Lv J

References (14)

Title : Rapid detection of carbamate nerve agent analogues using dually functionalized gold nanoclusters - Zhang_2023_Anal.Bioanal.Chem_415_3275
Author(s) : Zhang Q , Lv J , Xia J , Wang L , Qu G , Yang Y , Liu S
Ref : Anal Bioanal Chem , 415 :3275 , 2023
Abstract : Carbamate nerve agents (CMNAs) are a type of lethal cholinesterase inhibitor with one or more quaternary amine centres and aromatic rings. CMNAs have been recently added to the Annex on Chemicals of the Chemical Weapons Convention (CWC) and Schedules of Controlled Chemicals of China. In this study, a rapid, sensitive and selective method was developed for the fluorescence detection of ambenonium chloride (AC) through host-guest and electrostatic dual interactions between AC and cyclodextrin/11-mercaptoundecanoic acid (CD/MUA) dually functionalized gold nanoclusters (AuNCs). Through this method, AC was detected with a limit of detection of 10.0 ng/mL. Method evaluation showed high selectivity towards AC over other related compounds. The practical applicability was verified, as satisfactory recoveries were obtained for AC spiked in river water and urine, as well as Proficiency Test samples from Organisation for the Prohibition of Chemical Weapons (OPCW). In addition, a fluorescence sensing array comprising four AuNCs was designed to distinguish six carbamates and structurally similar compounds. This method provides a potential approach for the rapid, sensitive and selective recognition and detection of CMNAs.
ESTHER : Zhang_2023_Anal.Bioanal.Chem_415_3275
PubMedSearch : Zhang_2023_Anal.Bioanal.Chem_415_3275
PubMedID: 37266687

Title : Static Binding and Dynamic Transporting-Based Design of Specific Ring-Chain-Ring Acetylcholinesterase Inhibitor: From Galantamine to Natural Product - Zhang_2023_Chemistry__e202203363
Author(s) : Zhang Z , Lv J , Wang Y , Yu H , Guo B , Zhai J , Wang C , Zhao Y , Fan F , Luo W
Ref : Chemistry , :e202203363 , 2023
Abstract : Acetylcholinesterase (AChE) is a key target for the current symptomatic treatment of Alzheimer's disease, and galantamine is a clinical anticholinesterase drug with transiently acting characteristic and good selectivity for AChE. The present theoretical-experimental work improves the drug's residence time without reducing inhibition effect, thus provides crucial breakthrough for modifying the inhibitor of AChE with better-kinetic behavior. The static binding and dynamic delivery properties acquired from atomic view reveal that the galantamine simply occupies catalytic anionic site, and its release from AChE needs only ~ 8.6 kcal/mol. Both of them may cause the short residence time of galantamine. The hotspots and most favorable transport mechanism are identified, and the hydrogen bond and aromatic stacking interactions are observed to play crucial roles for galantamine binding and release in AChE. The typical peripheral anionic site arisen at the delivery process would provide another key occupation to enhance the anti-release ability for inhibitors. The compound with "specific-ring-chain-ring" framework with detail beneficial modify scheme is summarized, which may improve the residence time of inhibitor in AChE. The thermodynamic and dynamic properties of galantamine derivatives are also studied. Based on dictamnine, a natural alkaloid, two novel eligible derivatives are designed, synthesized and evaluated, which verifies our prediction. Multiple computational approaches and experiment combination probably provide a train of thought from static and dynamic view to modify or design appropriate inhibitor on the basis of specific binding and transportation features.
ESTHER : Zhang_2023_Chemistry__e202203363
PubMedSearch : Zhang_2023_Chemistry__e202203363
PubMedID: 36826395

