T6SS-TLE3
Comment
Family of Tle3 phospholipase effector 
.The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 family (now T6SS-TLE1), TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase (now T6SS-TLE3) and Tle4 belongs to PGAP1 (Tle5 family, such as Pseudomonas aeruginosa PldA and PldB, are phospholipases D and are not alpha/beta hydrolases). Tle3 phospholipase effector from adherent-invasive Escherichia coli (AIEC) Tle3AIEC possesses phospholipase A1 (PLA1) activity (specific activity (SA) = 7.65 nmole.min-1.mg-1) but no PLA2 activity. The T6SS-TLE3 is decorated by two protruding segments, including a N-terminus loop containing part of the oxyanion hole residues. The long C-terminus contains the catalytic site histidine and in some members is related to the DUF3274 family (most members of this family are not linked to alpha/beta hydrolases). In T6SS, protection against its own toxic effector is ensured in bacteria by the production of immunity proteins that specifically bind and inhibit Tle3. Tli (Type VI lipase immunity) binds tightly to Tle3. There is also a strong interaction between the VgrGAIEC C-terminus adaptor and Tle3AIEC N-terminal loop.
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 8BOZ | Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS effector\/immunity complex | ecoli-T6SS.TLE3 |
| Gene_locus | Name | Species |
|---|---|---|
| ecoli-T6SS.TLE3 |  Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435 |
Escherichia coli |
| ralsl-a3rp80 | Ralstonia solanacearum UW551 Putative uncharacterized protein | Ralstonia solanacearum UW551 |
| erwbe-d8mxb0 | Erwinia billingiae (strain Eb661). DUF3274 domain-containing protein | Erwinia billingiae |
| 9burk-a0a069pb40 | Caballeronia glathei. DUF3274 domain-containing protein | Caballeronia glathei |
| 9entr-a0a089px10 | Cedecea neteri. DUF3274 domain-containing protein | Cedecea neteri |
| xanop-a0a0k0gpc4 | Xanthomonas oryzae pv. oryzae (strain PXO99A). DUF3274 domain-containing protein | Xanthomonas oryzae pv. oryzae |
| 9neis-a0a0n0xja2 | Amantichitinum ursilacus. DUF3274 domain-containing protein | Amantichitinum ursilacus |
| 9burk-a0a0q5nyl5 | Burkholderia sp. Leaf177. DUF3274 domain-containing protein | Burkholderia sp. Leaf177 |
| 9burk-a0a126zda3 | Variovorax sp. PAMC 28711. Transmembrane protein | Variovorax sp. PAMC 28711 |
| 9rals-a0a160bt47 | Ralstonia mannitolilytica. DUF3274 domain-containing protein | Ralstonia mannitolilytica |
| 9gamm-a0a1h3wkr2 | Lonsdalea quercina. DUF3274 domain-containing protein | Lonsdalea quercina |
| 9burk-a0a1i4t633 | Rugamonas rubra. DUF3274 domain-containing protein | Rugamonas rubra |
| 9burk-a0a1i4u0b9 | Rugamonas rubra. DUF3274 domain-containing protein | Rugamonas rubra |
| 9burk-a0a1p8jq21 | Rhodoferax koreense. DUF3274 domain-containing protein | Rhodoferax koreense |
| 9burk-a0a1q8iqv1 | Burkholderia sp. SRS-W-2-2016. DUF3274 domain-containing protein | Burkholderia sp. SRS-W-2-2016 |
| serfi-a0a240bv21 | Serratia ficaria. DUF3274 domain-containing protein | Serratia ficaria |
| 9entr-a0a2j0pr84 | Enterobacter kobei. DUF3274 domain-containing protein | Enterobacter kobei |
| 9burk-a0a2r4c9j8 | Pseudoduganella armeniaca. Transmembrane protein | Pseudoduganella armeniaca |
| 9burk-a0a3d8k099 | Trinickia dinghuensis. Transmembrane protein | Trinickia dinghuensis |
| paule-a0a4r3htz6 | Paucimonas lemoignei (Pseudomonas lemoignei). DUF3274 domain-containing protein | Paucimonas lemoignei |
| 9gamm-a0a501s7x0 | Pantoea vagans. DUF3274 domain-containing protein | Pantoea vagans |
