CFTR-inhibitory-factor_Cif
Relationship
Comment
The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic pathogen that secretes a multitude of virulence factors during the course of infection. Among these is Cif, an epoxide hydrolase (EH) that reduces the functional localization of the cystic fibrosis transmembrane conductance regulator in epithelial cells. In contrast with other EH, it shows a subtitution of an histidine for the second epoxide ring-opening tyrosine in the active site. We used the site around the H177 of the lid defined by Bahl and Madden to build this family: [ED]-X(3)-P-X(13,18)-W-H-[FIA]-X-[FYL]-X(4,7)-[ELT]-X(4)-[GD NAK]-X-[VPE]-X(2)-[FYL]-[LVFI]
Database
Interpro : IPR016292 Epoxide hydrolase
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12697 Abhydrolase_6
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 3KD2 | Crystal structure of the CFTR inhibitory factor Cif an epoxide hydrolase virulence factor | pseae-PA2934 |
| 3KDA | Crystal structure of the CFTR inhibitory factor Cif an epoxide hydrolase virulence factor with the H269A mutation | pseae-PA2934 |
| 3PI6 | Crystal structure of the CFTR inhibitory factor Cif with the H177Y mutation | pseae-PA2934 |
| 4DLN | Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation | pseae-PA2934 |
| 4DM7 | Crystal structure of the CFTR inhibitory factor Cif with the E153D mutation | pseae-PA2934 |
| 4DMC | Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation | pseae-PA2934 |
| 4DMF | Crystal structure of the CFTR inhibitory factor Cif with the H177A mutation | pseae-PA2934 |
| 4DMH | Crystal structure of the CFTR inhibitory factor Cif with the H2071 mutation | pseae-PA2934 |
| 4DMK | Crystal structure of the CFTR inhibitory factor Cif with the Y239F mutation | pseae-PA2934 |
| 4DNF | Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the epibromohydrin hydrolysis intermediate | pseae-PA2934 |
| 4DNO | Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the 1,2-epoxyhexane hydrolysis intermediate | pseae-PA2934 |
| 4EHB | Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation bound to epoxyhexane | pseae-PA2934 |
| 4EUS | Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol | pseae-PA2934 |
| 4MEA | Crystal structure of the Cif epoxide hydrolase from Acinetobacter nosocomialis | 9gamm-d0bwk6 |
| 4MEB | Crystal structure of aCif-D158S Cif epoxide hydrolase from Acinetobacter nosocomialis | 9gamm-d0bwk6 |
| 4YX9 | Crystal structure of the CFTR inhibitory factor Cif bound to tiratricol | pseae-PA2934 |
| 5HK9 | Crystal structure of the CFTR inhibitory factor Cif bound to an urea inhibitor | pseae-PA2934 |
| 5HKA | Crystal structure of the CFTR inhibitory factor Cif bound to an amide inhibitor | pseae-PA2934 |
| 5HKB | Crystal structure of the CFTR inhibitory factor Cif bound to the inhibitor KB2115 Eprotirome | pseae-PA2934 |
| 5JYC | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EET hydrolysis intermediate | pseae-PA2934 |
| 5TND | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 7-oxa-bicyclo[4.1.0]heptan (epoxycyclohexane) ECH | pseae-PA2934 |
