Haloalkane_dehalogenase-HLD2
Relationship
Comment
Haloalkane dehalogenases (HLDs) are enzymes that catalyze the cleavage of carbon halogen bonds by a hydrolytic mechanism. The catalytic triad consists of the key nucleophile (Asp) the general base (His) and a catalytic acid (Asp or Glu). HLD where subdivided in three subfamily (Chovancova et al.) according to their substrate specificity and position of active site residues. Class one HLD1 (HLD-I) has catalytic triad Asp-His-Asp and halide-strabilizing residues Trp-Trp. Class two HLDII (HLD-II) has catalytic triad Asp-His-Glu and halide-stabilizing residues Asn-Trp. Class three HLDIII (HLD-III) has catalytic triad Asp-His-Asp and halide-strabilizing residues Asn-Trp. Although the reaction performed is different the Renilla reniformis luciferase belongs to this family. In ESTHER as in HAMAP database class I and III are merged as they are close. Luciferase from Renilla reniformis (RLuc) catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) (this luciferase is different from fireflies luciferases classified as Luciferin 4-monooxygenase; ATP-dependent AMP-binding enzyme family). Reconstruction of an ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.)
Database
Interpro : IPR023594 Haloalkane dehalogenase, subfamily 2
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF00561 Abhydrolase_1
Prints : No Print
EC Number : 3.8.1.5
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 1BN6 | Rhodococcus haloalkane dehalogenase | rhoso-halo1 |
| 1BN7 | Rhodococcus haloalkane dehalogenase in complex with acetate | rhoso-halo1 |
| 1CQW | Rhodococcus Sp. Haloalcane dehalogenase cocrystal Nai | rhoso-halo1 |
| 1CV2 | Hydrolytic haloalkane dehalogenase Linb from Sphingomonas paucimobilis UT26 at 1.6 A resolution | sphpi-linb |
| 1D07 | Hydrolytic haloalkane dehalogenase Linb from Sphingomonas paucimobilis Ut26 With 1,3-Propanediol, a product of debromidation of dibrompropane, at 2.0A Resolution | sphpi-linb |
| 8J1O | Crystal structure of HaloTag complexed with BTTA | rhoso-halo1 |
| 8B6N | X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta) | rhoso-halo1 |
| 8B6O | X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta) fused to M13 | rhoso-halo1 |
| 8B6P | X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 154-156 (cpHaloTag7_154-156) | rhoso-halo1 |
| 8B6Q | X-ray structure of the haloalkane dehalogenase HaloTag7 with an insertion of Calmodulin-M13 fusion at position 154-156 that mimic the structure of CaProLa, an calcium gated protein labeling technology | rhoso-halo1 |
| 8OE6 | Structure of hyperstable haloalkane dehalogenase variant DhaA231 | rhoso-halo1 |
| 8OE2 | Structure of hyperstable haloalkane dehalogenase variant DhaA223 | rhoso-halo1 |
| 8B5K | Structure of haloalkane dehalogenase DmmarA from Mycobacterium marinum at pH 6.5 | mycua-a0pum4 |
| 8B5O | Structure of haloalkane dehalogenase DmmarA from Mycobacterium marinum at pH 5.5 | mycua-a0pum4 |
| 7NFZ | Crystal structure of haloalkane dehalogenase LinB57 mutant (H272F) from Sphingobium japonicum UT26 | sphpi-linb |
| 6Y9E | Crystal structure of putative ancestral haloalkane dehalogenase AncHLD2 (node 2) | 9bact-AncHLD2 |
| 6Y9F | Crystal structure of putative ancestral haloalkane dehalogenase AncHLD3 (node 3) | 9bact-AncHLD3 |
| 7PW1 | Crystal structure of ancestral haloalkane dehalogenase AncLinB-DmbA | 9zzzz-AncLinB |
| 6Y9G | Crystal structure of putative ancestral haloalkane dehalogenase AncHLD5 (node 5) | 9bact-AncHLD5 |
| 6YN2 | Crystal structure of Renilla reniformis luciferase variant RLuc8-W121F\/E144Q in complex with a coelenteramide (the postcatalytic enzyme-product complex) | renre-luc |
| 6S6E | Crystal structure of the engineered ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc I161_F162PinsL) | 9zzzz-AncHLDRLuc |
| 7QXQ | Coelenteramide-bound Renilla-type luciferase (AncFT) | 9zzzz-AncHLDRLuc2 |
| 7QXR | Azacoelenterazine-bound Renilla-type luciferase (AncFT) | 9zzzz-AncHLDRLuc2 |
| 6S97 | Fragment transplantation onto a hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT) AncFT | 9zzzz-AncHLDRLuc2 |
