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9zzzz-AncHLDRLuc

Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase

Comment

Luciferase from Renilla reniformis (RLuc) catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>)

Relationship

Family : Haloalkane_dehalogenase-HLD2

Block : X

Position in NCBI Life Tree : synthetic construct

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> other sequences N E > artificial sequences N E > synthetic construct N E

Molecular evidence

Database

No Genbank

No Interpro

No Pfam

No UniProt

No PIRSF

No SUPERFAM

Sequence

Peptide

ATGDEWWAKC KQVDVLDSEM SYYDSDPGKH KNTVIFLHGN PTSSYLWRNV IPHVEPLARC LAPDLIGMGK SGKLPNHSYR FVDHYRYLSA WFDSVNLPEK VTIVCHDWGS GLGFHWCNEH RDRVKGIVHM ESVVSPLKGW ESFPETARDI FQALRSEAGE EMVLKKNFFI ERLLPSSIMR KLSEEEMDAY REPFVEPGES RRPTLTWPRE IPIKGDGPED VIEIVKSYNK WLSTSKDIPK LFINADPGFF SNAIKKVTKN WPNQKTVTVK GLHFLQEDSP EEIGEAIADF LNELTK

References (1)

Title : Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts - Chaloupkova_2019_ACS.Catal_9_48103
Author(s) : Chaloupkova, R , Liskova V , Tool M , Markova K , Sebestova E , Hernychova L , Marek M , Pinto GP , Pluskal D , Waterman J , Prokop Z , Damborsky J
Ref : ACS Catal , 9 :4810 , 2019
Abstract :
PubMedSearch : Chaloupkova_2019_ACS.Catal_9_48103
PubMedID:
Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , 9zzzz-AncHLDRLuc , renre-luc