Ullah I

References (8)

Title : Phytochemical Profiling, Antioxidant, Antimicrobial and Cholinesterase Inhibitory Effects of Essential Oils Isolated from the Leaves of Artemisia scoparia and Artemisia absinthium - Khan_2022_Pharmaceuticals.(Basel)_15_1221
Author(s) : Khan FA , Khan NM , Ahmad S , Nasruddin , Aziz R , Ullah I , Almehmadi M , Allahyani M , Alsaiari AA , Aljuaid A
Ref : Pharmaceuticals (Basel) , 15 :1221 , 2022
Abstract : The current studies were focused on the phytochemical profiling of two local wild Artemisia species, Artemisia scoparia and Artemisia absinthium leaves' essential oils, extracted via the hydro distillation method along with evaluation of their antioxidant as well as antimicrobial effects. The constituents of EOs were identified using a combined gas chromatography-mass spectrometric (GC-MS) technique. A total of 25 compounds in A. scoparia essential oil (EOAS) were identified, and 14 compounds with percentage abundance of >1% were tabulated, the major being tocopherol derivatives (47.55%). A total of nine compounds in Artemisia absinthium essential oil (EOAA) were enlisted (% age > 1%), the majority being oleic acid derivatives (41.45%). Strong antioxidant effects were pronounced by the EOAS in DPPH (IC(50) = 285 +/- 0.82 microg/mL) and in ABTS (IC(50) = 295 +/- 0.32 microg/mL) free radical scavenging assays. Both the EOs remained potent in inhibiting the growth of bacterial species; Escherichia coli (55-70%) and Shigella flexneri (60-75%) however remained moderately effective against Bacillus subtilis as well as Staphylococcus aureus. Both EOAS and EOAA strongly inhibited the growth of the tested fungal species, especially Aspergillus species (up to 70%). The oils showed anti-cholinesterase potential by inhibiting both Acetylcholinesterase (AChE; IC(50) = 30 +/- 0.04 microg/mL (EOAS), 32 +/- 0.05 microg/mL (EOAA) and Butyrylcholinesterase (BChE; IC(50) = 34 +/- 0.07 microg/mL (EOAS), 36 +/- 0.03 microg/mL (EOAA). In conclusion, the essential oils of A. scoparia and A. absinthium are promising antioxidant, antimicrobial and anticholinergic agents with a different phytochemical composition herein reported for the first time.
ESTHER : Khan_2022_Pharmaceuticals.(Basel)_15_1221
PubMedSearch : Khan_2022_Pharmaceuticals.(Basel)_15_1221
PubMedID: 36297333

Title : Knockdown of GmD53a confers strigolactones mediated rhizobia interaction and promotes nodulation in soybean - Rehman_2022_PeerJ_10_e12815
Author(s) : Rehman N , Khan FU , Imran M , Rajput SA , Li Y , Ullah I , Akhtar RW , Al-Huqail AA , Askary AE , Khalifa AS , Azhar MT
Ref : PeerJ , 10 :e12815 , 2022
Abstract : BACKGROUND: Strigolactones (SLs) play a key role in modulating plant root growth, shoot branching, and plant-symbiont interaction. However, despite their significance, the components of SL biosynthesis and signaling in soybean and their role in soybean-rhizobia interaction is unknown. METHODS: In this study, we identified and functionally characterized the GmD53a from soybean. The GmD53a ORFs were amplified from root cDNA using primers for GmD53a RNA interference. To induce transgenic hairy roots of soybean, electric shock was used to transform pB7WG1WG2 vectors containing GmD53a knockdown and GUS into K599 strains of Agrobacterium rhizogenes. The hairy roots and nodules were collected and examined for root nodules ratio and RNA was extracted after 4 weeks of rhizobia inoculation. RESULTS: A tissue-specific expression assay showed that GmD53a was differentially expressed in plant parts, predominantly in the stem and nodule. Furthermore, its expression was significantly up-regulated during rhizobia infection and varied with nodule formation. The GmD53a-knockdown chimerical plants were produced to further check its role in soybean nodulation in comparison with control GUS. In knockdown lines, the GmD53a (suppressor of strigolactone MAX2) has a higher number of nodules compared to control lines. Furthermore, the expression levels of several nodulation genes essential for initiation and formation of nodules were altered in GmD53a-knockdown lines. CONCLUSION: The results revealed that SL biosynthesis and signaling are not conserved but also have close interaction between SL and legume rhizobia.
ESTHER : Rehman_2022_PeerJ_10_e12815
PubMedSearch : Rehman_2022_PeerJ_10_e12815
PubMedID: 35116200

