Lysin_B_C_ter
Relationship
Comment
Lysin B (LysB), a novel mycolylarabinogalactan esterase from mycobacteriophage, that cleaves the mycolylarabinogalactan bond to release free mycolic acids. The D29 LysB structure shows an alpha/beta hydrolase organization with a catalytic triad common to cutinases, but which contains an additional four-helix domain implicated in the binding of lipid substrates. This superfamily entry represents the linker domain of the mycobacteriophage Lysin B. The linker domain is composed of four alpha-helices connecting the C-terminal alpha-helix back to the side of the central beta-sheet.Family close to cutinases. LysinB_MF is a modular enzyme consisting of an N-terminal peptidoglycan-binding domain (PGBD) linked to the alpha/beta hydrolase domain. Truncation of PGBD significantly enhances the catalytic activity of LysinB_MF.
Database
Interpro : IPR041855 Lysin B, C-terminal linker domain Lysin_B_C_ter
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 3HC7 | Mycobacteriophage Lysin B is a novel mycolylarabinogalactan esterase. | bpmd2-vg12 |
| Gene_locus | Name | Species |
|---|---|---|
| 9caud-Che9dLysB | Mycobacterium phage Che9d Lysin B LysB | Mycobacterium phage Che9d |
| 9caud-PDRPxvLysB | Mycobacterium phage PDRPxv Lysin B | Mycobacterium phage PDRPxv |
| 9caud-RitSunLysB | Mycobacterium phage RitSun (Viruses) Gp37 Lysin B RitSunLysB | Mycobacterium phage RitSun |
| 9caud-YoshiLysB | Mycobacterium phage Yoshi Lysin B | Mycobacterium phage Yoshi |
| 9caud-b5a5z7 | Mycobacterium phages (Porky Gp35\; 244 Gp37\; Cjw1 Gp35\; Kostya Gp36) | Mycobacterium phage Porky |
| 9caud-d3jz76 | Mycobacterium phage RedRock Gp14 | Mycobacterium phage RedRock |
| 9caud-e0ypf9 | Mycobacterium phage LeBron Gp26 | Mycobacterium phage LeBron |
| 9caud-f6m823 | Mycobacterium phage Faith1 Gp28 | Mycobacterium phage Faith1 |
| 9caud-g1d011 | Mycobacterium phage (Mycobacteriophage) Gp53 | Mycobacterium phage Baka |
| 9caud-g1d5a5 | Mycobacterium phage Rey Gp43 | Mycobacterium phage Rey |
| 9caud-g1da59 | Veracruzvirus heldanLysin B | Veracruzvirus heldan |
| 9caud-g1db29 | Mycobacterium phage Timshel Lysin B | Mycobacterium phage Timshel |
| 9caud-g1fga7 | Mycobacterium phage Thibault Gp42 | Mycobacterium phage Thibault |
| 9caud-g1jv64 | Mycobacterium phage Trixie Gp14 | Mycobacterium phage Trixie |
| 9caud-g1jvt5 | Mycobacterium phage Dandelion Gp258 | Mycobacterium phage Dandelion |
| 9caud-g3me08 | Mycobacterium phage Rumpelstiltskin Gp28 | Mycobacterium phage Rumpelstiltskin |
| 9caud-g8i4e1 | Mycobacterium phage DS6A Gp31 | Mycobacterium phage DS6A |
| 9caud-g8i5a5 | Mycobacterium phage Courthouse Gp48 | Mycobacterium phage Courthouse |
| 9caud-g8ib49 | Mycobacterium phage Kugel Gp11 Lysin B | Mycobacterium phage Kugel |
| 9caud-g8ie82 | Mycobacterium phage Bongo Gp38 | Mycobacterium phage Bongo |
| 9caud-h6u5f8 | Mycobacterium phage Fezzik Putative uncharacterized protein | Mycobacterium phage Fezzik |
| 9caud-h9nci8 | Mycobacterium phage Twister Gp10 | Mycobacterium phage Twister |
| 9caud-q19zy6 | Mycobacterium phage Gp31 | Mycobacterium phage Llij |
| 9caud-q856m4 | Mycobacterium phage Corndog Gp70 | Mycobacterium phage Corndog |
| 9caud-w0ljp4 | Mycobacterium phage Echild | Mycobacterium phage Echild |
| 9caud-w6av51 | Mycobacterium phage Saal | Mycobacterium phage Saal |
| 9viru-q9fzr9 | Mycobacterium phage Ms6 Gp3 lysB Ms6LysB | Mycobacterium phage Ms6 |
| bpmb2-q857l1 | Mycobacterium phage Bxz2 (Mycobacteriophage Bxz2\; Wonder) Gp12 | Mycobacterium phage Bxz2 |
| bpmd2-vg12 |  Mycobacterium phage D29 (Mycobacteriophage D29) Gp12 D29LysB |
Mycobacterium phage D29 |
| bpml5-vg12 | Mycobacterium phage L5 (Mycobacteriophage L5) Gp12 | Mycobacterium phage L5 |
| bpmt4-q9zx48 | Mycobacterium phage TM4 (Mycobacteriophage TM4) Lysin B TM4LysB | Mycobacterium phage TM4 |
| mycab-h0i9i3 | Mycobacterium abscessus subsp. bolletii CCUG 48898 = JCM 15300 Uncharacterized protein | Mycobacterium abscessus subsp. bolletii CCUG 48898 = JCM 15300 |
| mycab-i0p9q7 | Mycobacterium abscessus Bacteriophage protein | Mycobacterium abscessus M93 |
| mycfo-WAY18383 | Mycolicibacterium fortuitum (Mycobacterium fortuitum) Peptidoglycan-binding protein | Mycolicibacterium fortuitum |
