Goltsman E

References (15)

Title : Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an abundant inhabitant of rice paddy soil ecosystems - van Passel_2011_J.Bacteriol_193_2367
Author(s) : van Passel MW , Kant R , Palva A , Copeland A , Lucas S , Lapidus A , Glavina Del Rio T , Pitluck S , Goltsman E , Clum A , Sun H , Schmutz J , Larimer FW , Land ML , Hauser L , Kyrpides N , Mikhailova N , Richardson PP , Janssen PH , de Vos WM , Smidt H
Ref : Journal of Bacteriology , 193 :2367 , 2011
Abstract : Bacteria of the deeply branching phylum Verrucomicrobia are rarely cultured yet commonly detected in metagenomic libraries from aquatic, terrestrial, and intestinal environments. We have sequenced the genome of Opitutus terrae PB90-1, a fermentative anaerobe within this phylum, isolated from rice paddy soil and capable of propionate production from plant-derived polysaccharides.
ESTHER : van Passel_2011_J.Bacteriol_193_2367
PubMedSearch : van Passel_2011_J.Bacteriol_193_2367
PubMedID: 21398538
Gene_locus related to this paper: opitp-b1zrm2

Title : The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri - Frese_2011_PLoS.Genet_7_e1001314
Author(s) : Frese SA , Benson AK , Tannock GW , Loach DM , Kim J , Zhang M , Oh PL , Heng NC , Patil PB , Juge N , Mackenzie DA , Pearson BM , Lapidus A , Dalin E , Tice H , Goltsman E , Land M , Hauser L , Ivanova N , Kyrpides NC , Walter J
Ref : PLoS Genet , 7 :e1001314 , 2011
Abstract : Recent research has provided mechanistic insight into the important contributions of the gut microbiota to vertebrate biology, but questions remain about the evolutionary processes that have shaped this symbiosis. In the present study, we showed in experiments with gnotobiotic mice that the evolution of Lactobacillus reuteri with rodents resulted in the emergence of host specialization. To identify genomic events marking adaptations to the murine host, we compared the genome of the rodent isolate L. reuteri 100-23 with that of the human isolate L. reuteri F275, and we identified hundreds of genes that were specific to each strain. In order to differentiate true host-specific genome content from strain-level differences, comparative genome hybridizations were performed to query 57 L. reuteri strains originating from six different vertebrate hosts in combination with genome sequence comparisons of nine strains encompassing five phylogenetic lineages of the species. This approach revealed that rodent strains, although showing a high degree of genomic plasticity, possessed a specific genome inventory that was rare or absent in strains from other vertebrate hosts. The distinct genome content of L. reuteri lineages reflected the niche characteristics in the gastrointestinal tracts of their respective hosts, and inactivation of seven out of eight representative rodent-specific genes in L. reuteri 100-23 resulted in impaired ecological performance in the gut of mice. The comparative genomic analyses suggested fundamentally different trends of genome evolution in rodent and human L. reuteri populations, with the former possessing a large and adaptable pan-genome while the latter being subjected to a process of reductive evolution. In conclusion, this study provided experimental evidence and a molecular basis for the evolution of host specificity in a vertebrate gut symbiont, and it identified genomic events that have shaped this process.
ESTHER : Frese_2011_PLoS.Genet_7_e1001314
PubMedSearch : Frese_2011_PLoS.Genet_7_e1001314
PubMedID: 21379339
Gene_locus related to this paper: lacre-b3xl60 , lacrj-b2g622 , lacre-a0a0s4nmr3

Title : Complete genome sequence of Meiothermus ruber type strain (21) - Tindall_2010_Stand.Genomic.Sci_3_26
Author(s) : Tindall BJ , Sikorski J , Lucas S , Goltsman E , Copeland A , Glavina Del Rio T , Nolan M , Tice H , Cheng JF , Han C , Pitluck S , Liolios K , Ivanova N , Mavromatis K , Ovchinnikova G , Pati A , Fahnrich R , Goodwin L , Chen A , Palaniappan K , Land M , Hauser L , Chang YJ , Jeffries CD , Rohde M , Goker M , Woyke T , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP , Lapidus A
Ref : Stand Genomic Sci , 3 :26 , 2010
Abstract : Meiothermus ruber (Loginova et al. 1984) Nobre et al. 1996 is the type species of the genus Meiothermus. This thermophilic genus is of special interest, as its members share relatively low degrees of 16S rRNA gene sequence similarity and constitute a separate evolutionary lineage from members of the genus Thermus, from which they can generally be distinguished by their slightly lower temperature optima. The temperature related split is in accordance with the chemotaxonomic feature of the polar lipids. M. ruber is a representative of the low-temperature group. This is the first completed genome sequence of the genus Meiothermus and only the third genome sequence to be published from a member of the family Thermaceae. The 3,097,457 bp long genome with its 3,052 protein-coding and 53 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Tindall_2010_Stand.Genomic.Sci_3_26
PubMedSearch : Tindall_2010_Stand.Genomic.Sci_3_26
PubMedID: 21304689
Gene_locus related to this paper: meird-d3pkm5 , meird-d3pnp5 , meird-d3pnr1 , meird-d3pnw2 , meird-d3pq15 , meird-d3pqm5 , meird-d3ps60