| 5TNE | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted (2R,3S)-2,3-diphenyloxirane (cis-stilbene oxide) cSO | pseae-PA2934 |
| 5TNF | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 19,20-EDP (epoxy-docosapentaenoic acid) | pseae-PA2934 |
| 5TNG | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EPETE (epoxy-eicosatetraenoic acid) | pseae-PA2934 |
| 5TNH | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 17,18-EpETE (epoxy-eicosatetraenoic acid) hydrolysis intermediate | pseae-PA2934 |
| 5TNI | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted S-Styrene oxide (S-2-phenyloxirane) hydrolysis intermediate S-SOx | pseae-PA2934 |
| 5TNJ | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 4-Vinyl-1-cyclohexene 1,2-epoxide hydrolysis intermediate (VCH) | pseae-PA2934 |
| 5TNK | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted rac-1,2-epoxyoctane (2-hexyloxirane) hydrolysis intermediate (rac-EpO) | pseae-PA2934 |
| 5TNL | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted S-2-butyloxirane (S-1,2-epoxyhexane) hydrolysis intermediate (S-EpH) | pseae-PA2934 |
| 5TNM | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted R-1,2-epoxyoctane (R-2-hexyloxirane) hydrolysis intermediate (R-EpO) | pseae-PA2934 |
| 5TNN | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted S-1,2-epoxyoctane (S-2-hexyloxirane) hydrolysis intermediate (S-EpO) | pseae-PA2934 |
| 5TNP | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted rac-Styrene oxide (rac-2-phenyloxirane) hydrolysis intermediate (rac-SOx) | pseae-PA2934 |
| 5TNQ | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted R-Styrene oxide (R-2-phenyloxirane) hydrolysis intermediate (R-SOx) | pseae-PA2934 |
| 5TNR | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 16,17-EDP (epoxy-docosapentaenoic acid) hydrolysis intermediate | pseae-PA2934 |
| 5TNS | Crystal structure of the D129S mutant of the CFTR inhibitory factor Cif containing the adducted 7-oxa-bicyclo[4.1.0]heptane (epoxycyclohexane) (ECH) hydrolysis intermediate | pseae-PA2934 |
| 6KXH | Streptomyces ambofaciens Alp1U_Y247F mutant in complex with Fluostatin C | stram-q1rqu8 |
| 6KXR | Streptomyces ambofaciens wild type Alp1U from the biosynthesis of kinamycins | stram-q1rqu8 |
| 7AC0 | Epoxide hydrolase CorEH without ligand | corsp-cEH |
| 7CLZ | Streptomyces ambofaciens Alp1U_W187F\/Y247F mutant from the biosynthesis of kinamycins in complex with Fluostatin C | stram-q1rqu8 |
| 7JQX | Crystal structure of Cif-like epoxide hydrolase Cfl1 wild-type from Burkholderia cenocepacia burch-a0b0f8 Bcen_3967 Cfl1 | burch-a0b0f8 |
| 7JQY | Crystal structure of Cif-like epoxide hydrolase Cfl1 D123S mutant from Burkholderia cenocepacia | burch-a0b0f8 |
| 7JQZ | Crystal structure of Cif-like epoxide hydrolase Cfl2 Wild type from Burkholderia cenocepacia | burch-a0az59 |
| 8EVD | Crystal Structure of Nanobody VHH101 Bound to Its Antigen PA14 Cif | pseae-PA2934 |
| 8F6U | Crystal Structure of Nanobody VHH113 Bound to Its Antigen PA14 Cif | pseae-PA2934 |
| 8F6V | Crystal Structure of Nanobody VHH108 Bound to Its Antigen PA14 Cif | pseae-PA2934 |
| Gene_locus | Name | Species |