| mycfo-a0a1a0nu50 | Mycolicibacterium fortuitum (Mycobacterium fortuitum) Peptidoglycan-binding protein LysinB_MF | Mycolicibacterium fortuitum |
| mycxe-i0rp64 | Mycobacterium xenopi, Mycobacterium avium, Gp53 protein | Mycobacterium xenopi RIVM700367 |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Paranitrophenyl-stearate | (4-nitrophenyl) octadecanoate |
References (9)
| Title : The peptidoglycan-binding domain regulates antimycobacterial activity of LysB by affecting its transportation and\/or catalytic activity - Zhang_2026_Commun.Biol__ |
| Author(s) : Zhang L , Zhang Y , Shi Y , Wang F , Chen M , Lv J , Duan Y , Li X , Hu L , He Z , Lu L |
| Ref : Commun Biol , : , 2026 |
| Abstract : |
| PubMedSearch : Zhang_2026_Commun.Biol__ |
| PubMedID: 42260125 |
| Gene_locus related to this paper: bpmt4-q9zx48 , 9viru-q9fzr9 , bpmd2-vg12 |
| Title : The peptidoglycan-binding domain of a mycobacterial prophage-encoded LysinB reveals diverse approaches for domain conservation in hydrolases - Das_2025_Int.J.Biol.Macromol_327_147435 |
| Author(s) : Das R , Nadar K , Arora R , Bajpai U |
| Ref : Int J Biol Macromol , 327 :147435 , 2025 |
| Abstract : |
| PubMedSearch : Das_2025_Int.J.Biol.Macromol_327_147435 |
| PubMedID: 40914364 |
| Gene_locus related to this paper: mycfo-a0a1a0nu50 |
| Title : Unlocking prophage potential: In silico and experimental analysis of a novel Mycobacterium fortuitum LysinB containing a peptidoglycan-binding domain - Das_2024_bioRxiv__ |
| Author(s) : Das R , Nadar K , Arora R , Bajpai U |
| Ref : bioRxiv , : , 2024 |
| Abstract : |
| PubMedSearch : Das_2024_bioRxiv__ |
| PubMedID: 38405724 |
| Gene_locus related to this paper: mycfo-a0a1a0nu50 |
| Title : Discovery and characterization of a novel LysinB from F2 sub-cluster mycobacteriophage RitSun - Arora_2024_Sci.Rep_14_18073 |
| Author(s) : Arora R , Nadar K , Bajpai U |
| Ref : Sci Rep , 14 :18073 , 2024 |
| Abstract : |
| PubMedSearch : Arora_2024_Sci.Rep_14_18073 |
| PubMedID: 39103410 |
| Gene_locus related to this paper: 9caud-RitSunLysB |
| Title : Intrapulmonary Treatment with Mycobacteriophage LysB Rapidly Reduces Mycobacterium abscessus Burden - Hurst-Hess_2023_Antimicrob.Agents.Chemother_67_e0016223 |
| Author(s) : Hurst-Hess K , Walz A , Yang Y , McGuirk H , Gonzalez-Juarrero M , Hatfull GF , Ghosh P , Ojha AK |
| Ref : Antimicrobial Agents & Chemotherapy , 67 :e0016223 , 2023 |
| Abstract : |
| PubMedSearch : Hurst-Hess_2023_Antimicrob.Agents.Chemother_67_e0016223 |
| PubMedID: 37154689 |
| Title : Functional characterization of the endolysins derived from mycobacteriophage PDRPxv - Eniyan_2020_World.J.Microbiol.Biotechnol_36_83 |
| Author(s) : Eniyan K , Sinha A , Ahmad S , Bajpai U |
| Ref : World J Microbiol Biotechnol , 36 :83 , 2020 |
| Abstract : |
| PubMedSearch : Eniyan_2020_World.J.Microbiol.Biotechnol_36_83 |
| PubMedID: 32468233 |
| Gene_locus related to this paper: 9caud-PDRPxvLysB |
| Title : Exploring the Enzymatic and Antibacterial Activities of Novel Mycobacteriophage Lysin B Enzymes - Abouhmad_2020_Int.J.Mol.Sci_21_3176 |
| Author(s) : Abouhmad A , Korany AH , Grey C , Dishisha T , Hatti-Kaul R |
| Ref : Int J Mol Sci , 21 :3176 , 2020 |
| Abstract : |
| PubMedSearch : Abouhmad_2020_Int.J.Mol.Sci_21_3176 |
| PubMedID: 32365915 |
| Gene_locus related to this paper: bpmd2-vg12 , 9caud-w0ljp4 , 9caud-w6av51 , 9caud-g1d011 , 9caud-g8i4e1 |
| Title : Comparative Structural Analysis of Different Mycobacteriophage-Derived Mycolylarabinogalactan Esterases (Lysin B) - Korany_2019_Biomolecules_10_ |
| Author(s) : Korany AH , Abouhmad A , Bakeer W , Essam T , Amin MA , Hatti-Kaul R , Dishisha T |
| Ref : Biomolecules , 10 : , 2019 |
| Abstract : |
| PubMedSearch : Korany_2019_Biomolecules_10_ |
| PubMedID: 31892223 |
| Gene_locus related to this paper: bpmd2-vg12 |
| Title : Mycobacteriophage Lysin B is a novel mycolylarabinogalactan esterase - Payne_2009_Mol.Microbiol_73_367 |
| Author(s) : Payne K , Sun Q , Sacchettini J , Hatfull GF |
| Ref : Molecular Microbiology , 73 :367 , 2009 |
| Abstract : |
| PubMedSearch : Payne_2009_Mol.Microbiol_73_367 |
| PubMedID: 19555454 |
| Gene_locus related to this paper: bpmd2-vg12 |