Title : Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis - McBride_2009_Appl.Environ.Microbiol_75_6864
Author(s) : McBride MJ , Xie G , Martens EC , Lapidus A , Henrissat B , Rhodes RG , Goltsman E , Wang W , Xu J , Hunnicutt DW , Staroscik AM , Hoover TR , Cheng YQ , Stein JL
Ref : Applied Environmental Microbiology , 75 :6864 , 2009
Abstract : The 6.10-Mb genome sequence of the aerobic chitin-digesting gliding bacterium Flavobacterium johnsoniae (phylum Bacteroidetes) is presented. F. johnsoniae is a model organism for studies of bacteroidete gliding motility, gene regulation, and biochemistry. The mechanism of F. johnsoniae gliding is novel, and genome analysis confirms that it does not involve well-studied motility organelles, such as flagella or type IV pili. The motility machinery is composed of Gld proteins in the cell envelope that are thought to comprise the "motor" and SprB, which is thought to function as a cell surface adhesin that is propelled by the motor. Analysis of the genome identified genes related to sprB that may encode alternative adhesins used for movement over different surfaces. Comparative genome analysis revealed that some of the gld and spr genes are found in nongliding bacteroidetes and may encode components of a novel protein secretion system. F. johnsoniae digests proteins, and 125 predicted peptidases were identified. F. johnsoniae also digests numerous polysaccharides, and 138 glycoside hydrolases, 9 polysaccharide lyases, and 17 carbohydrate esterases were predicted. The unexpected ability of F. johnsoniae to digest hemicelluloses, such as xylans, mannans, and xyloglucans, was predicted based on the genome analysis and confirmed experimentally. Numerous predicted cell surface proteins related to Bacteroides thetaiotaomicron SusC and SusD, which are likely involved in binding of oligosaccharides and transport across the outer membrane, were also identified. Genes required for synthesis of the novel outer membrane flexirubin pigments were identified by a combination of genome analysis and genetic experiments. Genes predicted to encode components of a multienzyme nonribosomal peptide synthetase were identified, as were novel aspects of gene regulation. The availability of techniques for genetic manipulation allows rapid exploration of the features identified for the polysaccharide-digesting gliding bacteroidete F. johnsoniae.
ESTHER : McBride_2009_Appl.Environ.Microbiol_75_6864
PubMedSearch : McBride_2009_Appl.Environ.Microbiol_75_6864
PubMedID: 19717629
Gene_locus related to this paper: flaj1-a5fb12 , flaj1-a5fmd7 , flaj1-a5fns3

Title : Complete genome sequence of Methanocorpusculum labreanum type strain Z - Anderson_2009_Stand.Genomic.Sci_1_197
Author(s) : Anderson IJ , Sieprawska-Lupa M , Goltsman E , Lapidus A , Copeland A , Glavina Del Rio T , Tice H , Dalin E , Barry K , Pitluck S , Hauser L , Land M , Lucas S , Richardson P , Whitman WB , Kyrpides NC
Ref : Stand Genomic Sci , 1 :197 , 2009
Abstract : Methanocorpusculum labreanum is a methanogen belonging to the order Methanomicrobiales within the archaeal kingdom Euryarchaeota. The type strain Z was isolated from surface sediments of Tar Pit Lake in the La Brea Tar Pits in Los Angeles, California. M. labreanum is of phylogenetic interest because at the time the sequencing project began only one genome had previously been sequenced from the order Methanomicrobiales. We report here the complete genome sequence of M. labreanum type strain Z and its annotation. This is part of a 2006 Joint Genome Institute Community Sequencing Program project to sequence genomes of diverse Archaea.
ESTHER : Anderson_2009_Stand.Genomic.Sci_1_197
PubMedSearch : Anderson_2009_Stand.Genomic.Sci_1_197
PubMedID: 21304657
Gene_locus related to this paper: metlz-a2sqw3 , metlz-a2su60 , metlz-a2su61 , metlz-a2su62

