent activation of PrCYP707A1. In addition, in situ hybridization corroborates the putative location of cells receptive to the germination stimulants in seeds.
ESTHER : Lechat_2012_J.Exp.Bot_63_5311
PubMedSearch : Lechat_2012_J.Exp.Bot_63_5311
PubMedID: 22859674

Title : Structure-activity relationship studies of strigolactone-related molecules for branching inhibition in garden pea: molecule design for shoot branching - Boyer_2012_Plant.Physiol_159_1524
Author(s) : Boyer FD , de Saint Germain A , Pillot JP , Pouvreau JB , Chen VX , Ramos S , Stevenin A , Simier P , Delavault P , Beau JM , Rameau C
Ref : Plant Physiol , 159 :1524 , 2012
Abstract : Initially known for their role in the rhizosphere in stimulating the seed germination of parasitic weeds such as the Striga and Orobanche species, and later as host recognition signals for arbuscular mycorrhizal fungi, strigolactones (SLs) were recently rediscovered as a new class of plant hormones involved in the control of shoot branching in plants. Herein, we report the synthesis of new SL analogs and, to our knowledge, the first study of SL structure-activity relationships for their hormonal activity in garden pea (Pisum sativum). Comparisons with their action for the germination of broomrape (Phelipanche ramosa) are also presented. The pea rms1 SL-deficient mutant was used in a SL bioassay based on axillary bud length after direct SL application on the bud. This assay was compared with an assay where SLs were fed via the roots using hydroponics and with a molecular assay in which transcript levels of BRANCHED1, the pea homolog of the maize TEOSINTE BRANCHED1 gene were quantified in axillary buds only 6 h after application of SLs. We have demonstrated that the presence of a Michael acceptor and a methylbutenolide or dimethylbutenolide motif in the same molecule is essential. It was established that the more active analog 23 with a dimethylbutenolide as the D-ring could be used to control the plant architecture without strongly favoring the germination of P. ramosa seeds. Bold numerals refer to numbers of compounds.
ESTHER : Boyer_2012_Plant.Physiol_159_1524
PubMedSearch : Boyer_2012_Plant.Physiol_159_1524
PubMedID: 22723084