Title : In Vivo Assessment of the Ameliorative Impact of Some Medicinal Plant Extracts on Lipopolysaccharide-Induced Multiple Sclerosis in Wistar Rats - Rasool_2022_Molecules_27_
Author(s) : Rasool R , Ullah I , Shahid S , Mubeen B , Imam SS , Alshehri S , Ghoneim MM , Alzarea SI , Murtaza BN , Nadeem MS , Kazmi I
Ref : Molecules , 27 : , 2022
Abstract : Multiple sclerosis is a chronic autoimmune disorder that leads to the demyelination of nerve fibers, which is the major cause of non-traumatic disability all around the world. Herbal plants Nepeta hindustana L., Vitex negundo L., and Argemone albiflora L., in addition to anti-inflammatory and anti-oxidative effects, have shown great potential as neuroprotective agents. The study was aimed to develop a neuroprotective model to study the effectiveness of herbal plants (N. hindustana, V. negundo, and A. albiflora) against multiple sclerosis. The in vivo neuroprotective effects of ethanolic extracts isolated from N. hindustana, V. negundo, and A. albiflora were evaluated in lipopolysaccharides (LPS) induced multiple sclerosis Wistar rat model. The rat models were categorized into seven groups including group A as normal, B as LPS induced diseased group, while C, D, E, F, and G were designed as treatment groups. Histopathological evaluation and biochemical markers including stress and inflammatory (MMP-6, MDA, TNF-alpha, AOPPs, AGEs, NO, IL-17 and IL-2), antioxidant (SOD, GSH, CAT, GPx), DNA damage (Isop-2alpha, 8OHdG) as well as molecular biomarkers (RAGE, Caspase-8, p38) along with glutamate, homocysteine, acetylcholinesterase, and myelin binding protein (MBP) were investigated. The obtained data were analyzed using SPSS version 21 and GraphPad Prism 8.0. The different extract treated groups (C, D, E, F, G) displayed a substantial neuroprotective effect regarding remyelination of axonal terminals and oligodendrocytes migration, reduced lymphocytic infiltrations, and reduced necrosis of Purkinje cells. The levels of stress, inflammatory, and DNA damage markers were observed high in the diseased group B, which were reduced after treatments with plant extracts. The antioxidant activity was significantly reduced in diseased induced group B, however, their levels were raised after treatment with plant extract. Group F (a melange of all the extracts) showed the most significant change among all other treatment groups (C, D, E, G). The communal dose of selected plant extracts regulates neurodegeneration at the cellular level resulting in restoration and remyelination of axonal neurons. Moreover, 400 mg/kg dose of three plants in conjugation (Group F) were found to be more effective in restoring the normal activities of all measured parameters than independent doses (Group C, D, E) and is comparable with standard drug nimodipine (Group G) clinically used for the treatment of multiple sclerosis. The present study, for the first time, reported the clinical evidence of N. hindustana, V. negundo, and A. albiflora against multiple sclerosis and concludes that all three plants showed remyelination as well neuroprotective effects which may be used as a potential natural neurotherapeutic agent against multiple sclerosis.
ESTHER : Rasool_2022_Molecules_27_
PubMedSearch : Rasool_2022_Molecules_27_
PubMedID: 35268709

Title : Carveol Attenuates Seizure Severity and Neuroinflammation in Pentylenetetrazole-Kindled Epileptic Rats by Regulating the Nrf2 Signaling Pathway - Alvi_2021_Oxid.Med.Cell.Longev_2021_9966663
Author(s) : Alvi AM , Al Kury LT , Alattar A , Ullah I , Muhammad AJ , Alshaman R , Shah FA , Khan AU , Feng J , Li S
Ref : Oxid Med Cell Longev , 2021 :9966663 , 2021
Abstract : Epilepsy is a neurodegenerative brain disorder characterized by recurrent seizure attacks. Numerous studies have suggested a strong correlation between oxidative stress and neuroinflammation in several neurodegenerative disorders including epilepsy. This study is aimed at investigating the neuroprotective effects of the natural compound carveol against pentylenetetrazole- (PTZ-) induced kindling and seizure model. Two different doses of carveol (10 mg/kg and 20 mg/kg) were administered to male rats to determine the effects and the effective dose of carveol and to further demonstrate the mechanism of action of nuclear factor E2-related factor (Nrf2) in PTZ-induced kindling model. Our results demonstrated reduced levels of innate antioxidants such as superoxide dismutase (SOD), catalase, glutathione-S-transferase (GST), and glutathione (GSH), associated with elevated lipid peroxidation (LPO) and inflammatory cytokines level such as tumor necrosis factor-alpha (TNF-alpha), and mediators like cyclooxygenase (COX-2) and nuclear factor kappa B (NFkappaB). These detrimental effects exacerbated oxidative stress and provoked a marked neuronal alteration in the cortex and hippocampus of PTZ-intoxicated animals that were associated with upregulated Nrf2 gene expression. Furthermore, carveol treatment positively modulated the antioxidant gene Nrf2 and its downstream target HO-1. To further investigate the role of Nrf2, an inhibitor of Nrf2 called all-trans retinoic acid (ATRA) was used, which further exacerbated PTZ toxicity. Moreover, carveol treatment induced cholinergic system activation by mitigating acetylcholinesterase level which is further linked to attenuated neuroinflammatory cascade. The extent of blood-brain barrier disruption was evaluated based on vascular endothelial growth factor (VEGF) expression. Taken together, our findings suggest that carveol acts as an Nrf2 activator and therefore induces downstream antioxidants and mitigates inflammatory insults through multiple pathways. This eventually alleviates PTZ-induced neuroinflammation and neurodegeneration.
ESTHER : Alvi_2021_Oxid.Med.Cell.Longev_2021_9966663
PubMedSearch : Alvi_2021_Oxid.Med.Cell.Longev_2021_9966663
PubMedID: 34422216