Title : Localized plasticity in the streamlined genomes of vinyl chloride respiring Dehalococcoides - McMurdie_2009_PLoS.Genet_5_e1000714
Author(s) : McMurdie PJ , Behrens SF , Muller JA , Goke J , Ritalahti KM , Wagner R , Goltsman E , Lapidus A , Holmes S , Loffler FE , Spormann AM
Ref : PLoS Genet , 5 :e1000714 , 2009
Abstract : Vinyl chloride (VC) is a human carcinogen and widespread priority pollutant. Here we report the first, to our knowledge, complete genome sequences of microorganisms able to respire VC, Dehalococcoides sp. strains VS and BAV1. Notably, the respective VC reductase encoding genes, vcrAB and bvcAB, were found embedded in distinct genomic islands (GEIs) with different predicted integration sites, suggesting that these genes were acquired horizontally and independently by distinct mechanisms. A comparative analysis that included two previously sequenced Dehalococcoides genomes revealed a contextually conserved core that is interrupted by two high plasticity regions (HPRs) near the Ori. These HPRs contain the majority of GEIs and strain-specific genes identified in the four Dehalococcoides genomes, an elevated number of repeated elements including insertion sequences (IS), as well as 91 of 96 rdhAB, genes that putatively encode terminal reductases in organohalide respiration. Only three core rdhA orthologous groups were identified, and only one of these groups is supported by synteny. The low number of core rdhAB, contrasted with the high rdhAB numbers per genome (up to 36 in strain VS), as well as their colocalization with GEIs and other signatures for horizontal transfer, suggests that niche adaptation via organohalide respiration is a fundamental ecological strategy in Dehalococccoides. This adaptation has been exacted through multiple mechanisms of recombination that are mainly confined within HPRs of an otherwise remarkably stable, syntenic, streamlined genome among the smallest of any free-living microorganism.
ESTHER : McMurdie_2009_PLoS.Genet_5_e1000714
PubMedSearch : McMurdie_2009_PLoS.Genet_5_e1000714
PubMedID: 19893622
Gene_locus related to this paper: dehm1-q3z8f3 , dehmv-d2bg80

Title : A korarchaeal genome reveals insights into the evolution of the Archaea - Elkins_2008_Proc.Natl.Acad.Sci.U.S.A_105_8102
Author(s) : Elkins JG , Podar M , Graham DE , Makarova KS , Wolf Y , Randau L , Hedlund BP , Brochier-Armanet C , Kunin V , Anderson I , Lapidus A , Goltsman E , Barry K , Koonin EV , Hugenholtz P , Kyrpides N , Wanner G , Richardson P , Keller M , Stetter KO
Ref : Proc Natl Acad Sci U S A , 105 :8102 , 2008
Abstract : The candidate division Korarchaeota comprises a group of uncultivated microorganisms that, by their small subunit rRNA phylogeny, may have diverged early from the major archaeal phyla Crenarchaeota and Euryarchaeota. Here, we report the initial characterization of a member of the Korarchaeota with the proposed name, "Candidatus Korarchaeum cryptofilum," which exhibits an ultrathin filamentous morphology. To investigate possible ancestral relationships between deep-branching Korarchaeota and other phyla, we used whole-genome shotgun sequencing to construct a complete composite korarchaeal genome from enriched cells. The genome was assembled into a single contig 1.59 Mb in length with a G + C content of 49%. Of the 1,617 predicted protein-coding genes, 1,382 (85%) could be assigned to a revised set of archaeal Clusters of Orthologous Groups (COGs). The predicted gene functions suggest that the organism relies on a simple mode of peptide fermentation for carbon and energy and lacks the ability to synthesize de novo purines, CoA, and several other cofactors. Phylogenetic analyses based on conserved single genes and concatenated protein sequences positioned the korarchaeote as a deep archaeal lineage with an apparent affinity to the Crenarchaeota. However, the predicted gene content revealed that several conserved cellular systems, such as cell division, DNA replication, and tRNA maturation, resemble the counterparts in the Euryarchaeota. In light of the known composition of archaeal genomes, the Korarchaeota might have retained a set of cellular features that represents the ancestral archaeal form.
ESTHER : Elkins_2008_Proc.Natl.Acad.Sci.U.S.A_105_8102
PubMedSearch : Elkins_2008_Proc.Natl.Acad.Sci.U.S.A_105_8102
PubMedID: 18535141

