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T6SS-TLE3

Relationship

Family: T6SS-TLE3

Block: X

Parent Family: No family

Comment

Family of Tle3 phospholipase effector .The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 family (now T6SS-TLE1), TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase (now T6SS-TLE3) and Tle4 belongs to PGAP1 (Tle5 family, such as Pseudomonas aeruginosa PldA and PldB, are phospholipases D and are not alpha/beta hydrolases). Tle3 phospholipase effector from adherent-invasive Escherichia coli (AIEC) Tle3AIEC possesses phospholipase A1 (PLA1) activity (specific activity (SA) = 7.65 nmole.min-1.mg-1) but no PLA2 activity. The T6SS-TLE3 is decorated by two protruding segments, including a N-terminus loop containing part of the oxyanion hole residues. The long C-terminus contains the catalytic site histidine and in some members is related to the DUF3274 family (most members of this family are not linked to alpha/beta hydrolases). In T6SS, protection against its own toxic effector is ensured in bacteria by the production of immunity proteins that specifically bind and inhibit Tle3. Tli (Type VI lipase immunity) binds tightly to Tle3. There is also a strong interaction between the VgrGAIEC C-terminus adaptor and Tle3AIEC N-terminal loop.

Database

Interpro : No interpro

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
8BOZ Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS effector\/immunity complex ecoli-T6SS.TLE3
8BOZ Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS effector\/immunity complex ecoli-T6SS.TLE3
8BOZ Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS effector\/immunity complex ecoli-T6SS.TLE3

