pseae-PA2934

Pseudomonas aeruginosa (strains PA01, PA2934 LESB58; 2192; UCBPP-PA14; C3719) the CFTR inhibitory factor Cif an epoxide hydrolase virulence factor

Comment

Relationship

Family : CFTR-inhibitory-factor_Cif

Block : X

Position in NCBI Life Tree : Pseudomonas aeruginosa

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Proteobacteria N E > Gammaproteobacteria N E > Pseudomonadales N E > Pseudomonadaceae N E > Pseudomonas N E > Pseudomonas aeruginosa group N E > Pseudomonas aeruginosa N E

Molecular evidence

No mutation

8EVD - Crystal Structure of Nanobody VHH101 Bound to Its Antigen PA14 Cif 8F6U - Crystal Structure of Nanobody VHH113 Bound to Its Antigen PA14 Cif 8F6V - Crystal Structure of Nanobody VHH108 Bound to Its Antigen PA14 Cif 5JYC - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EET hydrolysis intermediate 5HKA - Crystal structure of the CFTR inhibitory factor Cif bound to an amide inhibitor 5HKB - Crystal structure of the CFTR inhibitory factor Cif bound to the inhibitor KB2115 Eprotirome 5HK9 - Crystal structure of the CFTR inhibitory factor Cif bound to an urea inhibitor 4YX9 - Crystal structure of the CFTR inhibitory factor Cif bound to tiratricol 4DLN - Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation 4DM7 - Crystal structure of the CFTR inhibitory factor Cif with the E153D mutation 5TND - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 7-oxa-bicyclo[4.1.0]heptan (epoxycyclohexane) ECH 5TNE - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted (2R,3S)-2,3-diphenyloxirane (cis-stilbene oxide) cSO 5TNF - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 19,20-EDP (epoxy-docosapentaenoic acid) 5TNG - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EPETE (epoxy-eicosatetraenoic acid) 5TNH - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 17,18-EpETE (epoxy-eicosatetraenoic acid) hydrolysis intermediate 5TNI - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted S-Styrene oxide (S-2-phenyloxirane) hydrolysis intermediate S-SOx 5TNJ - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 4-Vinyl-1-cyclohexene 1,2-epoxide hydrolysis intermediate (VCH) 5TNK - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted rac-1,2-epoxyoctane (2-hexyloxirane) hydrolysis intermediate (rac-EpO) 5TNL - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted S-2-butyloxirane (S-1,2-epoxyhexane) hydrolysis intermediate (S-EpH) 5TNM - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted R-1,2-epoxyoctane (R-2-hexyloxirane) hydrolysis intermediate (R-EpO) 5TNN - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted S-1,2-epoxyoctane (S-2-hexyloxirane) hydrolysis intermediate (S-EpO) 5TNP - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted rac-Styrene oxide (rac-2-phenyloxirane) hydrolysis intermediate (rac-SOx) 5TNQ - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted R-Styrene oxide (R-2-phenyloxirane) hydrolysis intermediate (R-SOx) 5TNR - Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 16,17-EDP (epoxy-docosapentaenoic acid) hydrolysis intermediate 5TNS - Crystal structure of the D129S mutant of the CFTR inhibitory factor Cif containing the adducted 7-oxa-bicyclo[4.1.0]heptane (epoxycyclohexane) (ECH) hydrolysis intermediate 4DMC - Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation 4DMF - Crystal structure of the CFTR inhibitory factor Cif with the H177A mutation 4DMH - Crystal structure of the CFTR inhibitory factor Cif with the H2071 mutation 4DMK - Crystal structure of the CFTR inhibitory factor Cif with the Y239F mutation 4DNF - Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the epibromohydrin hydrolysis intermediate 4DNO - Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the 1,2-epoxyhexane hydrolysis intermediate 4EHB - Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation bound to epoxyhexane 4EUS - Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol 3PI6 - Crystal structure of the CFTR inhibitory factor Cif with the H177Y mutation 3KD2 - Crystal structure of the CFTR inhibitory factor Cif an epoxide hydrolase virulence factor 3KDA - Crystal structure of the CFTR inhibitory factor Cif an epoxide hydrolase virulence factor with the H269A mutation

No kinetic

No disease

Database

Sequence

Peptide

MILDRLCRGL LAGIALTFSL GGFAAEEFPV PNGFESAYRE VDGVKLHYVK GGQGPLVMLV HGFGQTWYEW HQLMPELAKR FTVIAPDLPG LGQSEPPKTG YSGEQVAVYL HKLARQFSPD RPFDLVAHDI GIWNTYPMVV KNQADIARLV YMEAPIPDAR IYRFPAFTAQ GESLVWHFSF FAADDRLAET LIAGKERFFL EHFIKSHSSN TEVFSERLLD LYARSYAKPH SLNASFEYYR ALNESVRQNA ELAKTRLQMP TMTLAGGGHG GMGTFQLEQM KAYADDVEGH VLPGCGHWLP EECAAPMNRL VIDFLSRGR

