da Silva FR

References (5)

Title : Investigation of pesticide exposure by genotoxicological, biochemical, genetic polymorphic and in silico analysis - de Oliveira_2019_Ecotoxicol.Environ.Saf_179_135
Author(s) : de Oliveira AFB , de Souza MR , Benedetti D , Scotti AS , Piazza LS , Garcia ALH , Dias JF , Niekraszewicz LAB , Duarte A , Bauer D , Amaral L , Bassi Branco CL , de Melo Reis E , da Silva FR , Da Silva J
Ref : Ecotoxicology & Environmental Safety , 179 :135 , 2019
Abstract : Soybean farmers are exposed to various types of pesticides that contain in their formulations a combination of chemicals with genotoxic and mutagenic potential. Therefore, the objective of this paper was to evaluate the genetic damages caused by this pesticide exposure to soybean producers in the state of Mato Grosso (Brazil), regarding biochemical, genetic polymorphic and in silico analyses. A total of 148 individuals were evaluated, 76 of which were occupationally exposed and 72 were not exposed at all. The buccal micronucleus cytome assay (BMCyt) detected in the exposed group an increase on DNA damage and cell death. No inhibition of butyrylcholinesterase (BchE) was observed within the exposed group. The detection of inorganic elements was made through the particle-induced X-ray emission technique (PIXE), which revealed higher concentrations of Bromine (Br), Rubidium (Rb) and Lead (Pb) in rural workers. A molecular model using in silico analysis suggests how metal ions can cause both DNA damage and apoptosis in the exposed cells. Analysis of the compared effect of X-ray Repair Cross-complement Protein 1 (XRCC1) and Paraoxonase 1 (PON1) genotypes in the groups demonstrated an increase of binucleated cells (exposed group) and nuclear bud (non-exposed group) in individuals with the XRCC1 Trip/- and PON1 Arg/- genes. There was no significant difference in the telomere (TL) mean value in the exposed group in contrast to the non-exposed group. Our results showed that soybean producers showed genotoxic effect and cell death, which may have been induced by exposure to complex mixtures of agrochemicals and fertilizers. In addition, XRCC1 Arg/Arg could, in some respects, provide protection to individuals.
ESTHER : de Oliveira_2019_Ecotoxicol.Environ.Saf_179_135
PubMedSearch : de Oliveira_2019_Ecotoxicol.Environ.Saf_179_135
PubMedID: 31035247

Title : Role of PON1, SOD2, OGG1, XRCC1, and XRCC4 polymorphisms on modulation of DNA damage in workers occupationally exposed to pesticides - Kahl_2018_Ecotoxicol.Environ.Saf_159_164
Author(s) : Kahl VFS , da Silva FR , Alves JDS , da Silva GF , Picinini J , Dhillon VS , Fenech M , de Souza MR , Dias JF , de Souza CT , Salvador M , Branco CDS , Thiesen FV , Simon D , Da Silva J
Ref : Ecotoxicology & Environmental Safety , 159 :164 , 2018
Abstract : Tobacco farming has been proving to induce poor health outcomes in agricultural workers, genomic instability being the triggering one. This study evaluated influence of PON1 (paraoxonase 1), SOD2 (superoxide dismutase), OGG1 (8-oxoguanine glycosylase), XRCC1 (X-ray repair cross-complementing protein 1), and XRCC4 (X-ray repair cross-complementing protein 4) genes polymorphisms on DNA damage in 121 subjects occupationally exposed to pesticides mixtures and nicotine at tobacco fields and 121 non-exposed individuals. Inorganic elements (Cl, P, S and Zn) and cotinine levels were found increased in farmers, confirming exposure. Results show higher frequencies of buccal micronucleus (MN), nuclear buds (NBUD), binucleated cells (BN) and damage index (comet assay), reduced telomere length (TL), and increased parameters of oxidative stress in farmers compared to non-exposed individuals. PON1 Gln/Gln genotype was associated with increased MN frequency. SOD2 Val/Val showed association with increased frequency of MN and NBUD and decreased antioxidant activity. The XRCC1 Arg/Arg showed protective effect for MN, BN and TL, which was also positively influenced by OGG1 -/Cys. MN was decreased in XRCC4 -/Ile farmers. These genotypes also showed a risk for antioxidant activity. Our study proposes that PON1 and SOD2 variants play a role in xenobiotic-metabolizing system in farmers, while base excision repair (BER) pathway could be the repair mechanism involved in genomic instability suffered by tobacco farmers.
ESTHER : Kahl_2018_Ecotoxicol.Environ.Saf_159_164
PubMedSearch : Kahl_2018_Ecotoxicol.Environ.Saf_159_164
PubMedID: 29747151