Gene_locus Name Species
9burk-a0a069pb40 Caballeronia glathei. DUF3274 domain-containing protein Caballeronia glathei
9burk-a0a069pb40 Caballeronia glathei. DUF3274 domain-containing protein Caballeronia glathei
9burk-a0a069pb40 Caballeronia glathei. DUF3274 domain-containing protein Caballeronia glathei
9burk-a0a0q5nyl5 Burkholderia sp. Leaf177. DUF3274 domain-containing protein Burkholderia sp. Leaf177
9burk-a0a0q5nyl5 Burkholderia sp. Leaf177. DUF3274 domain-containing protein Burkholderia sp. Leaf177
9burk-a0a0q5nyl5 Burkholderia sp. Leaf177. DUF3274 domain-containing protein Burkholderia sp. Leaf177
9burk-a0a126zda3 Variovorax sp. PAMC 28711. Transmembrane protein Variovorax sp. PAMC 28711
9burk-a0a126zda3 Variovorax sp. PAMC 28711. Transmembrane protein Variovorax sp. PAMC 28711
9burk-a0a126zda3 Variovorax sp. PAMC 28711. Transmembrane protein Variovorax sp. PAMC 28711
9burk-a0a1i4t633 Rugamonas rubra. DUF3274 domain-containing protein Rugamonas rubra
9burk-a0a1i4t633 Rugamonas rubra. DUF3274 domain-containing protein Rugamonas rubra
9burk-a0a1i4t633 Rugamonas rubra. DUF3274 domain-containing protein Rugamonas rubra
9burk-a0a1i4u0b9 Rugamonas rubra. DUF3274 domain-containing protein Rugamonas rubra
9burk-a0a1i4u0b9 Rugamonas rubra. DUF3274 domain-containing protein Rugamonas rubra
9burk-a0a1i4u0b9 Rugamonas rubra. DUF3274 domain-containing protein Rugamonas rubra
9burk-a0a1p8jq21 Rhodoferax koreense. DUF3274 domain-containing protein Rhodoferax koreense
9burk-a0a1p8jq21 Rhodoferax koreense. DUF3274 domain-containing protein Rhodoferax koreense
9burk-a0a1p8jq21 Rhodoferax koreense. DUF3274 domain-containing protein Rhodoferax koreense
9burk-a0a1q8iqv1 Burkholderia sp. SRS-W-2-2016. DUF3274 domain-containing protein Burkholderia sp. SRS-W-2-2016
9burk-a0a1q8iqv1 Burkholderia sp. SRS-W-2-2016. DUF3274 domain-containing protein Burkholderia sp. SRS-W-2-2016
9burk-a0a1q8iqv1 Burkholderia sp. SRS-W-2-2016. DUF3274 domain-containing protein Burkholderia sp. SRS-W-2-2016
9burk-a0a2r4c9j8 Pseudoduganella armeniaca. Transmembrane protein Pseudoduganella armeniaca
9burk-a0a2r4c9j8 Pseudoduganella armeniaca. Transmembrane protein Pseudoduganella armeniaca
9burk-a0a2r4c9j8 Pseudoduganella armeniaca. Transmembrane protein Pseudoduganella armeniaca
9burk-a0a3d8k099 Trinickia dinghuensis. Transmembrane protein Trinickia dinghuensis
9burk-a0a3d8k099 Trinickia dinghuensis. Transmembrane protein Trinickia dinghuensis
9burk-a0a3d8k099 Trinickia dinghuensis. Transmembrane protein Trinickia dinghuensis
9burk-a0a7z2zsr3 Massilia forsythiae. Transmembrane protein Massilia forsythiae
9burk-a0a7z2zsr3 Massilia forsythiae. Transmembrane protein Massilia forsythiae
9burk-a0a7z2zsr3 Massilia forsythiae. Transmembrane protein Massilia forsythiae
9burk-w0vf87 Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628. DUF3274 domain-containing protein Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628
9burk-w0vf87 Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628. DUF3274 domain-containing protein Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628
9burk-w0vf87 Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628. DUF3274 domain-containing protein Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628
9entr-a0a089px10 Cedecea neteri. DUF3274 domain-containing protein Cedecea neteri
9entr-a0a089px10 Cedecea neteri. DUF3274 domain-containing protein Cedecea neteri
9entr-a0a089px10 Cedecea neteri. DUF3274 domain-containing protein Cedecea neteri
9entr-a0a2j0pr84 Enterobacter kobei. DUF3274 domain-containing protein Enterobacter kobei
9entr-a0a2j0pr84 Enterobacter kobei. DUF3274 domain-containing protein Enterobacter kobei
9entr-a0a2j0pr84 Enterobacter kobei. DUF3274 domain-containing protein Enterobacter kobei
9entr-a0a859r420 Klebsiella michiganensis. Transmembrane protein Klebsiella michiganensis
9entr-a0a859r420 Klebsiella michiganensis. Transmembrane protein Klebsiella michiganensis
9entr-a0a859r420 Klebsiella michiganensis. Transmembrane protein Klebsiella michiganensis
9gamm-a0a1h3wkr2 Lonsdalea quercina. DUF3274 domain-containing protein Lonsdalea quercina
9gamm-a0a1h3wkr2 Lonsdalea quercina. DUF3274 domain-containing protein Lonsdalea quercina