References (20)

Title : Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in Pseudomonas aeruginosa - He_2024_bioRxiv__
Author(s) : He S , Taher NM , Hvorecny KL , Ragusa MJ , Bahl CD , Hickman AB , Dyda F , Madden DR
Ref : Biorxiv , : , 2024
PubMedID: 38293063
Gene_locus related to this paper: pseae-PA2934

Title : Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase - Hvorecny_2017_Structure_25_697
Author(s) : Hvorecny KL , Bahl CD , Kitamura S , Lee KSS , Hammock BD , Morisseau C , Madden DR
Ref : Structure , 25 :697 , 2017
PubMedID: 28392259
Gene_locus related to this paper: pseae-PA2934

Title : Pseudomonas aeruginosa sabotages the generation of host proresolving lipid mediators - Flitter_2017_Proc.Natl.Acad.Sci.U.S.A_114_136
Author(s) : Flitter BA , Hvorecny KL , Ono E , Eddens T , Yang J , Kwak DH , Bahl CD , Hampton TH , Morisseau C , Hammock BD , Liu X , Lee JS , Kolls JK , Levy BD , Madden DR , Bomberger JM
Ref : Proc Natl Acad Sci U S A , 114 :136 , 2017
PubMedID: 27980032
Gene_locus related to this paper: pseae-PA2934

Title : Rational Design of Potent and Selective Inhibitors of an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa - Kitamura_2016_J.Med.Chem_59_4790
Author(s) : Kitamura S , Hvorecny KL , Niu J , Hammock BD , Madden DR , Morisseau C
Ref : Journal of Medicinal Chemistry , 59 :4790 , 2016
PubMedID: 27120257
Gene_locus related to this paper: pseae-PA2934

Title : Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif - Bahl_2016_Biochemistry_55_788
Author(s) : Bahl CD , Hvorecny KL , Morisseau C , Gerber SA , Madden DR
Ref : Biochemistry , 55 :788 , 2016
PubMedID: 26752215
Gene_locus related to this paper: pseae-PA2934

Title : Inhibiting an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa Protects CFTR - Bahl_2015_Angew.Chem.Int.Ed.Engl_54_9881
Author(s) : Bahl CD , Hvorecny KL , Bomberger JM , Stanton BA , Hammock BD , Morisseau C , Madden DR
Ref : Angew Chem Int Ed Engl , 54 :9881 , 2015
PubMedID: 26136396
Gene_locus related to this paper: pseae-PA2934

Title : Pseudomonas aeruginosa Cif Protein Enhances the Ubiquitination and Proteasomal Degradation of the Transporter Associated with Antigen Processing (TAP) and Reduces Major Histocompatibility Complex (MHC) Class I Antigen Presentation - Bomberger_2014_J.Biol.Chem_289_152
Author(s) : Bomberger JM , Ely KH , Bangia N , Ye S , Green KA , Green WR , Enelow RI , Stanton BA
Ref : Journal of Biological Chemistry , 289 :152 , 2014
PubMedID: 24247241
Gene_locus related to this paper: pseae-PA2934

Title : Signature motifs identify an acinetobacter cif virulence factor with epoxide hydrolase activity - Bahl_2014_J.Biol.Chem_289_7460
Author(s) : Bahl CD , Hvorecny KL , Bridges AA , Ballok AE , Bomberger JM , Cady KC , O'Toole GA , Madden DR
Ref : Journal of Biological Chemistry , 289 :7460 , 2014
PubMedID: 24474692
Gene_locus related to this paper: pseae-PA2934 , 9gamm-d0bwk6

Title : Epoxide-mediated CifR repression of cif gene expression utilizes two binding sites in Pseudomonas aeruginosa - Ballok_2012_J.Bacteriol_194_5315
Author(s) : Ballok AE , Bahl CD , Dolben EL , Lindsay AK , St Laurent JD , Hogan DA , Madden DR , O'Toole GA
Ref : Journal of Bacteriology , 194 :5315 , 2012
PubMedID: 22843844
Gene_locus related to this paper: pseae-PA2934

Title : Pseudomonas aeruginosa Cif defines a distinct class of alpha\/beta epoxide hydrolases utilizing a His\/Tyr ring-opening pair - Bahl_2012_Protein.Pept.Lett_19_186
Author(s) : Bahl CD , Madden DR
Ref : Protein Pept Lett , 19 :186 , 2012
PubMedID: 21933119
Gene_locus related to this paper: pseae-PA2934

