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9zzzz-AncHLDRLuc2

Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase with fragment transplabntation (Anc-FT) AncFT

Comment

Luciferase from Renilla reniformis (RLuc)<\/A> catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>) Preprint available of: Engineering Protein Dynamics of Ancestral Luciferase DOI: 10.26434\/chemrxiv.12808295

Relationship

Family : Haloalkane_dehalogenase-HLD2

Block : X

Position in NCBI Life Tree : synthetic construct

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> other sequences N E > artificial sequences N E > synthetic construct N E

Molecular evidence

Database

No Genbank

No Interpro

No Pfam

No UniProt

No PIRSF

No SUPERFAM

Sequence

Peptide

ATGDEWWAKC KQVDVLDSEM SYYDSDPGKH KNTVIFLHGN PTSSYLWRNV IPHVEPLARC LAPDLIGMGK SGKLPNHSYR FVDHYRYLSA WFDSVNLPEK VTIVCHDWGS GLGFHWCNEH RDRVKGIVHM ESVVDVIESW DEWPDIEEDI ALIKSEAGEE MVLKKNFFIE RLLPSSIMRK LSEEEMDAYR EPFVEPGESR RPTLTWPREI PIKGDGPEDV IEIVKSYNKW LSTSKDIPKL FINADPGFFS NAIKKVTKNW PNQKTVTVKG LHFLQEDSPE EIGEAIADFL NELT

References (4)

Title : Automated Engineering Protein Dynamics via Loop Grafting: Improving Renilla Luciferase Catalysis - Planas-Iglesias_2025_ACS.Catal_15_3391
Author(s) : Planas-Iglesias J , Majerova M , Pluskal D , Vasina M , Damborsky J , Prokop Z , Marek M , Bednar D
Ref : ACS Catal , 15 :3391 , 2025
Abstract :
PubMedSearch : Planas-Iglesias_2025_ACS.Catal_15_3391
PubMedID:
Gene_locus related to this paper: 9zzzz-AncFT7 , 9zzzz-AncHLDRLuc2

Title : Catalytic mechanism for Renilla-type luciferases - Schenkmayerova_2023_Nat.Catal_6_23
Author(s) : Schenkmayerova A , Toul M , Pluskal D , Baatallah R , Gagnot G , Pinto GP , Santana VT , Stuchla M , Neugebauer P , Chaiyen P , Damborsky J , Bednar D , Janin YL , Prokop Z , Marek M
Ref : Nature Catalysis , 6 :23 , 2023
Abstract :
PubMedSearch : Schenkmayerova_2023_Nat.Catal_6_23
PubMedID:
Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , renre-luc

Title : A catalytic mechanism for Renilla-type bioluminescence - Schenkmayerova_2022_Biorxiv__
Author(s) : Schenkmayerova A , Toul M , Pluskal D , Baatallah R , Gagnot G , Pinto GP , Santana VT , Stuchla M , Neugebauer P , Chaiyen P , Damborsky J , Bednar D , Janin YL , Prokop Z , Marek M
Ref : Biorxiv , : , 2022
Abstract :
PubMedSearch : Schenkmayerova_2022_Biorxiv__
PubMedID:
Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , renre-luc

Title : Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts - Chaloupkova_2019_ACS.Catal_9_48103
Author(s) : Chaloupkova, R , Liskova V , Tool M , Markova K , Sebestova E , Hernychova L , Marek M , Pinto GP , Pluskal D , Waterman J , Prokop Z , Damborsky J
Ref : ACS Catal , 9 :4810 , 2019
Abstract :
PubMedSearch : Chaloupkova_2019_ACS.Catal_9_48103
PubMedID:
Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , 9zzzz-AncHLDRLuc , renre-luc