|---|---|---|
| 9actn-a4ahz3 | marine actinobacterium PHSC20C1 Putative hydrolase | marine actinobacterium PHSC20C1 |
| 9acto-b4ux89 | Streptomyces sp. Mg1 Hydrolase | Streptomyces sp. Mg1 |
| 9acto-b5hut8 | Streptomyces sviceus ATCC 29083 Hydrolase | Streptomyces sviceus ATCC 29083 |
| 9acto-d0wjx8 | Actinomyces sp. oral taxon 848 str. F0332 Epoxide hydrolase | Actinomyces sp. oral taxon 848 str. F0332 |
| 9acto-d1xli1 | Streptomyces sp. ACTE Alpha\/beta hydrolase fold protein | Streptomyces sp. ACTE |
| 9bact-b9xah7.1 | Pedosphaera parvula Ellin514 Alpha\/beta hydrolase fold protein | Pedosphaera parvula Ellin514 |
| 9bact-b9xah7.2 | Pedosphaera parvula Ellin514 Alpha\/beta hydrolase fold protein | Pedosphaera parvula Ellin514 |
| 9entr-b6xkl8 | Providencia alcalifaciens DSM 30120 Putative uncharacterized protein | Providencia alcalifaciens DSM 30120 |
| 9entr-d1p580 | Providencia rustigianii DSM 4541 Alpha\/beta hydrolase | Providencia rustigianii DSM 4541 |
| 9gamm-d0bwk6 |  Acinetobacter sp. RUH2624 Predicted protein |
Acinetobacter sp. RUH2624 |
| 9rhiz-c8sgh4 | Mesorhizobium opportunistum WSM2075 Alpha\/beta hydrolase fold protein | Mesorhizobium opportunistum WSM2075 |
| 9rhiz-c8sn27 | Mesorhizobium opportunistum WSM2075 Alpha\/beta hydrolase fold protein | Mesorhizobium opportunistum WSM2075 |
| 9rhob-b5j801 | Octadecabacter antarcticus 307 Hydrolase, alpha\/beta fold family, putative | Octadecabacter antarcticus 307 |
| 9rhob-b5k859 | Octadecabacter antarcticus 238 Soluble epoxide hydrolase | Octadecabacter antarcticus 238 |
| acica-d0s2j4 | Acinetobacter calcoaceticus RUH2202 Putative uncharacterized protein | Acinetobacter calcoaceticus RUH2202 |
| actmd-c6wg08 | Actinosynnema mirum (strain ATCC 29888 \/ DSM 43827 \/ NBRC 14064 \/ IMR 3971) Alpha\/beta hydrolase fold protein | Actinosynnema mirum |
| actmd-c6whc5 | Actinosynnema mirum (strain ATCC 29888 \/ DSM 43827 \/ NBRC 14064 \/ IMR 3971) Alpha\/beta hydrolase fold protein | Actinosynnema mirum |
| actmd-c6wrs4 | Actinosynnema mirum (strain ATCC 29888 \/ DSM 43827 \/ NBRC 14064 \/ IMR 3971) Alpha\/beta hydrolase fold protein | Actinosynnema mirum |
| agrrk-b9jml0 | Agrobacterium radiobacter (strain K84 \/ ATCC BAA-868) Alpha\/beta hydrolase protein | Agrobacterium radiobacter |
| agrrk-b9jmn1 | Agrobacterium radiobacter (strain K84 \/ ATCC BAA-868) 3-oxoadipate enol-lactone hydrolase\/4-carboxymuconolactone decarboxylase protein | Agrobacterium radiobacter |
| arcb4-a8ety0 | Arcobacter butzleri (strain RM4018) Alpha\/beta hydrolase fold protein | Arcobacter butzleri |
| aspfc-b0y962 | Neosartorya fumigata (strain CEA10 \/ CBS 144.89 \/ FGSC A1163) (Aspergillus fumigatus) Alpha\/beta hydrolase, putative | Neosartorya fumigata |
| aspfn-b8n9z4 | Aspergillus flavus (strain ATCC 200026 \/ FGSC A1120 \/ NRRL 3357 \/ JC 12722 \/ SRRC 167) Epoxide hydrolase, putative | Aspergillus flavus |
| aspnc-a2qh85 | Aspergillus niger (strain CBS 513.88 \/ FGSC A1513) Catalytic activity: cEH of C. sp. acts on epoxides | Aspergillus niger |
| aspor-q2ugd6 | Aspergillus oryzae predicted hydrolases or acyltransferases | Aspergillus oryzae |