Title : A Pseudomonas aeruginosa toxin that hijacks the host ubiquitin proteolytic system - Bomberger_2011_PLoS.Pathog_7_e1001325
Author(s) : Bomberger JM , Ye S , Maceachran DP , Koeppen K , Barnaby RL , O'Toole GA , Stanton BA
Ref : PLoS Pathog , 7 :e1001325 , 2011
PubMedID: 21455491
Gene_locus related to this paper: pseae-PA2934

Title : Crystal structure of the CFTR inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor. - Bahl_2010_J.Bacteriol_192_1785
Author(s) : Bahl CD , Morisseau C , Bomberger JM , Stanton BA , Hammock BD , O'Toole GA , Madden DR
Ref : Journal of Bacteriology , 192 :1785 , 2010
PubMedID: 20118260
Gene_locus related to this paper: pseae-PA2934

Title : Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa - Bahl_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_26
Author(s) : Bahl CD , Maceachran DP , O'Toole GA , Madden DR
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 66 :26 , 2010
PubMedID: 20057063
Gene_locus related to this paper: pseae-PA2934

Title : Chemotoxicity of doxorubicin and surface expression of P-glycoprotein (MDR1) is regulated by the Pseudomonas aeruginosa toxin Cif - Ye_2008_Am.J.Physiol.Cell.Physiol_295_C807
Author(s) : Ye S , Maceachran DP , Hamilton JW , O'Toole GA , Stanton BA
Ref : American Journal of Physiology Cell Physiol , 295 :C807 , 2008
PubMedID: 18650266
Gene_locus related to this paper: pseae-PA2934

Title : Cif is negatively regulated by the TetR family repressor CifR - MacEachran_2008_Infect.Immun_76_3197
Author(s) : Maceachran DP , Stanton BA , O'Toole GA
Ref : Infect Immun , 76 :3197 , 2008
PubMedID: 18458065
Gene_locus related to this paper: pseae-PA2934

Title : The Pseudomonas aeruginosa secreted protein PA2934 decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator - MacEachran_2007_Infect.Immun_75_3902
Author(s) : Maceachran DP , Ye S , Bomberger JM , Hogan DA , Swiatecka-Urban A , Stanton BA , O'Toole GA
Ref : Infect Immun , 75 :3902 , 2007
PubMedID: 17502391
Gene_locus related to this paper: pseae-PA2934

Title : Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen - Stover_2000_Nature_406_959
Author(s) : Stover CK , Pham XQ , Erwin AL , Mizoguchi SD , Warrener P , Hickey MJ , Brinkman FS , Hufnagle WO , Kowalik DJ , Lagrou M , Garber RL , Goltry L , Tolentino E , Westbrock-Wadman S , Yuan Y , Brody LL , Coulter SN , Folger KR , Kas A , Larbig K , Lim R , Smith K , Spencer D , Wong GK , Wu Z , Paulsen IT , Reizer J , Saier MH , Hancock RE , Lory S , Olson MV
Ref : Nature , 406 :959 , 2000
PubMedID: 10984043
Gene_locus related to this paper: pseae-clipa , pseae-CPO , pseae-metx , pseae-PA0201 , pseae-PA0231 , pseae-PA0308 , pseae-PA0368 , pseae-PA0480 , pseae-PA0502 , pseae-PA0543 , pseae-PA0599 , pseae-PA0829 , pseae-PA1166 , pseae-PA1211 , pseae-PA1239 , pseae-PA1291 , pseae-PA1304 , pseae-PA1510 , pseae-PA1558 , pseae-PA1597 , pseae-PA1621 , pseae-PA1622 , pseae-PA1680 , pseae-PA1771 , pseae-PA1888 , pseae-PA1907 , pseae-PA1990 , pseae-PA2086 , pseae-PA2098 , pseae-PA2168 , pseae-PA2302 , pseae-PA2411 , pseae-PA2425 , pseae-PA2451 , pseae-PA2540 , pseae-PA2682 , pseae-PA2689 , pseae-PA2745 , pseae-PA2764 , pseae-PA2927 , pseae-PA2934 , pseae-PA2949 , pseae-PA3053 , pseae-PA3132 , pseae-PA3226 , pseae-PA3301 , pseae-PA3324 , pseae-PA3327 , pseae-PA3429 , pseae-PA3509 , pseae-PA3586 , pseae-PA3628 , pseae-PA3695 , pseae-PA3734 , pseae-PA3829 , pseae-PA3859 , pseae-PA3994 , pseae-PA4008 , pseae-PA4152 , pseae-PA4440 , pseae-PA4968 , pseae-PA5080 , pseae-PA5384 , pseae-PA5513 , pseae-PCHC , pseae-PCHF , pseae-PHAC1 , pseae-PHAC2 , pseae-phaD , pseae-phag , pseae-PVDD , pseae-q9i4b9 , pseae-q9i538 , pseae-rhla , pseae-Y2218 , pseae-q9hyv3 , pseae-q9i252 , pseae-q9i6m9