Title : Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity - Lapidus_2008_Chem.Biol.Interact_171_236
Author(s) : Lapidus A , Goltsman E , Auger S , Galleron N , Segurens B , Dossat C , Land ML , Broussolle V , Brillard J , Guinebretiere MH , Sanchis V , Nguen-The C , Lereclus D , Richardson P , Wincker P , Weissenbach J , Ehrlich SD , Sorokin A
Ref : Chemico-Biological Interactions , 171 :236 , 2008
Abstract : The Bacillus cereus group represents sporulating soil bacteria containing pathogenic strains which may cause diarrheic or emetic food poisoning outbreaks. Multiple locus sequence typing revealed a presence in natural samples of these bacteria of about 30 clonal complexes. Application of genomic methods to this group was however biased due to the major interest for representatives closely related to Bacillus anthracis. Albeit the most important food-borne pathogens were not yet defined, existing data indicate that they are scattered all over the phylogenetic tree. The preliminary analysis of the sequences of three genomes discussed in this paper narrows down the gaps in our knowledge of the B. cereus group. The strain NVH391-98 is a rare but particularly severe food-borne pathogen. Sequencing revealed that the strain should be a representative of a novel bacterial species, for which the name Bacillus cytotoxis or Bacillus cytotoxicus is proposed. This strain has a reduced genome size compared to other B. cereus group strains. Genome analysis revealed absence of sigma B factor and the presence of genes encoding diarrheic Nhe toxin, not detected earlier. The strain B. cereus F837/76 represents a clonal complex close to that of B. anthracis. Including F837/76, three such B. cereus strains had been sequenced. Alignment of genomes suggests that B. anthracis is their common ancestor. Since such strains often emerge from clinical cases, they merit a special attention. The third strain, KBAB4, is a typical facultative psychrophile generally found in soil. Phylogenic studies show that in nature it is the most active group in terms of gene exchange. Genomic sequence revealed high presence of extra-chromosomal genetic material (about 530kb) that may account for this phenomenon. Genes coding Nhe-like toxin were found on a big plasmid in this strain. This may indicate a potential mechanism of toxicity spread from the psychrophile strain community. The results of this genomic work and ecological compartments of different strains incite to consider a necessity of creating prophylactic vaccines against bacteria closely related to NVH391-98 and F837/76. Presumably developing of such vaccines can be based on the properties of non-pathogenic strains such as KBAB4 or ATCC14579 reported here or earlier. By comparing the protein coding genes of strains being sequenced in this project to others we estimate the shared proteome, or core genome, in the B. cereus group to be 3000+/-200 genes and the total proteome, or pan-genome, to be 20-25,000 genes.
ESTHER : Lapidus_2008_Chem.Biol.Interact_171_236
PubMedSearch : Lapidus_2008_Chem.Biol.Interact_171_236
PubMedID: 17434157
Gene_locus related to this paper: bacwk-A9VF42 , bacan-BA0954 , bacan-BA5009 , bacan-DHBF , bacce-BC0192 , bacce-BC2141 , bacce-BC2171 , bacce-BC2458 , bacce-BC3133 , bacce-BC4862 , bacce-BC5130 , bacce-c2zq05 , bacce-c2zsv2 , bacce-lipP , bacce-PHAC , bacce-q2edm4 , bacce-q72yu1 , baccn-a7gmc0 , baccn-a7gmj8 , baccn-a7gnr6 , baccn-a7gpf9 , baccn-a7gpl4 , baccn-a7gpr3 , baccn-a7gqf9 , baccn-a7gr85 , baccn-a7gsc6 , baccn-a7gse0 , baccn-a7gt87 , baccn-a7gu34 , baccn-a7gu90 , baccn-a7guq6 , baccr-pepx , bacmy-c3aae7 , bactu-c3ice0 , bacwk-a9vg55 , bacwk-a9vgr5 , bacwk-a9vju9 , bacwk-a9vkh7 , bacwk-a9vm76 , bacwk-a9vql0 , bacwk-a9vqm8 , bacwk-a9vtw7 , bacwk-a9vv54 , baccn-a7gp74