Title : Toxicological effects of trace amounts of pyriproxyfen on the midgut of non-target insect silkworm - Xu_2022_Pestic.Biochem.Physiol_188_105266
Author(s) : Xu K , Lan H , He C , Wei Y , Lu Q , Cai K , Yu D , Yin X , Li Y , Lv J
Ref : Pestic Biochem Physiol , 188 :105266 , 2022
Abstract : Pyriproxyfen is an insect growth regulator that is widely used in public health and pest control in agriculture. Our previous studies have shown that trace amounts of pyriproxyfen in the environment can cause serious toxic effects in the non-target insect silkworm, including failing to pupate, metamorphose and spin cocoons. However, it is unknown why pyriproxyfen not only has no lethal effects on fifth instar larvae but also tend to increase their body weight. The midgut is the main digestive organs of the silkworm, our results showed that the residual of pyriproxyfen in the silkworm at 24sh after 1sxs10(-4)smg/L pyriproxyfen treatment caused severe damage to the midgut microvilli, goblet cells, and nuclei of the silkworm, but body weight and digestibility of the larval were both increased. In addition, pyriproxyfen significantly (ps
ESTHER : Xu_2022_Pestic.Biochem.Physiol_188_105266
PubMedSearch : Xu_2022_Pestic.Biochem.Physiol_188_105266
PubMedID: 36464371

Title : Detoxification mechanism of vinegar-processed Kansui revealed by systematic phytochemical analysis using UPLC-DAD-MS\/MS, UPLC-HR-MS and in silico drug target identification - Liu_2022_Rapid.Commun.Mass.Spectrom__e9332
Author(s) : Liu L , Li J , Lv J , Jiang H , Chen FE
Ref : Rapid Commun Mass Spectrom , :e9332 , 2022
Abstract : RATIONALE: The dried roots of Euphorbia kansui L., known as Kansui, have been used to treat ascites and edema in Traditional Chinese Medicine. However, the toxicity of this herb had seriously restricted its clinical application. A unique vinegar-processing method has been used to reduce its toxicity since ancient China. However, the detoxification mechanism underlying such vinegar-processing has not been fully revealed, to find the answer, the processed-induced components change should be carefully investigated. METHODS: Here we performed systematic analysis of chemical components in raw and vinegar-processed Kansui by UPLC-DAD-MS/MS and UPLC-HR-MS, 31 chemical components in raw and vinegar processed Kansui were found, and the chemical structure of 28 components among them was proposed, and the processed-induced components change was then investigated. RESULTS: A comprehensive conclusion about the processed-induced chemical change was drew. It was found that jatrophane-type diterpenoids decreased markedly after the vinegar-processing, while ingenane-type diterpenoids retained in the vinegar-processing. In silico drug target identification gave hints that jatrophane-type diterpenoids, which decreased markedly during the vinegar-processing, maybe has more intense toxicity involved in cholinesterase and MAPKs, while ingenane-type diterpenoids, which retained in the vinegar-processing, maybe has more intense therapeutic effect involved in carbonic anhydrase. CONCLUSIONS: The possible detoxification mechanism of vinegar-processed Kansui was present. The research has significance on the therapeutic/toxic chemical basis of Kansui, besides, it has significance on drug discovery from terpenoids within the herb.
ESTHER : Liu_2022_Rapid.Commun.Mass.Spectrom__e9332
PubMedSearch : Liu_2022_Rapid.Commun.Mass.Spectrom__e9332
PubMedID: 35716385