| asptn-q0ckr1 | Aspergillus terreus (strain NIH 2624 \/ FGSC A1156) Predicted protein | Aspergillus terreus |
| asptn-q0cp98 | Aspergillus terreus (strain NIH 2624 \/ FGSC A1156) Predicted protein | Aspergillus terreus |
| azoc5-a8ipu7 | Azorhizobium caulinodans (strain ATCC 43989 \/ DSM 5975 \/ ORS 571) Putative hydrolase | Azorhizobium caulinodans |
| beii9-b2ida7 | Beijerinckia indica subsp. indica (strain ATCC 9039 \/ DSM 1715 \/ NCI 8712) Alpha\/beta hydrolase fold | Beijerinckia indica subsp. indica |
| botfb-a6ry56 | Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) Putative uncharacterized protein | Botryotinia fuckeliana |
| braja-BLR8188 | Bradyrhizobium japonicum blr8188 protein | Bradyrhizobium japonicum |
| brasb-a5edz7 | Bradyrhizobium sp. (strain BTAi1 \/ ATCC BAA-1182) Putative hydrolase | Bradyrhizobium sp. |
| brasb-a5emc8 | Bradyrhizobium sp. (strain BTAi1 \/ ATCC BAA-1182) Putative hydrolase | Bradyrhizobium sp. |
| braso-a4ypd9 | Bradyrhizobium sp. (strain ORS278) Putative hydrolase | Bradyrhizobium sp. |
| braso-a4z2a5 | Bradyrhizobium sp. (strain ORS278) Putative hydrolase | Bradyrhizobium sp. |
| burch-a0az59 | Burkholderia cenocepacia Cif-like epoxide hydrolase Bcen_4419 Clf2 |
Burkholderia cenocepacia |
| burch-a0b0f8 | Burkholderia cenocepacia\; Burkholderia cepacia Cif-Like Epoxide Hydrolase Bcen_3967 Cfl1 |
Burkholderia cenocepacia |
| burs3-q390b5 | Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 1776 alpha\/beta hydrolase (EC 3.3.2.3) | Burkholderia sp. |
| burs3-q39nq2 | Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 1776)) alpha\/beta hydrolase (EC 3.3.2.3) | Burkholderia sp. |
| catad-c7q0c0 | Catenulispora acidiphila (strain DSM 44928 \/ NRRL B-24433 \/ NBR 102108 \/ JCM 14897) Alpha\/beta hydrolase fold protein | Catenulispora acidiphila |
| catad-c7qam5 | Catenulispora acidiphila (strain DSM 44928 \/ NRRL B-24433 \/ NBR 102108 \/ JCM 14897) Alpha\/beta hydrolase fold protein | Catenulispora acidiphila |
| causk-b0t349 | Caulobacter sp. (strain K31) Alpha\/beta hydrolase fold | Caulobacter sp. |
| causk-b0t6k7 | Caulobacter sp. (strain K31) Alpha\/beta hydrolase fold | Caulobacter sp. |
| causk-b0t6q0 | Caulobacter sp. (strain K31) Alpha\/beta hydrolase fold | Caulobacter sp. |
| chipd-c7pgn2 | Chitinophaga pinensis (strain ATCC 43595 \/ DSM 2588 \/ NCIB 11800 \/ UQ 2034) Alpha\/beta hydrolase fold protein | Chitinophaga pinensis |
| chipd-c7pgq3 | Chitinophaga pinensis (strain ATCC 43595 \/ DSM 2588 \/ NCIB 11800 \/ UQ 2034) Alpha\/beta hydrolase fold protein | Chitinophaga pinensis |
| chipd-c7plk1 | Chitinophaga pinensis (strain ATCC 43595 \/ DSM 2588 \/ NCIB 11800 \/ UQ 2034) Alpha\/beta hydrolase fold protein | Chitinophaga pinensis |
| chipd-c7plr1 | Chitinophaga pinensis (strain ATCC 43595 \/ DSM 2588 \/ NCIB 11800 \/ UQ 2034) Alpha\/beta hydrolase fold protein | Chitinophaga pinensis |
| corsp-cEH | Corynebacterium sp. cEH gene, CorEH complete CDS |
Corynebacterium sp. |
| delas-a9bwm9 | Delftia acidovorans (strain DSM 14801 \/ SPH-1) Alpha\/beta hydrolase fold | Delftia acidovorans |