| 7OMR | Crystal structure of coelenteramide-bound Renilla reniformis luciferase RLuc8-D162A variant | renre-luc |
| 7OMO | Crystal structure of coelenteramine-bound Renilla reniformis luciferase RLuc8-D120A variant with fully hydrolyzed product | renre-luc |
| 7OMD | Crystal structure of azacoelenterazine-bound Renilla reniformis luciferase variant RLuc8-D162A | renre-luc |
| 7OME | Azacoelenterazine-bound Renilla-type engineered ancestral luciferase variant (AncFT7) | 9zzzz-AncFT7 |
| 6G75 | Crystal structure of the common ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc) | 9zzzz-AncHLDRLuc |
| 4BRZ | Haloalkane dehalogenase from a Marine Rhodobacteraceae HanR | 9rhob-KF032932 |
| 4C6H | Haloalkane dehalogenase from a Marine Rhodobacteraceae in complex with 1-hexanol | 9rhob-KF032932 |
| 3WIB | Crystal structure of Y109W Mutant Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 | agrtu-DHAA |
| 3WI7 | Crystal Structure of the Novel Haloalkane Dehalogenase DatA from Agrobacterium tumefaciens C58 | agrtu-DHAA |
| 6S42 | The double mutant(Ile44Leu+Gln102His) of haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94 with an eliminated halide-binding site | brael-e2rv62 |
| 6XY9 | Crystal structure of haloalkane dehalogenase DbeA-M1 loop variant from Bradyrhizobium elkanii | brael-e2rv62 |
| 4K2A | Crystal structure of haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94 | brael-e2rv62 |
| 7O8B | Structure of haloalkane dehalogenase variant DhaA80 from Rhodococcus rhodochrous | rhoso-halo1 |
| 7O3O | Structure of haloalkane dehalogenase mutant DhaA80(T148L, G171Q, A172V, C176F) from Rhodococcus rhodochrous with ionic liquid | rhoso-halo1 |
| 8SW8 | Crystal Structure of HaloTag7 bound to JF669-HaloTag ligand | rhoso-halo1 |
| 8B6R | X-ray structure of the haloalkane dehalogenase HaloTag7 labeled with a chloroalkane Cyanine3 fluorophore substrate | rhoso-halo1 |
| 8B6S | X-ray structure of the haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG1) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 8B6T | X-ray structure of the interface optimized haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG5-TMR) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 7ZIV | X-ray structure of the haloalkane dehalogenase dead variant HaloTag7-D106A bound to a chloroalkane tetramethylrhodamine fluorophore ligand (CA-TMR) | rhoso-halo1 |
| 7ZIW | X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a butyltrifluoromethanesulfonamide tetramethylrhodamine ligand (TMR-T4) | rhoso-halo1 |
| 7ZIX | X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a butylmethanesulfonamide tetramethylrhodamine ligand (TMR-S4) | rhoso-halo1 |
| 7ZBD | Crystal structure of HaloTag complexed with TRaQ-G ligand | rhoso-halo1 |
| 7ZBB | Crystal structure of HaloTag complexed with TRaQ-G-ctrl ligand | rhoso-halo1 |
| 7ZBA | Crystal structure of HaloTag complexed with Me-TRaQ-G ligand | rhoso-halo1 |
| 7WAM | Crystal structure of HaloTag complexed with VL1 | rhoso-halo1 |
| 7WAN | Crystal structure of HaloTag complexed with UL2 | rhoso-halo1 |
| 7ZJ0 | X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a pentylmethanesulfonamide tetramethylrhodamine ligand (TMR-S5) | rhoso-halo1 |
| 7ZIZ | X-ray structure of the dead variant haloalkane dehalogenase HaloTag7-D106A bound to a pentanol tetramethylrhodamine ligand (TMR-Hy5) | rhoso-halo1 |
| 7ZIY | X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a pentyltrifluoromethanesulfonamide tetramethylrhodamine ligand (TMR-T5) | rhoso-halo1 |
| 7PCW | X-ray structure of Haloalkane Halotag7-M175W labeled with chloralkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 7PCX | X-ray structure of Haloalkane Halotag7-Q165W labeled with chloralkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 5VNP | X-ray crystal structure of Halotag bound to the P1 benzoxadiazole fluorogenic ligand | rhoso-halo1 |
| 7OO4 | HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye | rhoso-halo1 |
| 7OND | HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye | rhoso-halo1 |