Title : Allicin, an Antioxidant and Neuroprotective Agent, Ameliorates Cognitive Impairment - Nadeem_2021_Antioxidants.(Basel)_11_
Author(s) : Nadeem MS , Kazmi I , Ullah I , Muhammad K , Anwar F
Ref : Antioxidants (Basel) , 11 : , 2021
Abstract : Allicin (diallylthiosulfinate) is a defense molecule produced by cellular contents of garlic (Allium sativum L.). On tissue damage, the non-proteinogenic amino acid alliin (S-allylcysteine sulfoxide) is converted to allicin in an enzyme-mediated process catalysed by alliinase. Allicin is hydrophobic in nature, can efficiently cross the cellular membranes and behaves as a reactive sulfur species (RSS) inside the cells. It is physiologically active molecule with the ability to oxidise the thiol groups of glutathione and between cysteine residues in proteins. Allicin has shown anticancer, antimicrobial, antioxidant properties and also serves as an efficient therapeutic agent against cardiovascular diseases. In this context, the present review describes allicin as an antioxidant, and neuroprotective molecule that can ameliorate the cognitive abilities in case of neurodegenerative and neuropsychological disorders. As an antioxidant, allicin fights the reactive oxygen species (ROS) by downregulation of NOX (NADPH oxidizing) enzymes, it can directly interact to reduce the cellular levels of different types of ROS produced by a variety of peroxidases. Most of the neuroprotective actions of allicin are mediated via redox-dependent pathways. Allicin inhibits neuroinflammation by suppressing the ROS production, inhibition of TLR4/MyD88/NF-kappaB, P38 and JNK pathways. As an inhibitor of cholinesterase and (AChE) and butyrylcholinesterase (BuChE) it can be applied to manage the Alzheimer's disease, helps to maintain the balance of neurotransmitters in case of autism spectrum disorder (ASD) and attention deficit hyperactive syndrome (ADHD). In case of acute traumatic spinal cord injury (SCI) allicin protects neuron damage by regulating inflammation, apoptosis and promoting the expression levels of Nrf2 (nuclear factor erythroid 2-related factor 2). Metal induced neurodegeneration can also be attenuated and cognitive abilities of patients suffering from neurological diseases can be ameliorates by allicin administration.
ESTHER : Nadeem_2021_Antioxidants.(Basel)_11_
PubMedSearch : Nadeem_2021_Antioxidants.(Basel)_11_
PubMedID: 35052591

Title : Flavonoids as Prospective Neuroprotectants and Their Therapeutic Propensity in Aging Associated Neurological Disorders - Ayaz_2019_Front.Aging.Neurosci_11_155
Author(s) : Ayaz M , Sadiq A , Junaid M , Ullah F , Ovais M , Ullah I , Ahmed J , Shahid M
Ref : Front Aging Neurosci , 11 :155 , 2019
Abstract : Modern research has revealed that dietary consumption of flavonoids and flavonoids-rich foods significantly improve cognitive capabilities, inhibit or delay the senescence process and related neurodegenerative disorders including Alzheimer's disease (AD). The flavonoids rich foods such as green tea, cocoa, blue berry and other foods improve the various states of cognitive dysfunction, AD and dementia-like pathological alterations in different animal models. The mechanisms of flavonoids have been shown to be mediated through the inhibition of cholinesterases including acetylcholinesterase (AChE), and butyrylcholinesterase (BChE), beta-secretase (BACE1), free radicals and modulation of signaling pathways, that are implicated in cognitive and neuroprotective functions. Flavonoids interact with various signaling protein pathways like ERK and PI3-kinase/Akt and modulate their actions, thereby leading to beneficial neuroprotective effects. Moreover, they enhance vascular blood flow and instigate neurogenesis particularly in the hippocampus. Flavonoids also hamper the progression of pathological symptoms of neurodegenerative diseases by inhibiting neuronal apoptosis induced by neurotoxic substances including free radicals and beta-amyloid proteins (Abeta). All these protective mechanisms contribute to the maintenance of number, quality of neurons and their synaptic connectivity in the brain. Thus flavonoids can thwart the progression of age-related disorders and can be a potential source for the design and development of new drugs effective in cognitive disorders.
ESTHER : Ayaz_2019_Front.Aging.Neurosci_11_155
PubMedSearch : Ayaz_2019_Front.Aging.Neurosci_11_155
PubMedID: 31293414