| flaj1-a5ff96 | Flavobacterium johnsoniae (strain ATCC 17061 \/ DSM 2064 \/ UW101) (Cytophaga johnsonae) Alpha\/beta hydrolase fold protein | Flavobacterium johnsoniae |
| kinrd-a6w9w5 | Kineococcus radiotolerans (strain ATCC BAA-149 \/ DSM 14245 \/ SRS30216) Alpha\/beta hydrolase fold | Kineococcus radiotolerans |
| korve-q1ivc8 | Candidatus Koribacter versatilis Ellin345 Alpha\/beta hydrolase fold protein | Candidatus Koribacter versatilis Ellin345 |
| krifd-d2pse5 | Kribbella flavida (strain DSM 17836 \/ JCM 10339 \/ NBRC 14399) Alpha\/beta hydrolase fold protein | Kribbella flavida |
| krifd-d2pt36 | Kribbella flavida (strain DSM 17836 \/ JCM 10339 \/ NBRC 14399) Alpha\/beta hydrolase fold protein | Kribbella flavida |
| krifd-d2pts4 | Kribbella flavida (strain DSM 17836 \/ JCM 10339 \/ NBRC 14399) Alpha\/beta hydrolase fold protein | Kribbella flavida |
| lactc-c5dfh7 | Lachancea thermotolerans (strain CBS 6340) (Yeast) (Kluyveromyces thermotolerans) KLTH0D15180p | Lachancea thermotolerans |
| meslo-mlr1864 | Mesorhizobium loti (Rhizobium loti) mlr1864 probable hydrolase | Mesorhizobium loti |
| meslo-mlr2710 | Mesorhizobium loti (Rhizobium loti) mlr2710 | Mesorhizobium loti |
| meslo-mlr4436 | Mesorhizobium loti (Rhizobium loti) mlr4436 contains similarity to hydrolase | Mesorhizobium loti |
| metpb-b1zcn1 | Methylobacterium populi (strain ATCC BAA-705 \/ NCIMB 13946 \/ BJ001) Alpha\/beta hydrolase fold | Methylobacterium populi |
| metrj-b1m809 | Methylobacterium radiotolerans (strain ATCC 27329 \/ DSM 1819 \/ JC 2831) Alpha\/beta hydrolase fold | Methylobacterium radiotolerans |
| mets4-b0u7k5 | Methylobacterium sp. (strain 4-46) Alpha\/beta hydrolase fold | Methylobacterium sp. |
| mobas-q1ydm9 | Aurantimonas manganoxydans SI85-9A1 putative hydrolase, alpha\/beta fold hydrolase superfamily | Aurantimonas manganoxydans SI85-9A1 |
| mycs2-a0r6y9 | Mycobacterium smegmatis (strain ATCC 700084 \/ mc(2)155) Epoxide hydrolase | Mycobacterium smegmatis |
| mycs2-a0r6z0 | Mycobacterium smegmatis (strain ATCC 700084 \/ mc(2)155) Epoxide hydrolase | Mycobacterium smegmatis |
| nithx-q1qpx7 | Nitrobacter hamburgensis (strain X14 \/ DSM 10229) Alpha\/beta hydrolase fold protein | Nitrobacter hamburgensis |
| panam-d4gks2 | Pantoea ananatis (strain LMG 20103) MhpC | Pantoea ananatis |
| pseae-PA2934 | Pseudomonas aeruginosa (strains PA01, PA2934 LESB58\; 2192\; UCBPP-PA14\; C3719) the CFTR inhibitory factor Cif an epoxide hydrolase virulence factor |
Pseudomonas aeruginosa |
| pseae-q1w504 | Pseudomonas aeruginosa alpha\/beta hydrolase family protein | Pseudomonas aeruginosa |
| psef5-q4kax4 | Pseudomonas fluorescens (strain Pf-5 \/ ATCC BAA-477) epoxide hydrolase-related protein | Pseudomonas fluorescens |
| psepf-q3kdj7 | Pseudomonas fluorescens (strain PfO-1) alpha\/beta hydrolase fold precursor | Pseudomonas fluorescens |
| pyrtr-b2vvr4 | Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis) Soluble epoxide hydrolase | Pyrenophora tritici-repentis |
| ralme-q1lmi7 | Ralstonia metallidurans (strain CH34 \/ ATCC 43123 \/ DSM 2839) alpha\/beta hydrolase fold precursor | Ralstonia metallidurans |