| 7OIP | WITHDRAWN HaloTag Engineering for Enhanced Fluorogenicity with a Simple Channel Dye 1 | rhoso-halo1 |
| 7OIR | WITHDRAWN HaloTag Engineering for Enhanced Fluorogenicity with a Simple Channel Dye 2 | rhoso-halo1 |
| 6ZVU | X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-P174L labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6ZVV | X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-P174W labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6ZVW | X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6ZVX | X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H-P174L labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6ZVY | X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H-P174R labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6ZCC | X-Ray structure of the Haloalkane dehalogenase HOB (HaloTag7-based Oligonucleotide Binder) labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6Y7A | X-Ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate | rhoso-halo1 |
| 6Y7B | X-Ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-carbopyronine fluorophore substrate | rhoso-halo1 |
| 6TY7 | Crystal structure of haloalkane dehalogenase variant DhaA115 (synthetic construct) domain-swapped dimer type-1 (combined mutations of DhaA101 DhaA112 E20S\/F80R\/C128F\/T148L\/A155P\/A172I\/C176F\/D198W\/V219W\/C262L\/D266F) | rhoso-halo1 |
| 6XT8 | Crystal structure of haloalkane dehalogenase variant DhaA115 (synthetic construct) domain-swapped dimer type-2 (combined mutations of DhaA101 DhaA112 E20S\/F80R\/C128F\/T148L\/A155P\/A172I\/C176F\/D198W\/V219W\/C262L\/D266F) | rhoso-halo1 |
| 6XTC | Crystal structure of haloalkane dehalogenase variant DhaA177 domain-swapped dimer type-3 | rhoso-halo1 |
| 6SP8 | Structure of hyperstable haloalkane dehalogenase variant DhaA115 prepared by the 'soak-and-freeze' method under 150 bar of krypton pressure | rhoso-halo1 |
| 6SP5 | Structure of hyperstable haloalkane dehalogenase variant DhaA115 | rhoso-halo1 |
| 6U2M | Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635 | rhoso-halo1 |
| 6U32 | Crystal structure of HaloTag bound to tetramethylrhodamine-HaloTag ligand | rhoso-halo1 |
| 5Y2Y | Crystal structure of HaloTag (M175C) complexed with dansyl-PEG2-HaloTag ligand | rhoso-halo1 |
| 5Y2X | Crystal structure of apo-HaloTag (M175C) | rhoso-halo1 |
| 5UY1 | X-ray crystal structure of apo Halotag (Haloalkane dehalogenase) | rhoso-halo1 |
| 5UXZ | X-ray crystal structure of Halotag (Haloalkane dehalogenase) bound to the P9 benzothiadiazole fluorogenic ligand | rhoso-halo1 |
| 4KAJ | X-Ray Structure of the complex of Haloalkane dehalogenase HaloTag7 with HALTS, Northeast Structural Genomics Consortium (NESG) Target OR151 | rhoso-halo1 |
| 4KAA | Crystal structure of the halotag2 protein at the resolution 2.3A, Northeast Structural Genomics Consortium (NESG) target OR150 | rhoso-halo1 |
| 4KAC | X-Ray Structure of the complex HaloTag2 with HALTS. Northeast Structural Genomics Consortium (NESG) Target OR150. | rhoso-halo1 |
| 4KAF | Crystal Structure of Haloalkane dehalogenase HaloTag7 at the resolution 1.5A, Northeast Structural Genomics Consortium (NESG) Target OR151 | rhoso-halo1 |
| 4KYV | Crystal Structure of dehalogenase HaloTag2 with HALTS at the resolution 1.8A. Northeast Structural Genomics Consortium (NESG) Target OR150 | rhoso-halo1 |
| 6S06 | Crystal structure of haloalkane dehalogenase LinB D147C+L177C mutant (LinB73) from Sphingobium japonicum UT26 | sphpi-linb |
| 4WDQ | Crystal structure of haloalkane dehalogenase LinB32 mutant (L177W) from Sphingobium japonicum UT26 | sphpi-linb |
| 4WDR | Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26 | sphpi-linb |
| 5LKA | Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26 at 1.3 A resolution | sphpi-linb |
