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Gene_locus Report for: ecoli-T6SS.TLE3

Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435

Comment
Family of Tle3 phospholipase effector .The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 family, TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase and Tle4 belongs to PGAP1 (Tle5 family, such as Pseudomonas aeruginosa PldA and PldB, are phospholipases D and are not alpha/beta hydrolases). Tle3 phospholipase effector from adherent-invasive Escherichia coli (AIEC) Tle3AIEC possesses phospholipase A1 (PLA1) activity (specific activity (SA) = 7.65 nmole.min-1.mg-1) but no PLA2 activity. . The T6SS-TLE3 is decorated by two protruding segments, including a N-terminus loop containing part of the oxyanion hole residues. The long C-terminus contains the catalytic site histidine and in some members is related to the DUF3274 family (most members of this family are not linked to alpha/beta hydrolases).In T6SS, protection against its own toxic effector is ensured in bacteria by the production of immunity proteins that specifically bind and inhibit Tle3. Tli (Type VI lipase immunity) binds tightly to Tle3. There is also a strong interaction between the VgrGAIEC C-terminus adaptor and Tle3AIEC N-terminal loop. Other strains: Escherichia coli O83:H1 (strain NRG 857C / AIEC); Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)


Relationship
Family|T6SS-TLE3
Block| X
Position in NCBI Life Tree|Escherichia coli
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Proteobacteria: N E > Gammaproteobacteria: N E > Enterobacterales: N E > Enterobacteriaceae: N E > Escherichia: N E > Escherichia coli: N E
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data


Molecular evidence
Database
No mutation
1 structure:
8BOZ: Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS effector/immunity complex
No kinetic





1 substrate:
PED-A1
No inhibitor
Sequence
Graphical view for this peptide sequence: ecoli-T6SS.TLE3
Colored MSA for T6SS-TLE3 (raw)
MSTNKSEPTRKVDVHLTDNGTPFAYSMTSHKNVKVRAEVQPPLQLPGLII
FVHGVNSEGEWYDYAERSLCAGLNQRLGLEGEHGLKENNYEGGFFVNSDK
SEGGWEHTYEIEGSQKKWVSGPRKITKGGDGRSPVIRFYWGYRAADNETD
TYAIPLKNKKGDNYYDLPPESRKAKGPWFWGGGPFQNGCNQLVSLWSKTG
FNNNPSLLGVPLPFSTQVLNGERDRLLSDAPPRHYYAHAAGRLAKLIKTI
RNQHPEDTVTVLSHSQGTMIALAAAAIEAPDALFVMNSPYALENEPTTYI
SYPIKEIISRKARSATFADIVKKVAENKTRLKQQGCDNLLAGMSSDGNSW
IPEGKTHNGLPERDNHGTTWIYCNPHDRVMGSSPLRSIGWQGLPDTKDGT
PHTLFKQAGDTLYVRILGRNTPCGGTPTAQTHFSNLGDGKPFWDSTTTLL
QRATWPDPDSGQTLTINAPQVPEPLTAEELKNFDQDYARDEKQSGGAGYA
YGQINPETKKPVDTDYRYYISLYGYFDRKMVPKKDSGYYQSGPGSKEDRV
KYEKQSQEEMLEEVRTYVQRPTDHSTLPSDERFMSRVVAYDLPIGYCWHS
WDKAGLEELRRQADWLESDDYYFSGKLTVPPIPPAIKQDVAEDAEQRKAE
EKARLRNV
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MSTNKSEPTRKVDVHLTDNGTPFAYSMTSHKNVKVRAEVQPPLQLPGLII
FVHGVNSEGEWYDYAERSLCAGLNQRLGLEGEHGLKENNYEGGFFVNSDK
SEGGWEHTYEIEGSQKKWVSGPRKITKGGDGRSPVIRFYWGYRAADNETD
TYAIPLKNKKGDNYYDLPPESRKAKGPWFWGGGPFQNGCNQLVSLWSKTG
FNNNPSLLGVPLPFSTQVLNGERDRLLSDAPPRHYYAHAAGRLAKLIKTI
RNQHPEDTVTVLSHSQGTMIALAAAAIEAPDALFVMNSPYALENEPTTYI
SYPIKEIISRKARSATFADIVKKVAENKTRLKQQGCDNLLAGMSSDGNSW
IPEGKTHNGLPERDNHGTTWIYCNPHDRVMGSSPLRSIGWQGLPDTKDGT
PHTLFKQAGDTLYVRILGRNTPCGGTPTAQTHFSNLGDGKPFWDSTTTLL
QRATWPDPDSGQTLTINAPQVPEPLTAEELKNFDQDYARDEKQSGGAGYA
YGQINPETKKPVDTDYRYYISLYGYFDRKMVPKKDSGYYQSGPGSKEDRV
KYEKQSQEEMLEEVRTYVQRPTDHSTLPSDERFMSRVVAYDLPIGYCWHS
WDKAGLEELRRQADWLESDDYYFSGKLTVPPIPPAIKQDVAEDAEQRKAE
EKARLRNV


References
    Title: Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS Effector/Immunity Complex Determined Using an AlphaFold2 Predicted Model
    Le TTH, Kellenberger C, Boyer M, Santucci P, Flaugnatti N, Cascales E, Roussel A, Canaan S, Journet L, Cambillau C
    Ref: Int J Mol Sci, 24:1740, 2023 : PubMed

            

    Title: Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors
    Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
    Ref: Nature, 496:508, 2013 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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