| ralp1-c6bhe9 | Ralstonia pickettii (strains 12D\; 12J) Alpha\/beta hydrolase fold protein | Ralstonia pickettii |
| rhisn-c3kly5 | Rhizobium sp. (strain NGR234) Soluble epoxide hydrolase | Rhizobium sp. |
| rhop5-q07i22 | Rhodopseudomonas palustris (strain BisA53) Alpha\/beta hydrolase fold | Rhodopseudomonas palustris |
| sacen-a4fcs3 | Saccharopolyspora erythraea (strain NRRL 23338) Hydrolase | Saccharopolyspora erythraea |
| sacen-a4fjd3 | Saccharopolyspora erythraea (strain NRRL 23338) Alpha\/beta hydrolase | Saccharopolyspora erythraea |
| sacvd-c7mss4 | Saccharomonospora viridis (strain ATCC 15386 \/ DSM 43017 \/ JCM 3036 \/ NBRC 12207 \/ P101) Predicted hydrolase or acyltransferase of alpha\/beta superfamily | Saccharomonospora viridis |
| salto-a4x5w3 | Salinispora tropica (strain ATCC BAA-916 \/ DSM 44818 \/ CNB-440) Alpha\/beta hydrolase fold | Salinispora tropica |
| serod-d1rrw9 | Serratia odorifera 4Rx13 Putative uncharacterized protein | Serratia odorifera 4Rx13 |
| serod-d4dwk1 | Serratia odorifera DSM 4582 Alpha\/beta hydrolase | Serratia odorifera DSM 4582 |
| serp5-a8gev4 | Serratia proteamaculans (strain 568) Alpha\/beta hydrolase fold | Serratia proteamaculans |
| spild-d2qih9 | Spirosoma linguale (strain ATCC 33905 \/ DSM 74 \/ LMG 10896) Alpha\/beta hydrolase fold protein | Spirosoma linguale |
| stanl-d3q5k1 | Stackebrandtia nassauensis (strain DSM 44728 \/ NRRL B-16338 \/ NBR 102104 \/ LLR-40K-21) Alpha\/beta hydrolase fold protein | Stackebrandtia nassauensis |
| stiad-q094u3 | Stigmatella aurantiaca (strain DW4\/3-1) Alpha\/beta hydrolase | Stigmatella aurantiaca |
| stram-q1rqu8 | Streptomyces ambofaciens Alp1U from the biosynthesis of kinamycins |
Streptomyces ambofaciens ATCC 23877 |
| strav-SAV38 | Streptomyces avermitilis putative hydrolase | Streptomyces avermitilis |
| strav-SAV5173 | Streptomyces avermitilis putative hydrolase | Streptomyces avermitilis |
| strcl-b5h2t8 | Streptomyces clavuligerus ATCC 27064 Alpha\/beta hydrolase fold-containing protein | Streptomyces clavuligerus ATCC 27064 |
| strco-2SCG4.13 | Streptomyces coelicolor 2SCG4.13 Scoeh6 | Streptomyces coelicolor |
| strco-SC6D11.36C | Streptomyces coelicolor putative hydrolase | Streptomyces coelicolor |
| strm5-b4sqd6 | Stenotrophomonas maltophilia (strain R551-3) Alpha\/beta hydrolase fold | Stenotrophomonas maltophilia |
| strrd-d2b3g9 | Streptosporangium roseum (strain ATCC 12428 \/ DSM 43021 \/ JCM 3005 \/ NI 9100) Alpha\/beta hydrolase fold protein | Streptosporangium roseum |
| strrd-d2b638 | Streptosporangium roseum (strain ATCC 12428 \/ DSM 43021 \/ JCM 3005 \/ NI 9100) Hydrolase or acyltransferase (Alpha\/beta hydrolase superfamily)-like protein | Streptosporangium roseum |
| synsp-sll1305 | Synechocystis sp. PCC6803 sll1305 | Synechocystis sp. |
| ustma-q4p052 | Ustilago maydis (strain 521 \/ FGSC 9021) (Corn smut fungus) hypothetical protein | Ustilago maydis 521 |
| varps-c5czs2 | Variovorax paradoxus (strain S110) Alpha\/beta hydrolase fold protein | Variovorax paradoxus |