Title : Genotoxic assessment in tobacco farmers at different crop times - Da Silva_2014_Sci.Total.Environ_490C_334
Author(s) : da Silva FR , Kvitko K , Rohr P , Abreu MB , Thiesen FV , Da Silva J
Ref : Sci Total Environ , 490C :334 , 2014
Abstract : Agricultural workers engaged in tobacco cultivation are constantly exposed to large amounts of pesticides as well as to the nicotine present in raw tobacco leaves. Pesticides have been considered potential chemical mutagens: experimental data revealed that various agrochemicals possess mutagenic properties. Studies have affirmed that nicotine absorbed through the skin results in the characteristic green tobacco sickness (GTS), an occupational illness reported by tobacco workers. This study sought to determine genotoxic effects in farmers occupationally exposed to agrochemicals and nicotine. Peripheral blood samples were collected from 30 agricultural workers, at different crop times (off-season, during pesticides application and leaf harvest), and 30 were non-exposed. We obtained data on DNA damage detected by the Comet assay and Micronucleus test as biomarker of occupational exposure and effect. The serum cholinesterase level, which in general present relation with exposition to organophosphates and carbamates, as well as serum cotinine level, which is a metabolite of nicotine, were also evaluated. The results showed a significant increase in Damage index and frequency in tobacco farmers compared to the non-exposed group, for all different crop times; and a significant increase in micronucleated cells in the off-season group. No correlation was found between age and exposure time in relation to biomarker tests. The DNA damage was greater in males than in females, but with a significant difference only in off-season group. No difference, in cholinesterase activity, was seen among the group of farmers and non-exposed group. Elevated level of cotinine was observed in leaf harvest group. This investigation suggests increased DNA damage in all tobacco crop stages, calling attention to the significant increase during the off-season and tobacco leaf harvest.
ESTHER : Da Silva_2014_Sci.Total.Environ_490C_334
PubMedSearch : Da Silva_2014_Sci.Total.Environ_490C_334
PubMedID: 24867698

Title : Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa - Van Sluys_2003_J.Bacteriol_185_1018
Author(s) : Van Sluys MA , de Oliveira MC , Monteiro-Vitorello CB , Miyaki CY , Furlan LR , Camargo LE , da Silva AC , Moon DH , Takita MA , Lemos EG , Machado MA , Ferro MI , da Silva FR , Goldman MH , Goldman GH , Lemos MV , El-Dorry H , Tsai SM , Carrer H , Carraro DM , de Oliveira RC , Nunes LR , Siqueira WJ , Coutinho LL , Kimura ET , Ferro ES , Harakava R , Kuramae EE , Marino CL , Giglioti E , Abreu IL , Alves LM , do Amaral AM , Baia GS , Blanco SR , Brito MS , Cannavan FS , Celestino AV , da Cunha AF , Fenille RC , Ferro JA , Formighieri EF , Kishi LT , Leoni SG , Oliveira AR , Rosa VE, Jr. , Sassaki FT , Sena JA , de Souza AA , Truffi D , Tsukumo F , Yanai GM , Zaros LG , Civerolo EL , Simpson AJ , Almeida NF, Jr. , Setubal JC , Kitajima JP
Ref : Journal of Bacteriology , 185 :1018 , 2003
Abstract : Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X. fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grape-growing region of California. Comparative analyses with a previously sequenced X. fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X. fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X. fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.
ESTHER : Van Sluys_2003_J.Bacteriol_185_1018
PubMedSearch : Van Sluys_2003_J.Bacteriol_185_1018
PubMedID: 12533478
Gene_locus related to this paper: xylfa-ACVB , xylfa-cxest , xylfa-metx , xylfa-PD1038 , xylfa-PD1211 , xylfa-PD1300 , xylfa-PD1702 , xylfa-PD2024 , xylfa-pip , xylfa-XF0015 , xylfa-XF0357 , xylfa-XF0754 , xylfa-XF0863 , xylfa-XF1029 , xylfa-XF1181 , xylfa-XF1253 , xylfa-XF1282 , xylfa-XF1356 , xylfa-XF1479 , xylfa-XF1965 , xylfa-XF2330 , xylfa-XF2551