Title : Genome-wide identification of the tobacco GDSL family and apical meristem-specific expression conferred by the GDSL promoter - Lv_2021_BMC.Plant.Biol_21_501
Author(s) : Lv J , Dai CB , Wang WF , Sun YH
Ref : BMC Plant Biol , 21 :501 , 2021
Abstract : BACKGROUND: GDSL esterases/lipases are a large protein subfamily defined by the distinct GDSL motif, and play important roles in plant development and stress responses. However, few studies have reported on the role of GDSLs in the growth and development of axillary buds. This work aims to identify the GDSL family members in tobacco and explore whether the NtGDSL gene contributes to development of the axillary bud in tobacco. RESULTS: One hundred fifty-nine GDSL esterase/lipase genes from cultivated tobacco (Nicotiana tabacum) were identified, and the dynamic changes in the expression levels of 93 of these genes in response to topping, as assessed using transcriptome data of topping-induced axillary shoots, were analysed. In total, 13 GDSL esterase/lipase genes responded with changes in expression level. To identify genes and promoters that drive the tissue-specific expression in tobacco apical and axillary buds, the expression patterns of these 13 genes were verified using qRT-PCR. GUS activity and a lethal gene expression pattern driven by the NtGDSL127 promoter in transgenic tobacco demonstrated that NtGDSL127 is specifically expressed in apical buds, axillary buds, and flowers. Three separate deletions in the NtGDSL127 promoter demonstrated that a minimum upstream segment of 235 bp from the translation start site can drive the tissue-specific expression in the apical meristem. Additionally, NtGDSL127 responded to phytohormones, providing strategies for improving tobacco breeding and growth. CONCLUSION: We propose that in tobacco, the NtGDSL127 promoter directs expression specifically in the apical meristem and that expression is closely correlated with axillary bud development.
ESTHER : Lv_2021_BMC.Plant.Biol_21_501
PubMedSearch : Lv_2021_BMC.Plant.Biol_21_501
PubMedID: 34717531

Title : Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis - Wang_2021_Front.Immunol_12_753247
Author(s) : Wang S , Yang H , Guo R , Wang L , Zhang Y , Lv J , Zhao X , Zhang J , Fang H , Zhang Q , Yang J , Cui X , Gao P , Chang T , Gao F
Ref : Front Immunol , 12 :753247 , 2021
Abstract : This study aimed to establish a cell-based assay (CBA) for the detection of agrin antibodies (Agrin-Ab) to explore the clinical features of agrin antibody-positive Chinese patients with myasthenia gravis (Agrin-MG). We developed a CBA based on the human full-length agrin protein expressed in HEK293T cells for the reliable and efficient detection of Agrin-Ab. Clinical data and serum samples were collected from 1948 MG patients in 26 provinces in China. The demographic and clinical features of Agrin-MG patients were compared with those of other MG patient subsets. Eighteen Agrin-MG cases were identified from 1948 MG patients. Nine patients were Agrin-Ab positive, and nine were AChR-Ab and Agrin-Ab double-positive (Agrin/AChR-MG). Eleven (61.11%) patients were males older than 40 years of age. The initial symptom in 13 (81.25%) cases was ocular weakness. Occasionally, the initial symptom was limb-girdle weakness (two cases) or bulbar muscle weakness (one case). Agrin-MG patients demonstrated slight improvement following treatment with either acetylcholinesterase inhibitor or prednisone; however, the combination of the two drugs could effectively relieve MG symptoms. In China, Agrin-MG demonstrated seropositivity rates of 0.92%. These patients were commonly middle-aged or elderly men. The patients usually presented weakness in the ocular, bulbar, and limb muscles, which may be combined with thymoma. These patients have more severe diseases, although the combination of pyridostigmine and prednisone was usually effective in relieving symptoms.
ESTHER : Wang_2021_Front.Immunol_12_753247
PubMedSearch : Wang_2021_Front.Immunol_12_753247
PubMedID: 34956185

