Title : A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically - de Saint Germain_2021_Plant.Commun_2_100166 |
Author(s) : de Saint Germain A , Jacobs A , Brun G , Pouvreau JB , Braem L , Cornu D , Clave G , Baudu E , Steinmetz V , Servajean V , Wicke S , Gevaert K , Simier P , Goormachtig S , Delavault P , Boyer FD |
Ref : Plant Commun , 2 :100166 , 2021 |
Abstract :
Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe. In order to germinate, it must perceive various structurally divergent host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identify five putative SL receptors in P. ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination. We demonstrate the high plasticity of PrKAI2d3, which allows it to interact with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with bioactivity comparable to that of ITCs. This study demonstrates that P. ramosa has extended its signal perception system during evolution, a fact that should be considered for the development of specific and efficient biocontrol methods. |
PubMedSearch : de Saint Germain_2021_Plant.Commun_2_100166 |
PubMedID: 34746757 |
Gene_locus related to this paper: phera-PrKAI2c , phera-PrKAI2d1 , phera-PrKAI2d2 , phera-PrKAI2d3 , phera-PrKAI2d4 |
Substrate | 3'-Methyl-GR24 desmethyl-GR24 |
Gene_locus | phera-PrKAI2c phera-PrKAI2d1 phera-PrKAI2d2 phera-PrKAI2d3 phera-PrKAI2d4 |
de Saint Germain A, Jacobs A, Brun G, Pouvreau JB, Braem L, Cornu D, Clave G, Baudu E, Steinmetz V, Servajean V, Wicke S, Gevaert K, Simier P, Goormachtig S, Delavault P, Boyer FD (2021)
A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically
Plant Commun
2 :100166
de Saint Germain A, Jacobs A, Brun G, Pouvreau JB, Braem L, Cornu D, Clave G, Baudu E, Steinmetz V, Servajean V, Wicke S, Gevaert K, Simier P, Goormachtig S, Delavault P, Boyer FD (2021)
Plant Commun
2 :100166