9gamm-a0a1h3wkr2 Lonsdalea quercina. DUF3274 domain-containing protein Lonsdalea quercina
9gamm-a0a501s7x0 Pantoea vagans. DUF3274 domain-containing protein Pantoea vagans
9gamm-a0a501s7x0 Pantoea vagans. DUF3274 domain-containing protein Pantoea vagans
9gamm-a0a501s7x0 Pantoea vagans. DUF3274 domain-containing protein Pantoea vagans
9neis-a0a0n0xja2 Amantichitinum ursilacus. DUF3274 domain-containing protein Amantichitinum ursilacus
9neis-a0a0n0xja2 Amantichitinum ursilacus. DUF3274 domain-containing protein Amantichitinum ursilacus
9neis-a0a0n0xja2 Amantichitinum ursilacus. DUF3274 domain-containing protein Amantichitinum ursilacus
9psed-a0a0q8ai33 Pseudomonas sp.Root562 , DUF3274 domain-containing protein Pseudomonas sp. Root562
9psed-a0a0q8ai33 Pseudomonas sp.Root562 , DUF3274 domain-containing protein Pseudomonas sp. Root562
9psed-a0a0q8ai33 Pseudomonas sp.Root562 , DUF3274 domain-containing protein Pseudomonas sp. Root562
9psed-a0a191yqf1 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-a0a191yqf1 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-a0a191yqf1 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-a0a1h2gh91 Pseudomonas orientalis Alpha\/beta hydrolase Pseudomonas orientalis
9psed-a0a1h2gh91 Pseudomonas orientalis Alpha\/beta hydrolase Pseudomonas orientalis
9psed-a0a1h2gh91 Pseudomonas orientalis Alpha\/beta hydrolase Pseudomonas orientalis
9psed-j2qkc5 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-j2qkc5 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-j2qkc5 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-j3btu9 Pseudomonas sp. GM74, DUF3274 domain-containing protein Pseudomonas sp. GM74
9psed-j3btu9 Pseudomonas sp. GM74, DUF3274 domain-containing protein Pseudomonas sp. GM74
9psed-j3btu9 Pseudomonas sp. GM74, DUF3274 domain-containing protein Pseudomonas sp. GM74
9psed-j3gyl1 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-j3gyl1 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9psed-j3gyl1 Pseudomonas sp., DUF3274 domain-containing protein Pseudomonas sp.
9rals-a0a160bt47 Ralstonia mannitolilytica. DUF3274 domain-containing protein Ralstonia mannitolilytica
9rals-a0a160bt47 Ralstonia mannitolilytica. DUF3274 domain-containing protein Ralstonia mannitolilytica
9rals-a0a160bt47 Ralstonia mannitolilytica. DUF3274 domain-containing protein Ralstonia mannitolilytica
azosb-a1kb81 Azoarcus sp. (strain BH72) (Azoarcus olearius) DUF3274 domain-containing protein Azoarcus olearius
azosb-a1kb81 Azoarcus sp. (strain BH72) (Azoarcus olearius) DUF3274 domain-containing protein Azoarcus olearius
azosb-a1kb81 Azoarcus sp. (strain BH72) (Azoarcus olearius) DUF3274 domain-containing protein Azoarcus olearius
burta-q2stm9 Burkholderia thailandensis (strain ATCC 700388 \/ DSM 13276 \/ CIP 106301 \/ E264). DUF3274 domain-containing protein Burkholderia thailandensis
burta-q2stm9 Burkholderia thailandensis (strain ATCC 700388 \/ DSM 13276 \/ CIP 106301 \/ E264). DUF3274 domain-containing protein Burkholderia thailandensis
burta-q2stm9 Burkholderia thailandensis (strain ATCC 700388 \/ DSM 13276 \/ CIP 106301 \/ E264). DUF3274 domain-containing protein Burkholderia thailandensis
burvg-a4jp89 Burkholderia vietnamiensis (strain G4 \/ LMG 22486) (Burkholderia cepacia (strain R1808)). DUF3274 domain-containing protein Burkholderia vietnamiensis
burvg-a4jp89 Burkholderia vietnamiensis (strain G4 \/ LMG 22486) (Burkholderia cepacia (strain R1808)). DUF3274 domain-containing protein Burkholderia vietnamiensis
burvg-a4jp89 Burkholderia vietnamiensis (strain G4 \/ LMG 22486) (Burkholderia cepacia (strain R1808)). DUF3274 domain-containing protein Burkholderia vietnamiensis
cupnh-q0kdw6 Cupriavidus necator (strain ATCC 17699 \/ DSM 428 \/ KCTC 22496 \/ NCIMB 10442 \/ H16 \/ Stanier 337) (Ralstonia eutropha). Transmembrane protein Cupriavidus necator
cupnh-q0kdw6 Cupriavidus necator (strain ATCC 17699 \/ DSM 428 \/ KCTC 22496 \/ NCIMB 10442 \/ H16 \/ Stanier 337) (Ralstonia eutropha). Transmembrane protein Cupriavidus necator
cupnh-q0kdw6 Cupriavidus necator (strain ATCC 17699 \/ DSM 428 \/ KCTC 22496 \/ NCIMB 10442 \/ H16 \/ Stanier 337) (Ralstonia eutropha). Transmembrane protein Cupriavidus necator
ecoli-T6SS.TLE3 Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435 Escherichia coli
