ecoli-T6SS.TLE3

Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435

Comment

Family of Tle3 phospholipase effector .The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 (T6SS-TLE1) family, TLE2 to Lipase_3, TLE3 to alpha\/ beta hydrolase and Tle4 belongs to PGAP1 (Tle5 family, such as Pseudomonas aeruginosa PldA and PldB, are phospholipases D and are not alpha\/beta hydrolases). Tle3 phospholipase effector from adherent-invasive Escherichia coli (AIEC) Tle3AIEC possesses phospholipase A1 (PLA1) activity (specific activity (SA) = 7.65 nmole.min-1.mg-1) but no PLA2 activity. . The T6SS-TLE3 is decorated by two protruding segments, including a N-terminus loop containing part of the oxyanion hole residues. The long C-terminus contains the catalytic site histidine and in some members is related to the DUF3274 family (most members of this family are not linked to alpha\/beta hydrolases).In T6SS, protection against its own toxic effector is ensured in bacteria by the production of immunity proteins that specifically bind and inhibit Tle3. Tli (Type VI lipase immunity) binds tightly to Tle3. There is also a strong interaction between the VgrGAIEC C-terminus adaptor and Tle3AIEC N-terminal loop. Other strains: Escherichia coli O83:H1 (strain NRG 857C \/ AIEC)\; Escherichia coli O6:H1 (strain CFT073 \/ ATCC 700928 \/ UPEC)

Relationship

Family : T6SS-TLE3

Block : No block

Position in NCBI Life Tree : Escherichia coli

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Proteobacteria N E > Gammaproteobacteria N E > Enterobacterales N E > Enterobacteriaceae N E > Escherichia N E > Escherichia coli N E

Molecular evidence

No mutation

No kinetic

No disease

No inhibitor

Sequence

Peptide

MSTNKSEPTR KVDVHLTDNG TPFAYSMTSH KNVKVRAEVQ PPLQLPGLII FVHGVNSEGE WYDYAERSLC AGLNQRLGLE GEHGLKENNY EGGFFVNSDK SEGGWEHTYE IEGSQKKWVS GPRKITKGGD GRSPVIRFYW GYRAADNETD TYAIPLKNKK GDNYYDLPPE SRKAKGPWFW GGGPFQNGCN QLVSLWSKTG FNNNPSLLGV PLPFSTQVLN GERDRLLSDA PPRHYYAHAA GRLAKLIKTI RNQHPEDTVT VLSHSQGTMI ALAAAAIEAP DALFVMNSPY ALENEPTTYI SYPIKEIISR KARSATFADI VKKVAENKTR LKQQGCDNLL AGMSSDGNSW IPEGKTHNGL PERDNHGTTW IYCNPHDRVM GSSPLRSIGW QGLPDTKDGT PHTLFKQAGD TLYVRILGRN TPCGGTPTAQ THFSNLGDGK PFWDSTTTLL QRATWPDPDS GQTLTINAPQ VPEPLTAEEL KNFDQDYARD EKQSGGAGYA YGQINPETKK PVDTDYRYYI SLYGYFDRKM VPKKDSGYYQ SGPGSKEDRV KYEKQSQEEM LEEVRTYVQR PTDHSTLPSD ERFMSRVVAY DLPIGYCWHS WDKAGLEELR RQADWLESDD YYFSGKLTVP PIPPAIKQDV AEDAEQRKAE EKARLRNV

References (2)

Title : Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS Effector\/Immunity Complex Determined Using an AlphaFold2 Predicted Model - Le_2023_Int.J.Mol.Sci_24_1740
Author(s) : Le TTH , Kellenberger C , Boyer M , Santucci P , Flaugnatti N , Cascales E , Roussel A , Canaan S , Journet L , Cambillau C
Ref : Int J Mol Sci , 24 :1740 , 2023
PubMedID: 36675258
Gene_locus related to this paper: ecoli-T6SS.TLE3

Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508
Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD
Ref : Nature , 496 :508 , 2013
PubMedID: 23552891
Gene_locus related to this paper: ecoli-T6SS.TLE3