| 9burk-a0a7z2zsr3 | Massilia forsythiae. Transmembrane protein | Massilia forsythiae |
| 9entr-a0a859r420 | Klebsiella michiganensis. Transmembrane protein | Klebsiella michiganensis |
| azosb-a1kb81 | Azoarcus sp. (strain BH72) (Azoarcus olearius) DUF3274 domain-containing protein | Azoarcus olearius |
| burvg-a4jp89 | Burkholderia vietnamiensis (strain G4 \/ LMG 22486) (Burkholderia cepacia (strain R1808)). DUF3274 domain-containing protein | Burkholderia vietnamiensis |
| cupnh-q0kdw6 | Cupriavidus necator (strain ATCC 17699 \/ DSM 428 \/ KCTC 22496 \/ NCIMB 10442 \/ H16 \/ Stanier 337) (Ralstonia eutropha). Transmembrane protein | Cupriavidus necator |
| burta-q2stm9 | Burkholderia thailandensis (strain ATCC 700388 \/ DSM 13276 \/ CIP 106301 \/ E264). DUF3274 domain-containing protein | Burkholderia thailandensis |
| psesm-q87v84 | Pseudomonas syringae pv. tomato (strain ATCC BAA-871 \/ DC3000). DUF3274 domain-containing protein | Pseudomonas syringae pv. tomato |
| ralso-q8y0t3 | Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum). DUF3274 domain-containing protein | Ralstonia solanacearum |
| pseae-q9i6m9 | Pseudomonas aeruginosa (strain ATCC 15692 \/ DSM 22644 \/ CIP 104116 \/ JCM 14847 \/ LMG 12228 \/ 1C \/ PRS 101 \/ PAO1). Tle3 DUF3274 domain-containing protein PA0260 | Pseudomonas aeruginosa |
| 9burk-w0vf87 | Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628. DUF3274 domain-containing protein | Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628 |
| psesx-a0a3m3az29 | Pseudomonas syringae\; Pseudomonas amygdali pv. lachrymans str. DUF3274 domain-containing protein | Pseudomonas syringae |
| 9psed-a0a1h2gh91 | Pseudomonas orientalis Alpha\/beta hydrolase | Pseudomonas orientalis |
| 9psed-j2qkc5 | Pseudomonas sp., DUF3274 domain-containing protein | Pseudomonas sp. |
| 9psed-a0a191yqf1 | Pseudomonas sp., DUF3274 domain-containing protein | Pseudomonas sp. |
| 9psed-a0a0q8ai33 | Pseudomonas sp.Root562 , DUF3274 domain-containing protein | Pseudomonas sp. Root562 |
| psea0-a0a0p9t0f4 | Pseudomonas amygdali pv. hibisci, DUF3274 domain-containing protein | Pseudomonas amygdali pv. hibisci |
| psesx-w0mzj5 | Pseudomonas syringae, DUF3274 domain-containing protein | Pseudomonas syringae |
| 9psed-j3gyl1 | Pseudomonas sp., DUF3274 domain-containing protein | Pseudomonas sp. |
| 9psed-j3btu9 | Pseudomonas sp. GM74, DUF3274 domain-containing protein | Pseudomonas sp. GM74 |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9psed-i4mv51 | Pseudomonas sp., Uncharacterized protein | Pseudomonas sp. |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| PED-A1 | N-{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza-s-indacene-3-pentanoyl}-2-hexyl-sn-glycero-3-phosphoethanolamine | human-LIPH ecoli-T6SS.TLE3 |
References (2)
| Title : Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3\/Tli3 T6SS Effector\/Immunity Complex Determined Using an AlphaFold2 Predicted Model - Le_2023_Int.J.Mol.Sci_24_1740 |
| Author(s) : Le TTH , Kellenberger C , Boyer M , Santucci P , Flaugnatti N , Cascales E , Roussel A , Canaan S , Journet L , Cambillau C |
| Ref : Int J Mol Sci , 24 :1740 , 2023 |
| Abstract : |
| PubMedSearch : Le_2023_Int.J.Mol.Sci_24_1740 |
| PubMedID: 36675258 |
| Gene_locus related to this paper: ecoli-T6SS.TLE3 |
| Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508 |
| Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD |
| Ref : Nature , 496 :508 , 2013 |
| Abstract : |
| PubMedSearch : Russell_2013_Nature_496_508 |
| PubMedID: 23552891 |
| Gene_locus related to this paper: ecoli-T6SS.TLE3 |