ecoli-T6SS.TLE3 Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435 Escherichia coli
ecoli-T6SS.TLE3 Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435 Escherichia coli
erwbe-d8mxb0 Erwinia billingiae (strain Eb661). DUF3274 domain-containing protein Erwinia billingiae
erwbe-d8mxb0 Erwinia billingiae (strain Eb661). DUF3274 domain-containing protein Erwinia billingiae
erwbe-d8mxb0 Erwinia billingiae (strain Eb661). DUF3274 domain-containing protein Erwinia billingiae
paule-a0a4r3htz6 Paucimonas lemoignei (Pseudomonas lemoignei). DUF3274 domain-containing protein Paucimonas lemoignei
paule-a0a4r3htz6 Paucimonas lemoignei (Pseudomonas lemoignei). DUF3274 domain-containing protein Paucimonas lemoignei
paule-a0a4r3htz6 Paucimonas lemoignei (Pseudomonas lemoignei). DUF3274 domain-containing protein Paucimonas lemoignei
psea0-a0a0p9t0f4 Pseudomonas amygdali pv. hibisci, DUF3274 domain-containing protein Pseudomonas amygdali pv. hibisci
psea0-a0a0p9t0f4 Pseudomonas amygdali pv. hibisci, DUF3274 domain-containing protein Pseudomonas amygdali pv. hibisci
psea0-a0a0p9t0f4 Pseudomonas amygdali pv. hibisci, DUF3274 domain-containing protein Pseudomonas amygdali pv. hibisci
pseae-q9i6m9 Pseudomonas aeruginosa (strain ATCC 15692 \/ DSM 22644 \/ CIP 104116 \/ JCM 14847 \/ LMG 12228 \/ 1C \/ PRS 101 \/ PAO1). Tle3 DUF3274 domain-containing protein PA0260 Pseudomonas aeruginosa
pseae-q9i6m9 Pseudomonas aeruginosa (strain ATCC 15692 \/ DSM 22644 \/ CIP 104116 \/ JCM 14847 \/ LMG 12228 \/ 1C \/ PRS 101 \/ PAO1). Tle3 DUF3274 domain-containing protein PA0260 Pseudomonas aeruginosa
pseae-q9i6m9 Pseudomonas aeruginosa (strain ATCC 15692 \/ DSM 22644 \/ CIP 104116 \/ JCM 14847 \/ LMG 12228 \/ 1C \/ PRS 101 \/ PAO1). Tle3 DUF3274 domain-containing protein PA0260 Pseudomonas aeruginosa
psesm-q87v84 Pseudomonas syringae pv. tomato (strain ATCC BAA-871 \/ DC3000). DUF3274 domain-containing protein Pseudomonas syringae pv. tomato
psesm-q87v84 Pseudomonas syringae pv. tomato (strain ATCC BAA-871 \/ DC3000). DUF3274 domain-containing protein Pseudomonas syringae pv. tomato
psesm-q87v84 Pseudomonas syringae pv. tomato (strain ATCC BAA-871 \/ DC3000). DUF3274 domain-containing protein Pseudomonas syringae pv. tomato
psesx-a0a3m3az29 Pseudomonas syringae\; Pseudomonas amygdali pv. lachrymans str. DUF3274 domain-containing protein Pseudomonas syringae
psesx-a0a3m3az29 Pseudomonas syringae\; Pseudomonas amygdali pv. lachrymans str. DUF3274 domain-containing protein Pseudomonas syringae
psesx-a0a3m3az29 Pseudomonas syringae\; Pseudomonas amygdali pv. lachrymans str. DUF3274 domain-containing protein Pseudomonas syringae
psesx-w0mzj5 Pseudomonas syringae, DUF3274 domain-containing protein Pseudomonas syringae
psesx-w0mzj5 Pseudomonas syringae, DUF3274 domain-containing protein Pseudomonas syringae
psesx-w0mzj5 Pseudomonas syringae, DUF3274 domain-containing protein Pseudomonas syringae
ralsl-a3rp80 Ralstonia solanacearum UW551 Putative uncharacterized protein Ralstonia solanacearum UW551
ralsl-a3rp80 Ralstonia solanacearum UW551 Putative uncharacterized protein Ralstonia solanacearum UW551
ralsl-a3rp80 Ralstonia solanacearum UW551 Putative uncharacterized protein Ralstonia solanacearum UW551
ralso-q8y0t3 Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum). DUF3274 domain-containing protein Ralstonia solanacearum
ralso-q8y0t3 Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum). DUF3274 domain-containing protein Ralstonia solanacearum
ralso-q8y0t3 Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum). DUF3274 domain-containing protein Ralstonia solanacearum
serfi-a0a240bv21 Serratia ficaria. DUF3274 domain-containing protein Serratia ficaria
serfi-a0a240bv21 Serratia ficaria. DUF3274 domain-containing protein Serratia ficaria
serfi-a0a240bv21 Serratia ficaria. DUF3274 domain-containing protein Serratia ficaria
xanop-a0a0k0gpc4 Xanthomonas oryzae pv. oryzae (strain PXO99A). DUF3274 domain-containing protein Xanthomonas oryzae pv. oryzae
xanop-a0a0k0gpc4 Xanthomonas oryzae pv. oryzae (strain PXO99A). DUF3274 domain-containing protein Xanthomonas oryzae pv. oryzae
xanop-a0a0k0gpc4 Xanthomonas oryzae pv. oryzae (strain PXO99A). DUF3274 domain-containing protein Xanthomonas oryzae pv. oryzae