Title : Comprehensive Screening of Eight Known Causative Genes in Congenital Hypothyroidism With Gland-in-Situ - Nicholas_2016_J.Clin.Endocrinol.Metab_101_4521
Author(s) : Nicholas AK , Serra EG , Cangul H , Alyaarubi S , Ullah I , Schoenmakers E , Deeb A , Habeb AM , Almaghamsi M , Peters C , Nathwani N , Aycan Z , Saglam H , Bober E , Dattani M , Shenoy S , Murray PG , Babiker A , Willemsen R , Thankamony A , Lyons G , Irwin R , Padidela R , Tharian K , Davies JH , Puthi V , Park SM , Massoud AF , Gregory JW , Albanese A , Pease-Gevers E , Martin H , Brugger K , Maher ER , Chatterjee VK , Anderson CA , Schoenmakers N
Ref : J Clinical Endocrinology Metab , 101 :4521 , 2016
Abstract : CONTEXT: Lower TSH screening cutoffs have doubled the ascertainment of congenital hypothyroidism (CH), particularly cases with a eutopically located gland-in-situ (GIS). Although mutations in known dyshormonogenesis genes or TSHR underlie some cases of CH with GIS, systematic screening of these eight genes has not previously been undertaken. OBJECTIVE: Our objective was to evaluate the contribution and molecular spectrum of mutations in eight known causative genes (TG, TPO, DUOX2, DUOXA2, SLC5A5, SLC26A4, IYD, and TSHR) in CH cases with GIS. Patients, Design, and Setting: We screened 49 CH cases with GIS from 34 ethnically diverse families, using next-generation sequencing. Pathogenicity of novel mutations was assessed in silico.
RESULTS: Twenty-nine cases harbored likely disease-causing mutations. Monogenic defects (19 cases) most commonly involved TG (12), TPO (four), DUOX2 (two), and TSHR (one). Ten cases harbored triallelic (digenic) mutations: TG and TPO (one); SLC26A4 and TPO (three), and DUOX2 and TG (six cases). Novel variants overall included 15 TG, six TPO, and three DUOX2 mutations. Genetic basis was not ascertained in 20 patients, including 14 familial cases.
CONCLUSIONS: The etiology of CH with GIS remains elusive, with only 59% attributable to mutations in TSHR or known dyshormonogenesis-associated genes in a cohort enriched for familial cases. Biallelic TG or TPO mutations most commonly underlie severe CH. Triallelic defects are frequent, mandating future segregation studies in larger kindreds to assess their contribution to variable phenotype. A high proportion ( approximately 41%) of unsolved or ambiguous cases suggests novel genetic etiologies that remain to be elucidated.
ESTHER : Nicholas_2016_J.Clin.Endocrinol.Metab_101_4521
PubMedSearch : Nicholas_2016_J.Clin.Endocrinol.Metab_101_4521
PubMedID: 27525530

Title : Draft Genome Sequence of Entomopathogenic Bacterium Photorhabdus temperata Strain M1021, Isolated from Nematodes - Park_2013_Genome.Announc_1_e00747
Author(s) : Park GS , Khan AR , Hong SJ , Jang EK , Ullah I , Jung BK , Choi J , Yoo NK , Park KJ , Shin JH
Ref : Genome Announc , 1 : , 2013
Abstract : Photorhabdus temperata strain M1021 is an entomopathogenic bacterium belonging to the family Enterobacteriaceae and is symbiotically associated with nematodes. The draft genome sequence of P. temperata strain M1021 consists of 5,598,253 bp with a G+C content of 43.7%, and it has 6,120 protein-coding genes.
ESTHER : Park_2013_Genome.Announc_1_e00747
PubMedSearch : Park_2013_Genome.Announc_1_e00747
PubMedID: 24029767
Gene_locus related to this paper: phote-u7r4p1 , phote-t0qme7 , phote-a0a081s150