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Family Report for: CFTR-inhibitory-factor_Cif


Block X
Parent Family : Epoxide-hydrolase_like

The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic pathogen that secretes a multitude of virulence factors during the course of infection. Among these is Cif, an epoxide hydrolase (EH) that reduces the functional localization of the cystic fibrosis transmembrane conductance regulator in epithelial cells. In contrast with other EH, it shows a subtitution of an histidine for the second epoxide ring-opening tyrosine in the active site. We used the site around the H177 of the lid defined by Bahl and Madden to build this family: [ED]-X(3)-P-X(13,18)-W-H-[FIA]-X-[FYL]-X(4,7)-[ELT]-X(4)-[GD NAK]-X-[VPE]-X(2)-[FYL]-[LVFI]

IPR016292 (Epoxide hydrolase)
PF12697 (Abhydrolase_6)
no EC number

Peptide in
Nucleotide in
Alignment with Multalin
|Text only/graphic display
Seed alignment with MAFFT
|No colour/coloured with Mview
Alignment with MAFFT
|No colour/coloured with Mview
|Graphical display, obtained with the dnd file produced by Clustalw

3 more
    Title: Pseudomonas aeruginosa Cif defines a distinct class of alpha/beta epoxide hydrolases utilizing a His/Tyr ring-opening pair
    Bahl CD, Madden DR
    Ref: Protein Pept Lett, 19:186, 2012 : PubMed


    Title: Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa
    Bahl CD, Maceachran DP, O'Toole GA, Madden DR
    Ref: Acta Crystallographica Sect F Struct Biol Cryst Commun, 66:26, 2010 : PubMed


    Title: Crystal structure of the CFTR inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor.
    Bahl CD, Morisseau C, Bomberger JM, Stanton BA, Hammock BD, O'Toole GA, Madden DR
    Ref: Journal of Bacteriology, 192:1785, 2010 : PubMed


Other Papers

No structure scheme yet for this family

Structures in CFTR-inhibitory-factor_Cif family (42)

Genes Proteins in CFTR-inhibitory-factor_Cif family (102)

Fragments of genes in CFTR-inhibitory-factor_Cif family (2)

Substrates of some enzymes in the CFTR-inhibitory-factor_Cif family (14)

Inhibitors of some enzymes in the CFTR-inhibitory-factor_Cif family (16)

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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