| 4H77 | Crystal structure of haloalkane dehalogenase LinB from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7D | Crystal structure of haloalkane dehalogenase LinB T81A mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7E | Crystal structure of haloalkane dehalogenase LinB V112A mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7F | Crystal structure of haloalkane dehalogenase LinB V134I mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7H | Crystal structure of haloalkane dehalogenase LinB T135A mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7I | Crystal structure of haloalkane dehalogenase LinB L138I mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7J | Crystal structure of haloalkane dehalogenase LinB H247A mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4H7K | Crystal structure of haloalkane dehalogenase LinB I253M mutant from Sphingobium sp. MI1205 | sphpi-q6vqx3 |
| 4WCV | Haloalkane dehalogenase DhaA mutant from Rhodococcus rhodochrous (T148L+G171Q+A172V+C176G) | rhoso-halo1 |
| 5FLK | Structure of haloalkane dehalogenase variant DhaA101 | rhoso-halo1 |
| 4FWB | Structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant DhaA31 in complex with 1, 2, 3 - trichloropropane SUPERSEDES 3RLT | rhoso-halo1 |
| 3U1T | Haloalkane Dehalogenase, DmmA, of marine microbial origin | 9cyan-q6dnd9 |
| 4F5Z | Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (L95V, A172V) | rhoso-halo1 |
| 4F60 | Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (T148L, G171Q, A172V, C176F) | rhoso-halo1 |
| 4E46 | Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA in complex with 2-propanol | rhoso-halo1 |
| 3SK0 | Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant DhaA12 | rhoso-halo1 |
| 3RK4 | Structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant DhaA31 | rhoso-halo1 |
| 3AFI | Crystal structure of DBJA (HIS-DBJA) Bradyrhizobium japonicum haloalkane dehalogenase | braja-dhaa |
| 3A2L | Crystal structure of DBJA (HIS-DBJA) Bradyrhizobium japonicum haloalkane dehalogenase (mutant dbja delta) | braja-dhaa |
| 3A2M | Crystal structure of DBJA (HIS-DBJA) Bradyrhizobium japonicum haloalkane dehalogenase (WILD TYPE Type I) | braja-dhaa |
| 3A2N | Crystal structure of DBJA (HIS-DBJA) Bradyrhizobium japonicum haloalkane dehalogenase (Wild Type Type II P21) | braja-dhaa |
| 3FWH | Structure of haloalkane dehalogenase mutant Dha15 (I135F\/C176Y) from Rhodococcus rhodochrous | rhoso-halo1 |
| 3G9X | Structure of haloalkane dehalogenase DhaA14 mutant I135F from Rhodococcus rhodochrous | rhoso-halo1 |
| 4HZG | Structure of haloalkane dehalogenase DhaA from Rhodococcus rhodochrous | rhoso-halo1 |
| 3FBW | Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant C176Y | rhoso-halo1 |
| 2PSD | Crystal Structure of the Luciferase from Renilla Reniformis A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (diammonium) | renre-luc |
| 2PSE | Crystal Structure of the Luciferase from Renilla Reniformis A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (KSCN) | renre-luc |
| 2PSF | Crystal Structure of the Luciferase from Renilla Reniformis A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (thiomaltoside) | renre-luc |
| 2PSH | Crystal Structure of the Luciferase from Renilla Reniformis T2A, K25A, A55T, C124A, S130A, K136R, A143M, M185V, M253L, E277A, S287L (PEG\/isopropanol) | renre-luc |
| 2PSJ | Crystal Structure of the Luciferase from Renilla Reniformis T2A, A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (PEG\/Isopropanol) | renre-luc |
| 2V9Z | Structure of the Rhodococcus haloalkane dehalogenase mutant with enhanced enantioselectivity | rhoso-halo1 |
| 2QVB | Crystal Structure of Haloalkane Dehalogenase Rv2579 from Mycobacterium tuberculosis | myctu-linb |
| 2O2H | Crystal structure of haloalkane dehalogenase Rv2579 from Mycobacterium tuberculosis complexed with 1,2-dichloroethane | myctu-linb |
| 2O2I | Crystal structure of haloalkane dehalogenase Rv2579 from Mycobacterium tuberculosis complexed with 1,3-propandiol | myctu-linb |
| 1G4H | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis complexed with Butan-1-Ol | sphpi-linb |
| 1G5F | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis Complexed With 1,2-Dichloroethane | sphpi-linb |
| 1G42 | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis Complexed With 1,2-Dichloropropane | sphpi-linb |