Gene_locus_frgt Name Species
9psed-i4mv51 Pseudomonas sp., Uncharacterized protein Pseudomonas sp.
9psed-i4mv51 Pseudomonas sp., Uncharacterized protein Pseudomonas sp.
9psed-i4mv51 Pseudomonas sp., Uncharacterized protein Pseudomonas sp.

Substrate Chemical Nomenclature Proteins
PED-A1 N-{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza-s-indacene-3-pentanoyl}-2-hexyl-sn-glycero-3-phosphoethanolamine human-LIPH    ecoli-T6SS.TLE3
PED-A1 N-{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza-s-indacene-3-pentanoyl}-2-hexyl-sn-glycero-3-phosphoethanolamine human-LIPH    ecoli-T6SS.TLE3
PED-A1 N-{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza-s-indacene-3-pentanoyl}-2-hexyl-sn-glycero-3-phosphoethanolamine human-LIPH    ecoli-T6SS.TLE3

References (6)

Title : Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS Effector\/Immunity Complex Determined Using an AlphaFold2 Predicted Model - Le_2023_Int.J.Mol.Sci_24_1740
Author(s) : Le TTH , Kellenberger C , Boyer M , Santucci P , Flaugnatti N , Cascales E , Roussel A , Canaan S , Journet L , Cambillau C
Ref : Int J Mol Sci , 24 :1740 , 2023
Abstract :
PubMedSearch : Le_2023_Int.J.Mol.Sci_24_1740
PubMedID: 36675258
Gene_locus related to this paper: ecoli-T6SS.TLE3

Title : Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS Effector\/Immunity Complex Determined Using an AlphaFold2 Predicted Model - Le_2023_Int.J.Mol.Sci_24_1740
Author(s) : Le TTH , Kellenberger C , Boyer M , Santucci P , Flaugnatti N , Cascales E , Roussel A , Canaan S , Journet L , Cambillau C
Ref : Int J Mol Sci , 24 :1740 , 2023
Abstract :
PubMedSearch : Le_2023_Int.J.Mol.Sci_24_1740
PubMedID: 36675258
Gene_locus related to this paper: ecoli-T6SS.TLE3

Title : Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS Effector\/Immunity Complex Determined Using an AlphaFold2 Predicted Model - Le_2023_Int.J.Mol.Sci_24_1740
Author(s) : Le TTH , Kellenberger C , Boyer M , Santucci P , Flaugnatti N , Cascales E , Roussel A , Canaan S , Journet L , Cambillau C
Ref : Int J Mol Sci , 24 :1740 , 2023
Abstract :
PubMedSearch : Le_2023_Int.J.Mol.Sci_24_1740
PubMedID: 36675258
Gene_locus related to this paper: ecoli-T6SS.TLE3

Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508
Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD
Ref : Nature , 496 :508 , 2013
Abstract :
PubMedSearch : Russell_2013_Nature_496_508
PubMedID: 23552891
Gene_locus related to this paper: ecoli-T6SS.TLE3

Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508
Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD
Ref : Nature , 496 :508 , 2013
Abstract :
PubMedSearch : Russell_2013_Nature_496_508
PubMedID: 23552891
Gene_locus related to this paper: ecoli-T6SS.TLE3

Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508
Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD
Ref : Nature , 496 :508 , 2013
Abstract :
PubMedSearch : Russell_2013_Nature_496_508
PubMedID: 23552891
Gene_locus related to this paper: ecoli-T6SS.TLE3