(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Tunicata: NE > Ascidiacea: NE > Enterogona: NE > Phlebobranchia: NE > Cionidae: NE > Ciona: NE > Ciona savignyi: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MFVFIFAIFLLNDITVVSTSPSRNQFQRIFRSPDWTNIMKRDAYSRIQNQ QSNDFSEEVLDQRIEAIKFVQIRTNSGRVRGHKTVLENQSIKPVVKFLGI PYAAPPVGKFRWKKTEKPKPWDGVRNASTFGPICPQARNGPLPAALLPVW YRANHSLVQRMRMDEDCLYLNIYVPAKLYASGKLDLILPSLCLTLICRNL CNPLPVMVHFHGYTYAEGSGNFYDGSVLASYGEVIVVTFNYRLGVLGFMS TMESNSPGNYGLWDQVAALKWVSENIDRFGGDPNSVTVFGSGAGASCIGL LMVSVQLDAYFQRAILQSGTALAPWSMVRNPRQQTLGLARAPSVNCYRES SREMVECLKGKSWRDLISVAISTEPYDLAFSPVVDGQDMFLVDNPFNLME KGEFLNYPVMIGVSPGDGFGYLRDRILYPSGKSFGSDLFDVVVAKFTDSF YDDSEVPLEAIEKLVRFIYTDWADRENPMRLKSSLMELYTDRQFVEPAVR TALHNTNYKNNAFFYTFYHHPGKDPNRSWIESALGEQDPFVFGAPLMGNS AETLFPYNYTKDDIMISTAVMTYWTNFAKNGDPGKGKKQQTRFITEKANC FENVVWPQYEEAGRQHLKISTSPEVGSHYRAQKVELWRSFLPSLSGVSRS SIGDGVNPLKPPRAEPPTISPGTKNPNLATPKVSIFYSDTLTNHRSQPKP STSETNGLSLQLNITLAVGFVLLFLNIIAFAVVSYHKEKDKYKIDVSTVK T
Neuroligins constitute a family of transmembrane proteins localized at the postsynaptic side of both excitatory and inhibitory synapses of the central nervous system. They are involved in synaptic function and maturation and recent studies have linked mutations in specific human Neuroligins to mental retardation and autism. We isolated the human Neuroligin homologs in Danio rerio. Next, we studied their gene structures and we reconstructed the evolution of the Neuroligin genes across vertebrate phyla. Using reverse-transcriptase polymerase chain reaction, we analyzed the expression and alternative splicing pattern of each gene during zebrafish embryonic development and in different adult organs. By in situ hybridization, we analyzed the temporal and spatial expression pattern during embryonic development and larval stages and we found that zebrafish Neuroligins are expressed throughout the nervous system. Globally, our results indicate that, during evolution, specific subfunctionalization events occurred within paralogous members of this gene family in zebrafish.
