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9zzzz-AncFT7

Synthetic construct of Renilla-type engineered ancestral luciferase variant (AncFT7)

Comment

Luciferase from Renilla reniformis (RLuc)<\/A> catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities

Relationship

Family : Haloalkane_dehalogenase-HLD2

Block : X

Position in NCBI Life Tree : synthetic construct

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> other sequences N E > artificial sequences N E > synthetic construct N E

Molecular evidence

No mutation

No kinetic

No disease

No inhibitor

Database

No Genbank

No Interpro

No Pfam

No UniProt

No PIRSF

No SUPERFAM

Sequence

Peptide

MVSASQRTTS TATGDEWWAK CKQVDVLDSE MSYYDSDPGK HKNTVIFLHG NPTSSYLWRN VIPHVEPLAR CLAPDLIGMG KSGKLPNHSY RFVDHYRYLS AWFDSVNLPE KVTIVCHDWG SGLGFHWCNE HRDRVKGIVH MESVVDVIES WDEWPDIEED IALIKSEAGE EMVLKKNFFI ERLLPSSIMR KLSEEEMDAY REPFVEPGES RRPTLTWPRE IPLVKGGKPD VIEIVKSYNK WLSTSKDIPK LFINADPGFF SNAIKKVTKN WPNQKTVTVK GLHFLQEDSP EEIGEAIADF LNELTK

References (1)

Title : Automated Engineering Protein Dynamics via Loop Grafting: Improving Renilla Luciferase Catalysis - Planas-Iglesias_2025_ACS.Catal_15_3391
Author(s) : Planas-Iglesias J , Majerova M , Pluskal D , Vasina M , Damborsky J , Prokop Z , Marek M , Bednar D
Ref : ACS Catal , 15 :3391 , 2025
Abstract :
PubMedSearch : Planas-Iglesias_2025_ACS.Catal_15_3391
PubMedID:
Gene_locus related to this paper: 9zzzz-AncFT7 , 9zzzz-AncHLDRLuc2