| 1IZ7 | Re-Refinement Of The Structure Of Hydrolytic Haloalkane Dehalogenase Linb From Sphingomonas Paucimobilis Ut26 At 1.6 A Resolution | sphpi-linb |
| 1IZ8 | Re-Refinement Of The Structure Of Hydrolytic Haloalkane Dehalogenase Linb From Sphingomonas Paucimobilis Ut26 With 1,3-Propanediol, A Product Of Debromidation Of Dibrompropane, At 2.0a Resolution | sphpi-linb |
| 1K5P | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis UT26 at 1.8A resolution | sphpi-linb |
| 1K6E | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis UT26 at 1.8A resolution with 1,2-propanediol (product of dehalogenation of 1,2-dibromopropane) | sphpi-linb |
| 1K63 | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis UT26 at 1.8A resolution with 2-bromo-2-propene-1-ol | sphpi-linb |
| 1MJ5 | Hydrolytic Haloalkane Dehalogenase Linb from Sphingomonas Paucimobilis UT26 at 1.8A resolution | sphpi-linb |
| 2BFN | crystal structure of the complex of the haloalkane dehalogenase LinB with the product of dehalogenation reaction 1,2-dichloropropane | sphpi-linb |
| Gene_locus | Name | Species |
|---|---|---|
| 9zzzz-AncLinB |  Ancestral Haloalkane Dehalogenase AncLinB-DmbA |
synthetic construct |
| brabe-DbbA | Branchiostoma belcheri (Amphioxus) Haloalkane dehalogenase HLD2 DbbA | Branchiostoma belcheri |
| strpu-DspB | Strongylocentrotus purpuratus (Purple sea urchin) Haloalkane dehalogenase HLD2 DspB | Strongylocentrotus purpuratus |
| sacko-DskA | Saccoglossus kowalevskii Haloalkane dehalogenase HLD2 DskA | Saccoglossus kowalevskii |
| capte-r7umg5 | Capitella teleta (Polychaete worm) Haloalkane dehalogenase HLD2 DctA | Capitella teleta |
| 9arch-a0a1q9njs0 | Candidatus Heimdallarchaeota archaeon LC_3 Haloalkane dehalogenase HLD2 dhaA DchA | Candidatus Heimdallarchaeota archaeon LC_3 |
| 9noca-DrxA | Rhodococcus sp. EPR-157 Haloalkane dehalogenase HLD2 DrxA | Rhodococcus sp. EPR-157 |
| sphsx-DspxA | unclassified Sphingomonas Haloalkane dehalogenase HLD2 DspxA | unclassified Sphingomonas |
| 9noca-k0eup9 | Nocardia brasiliensis ATCC 700358 Haloalkane dehalogenase HLD2 DnbA | Nocardia brasiliensis ATCC 700358 |
| triha-a0a0f9y4y5 | Trichoderma harzianum (Hypocrea lixii) Haloalkane dehalogenase HLD2 DthB | Trichoderma harzianum |
| 9rhob-a0a0n7m2p6 | Phaeobacter sp. CECT 5382 Haloalkane dehalogenase HLD2 linB DphxA | Phaeobacter sp. CECT 5382 |
| triha-a0a0f9x982 | Trichoderma harzianum (Hypocrea lixii) Haloalkane dehalogenase HLD2 DthA | Trichoderma harzianum |
| rhile-DrgA | Rhizobium leguminosarum Haloalkane dehalogenase HLD2 DrgA | Rhizobium leguminosarum |
| ensad-DeaA | Ensifer adhaerens (Sinorhizobium morelense) Haloalkane dehalogenase HLD2 DeaA | Ensifer adhaerens |
| 9actn-a0a0c1uk61 | marine actinobacterium MedAcidi-G3 Haloalkane dehalogenase HLD2 DmaA | marine actinobacterium MedAcidi-G3 |
| 9pseu-DathA | Amycolatopsis thermoflava Haloalkane dehalogenase HLD2 DathA | Amycolatopsis thermoflava |
| amys7-DaxA | Amycolatopsis sp. (strain ATCC 39116 \/ 75iv2) Haloalkane dehalogenase HLD2 DaxA | Amycolatopsis sp. ATCC 39116 |
| lenae-DlaA | Lentzea aerocolonigenes (Lechevalieria aerocolonigenes) (Saccharothrix aerocolonigenes) Haloalkane dehalogenase HLD2 DlaA | Lentzea aerocolonigenes |
| 9rhob-DpxA | Pseudovibrio sp. POLY-S9 Haloalkane dehalogenase HLD2 DpxA | Pseudovibrio sp. POLY-S9 |
| 9actn-a0a1s1sm32 | Frankia sp. BMG5.36 Haloalkane dehalogenase HLD2 DfxA | Frankia sp. BMG5.36 |
| 9bact-AncHLD3 | Putative ancestral haloalkane dehalogenase AncHLD3 (node 3) |
uncultured bacterium |
| 9bact-AncHLD2 | Putative ancestral haloalkane dehalogenase AncHLD2 (node 2) |
uncultured bacterium |
| 9bact-AncHLD5 | Putative ancestral haloalkane dehalogenase AncHLD5 (node 5) |
uncultured bacterium |
| 9zzzz-AncFT7 | Synthetic construct of Renilla-type engineered ancestral luciferase variant (AncFT7) |
synthetic construct |
| 9zzzz-AncHLDRLuc2 | Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase with fragment transplabntation (Anc-FT) AncFT |
synthetic construct |
| 9zzzz-AncHLDRLuc | Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase |
synthetic construct |
| 9rhob-KF032932 | Rhodobacteraceae bacterium Haloalkane_dehalogenase HanR |
Rhodobacteraceae bacterium UDC319 |
| 9bact-b4dav5 | Chthoniobacter flavus Ellin428 Haloalkane dehalogenase | Chthoniobacter flavus Ellin428 |
| 9bact-q6sht4 | uncultured bacterium 311 haloalkane dehalogenase HLD2 DmbaA | uncultured bacterium 311 |
| 9burk-b5whg4 | Burkholderia sp. H160 Haloalkane dehalogenase | Burkholderia sp. H160 |
| 9cyan-q6dnd9 | Lyngbya majuscula curN, DmmA, a haloalkane dehalogenase |
Lyngbya majuscula |
| 9rhob-a3sf89 | Sulfitobacter sp. EE-36\; Sulfitobacter pontiacus\; (1-chlorohexanehalidohydrolase) Haloalkane dehalogenase 2 DhaA DspoA | Sulfitobacter sp. EE-36 |
| 9rhob-a4etx5 | Roseobacter sp. SK209-2-6 Haloalkane dehalogenase | Roseobacter sp. SK209-2-6 |
| agrtu-DHAA | Agrobacterium tumefaciens, Agrobacterium radiobacter, haloalkane dehalogenase DatA |
Agrobacterium tumefaciens |
| brael-e2rv62 | Bradyrhizobium elkanii haloalkane dehalogenase (EC 3.8.1.5) DbeA |
Bradyrhizobium elkanii |
| brafl-c3xxt9 | Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein | Branchiostoma floridae |
| braja-dhaa | Bradyrhizobium japonicum\; Bradyrhizobium diazoefficiens, haloalkane dehalogenase (EC 3.8.1.5) DhaA DbjA |
Bradyrhizobium japonicum |
| deidv-c1cwe6 | Deinococcus deserti (strain VCD115 \/ DSM 17065 \/ LMG 22923) Haloalkane dehalogenase | Deinococcus deserti |
| desps-q6ajw5 | Desulfotalea psychrophila related to haloalkane dehalogenase HLDIII DdpA | Desulfotalea psychrophila |
| geosf-b9m8q3 | Geobacter sp. (strain FRC-32) Alpha\/beta hydrolase fold protein | Geobacter sp. |
| rhilo-dhaa | Mesorhizobium loti (Rhizobium loti), Mesorhizobium huakuii DHAA mlr5354 haloalkane dehalogenase | Mesorhizobium loti |
| mycmm-b2hjb4 | Mycobacterium marinum, Mycobacterium liflandii, Haloalkane dehalogenase 2 DhaA_1 DmmarB | Mycobacterium marinum |
| mycsm-Q938B4 | Mycobacterium smegmatis putative linb haloalkane dehalogenase (fragment) | Mycobacterium smegmatis |
| myctu-linb | Mycobacterium tuberculosis, Mycobacterium bovis, DHAA linb DhaA dmbA DmbA Rv2296 haloalkane dehalogenase (EC 3.8.1.5) |
Mycobacterium tuberculosis |
| mycua-a0pum4 | Mycobacterium ulcerans (strain Agy99) Mycobacterium marinum (strain ATCC BAA-535 \/ M) Haloalkane dehalogenase 2 DhaA DmmarA DmuA |
Mycobacterium ulcerans |
| renmu-Q9BLZ3 | Renilla muelleri epoxide hydrolase (EC 3.3.2.3) | Renilla muelleri |
| renre-luc | Renilla reniformis luciferase , luciferin 2-monooxygenase |
Renilla reniformis |
| rhoso-halo1 | Rhodococcus rhodochrous (Pseudomonas pavonaceae), Rhodococcus sp., Mycobacterium sp haloalkane dehalogenase (EC 3.8.1.5) DhaA HaloTag (hyperstable variant DhaA115) |
Rhodococcus rhodochrous |
| sphpi-linb | Sphingomonas paucimobilis (Pseudomonas paucimobilis)\; Sphingomonas sp., Sphingobium (japonicum ummariense indicum), Pseudomonas aeruginosa, LinB |
Sphingomonas paucimobilis |
| sphpi-q6vqx3 | Sphingomonas paucimobilis (Pseudomonas paucimobilis)\; Sphingobium sp., Sphingobium indicum\; Haloalkane dehalogenase linB gene |
Sphingomonas paucimobilis |
| strpu-h3hrw2 | Strongylocentrotus purpuratus (purple sea urchin) Haloalkane_dehalogenase | Strongylocentrotus purpuratus |
| triad-b3s949 | Trichoplax adhaerens (Trichoplax reptans) Putative uncharacterized protein | Trichoplax adhaerens |
| triad-b3s950 | Trichoplax adhaerens (Trichoplax reptans) Putative uncharacterized protein | Trichoplax adhaerens |
| 9chlr-d6tri7 | Ktedonobacter racemifer DSM 44963 Haloalkane dehalogenase | Ktedonobacter racemifer DSM 44963 |
| 9chlr-d6tuz4 | Ktedonobacter racemifer DSM 44963 Haloalkane dehalogenase | Ktedonobacter racemifer DSM 44963 |
| mescw-e8t767 | Mesorhizobium ciceri bv. biserrulae (strain HAMBI 2942 \/ LMG 23838 \/ WSM1271) Haloalkane dehalogenase | Mesorhizobium ciceri bv. biserrulae |
| brajp-g7dmu1 | Bradyrhizobium japonicum Haloalkane dehalogenase | Bradyrhizobium japonicum USDA 6 |
| brajp-g7dnh7 | Bradyrhizobium japonicum USDA 6 Haloalkane dehalogenase | Bradyrhizobium japonicum USDA 6 |