Title : Targeted acetylcholinesterase-responsive drug carriers with long duration of drug action and reduced hepatotoxicity - Lin_2019_Int.J.Nanomedicine_14_5817
Author(s) : Lin Y , Wang Y , Lv J , Wang N , Wang J , Li M
Ref : Int J Nanomedicine , 14 :5817 , 2019
Abstract : Purpose: Acetylcholinesterase (AChE) plays a critical role in the transmission of nerve impulse at the cholinergic synapses. Design and synthesis of AChE inhibitors that increase the cholinergic transmission by blocking the degradation of acetylcholine can serve as a strategy for the treatment of AChE-associated disease. Herein, an operational targeted drug delivery platform based on AChE-responsive system has been presented by combining the unique properties of enzyme-controlled mesoporous silica nanoparticles (MSN) with clinical-used AChE inhibitor. Methods: Functionalized MSNs were synthesized by liquid phase method and characterized by using different analytical methods. The biocompatibility and cytotoxicity of MSNs were determined by hemolysis experiment and MTT assay, respectively. Comparison of AChE activity between drug-loading system and inhibitor was developed with kits and by ELISA method. The efficacy of drug-loaded nanocarriers was investigated in a mouse model. Results: Compared with AChE inhibitor itself, the inhibition efficiency of this drug delivery system was strongly dependent on the concentration of AChE. Only AChE with high concentration could cause the opening of pores in the MSN, leading to the controlled release of AChE inhibitor in disease condition. Critically, the drug delivery system can not only exhibit long duration of drug action on AChE inhibition but also reduce the hepatotoxicity in vivo. Conclusion: In summary, AChE-responsive drug release systems have been far less explored. Our results would shed lights on the design of enzyme controlled-release multifunctional system for enzyme-associated disease treatment.
ESTHER : Lin_2019_Int.J.Nanomedicine_14_5817
PubMedSearch : Lin_2019_Int.J.Nanomedicine_14_5817
PubMedID: 31440049

Title : Clinical analysis of Chinese anti-low-density-lipoprotein-receptor-associated protein 4 antibodies in patients with myasthenia gravis - Li_2019_Eur.J.Neurol_26_1296
Author(s) : Li M , Han J , Zhang Y , Lv J , Zhang J , Zhao X , Ren L , Fang H , Yang J , Cui X , Zhang Q , Li Q , Du Y , Gao F
Ref : Eur Journal of Neurology , 26 :1296 , 2019
Abstract : BACKGROUND AND PURPOSE: Low-density-lipoprotein-receptor-associated protein 4 (LRP4) autoantibodies have recently been detected in myasthenia gravis (MG), but little is known about the clinical characteristics associated with this serological type. In this study, the clinical features of Chinese patients with anti-LRP4 antibody-positive MG were characterized. METHODS: A total of 2172 MG serum samples were collected from patients in various parts of China. An enzyme-linked immunosorbent assay was used to detect acetylcholine receptor (AChR) antibody and titin antibody, and cell-based assays were used to detect muscle-specific kinase antibody and LRP4 antibody. Clinical data for patients with MG were collected from different provinces in China. RESULTS: In total, 16 (0.8%) patients with LRP4-MG were found amongst 2172 total patients, including three patients with AChR/LRP4-MG. Additionally, 13 (2.9%) patients with LRP4-MG were found amongst 455 patients with double seronegative MG. The ratio of males to females for these 13 patients was 1:1.6, and 53.8% patients were children. A total of 91.7% of cases exhibited initial ocular involvement, and 58.3% of cases exhibited simple eye muscle involvement. Responses to acetylcholinesterase inhibitors and prednisone were observed. CONCLUSION: The expanded sample confirmed that the positive rate of LRP4 antibodies in China is lower than that in western countries. Our results highlighted the differences between LRP4-MG and other antibody groups. Children and female patients with LRP4-MG have a higher prevalence, often involving the ocular muscles and limb muscles. The clinical symptoms are mild, and satisfactory responses to treatment are often achieved.
ESTHER : Li_2019_Eur.J.Neurol_26_1296
PubMedSearch : Li_2019_Eur.J.Neurol_26_1296
PubMedID: 31050101

