Family: CFTR-inhibitory-factor_Cif

Block: X

Parent Family: Epoxide-hydrolase_like


The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic pathogen that secretes a multitude of virulence factors during the course of infection. Among these is Cif, an epoxide hydrolase (EH) that reduces the functional localization of the cystic fibrosis transmembrane conductance regulator in epithelial cells. In contrast with other EH, it shows a subtitution of an histidine for the second epoxide ring-opening tyrosine in the active site. We used the site around the H177 of the lid defined by Bahl and Madden to build this family: [ED]-X(3)-P-X(13,18)-W-H-[FIA]-X-[FYL]-X(4,7)-[ELT]-X(4)-[GD NAK]-X-[VPE]-X(2)-[FYL]-[LVFI]


Interpro : IPR016292 Epoxide hydrolase


Pdoc : No Pdoc

Pfam : PF12697 Abhydrolase_6

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

Structures (45)

Genes Proteins in CFTR-inhibitory-factor_Cif family (102)

Fragments of genes in CFTR-inhibitory-factor_Cif family (2)

Structures in CFTR-inhibitory-factor_Cif family (45)

Substrates in CFTR-inhibitory-factor_Cif family (14)

Inhibitors in CFTR-inhibitory-factor_Cif family (16)

References (6)

Title : Rational Design of Potent and Selective Inhibitors of an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa - Kitamura_2016_J.Med.Chem_59_4790
Author(s) : Kitamura S , Hvorecny KL , Niu J , Hammock BD , Madden DR , Morisseau C
Ref : Journal of Medicinal Chemistry , 59 :4790 , 2016
PubMedID: 27120257
Gene_locus related to this paper: pseae-PA2934

Title : Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif - Bahl_2016_Biochemistry_55_788
Author(s) : Bahl CD , Hvorecny KL , Morisseau C , Gerber SA , Madden DR
Ref : Biochemistry , 55 :788 , 2016
PubMedID: 26752215
Gene_locus related to this paper: pseae-PA2934

Title : Signature motifs identify an acinetobacter cif virulence factor with epoxide hydrolase activity - Bahl_2014_J.Biol.Chem_289_7460
Author(s) : Bahl CD , Hvorecny KL , Bridges AA , Ballok AE , Bomberger JM , Cady KC , O'Toole GA , Madden DR
Ref : Journal of Biological Chemistry , 289 :7460 , 2014
PubMedID: 24474692
Gene_locus related to this paper: pseae-PA2934 , 9gamm-d0bwk6

Title : Pseudomonas aeruginosa Cif defines a distinct class of alpha\/beta epoxide hydrolases utilizing a His\/Tyr ring-opening pair - Bahl_2012_Protein.Pept.Lett_19_186
Author(s) : Bahl CD , Madden DR
Ref : Protein Pept Lett , 19 :186 , 2012
PubMedID: 21933119
Gene_locus related to this paper: pseae-PA2934

Title : Crystal structure of the CFTR inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor. - Bahl_2010_J.Bacteriol_192_1785
Author(s) : Bahl CD , Morisseau C , Bomberger JM , Stanton BA , Hammock BD , O'Toole GA , Madden DR
Ref : Journal of Bacteriology , 192 :1785 , 2010
PubMedID: 20118260
Gene_locus related to this paper: pseae-PA2934

Title : Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa - Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26
Author(s) : Bahl CD , Maceachran DP , O'Toole GA , Madden DR
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 66 :26 , 2010
PubMedID: 20057063
Gene_locus related to this paper: pseae-PA2934