| hypvg-g9my22 | Hypocrea virens (strain Gv29-8 \/ FGSC 10586) (Gliocladium virens)(Trichoderma virens) Uncharacterized protein | Hypocrea virens |
| hypai-g9p2e2 | Hypocrea atroviridis (strain ATCC 20476 \/ IMI 206040) (Trichoderma atroviride) Putative Haloalkane dehalogenase | Hypocrea atroviridis |
| 9brad-h0tue9 | Bradyrhizobium sp. STM 3843 Haloalkane dehalogenase | Bradyrhizobium sp. STM 3843 |
| 9brad-i2qj82 | Bradyrhizobium sp. WSM1253 Haloalkane dehalogenase | Bradyrhizobium sp. WSM1253 |
| 9rhiz-j2kud1 | Rhizobium sp. CF142 Haloalkane dehalogenase | Rhizobium sp. CF142 |
| mycsm-l0iu38 | Mycobacterium smegmatis JS623 Haloalkane dehalogenase | Mycobacterium smegmatis JS623 |
| myxsd-l7uf81 | Myxococcus stipitatus (strain DSM 14675 \/ JCM 12634 \/ Mx s8) Haloalkane dehalogenase | Myxococcus stipitatus |
| 9brad-u1imq3 | Bradyrhizobium sp. DFCI-1 Haloalkane dehalogenase | Bradyrhizobium sp. DFCI-1 |
| 9bact-v5llz5 | uncultured bacterium Haloalkane dehalogenase Cluster C eHLD-C | uncultured bacterium |
| mycne-v5x835 | Mycobacterium neoaurum VKM Ac-1815D Haloalkane dehalogenase | Mycobacterium neoaurum VKM Ac-1815D |
| 9rhiz-v7fq40 | Mesorhizobium sp. LSHC420B00 Haloalkane dehalogenase | Mesorhizobium sp. LSHC420B00 |
| 9delt-w4lkq9 | Candidatus Entotheonella sp. Haloalkane dehalogenase | Candidatus Entotheonella sp. TSY1 |
| 9burk-w4p0d0 | Burkholderia caribensis MBA4 Haloalkane dehalogenase | Burkholderia caribensis MBA4 |
| 9pseu-w7svw4 | Kutzneria sp. 744 Haloalkane dehalogenase | Kutzneria sp. 744 |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| BTTA | N-[[1-[2-[2-(2-hexoxyethoxy)ethoxy]ethyl]-1,2,3-triazol-4-yl]methyl]-1-(1H-1,2,3-triazol-4-yl)-N-(2H-1,2,3-triazol-4-ylmethyl)methanamine | rhoso-halo1 |
| Chloroalkane-Cyanine3-fluorophore | ~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-6-[3,3-dimethyl-2-[(~{E})-3-(1,3,3-trimethylindol-2-ylidene)prop-1-enyl]indol-1-ium-1-yl]hexanamide | rhoso-halo1 |
| JF669-HaloTag | 2-[3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-benzo[b][1]benzosilin-10-yl]-3,5,6-tris(fluoranyl)-4-[2-(2-hexoxyethoxy)ethylcarbamoyl]benzoic acid | rhoso-halo1 |
| TMR-S4 | [9-[2-carboxy-5-[2-[2-[4-(methylsulfonylamino)butoxy]ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium | rhoso-halo1 |
| TMR-T4 | [9-[2-carboxy-5-[2-[2-[4-(trifluoromethylsulfonylamino)butoxy]ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium | rhoso-halo1 |
| TMR-S5 | [9-[2-carboxy-5-[2-[2-[5-(methylsulfonylamino)pentoxy]ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium | rhoso-halo1 |
| TMR-Hy5 | [9-[2-carboxy-5-[2-[2-(5-oxidanylpentoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium | rhoso-halo1 |
| TMR-T5 | [9-[2-carboxy-5-[2-[2-[5-(trifluoromethylsulfonylamino)pentoxy]ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium | rhoso-halo1 |
| HyT13 | 2-(1-adamantyl)-N-[2-[2-(6-chlorohexoxy)ethoxy]ethyl]acetamide | rhoso-halo1 |
| Azacoelenterazine | 8-benzyl-6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-[1,2,4]triazolo[4,3-a]pyrazin-3-one | 9zzzz-AncHLDRLuc2 renre-luc 9zzzz-AncHLDRLuc |
| TRaQ-G-ILQ | (10~{R})-7-azanyl-~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-2'-cyano-5,5-dimethyl-3-(methylamino)-1'-oxidanylidene-spiro[benzo[b][1]benzosiline-10,3'-isoindole]-5'-carboxamide | rhoso-halo1 |
| TRaQ-G-ILU | [7-azanyl-10-[5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]-2-(cyanocarbamoyl)phenyl]-5,5-dimethyl-benzo[b][1]benzosilin-3-ylidene]-methyl-azanium | rhoso-halo1 |
| TRaQ-G-ctrl | (E)-[7-azanyl-10-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-5,5-dimethyl-benzo[b][1]benzosilin-3-ylidene]-methyl-azanium | rhoso-halo1 |
| Me-TRaQ-G | 4-(7-azanyl-5,5-dimethyl-3-methylimino-benzo[b][1]benzosilin-10-yl)-~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-3-methyl-benzamide | rhoso-halo1 |
| VL1-8MH | 3-[6-(2-azanylhydrazinyl)-1,3-bis(oxidanylidene)benzo[de]isoquinolin-2-yl]-N-[2-(2-hexoxyethoxy)ethyl]propanamide | rhoso-halo1 |
| UL2-8MS | (R)-[4-(2-azanylhydrazinyl)phenyl]-[2-[2-(2-hexoxyethoxy)ethoxy]ethylamino]methanol | rhoso-halo1 |