Title : Neuroprotective effects of 20(S)-protopanaxatriol (PPT) on scopolamine-induced cognitive deficits in mice - Lu_2018_Phytother.Res_32_1056
Author(s) : Lu C , Lv J , Dong L , Jiang N , Wang Y , Wang Q , Li Y , Chen S , Fan B , Wang F , Liu X
Ref : Phytother Res , 32 :1056 , 2018
Abstract : 20(S)-protopanaxatriol (PPT), one of the ginsenosides from Panax ginseng, has been reported to have neuroprotective effects and to improve memory. The present study was designed to investigate the protective effect of PPT on scopolamine-induced cognitive deficits in mice. Male Institute of Cancer Research mice were pretreated with 2 different doses of PPT (20 and 40 mumol/kg) for 27 days by intraperitoneal injection, and scopolamine (0.75 mg/kg) was injected intraperitoneally for 9 days to induce memory impairment. Thirty minutes after the last pretreatment, the locomotor activity was firstly examined to evaluate the motor function of mice. Then, memory-related behaviors were evaluated, and the related mechanism was further researched. It was founded that PPT treatment significantly reversed scopolamine-induced cognitive impairment in the object location recognition experiment, the Morris water maze test, and the passive avoidance task, showing memory-improving effects. PPT also significantly improved cholinergic system reactivity and suppressed oxidative stress, indicated by inhibition of acetylcholinesterase activity, elevation of acetylcholine levels, increasing superoxide dismutase activity and lowering levels of malondialdehyde in the hippocampus. In addition, the expression levels of Egr-1, c-Jun, and cAMP responsive element binding in the hippocampus were significantly elevated by PPT administration. These results suggest that PPT may be a potential drug candidate for the treatment of cognitive deficit in Alzheimer's disease.
ESTHER : Lu_2018_Phytother.Res_32_1056
PubMedSearch : Lu_2018_Phytother.Res_32_1056
PubMedID: 29468740

Title : Development of tacrine-bifendate conjugates with improved cholinesterase inhibitory and pro-cognitive efficacy and reduced hepatotoxicity - Cen_2017_Eur.J.Med.Chem_144_128
Author(s) : Cen J , Guo H , Hong C , Lv J , Yang Y , Wang T , Fang D , Luo W , Wang C
Ref : Eur Journal of Medicinal Chemistry , 144 :128 , 2017
Abstract : A novel series of tacrine-bifendate (THA-DDB) conjugates (7a-e) were synthesized and evaluated as potential anti-Alzheimer's agents. These compounds showed potent cholinesterase and self-induced beta-amyloid (Abeta) aggregation inhibitory activities. A Lineweaver-Burk plot and molecular modeling study showed that these compounds can target both catalytic active site (CAS) and peripheral anionic site (PAS) of acetylcholinesterase (AChE). The cytotoxicity of the conjugate 7d against PC12 and HepG2 cells and hepatotoxicity against human hepatocyte cell line (HL-7702) were found to be considerably less compared to THA. Moreover, treatment with 7d did not exhibit significant hepatotoxicity in mice. Finally, in vivo studies confirmed that 7d significantly ameliorates the cognitive performances of scopolamine-treated ICR mice. Therefore, 7d has high potential for the treatment of Alzheimer's disease and warrants further investigation.
ESTHER : Cen_2017_Eur.J.Med.Chem_144_128
PubMedSearch : Cen_2017_Eur.J.Med.Chem_144_128
PubMedID: 29268129

