The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic pathogen that secretes a multitude of virulence factors during the course of infection. Among these is Cif, an epoxide hydrolase (EH) that reduces the functional localization of the cystic fibrosis transmembrane conductance regulator in epithelial cells. In contrast with other EH, it shows a subtitution of an histidine for the second epoxide ring-opening tyrosine in the active site. We used the site around the H177 of the lid defined by Bahl and Madden to build this family: [ED]-X(3)-P-X(13,18)-W-H-[FIA]-X-[FYL]-X(4,7)-[ELT]-X(4)-[GD NAK]-X-[VPE]-X(2)-[FYL]-[LVFI]
Interpro : IPR016292 Epoxide hydrolase
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12697 Abhydrolase_6
Prints : No Print
EC Number : No EC Number
Structures (45)
Genes Proteins in CFTR-inhibitory-factor_Cif family (102)
Fragments of genes in CFTR-inhibitory-factor_Cif family (2)
Structures in CFTR-inhibitory-factor_Cif family (45)Substrates in CFTR-inhibitory-factor_Cif family (14)
Inhibitors in CFTR-inhibitory-factor_Cif family (16)
Title : Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif - Bahl_2016_Biochemistry_55_788 |
Author(s) : Bahl CD , Hvorecny KL , Morisseau C , Gerber SA , Madden DR |
Ref : Biochemistry , 55 :788 , 2016 |
Abstract : Bahl_2016_Biochemistry_55_788 |
ESTHER : Bahl_2016_Biochemistry_55_788 |
PubMedSearch : Bahl_2016_Biochemistry_55_788 |
PubMedID: 26752215 |
Gene_locus related to this paper: pseae-PA2934 |
Title : Rational Design of Potent and Selective Inhibitors of an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa - Kitamura_2016_J.Med.Chem_59_4790 |
Author(s) : Kitamura S , Hvorecny KL , Niu J , Hammock BD , Madden DR , Morisseau C |
Ref : Journal of Medicinal Chemistry , 59 :4790 , 2016 |
Abstract : Kitamura_2016_J.Med.Chem_59_4790 |
ESTHER : Kitamura_2016_J.Med.Chem_59_4790 |
PubMedSearch : Kitamura_2016_J.Med.Chem_59_4790 |
PubMedID: 27120257 |
Gene_locus related to this paper: pseae-PA2934 |
Title : Signature motifs identify an acinetobacter cif virulence factor with epoxide hydrolase activity - Bahl_2014_J.Biol.Chem_289_7460 |
Author(s) : Bahl CD , Hvorecny KL , Bridges AA , Ballok AE , Bomberger JM , Cady KC , O'Toole GA , Madden DR |
Ref : Journal of Biological Chemistry , 289 :7460 , 2014 |
Abstract : Bahl_2014_J.Biol.Chem_289_7460 |
ESTHER : Bahl_2014_J.Biol.Chem_289_7460 |
PubMedSearch : Bahl_2014_J.Biol.Chem_289_7460 |
PubMedID: 24474692 |
Gene_locus related to this paper: pseae-PA2934 , 9gamm-d0bwk6 |
Title : Pseudomonas aeruginosa Cif defines a distinct class of alpha\/beta epoxide hydrolases utilizing a His\/Tyr ring-opening pair - Bahl_2012_Protein.Pept.Lett_19_186 |
Author(s) : Bahl CD , Madden DR |
Ref : Protein Pept Lett , 19 :186 , 2012 |
Abstract : Bahl_2012_Protein.Pept.Lett_19_186 |
ESTHER : Bahl_2012_Protein.Pept.Lett_19_186 |
PubMedSearch : Bahl_2012_Protein.Pept.Lett_19_186 |
PubMedID: 21933119 |
Gene_locus related to this paper: pseae-PA2934 |
Title : Crystal structure of the CFTR inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor. - Bahl_2010_J.Bacteriol_192_1785 |
Author(s) : Bahl CD , Morisseau C , Bomberger JM , Stanton BA , Hammock BD , O'Toole GA , Madden DR |
Ref : Journal of Bacteriology , 192 :1785 , 2010 |
Abstract : Bahl_2010_J.Bacteriol_192_1785 |
ESTHER : Bahl_2010_J.Bacteriol_192_1785 |
PubMedSearch : Bahl_2010_J.Bacteriol_192_1785 |
PubMedID: 20118260 |
Gene_locus related to this paper: pseae-PA2934 |
Title : Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa - Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26 |
Author(s) : Bahl CD , Maceachran DP , O'Toole GA , Madden DR |
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 66 :26 , 2010 |
Abstract : Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26 |
ESTHER : Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26 |
PubMedSearch : Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26 |
PubMedID: 20057063 |
Gene_locus related to this paper: pseae-PA2934 |