| P1-benzoxadiazole-fluorogenic-ligand | N-[2-(2-hexoxyethoxy)ethyl]-2-[methyl-[[7-(methylamino)-2,1,3-benzoxadiazol-4-yl]sulfonyl]amino]acetamide | rhoso-halo1 |
| VF2-Styrylpyridine-Dye | 4-[2-[1-(7-chloroheptyl)pyridin-1-ium-4-yl]ethenyl]-N,N-dimethylaniline | rhoso-halo1 |
| Coelenteramine | 4-(5-amino-6-benzylpyrazin-2-yl)phenol | renre-luc |
| Coelenteramide | N-[3-benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl]-2-(4-hydroxyphenyl)acetamide | renre-luc |
| Chloroalkane-carbopyronine-fluorophore | [10-[2-carboxy-5-[2-[2-(6-chlorohexoxy)ethoxy]ethylcarbamoyl]phenyl]-7-(dimethylamino)-9,9-dimethylanthracen-2-ylidene]-dimethylazanium | rhoso-halo1 |
| Chloroalkane-tetramethylrhodamine-fluorophore | [9-[2-carboxy-5-[2-[2-(6-chlorohexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium | rhoso-halo1 |
| tetramethylrhodamine-HaloTag-ligand | [9-[2-carboxy-5-[2-(2-hexoxyethoxy)ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium | rhoso-halo1 |
| JF635i | rhoso-halo1 | |
| JF635 | N-[2-[2-(6-chlorohexoxy)ethoxy]ethyl]-3',7'-bis(3-fluoroazetidin-1-yl)-5',5'-dimethyl-1-oxospiro[2-benzofuran-3,10'-benzo[b][1]benzosiline]-5-carboxamide | rhoso-halo1 |
| CHES | 2-(cyclohexylamino)ethanesulfonic acid | agrtu-DHAA |
| dansyl-PEG2-HaloTag | 5-(dimethylamino)-~{N}-[2-(2-hexoxyethoxy)ethyl]naphthalene-1-sulfonamide | rhoso-halo1 |
| P9-benzothiadiazole | 2-[[7-(dimethylamino)-2,1,3-benzothiadiazol-4-yl]sulfonyl-methylamino]-N-(6-hydroxyhexyl)acetamide | rhoso-halo1 |
| HALSTS-1Q9 | N-[(2-ethoxy-3,5-dimethylphenyl)methyl]-2H-tetrazol-5-amine | rhoso-halo1 |
| HALSTS1 | N-[(3,5-dichloro-2-ethoxyphenyl)methyl]-2H-tetrazol-5-amine | rhoso-halo1 |
| Butan-1-Ol | butan-1-ol | |
| Benzoic-acid | benzoic acid | sorbi-hnl strau-cpoT psefl-cumD rhosm-cocE rhoso-halo1 idesa-mheth hevbr-hnl |
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| Gene_locus related to this paper: renre-luc |
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| Gene_locus related to this paper: sphpi-linb |
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| Abstract : |
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| Gene_locus related to this paper: sphpi-linb |
| Title : Haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26: X-ray crystallographic studies of dehalogenation of brominated substrates - Streltsov_2003_Biochemistry_42_10104 |
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| Abstract : |
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| Gene_locus related to this paper: sphpi-linb |
| Title : Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition - Oakley_2002_Biochemistry_41_4847 |
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| Abstract : |
| PubMedSearch : Oakley_2002_Biochemistry_41_4847 |
| PubMedID: 11939779 |
| Gene_locus related to this paper: sphpi-linb |
| Title : Crystal structure of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26 - Marek_2000_Biochemistry_39_14082 |
| Author(s) : Marek J , Vevodova J , Smatanova IK , Nagata Y , Svensson LA , Newman J , Takagi M , Damborsky J |
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| Abstract : |
| PubMedSearch : Marek_2000_Biochemistry_39_14082 |
| PubMedID: 11087355 |
| Gene_locus related to this paper: sphpi-linb |
| Title : Haloalkane dehalogenases: structure of a Rhodococcus enzyme - Newman_1999_Biochemistry_38_16105 |
| Author(s) : Newman J , Peat TS , Richard R , Kan L , Swanson PE , Affholter JA , Holmes IH , Schindler JF , Unkefer CJ , Terwilliger TC |
| Ref : Biochemistry , 38 :16105 , 1999 |
| Abstract : |
| PubMedSearch : Newman_1999_Biochemistry_38_16105 |
| PubMedID: 10587433 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Repositioning the catalytic triad aspartic acid of haloalkane dehalogenase: effects on stability, kinetics, and structure - Krooshof_1997_Biochemistry_36_9571 |
| Author(s) : Krooshof GH , Kwant EM , Damborsky J , Koca J , Janssen DB |
| Ref : Biochemistry , 36 :9571 , 1997 |
| Abstract : |
| PubMedSearch : Krooshof_1997_Biochemistry_36_9571 |
| PubMedID: 9236003 |