Title : Scallop genome provides insights into evolution of bilaterian karyotype and development - Wang_2017_Nat.Ecol.Evol_1_120
Author(s) : Wang S , Zhang J , Jiao W , Li J , Xun X , Sun Y , Guo X , Huan P , Dong B , Zhang L , Hu X , Sun X , Wang J , Zhao C , Wang Y , Wang D , Huang X , Wang R , Lv J , Li Y , Zhang Z , Liu B , Lu W , Hui Y , Liang J , Zhou Z , Hou R , Li X , Liu Y , Li H , Ning X , Lin Y , Zhao L , Xing Q , Dou J , Mao J , Guo H , Dou H , Li T , Mu C , Jiang W , Fu Q , Fu X , Miao Y , Liu J , Yu Q , Li R , Liao H , Kong Y , Jiang Z , Chourrout D , Bao Z
Ref : Nat Ecol Evol , 1 :120 , 2017
Abstract : Reconstructing the genomes of bilaterian ancestors is central to our understanding of animal evolution, where knowledge from ancient and/or slow-evolving bilaterian lineages is critical. Here we report a high-quality, chromosome-anchored reference genome for the scallop Patinopecten yessoensis, a bivalve mollusc that has a slow-evolving genome with many ancestral features. Chromosome-based macrosynteny analysis reveals a striking correspondence between the 19 scallop chromosomes and the 17 presumed ancestral bilaterian linkage groups at a level of conservation previously unseen, suggesting that the scallop may have a karyotype close to that of the bilaterian ancestor. Scallop Hox gene expression follows a new mode of subcluster temporal co-linearity that is possibly ancestral and may provide great potential in supporting diverse bilaterian body plans. Transcriptome analysis of scallop mantle eyes finds unexpected diversity in phototransduction cascades and a potentially ancient Pax2/5/8-dependent pathway for noncephalic eyes. The outstanding preservation of ancestral karyotype and developmental control makes the scallop genome a valuable resource for understanding early bilaterian evolution and biology.
ESTHER : Wang_2017_Nat.Ecol.Evol_1_120
PubMedSearch : Wang_2017_Nat.Ecol.Evol_1_120
PubMedID: 28812685
Gene_locus related to this paper: mizye-a0a210qls6 , mizye-a0a210qis3 , mizye-a0a210qg00 , mizye-a0a210ped6 , mizye-a0a210q4h5 , mizye-a0a210q4h9 , mizye-a0a210q4j1 , mizye-a0a210qf86 , mizye-a0a210q332 , mizye-a0a210pqn0 , mizye-a0a210q7t5 , mizye-a0a210pij5 , mizye-a0a210qyk8 , mizye-a0a210pwl7 , mizye-a0a210q8u5 , mizye-a0a210r5n9 , mizye-a0a210qbv2 , mizye-a0a210pu25 , mizye-a0a210pek1 , mizye-a0a210pul3 , mizye-a0a210pum3 , mizye-a0a210ptr6 , mizye-a0a210ptq5 , mizye-a0a210ptc4.1 , mizye-a0a210ptc4.2 , mizye-a0a210ptv1 , mizye-a0a210ptv7 , mizye-a0a210qgl6 , mizye-a0a210qg90 , mizye-a0a210ptq0 , mizye-a0a210qg72 , mizye-a0a210ptb1 , mizye-a0a210pjd3 , mizye-a0a210qg92 , mizye-a0a210q8v2 , mizye-a0a210qg93 , mizye-a0a210q160.1 , mizye-a0a210q160.2 , mizye-a0a210qes4 , mizye-a0a210pk25 , mizye-a0a210q1b8 , mizye-a0a210q110 , mizye-a0a210r503 , mizye-P021348901.1 , mizye-P021348901.2