Title : The genome sequence of the plant pathogen Xylella fastidiosa. The Xylella fastidiosa Consortium of the Organization for Nucleotide Sequencing and Analysis - Simpson_2000_Nature_406_151
Author(s) : Simpson AJ , Reinach FC , Arruda P , Abreu FA , Acencio M , Alvarenga R , Alves LM , Araya JE , Baia GS , Baptista CS , Barros MH , Bonaccorsi ED , Bordin S , Bove JM , Briones MR , Bueno MR , Camargo AA , Camargo LE , Carraro DM , Carrer H , Colauto NB , Colombo C , Costa FF , Costa MC , Costa-Neto CM , Coutinho LL , Cristofani M , Dias-Neto E , Docena C , El-Dorry H , Facincani AP , Ferreira AJ , Ferreira VC , Ferro JA , Fraga JS , Franca SC , Franco MC , Frohme M , Furlan LR , Garnier M , Goldman GH , Goldman MH , Gomes SL , Gruber A , Ho PL , Hoheisel JD , Junqueira ML , Kemper EL , Kitajima JP , Krieger JE , Kuramae EE , Laigret F , Lambais MR , Leite LC , Lemos EG , Lemos MV , Lopes SA , Lopes CR , Machado JA , Machado MA , Madeira AM , Madeira HM , Marino CL , Marques MV , Martins EA , Martins EM , Matsukuma AY , Menck CF , Miracca EC , Miyaki CY , Monteriro-Vitorello CB , Moon DH , Nagai MA , Nascimento AL , Netto LE , Nhani A, Jr. , Nobrega FG , Nunes LR , Oliveira MA , de Oliveira MC , de Oliveira RC , Palmieri DA , Paris A , Peixoto BR , Pereira GA , Pereira HA, Jr. , Pesquero JB , Quaggio RB , Roberto PG , Rodrigues V , de MRAJ , de Rosa VE, Jr. , de Sa RG , Santelli RV , Sawasaki HE , da Silva AC , da Silva AM , da Silva FR , da Silva WA, Jr. , da Silveira JF , Silvestri ML , Siqueira WJ , de Souza AA , de Souza AP , Terenzi MF , Truffi D , Tsai SM , Tsuhako MH , Vallada H , Van Sluys MA , Verjovski-Almeida S , Vettore AL , Zago MA , Zatz M , Meidanis J , Setubal JC
Ref : Nature , 406 :151 , 2000
Abstract : Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis--a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to 47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.
ESTHER : Simpson_2000_Nature_406_151
PubMedSearch : Simpson_2000_Nature_406_151
PubMedID: 10910347
Gene_locus related to this paper: xylfa-ACVB , xylfa-cxest , xylfa-metx , xylfa-PD2024 , xylfa-pip , xylfa-q9pdj5 , xylfa-XF0015 , xylfa-XF0357 , xylfa-XF0358 , xylfa-XF0754 , xylfa-XF0863 , xylfa-XF0992 , xylfa-XF1029 , xylfa-XF1181 , xylfa-XF1253 , xylfa-XF1282 , xylfa-XF1356 , xylfa-XF1479 , xylfa-XF1743 , xylfa-XF1745 , xylfa-XF1750 , xylfa-XF1829 , xylfa-XF1965 , xylfa-XF2151 , xylfa-XF2260 , xylfa-XF2330 , xylfa-XF2551 , xylfa-XFA0032