(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Fusobacteria: NE > Fusobacteriia: NE > Fusobacteriales: NE > Fusobacteriaceae: NE > Fusobacterium: NE > Fusobacterium nucleatum: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Fusobacterium nucleatum subsp. nucleatum ATCC 25586: N, E.
Fusobacterium nucleatum subsp. nucleatum: N, E.
Fusobacterium nucleatum subsp. nucleatum ATCC 23726: N, E.
Fusobacterium nucleatum subsp. polymorphum F0401: N, E.
Fusobacterium nucleatum subsp. animalis ATCC 51191: N, E.
Molecular evidence
Database
No mutation 1 structure: 6YQ4: Crystal structure of Fusobacterium nucleatum tannase No kinetic
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MVKNDYDLKFNPDKYISKEIKINGKKIKYRAYENIIYIKNPIDKDYQNMN IYIPEEYFNNLSIGSYNSNNAPIFFPNTVGGYMPGKADTVGLGRDGKANS LTYALSKGYVVAAPGARGRTLTDDKGNYIGKAPAAIVDLKAAVRYLYLND EVMPGDANKIISNGTSAGGALSALLGASGNSQDYLPYLKEIGAAETRDDI FAVSAYCPITNLENADSAYEWMYNGVNSYSRMEFTRNTSAQEYNDRSLTR STVQGNLTNDEINISNKLKTLFPIYLNSLKLTDDGGNLLTLDKSGNGSFK TYLSIIIRNSANRALREGKDISQFKKAFTIENNKVVAVNLDVYTHIGDRM KSPPAFDSLDASSGENNLFGDKKSDSKHFTKFSFDINNKAAIDYFRNGKF NDKNNKISIPKMADKNIIKMMNPMYYIDSNTSTKYWRIRHGAIDKDTSLA IPAILALKLKNSGKIVNFAAPWGQGHGGDYDLEELFNWIDNVVKK
Colorectal cancer pathogenesis and progression is associated with the presence of Fusobacterium nucleatum and the reduction of acetylated derivatives of spermidine, as well as dietary components such as tannin-rich foods. We show that a new tannase orthologue of F. nucleatum (TanBF(nn) ) has significant structural differences with its Lactobacillus plantarum counterpart affecting the flap covering the active site and the accessibility of substrates. Crystallographic and molecular dynamics analysis revealed binding of polyamines to a small cavity that connects the active site with the bulk solvent which interact with catalytically indispensable residues. As a result, spermidine and its derivatives, particularly N(8) -acetylated spermidine, inhibit the hydrolytic activity of TanBF(nn) and increase the toxicity of gallotannins to F. nucleatum. Our results support a model in which the balance between the detoxicant activity of TanBF(nn) and the presence of metabolic inhibitors can dictate either conducive or unfavourable conditions for the survival of F. nucleatum.
We present a complete DNA sequence and metabolic analysis of the dominant oral bacterium Fusobacterium nucleatum. Although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens Porphyromonas gingivalis and Bacteroides forsythus. The F. nucleatum strain ATCC 25586 genome was assembled from shotgun sequences and analyzed using the ERGO bioinformatics suite (http://www.integratedgenomics.com). The genome contains 2.17 Mb encoding 2,067 open reading frames, organized on a single circular chromosome with 27% GC content. Despite its taxonomic position among the gram-negative bacteria, several features of its core metabolism are similar to that of gram-positive Clostridium spp., Enterococcus spp., and Lactococcus spp. The genome analysis has revealed several key aspects of the pathways of organic acid, amino acid, carbohydrate, and lipid metabolism. Nine very-high-molecular-weight outer membrane proteins are predicted from the sequence, none of which has been reported in the literature. More than 137 transporters for the uptake of a variety of substrates such as peptides, sugars, metal ions, and cofactors have been identified. Biosynthetic pathways exist for only three amino acids: glutamate, aspartate, and asparagine. The remaining amino acids are imported as such or as di- or oligopeptides that are subsequently degraded in the cytoplasm. A principal source of energy appears to be the fermentation of glutamate to butyrate. Additionally, desulfuration of cysteine and methionine yields ammonia, H(2)S, methyl mercaptan, and butyrate, which are capable of arresting fibroblast growth, thus preventing wound healing and aiding penetration of the gingival epithelium. The metabolic capabilities of F. nucleatum revealed by its genome are therefore consistent with its specialized niche in the mouth.
Fusobacterium nucleatum hypothetical protein fn0616 Alpha/beta superfamily hydrolase