Title : 20(S)-protopanaxadiol (PPD) alleviates scopolamine-induced memory impairment via regulation of cholinergic and antioxidant systems, and expression of Egr-1, c-Fos and c-Jun in mice - Lu_2017_Chem.Biol.Interact_279_64
Author(s) : Lu C , Dong L , Lv J , Wang Y , Fan B , Wang F , Liu X
Ref : Chemico-Biological Interactions , 279 :64 , 2017
Abstract : 20(S)-protopanaxadiol (PPD) possesses various biological properties, including anti-inflammatory, antitumor and anti-fatigue properties. Recent studies found that PPD functioned as a neurotrophic agent to ameliorate the sensory deficit caused by glutamate-induced excitotoxicity through its antioxidant effects and exhibited strong antidepressant-like effects in vivo. The objective of the present study was first to investigate the effect of PPD in scopolamine (SCOP)-induced memory deficit in mice and the potential mechanisms involved. In this study, mice were pretreated with PPD (20 and 40 mumol/kg) and donepezil (1.6 mg/kg) intraperitoneally (i.p) for 14 days. Then, open field test was used to assess the effect of PPD on the locomotor activity and mice were daily injected with SCOP (0.75 mg/kg) to induce cognitive deficits and then subjected to behavioral tests by object location recognition (OLR) experiment and Morris water maze (MWM) task. The cholinergic system function, oxidative stress biomarkers and protein expression of Egr-1, c-Fos, and c-Jun in mouse hippocampus were examined. PPD was found to significantly improve the performance of amnesia mice in OLR and MWM tests. PPD regulated cholinergic function by inhibiting SCOP-induced elevation of acetylcholinesterase (AChE) activity, decline of choline acetyltransferase (ChAT) activity and decrease of acetylcholine (Ach) level. PPD suppressed oxidative stress by increasing activities of antioxidant enzymes such as superoxide dismutase (SOD) and lowering maleic diadehyde (MDA) level. Additionally, PPD significantly elevated the expression of Egr-1, c-Fos, and c-Jun in hippocampus at protein level. Taken together, all these results suggested that 20(S)-protopanaxadiol (PPD) may be a candidate compound for the prevention against memory loss in some neurodegenerative diseases such as Alzheimer's disease (AD).
ESTHER : Lu_2017_Chem.Biol.Interact_279_64
PubMedSearch : Lu_2017_Chem.Biol.Interact_279_64
PubMedID: 29133030

Title : Insights into bilaterian evolution from three spiralian genomes - Simakov_2013_Nature_493_526
Author(s) : Simakov O , Marletaz F , Cho SJ , Edsinger-Gonzales E , Havlak P , Hellsten U , Kuo DH , Larsson T , Lv J , Arendt D , Savage R , Osoegawa K , de Jong P , Grimwood J , Chapman JA , Shapiro H , Aerts A , Otillar RP , Terry AY , Boore JL , Grigoriev IV , Lindberg DR , Seaver EC , Weisblat DA , Putnam NH , Rokhsar DS
Ref : Nature , 493 :526 , 2013
Abstract : Current genomic perspectives on animal diversity neglect two prominent phyla, the molluscs and annelids, that together account for nearly one-third of known marine species and are important both ecologically and as experimental systems in classical embryology. Here we describe the draft genomes of the owl limpet (Lottia gigantea), a marine polychaete (Capitella teleta) and a freshwater leech (Helobdella robusta), and compare them with other animal genomes to investigate the origin and diversification of bilaterians from a genomic perspective. We find that the genome organization, gene structure and functional content of these species are more similar to those of some invertebrate deuterostome genomes (for example, amphioxus and sea urchin) than those of other protostomes that have been sequenced to date (flies, nematodes and flatworms). The conservation of these genomic features enables us to expand the inventory of genes present in the last common bilaterian ancestor, establish the tripartite diversification of bilaterians using multiple genomic characteristics and identify ancient conserved long- and short-range genetic linkages across metazoans. Superimposed on this broadly conserved pan-bilaterian background we find examples of lineage-specific genome evolution, including varying rates of rearrangement, intron gain and loss, expansions and contractions of gene families, and the evolution of clade-specific genes that produce the unique content of each genome.
ESTHER : Simakov_2013_Nature_493_526
PubMedSearch : Simakov_2013_Nature_493_526
PubMedID: 23254933
Gene_locus related to this paper: capte-r7t7t5 , capte-r7tx98 , capte-r7ua57 , capte-r7ua73 , capte-ACHE1 , capte-ACHE2 , capte-ACHE3 , capte-ACHE4 , helro-ACHE1 , helro-ACHE1b , lotgi-ACHE1 , lotgi-ACHE2 , lotgi-v4aaa2 , lotgi-v3zx52 , lotgi-v4b4v9 , capte-r7tuq9 , capte-r7v997 , capte-r7vgb9 , lotgi-v3zwe9 , capte-r7tu45 , lotgi-v4bvy3 , lotgi-v3zh31 , capte-r7uie6 , lotgi-v4b898 , capte-r7u3w8 , capte-r7uxb2 , lotgi-v3za62 , capte-r7ux79 , capte-r7uq81 , capte-r7vcc3 , capte-r7ts12 , capte-r7u1x0 , capte-r7uhi1 , capte-r7vei7 , capte-r7v0v3 , lotgi-v4bvi8 , lotgi-v3zyd8 , capte-r7tzy6 , lotgi-v3z9i1 , helro-t1fsg3 , capte-x1yv75 , capte-x2b306 , lotgi-v3zcw8 , capte-r7thp6 , helro-t1fy80 , lotgi-v4bky5 , capte-r7tsq9 , lotgi-v4ali9 , lotgi-v4a9f2 , lotgi-v3zjj3 , helro-t1eej5 , helro-t1g9b7 , capte-r7tiy1 , capte-r7tbl5 , helro-t1exa6 , lotgi-v4a5l7 , helro-t1fm33 , capte-r7ud05 , capte-r7tql8 , capte-r7u5g6 , capte-r7u5z3 , capte-r7ue07 , lotgi-v3zk54 , lotgi-v4a4r1 , lotgi-v4aw76 , lotgi-v4b250 , lotgi-v4bbk1 , lotgi-v3zq85 , lotgi-v4a6s5 , lotgi-v4amq2 , lotgi-v4aqm2 , lotgi-v4crq0 , capte-r7tad7 , capte-r7vgm6 , lotgi-v4agl2 , lotgi-v3zur2 , lotgi-v4aui4 , capte-r7tlv8 , lotgi-v3zu07 , helro-t1g0w9