| varps-c5d033 | Variovorax paradoxus (strain S110) Alpha\/beta hydrolase fold protein | Variovorax paradoxus |
| vibal-q1v9y9 | Vibrio alginolyticus 12G01 probable hydrolase | Vibrio alginolyticus 12G01 |
| xanp2-a7ifc0 | Xanthobacter autotrophicus (strain ATCC BAA-1158 \/ Py2) Alpha\/beta hydrolase fold | Xanthobacter autotrophicus |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| phano-q0u517 | Phaeosphaeria nodorum (strain SN15 \/ FGSC 10173) (Glume blotch fungus) (Septoria nodorum) Putative uncharacterized protein | Phaeosphaeria nodorum |
| strfr-q45ra5 | Streptomyces fradiae hypothetical protein | Streptomyces fradiae |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| 1,2-epoxyoctane | 2-hexyloxirane | yarli-q6c598 pseae-PA2934 |
| 14,15-EET | 13-(3-pentyloxiran-2-yl)trideca-5,8,11-trienoic acid | human-EPHX2 human-EPHX3 pseae-PA2934 |
| 14,15-EpETE | (5Z,8Z,11Z)-13-[(2S,3R)-3-[(Z)-pent-2-enyl]oxiran-2-yl]trideca-5,8,11-trienoic acid | pseae-PA2934 |
| 16,17-EDP | 15-[3-(2-Penten-1-yl)-2-oxiranyl]-4,7,10,13-pentadecatetraenoic acid | pseae-PA2934 |
| 17,18-EpETE | (5Z,8Z,11Z,14Z)-16-(3-ethyloxiran-2-yl)hexadeca-5,8,11,14-tetraenoic acid | pseae-PA2934 |
| 19,20-EDP | (4Z,7Z,10Z,13Z,16Z)-18-(3-ethyloxiran-2-yl)octadeca-4,7,10,13,16-pentaenoic acid | pseae-PA2934 mouse-hyes |
| CMNGC | [cyano-(6-methoxynaphthalen-2-yl)methyl] oxiran-2-ylmethyl carbonate | |
| Cis-stilbene-oxide | (2R,3S)-2,3-diphenyloxirane | pseae-PA2934 yeast-SCYNR064C human-EPHX1 ratno-hyep drome-CG15102 arath-At2g26740 mouse-EPHX1 manin-a0a1u9zcc2 |
| Cyclohexene-oxide | (1S,6R)-7-oxabicyclo[4.1.0]heptane | pseae-PA2934 9bact-a0a1u9wz52 9bact-a0a1u9wz58 corsp-cEH 9sphn-a0a191xv39 |
| DPPO | 2-benzyl-3-phenyloxirane | human-EPHX2 mouse-hyes chick-q45qt1 |
| Epibromohydrin | 2-(bromomethyl)oxirane | pseae-PA2934 |
| Fluostatin-C | (3R,4S,6S)-3,10,13-trihydroxy-6-methyl-5-oxapentacyclo[9.7.0.02,8.04,6.012,17]octadeca-1,8,10,12(17),13,15-hexaene-7,18-dione | stram-q1rqu8 |
| S1,2-Epoxy-4-vinylcyclohexane | 4-ethenyl-7-oxabicyclo[4.1.0]heptane | pseae-PA2934 |
| Styrene-oxide | 2-phenyloxirane | pseae-PA2934 9bact-a0a1u9wz52 9bact-a0a1u9wz58 aspng-q1ktb5 9sphn-q3vax0 strsq-b1eph2 phach-b0fsq0 agrme-a0a088b180 rhogl-EPH1 9hyph-a0a126p745 9burk-a0a242m8j4 agrra-echA |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| 1,2-Cyclohexanediol | cyclohexane-1,2-diol | pseae-PA2934 |
| 1,2-Octanediol | (2R)-octane-1,2-diol | pseae-PA2934 |
| 1,2-hexanediol | hexane-1,1-diol | pseae-PA2934 |
| 14,15-EET-product | (5Z,8Z,11Z,14R,15R)-14,15-dihydroxyicosa-5,8,11-trienoic acid | pseae-PA2934 human-EPHX2 |
| 14,15-EpETE-product | (5Z,8E,11Z,14R,15R,17Z)-14,15-dihydroxyicosa-5,8,11,17-tetraenoic acid | pseae-PA2934 |
| 16,17-EDP-product | (4Z,7Z,10Z,13Z,16R,17R,19Z)-16,17-dihydroxydocosa-4,7,10,13,19-pentaenoic acid | pseae-PA2934 |
| 17,18-EpETE-product | 17,18-dihydroxyicosa-5,8,11,14-tetraenoic acid | pseae-PA2934 |
| 19,20-EDP-product | (19S,20R)-19,20-dihydroxydocosa-4,7,10,13,16-pentaenoic acid | pseae-PA2934 |
| 4-ethenylcyclohexane-1,2-diol | 4-ethenylcyclohexane-1,2-diol | pseae-PA2934 |
| Bromopropane-1,2-diol | (2S)-3-bromopropane-1,2-diol | pseae-PA2934 |
| Eprotirome | 3-[3,5-dibromo-4-(4-hydroxy-3-propan-2-ylphenoxy)anilino]-3-oxopropanoic acid | pseae-PA2934 |
| Hydrobenzoin | 1,2-diphenylethane-1,2-diol | pseae-PA2934 |
| Styrene-glycol | (1R)-1-phenylethane-1,2-diol | pseae-PA2934 agrra-echA |
| Tiratricol | 2-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]acetic acid | pseae-PA2934 |
| Tiratricol-derivative-8c | 4-[5-[2,6-dichloro-4-(propanoylamino)phenoxy]-2-hydroxyphenyl]benzamide | pseae-PA2934 |
| Tiratricol-derivative-8h | N-[4-[3-[4-(carbamoylamino)phenyl]-4-hydroxyphenoxy]-3,5-dichlorophenyl]propanamide | pseae-PA2934 |
References (6)
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| Author(s) : Bahl CD , Hvorecny KL , Morisseau C , Gerber SA , Madden DR |
| Ref : Biochemistry , 55 :788 , 2016 |
| Abstract : |
| PubMedSearch : Bahl_2016_Biochemistry_55_788 |
| PubMedID: 26752215 |
| Gene_locus related to this paper: pseae-PA2934 |
| Title : Rational Design of Potent and Selective Inhibitors of an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa - Kitamura_2016_J.Med.Chem_59_4790 |
| Author(s) : Kitamura S , Hvorecny KL , Niu J , Hammock BD , Madden DR , Morisseau C |
| Ref : Journal of Medicinal Chemistry , 59 :4790 , 2016 |
| Abstract : |
| PubMedSearch : Kitamura_2016_J.Med.Chem_59_4790 |
| PubMedID: 27120257 |
| Gene_locus related to this paper: pseae-PA2934 |
| Title : Signature motifs identify an acinetobacter cif virulence factor with epoxide hydrolase activity - Bahl_2014_J.Biol.Chem_289_7460 |
| Author(s) : Bahl CD , Hvorecny KL , Bridges AA , Ballok AE , Bomberger JM , Cady KC , O'Toole GA , Madden DR |
| Ref : Journal of Biological Chemistry , 289 :7460 , 2014 |
| Abstract : |
| PubMedSearch : Bahl_2014_J.Biol.Chem_289_7460 |
| PubMedID: 24474692 |
| Gene_locus related to this paper: pseae-PA2934 , 9gamm-d0bwk6 |
| Title : Pseudomonas aeruginosa Cif defines a distinct class of alpha\/beta epoxide hydrolases utilizing a His\/Tyr ring-opening pair - Bahl_2012_Protein.Pept.Lett_19_186 |
| Author(s) : Bahl CD , Madden DR |
| Ref : Protein Pept Lett , 19 :186 , 2012 |
| Abstract : |
| PubMedSearch : Bahl_2012_Protein.Pept.Lett_19_186 |
| PubMedID: 21933119 |
| Gene_locus related to this paper: pseae-PA2934 |
| Title : Crystal structure of the CFTR inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor. - Bahl_2010_J.Bacteriol_192_1785 |
| Author(s) : Bahl CD , Morisseau C , Bomberger JM , Stanton BA , Hammock BD , O'Toole GA , Madden DR |
| Ref : Journal of Bacteriology , 192 :1785 , 2010 |
| Abstract : |
| PubMedSearch : Bahl_2010_J.Bacteriol_192_1785 |
| PubMedID: 20118260 |
| Gene_locus related to this paper: pseae-PA2934 |
| Title : Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa - Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26 |
| Author(s) : Bahl CD , Maceachran DP , O'Toole GA , Madden DR |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 66 :26 , 2010 |
| Abstract : |
| PubMedSearch : Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26 |
| PubMedID: 20057063 |
| Gene_locus related to this paper: pseae-PA2934 |