Title : [Analysis on the association between two polymorphism haplotypes of lipoprotein lipase gene and serum lipids in twins of China] - Huang_2007_Zhonghua.Liu.Xing.Bing.Xue.Za.Zhi_28_523
Author(s) : Huang AQ , Hu YH , Zhan SY , Lv J , Qin Y , Cao WH , Li LM
Ref : Zhonghua Liu Xing Bing Xue Za Zhi , 28 :523 , 2007
Abstract : OBJECTIVE: To investigate the association between haplotypes of S447X and Hind III polymorphisms of lipoprotein lipase (LPL) gene and serum lipids in a population-based twin cohort study in China.
METHODS: Twin subjects were collected based on the twin registry system of China. All twins were investigated by a standard questionnaire and physical examinations. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect the genotypes of S447X and Hind III polymorphisms. Linkage disequilibrium test and haplotypes were estimated between two polymorphisms.
RESULTS: Nine hundred and eighty-seven pairs of twins were eligible for analysis. The two polymorphisms of LPL gene were significantly linkage disequilibrium. In female twins, the H- allele of Hind III polymorphism was significantly related to lower levels of triglycerides(TG) and lower risk of high TG dislipidemia, but those associations disappeared after adjusting the polymorphism of S447X. The H- X haplotype of those two polymorphisms was significantly related to lower TG and TG/HDL (decreasing 12.9% and 14.9% respectively), as well as significantly to lower risk of high TG dislipidemia (OR = 0.40). CONCLUSION: The haplotypes of S447X and Hind III polymorphisms were significantly related to the favorable effect of lipids,but this effect was mostly determined by the polymorphism of S447X, while the effect of Hind III polymorphism was indirectly influenced by the linkage disequilibrium with S447X polymorphism.
ESTHER : Huang_2007_Zhonghua.Liu.Xing.Bing.Xue.Za.Zhi_28_523
PubMedSearch : Huang_2007_Zhonghua.Liu.Xing.Bing.Xue.Za.Zhi_28_523
PubMedID: 17939375