Title : Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography - Normand_2007_Genome.Res_17_7
Author(s) : Normand P , Lapierre P , Tisa LS , Gogarten JP , Alloisio N , Bagnarol E , Bassi CA , Berry AM , Bickhart DM , Choisne N , Couloux A , Cournoyer B , Cruveiller S , Daubin V , Demange N , Francino MP , Goltsman E , Huang Y , Kopp OR , Labarre L , Lapidus A , Lavire C , Marechal J , Martinez M , Mastronunzio JE , Mullin BC , Niemann J , Pujic P , Rawnsley T , Rouy Z , Schenowitz C , Sellstedt A , Tavares F , Tomkins JP , Vallenet D , Valverde C , Wall LG , Wang Y , Medigue C , Benson DR
Ref : Genome Res , 17 :7 , 2007
Abstract : Soil bacteria that also form mutualistic symbioses in plants encounter two major levels of selection. One occurs during adaptation to and survival in soil, and the other occurs in concert with host plant speciation and adaptation. Actinobacteria from the genus Frankia are facultative symbionts that form N(2)-fixing root nodules on diverse and globally distributed angiosperms in the "actinorhizal" symbioses. Three closely related clades of Frankia sp. strains are recognized; members of each clade infect a subset of plants from among eight angiosperm families. We sequenced the genomes from three strains; their sizes varied from 5.43 Mbp for a narrow host range strain (Frankia sp. strain HFPCcI3) to 7.50 Mbp for a medium host range strain (Frankia alni strain ACN14a) to 9.04 Mbp for a broad host range strain (Frankia sp. strain EAN1pec.) This size divergence is the largest yet reported for such closely related soil bacteria (97.8%-98.9% identity of 16S rRNA genes). The extent of gene deletion, duplication, and acquisition is in concert with the biogeographic history of the symbioses and host plant speciation. Host plant isolation favored genome contraction, whereas host plant diversification favored genome expansion. The results support the idea that major genome expansions as well as reductions can occur in facultative symbiotic soil bacteria as they respond to new environments in the context of their symbioses.
ESTHER : Normand_2007_Genome.Res_17_7
PubMedSearch : Normand_2007_Genome.Res_17_7
PubMedID: 17151343
Gene_locus related to this paper: frasn-a8leg3 , fraaa-q0rau9 , fraaa-q0rbj9 , fraaa-q0rc03 , fraaa-q0rc89 , fraaa-q0rci1 , fraaa-q0rdx4 , fraaa-q0ref4 , fraaa-q0rel4 , fraaa-q0req5 , fraaa-q0rev2 , fraaa-q0rfl4 , fraaa-q0rfz5 , fraaa-q0rhz6 , fraaa-q0rjm3 , fraaa-q0rjt2 , fraaa-q0rkm8 , fraaa-q0rkv5 , fraaa-q0rl43 , fraaa-q0rlp9 , fraaa-q0rm04 , fraaa-q0rmn2 , fraaa-q0rmz5 , fraaa-q0rqg7 , fraaa-q0rr69 , fraaa-q0rrm7 , fraaa-q0rs07 , fraaa-q0rt07 , fraaa-q0rt55 , fraaa-q0rt70 , fraaa-q0rt91 , fraaa-q0rtc4 , fraaa-q0rte4 , fraaa-q0rtv2 , fraaa-q0rum6 , frasc-q2j5v5 , frasc-q2j8e6 , frasc-q2jct6 , frasn-a8kx42 , frasn-a8kyp2 , frasn-A8L0F8 , frasn-a8l0g7 , frasn-a8l1j7 , frasn-a8l1t9 , frasn-a8l4h8 , frasn-a8l7f8 , frasn-a8l8i4 , frasn-a8l8k8 , frasn-a8l9e9 , frasn-a8l051 , frasn-a8l115 , frasn-a8l161 , frasn-a8l265 , frasn-a8l268 , frasn-a8l720 , frasn-a8l745 , frasn-a8l755 , frasn-a8l875 , frasn-a8lab3 , frasn-a8lag3 , frasn-a8lb99 , frasn-a8lbd8 , frasn-a8lbj7 , frasn-a8lbj8 , frasn-a8lby7 , frasn-a8ldb7 , frasn-a8ldd0 , frasn-a8le91 , frasn-a8leg6 , frasn-a8leq6 , frasn-a8let0 , frasn-a8lf43 , frasn-a8lfg3 , frasn-a8lfl1 , frasn-a8lgw1 , frasn-a8lgy1 , frasc-q2j553 , frasn-a8l2m3 , fraaa-q0rd38 , frasc-q2j6h2 , frasn-a8lfl3 , frasn-a8leg7 , frasn-a8l5b8 , fraaa-q0rgz4 , fraaa-q0rtv3 , frasn-a8le98 , frasn-a8leb4

Title : The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode - Foster_2005_PLoS.Biol_3_e121
Author(s) : Foster J , Ganatra M , Kamal I , Ware J , Makarova K , Ivanova N , Bhattacharyya A , Kapatral V , Kumar S , Posfai J , Vincze T , Ingram J , Moran L , Lapidus A , Omelchenko M , Kyrpides N , Ghedin E , Wang S , Goltsman E , Joukov V , Ostrovskaya O , Tsukerman K , Mazur M , Comb D , Koonin E , Slatko B
Ref : PLoS Biol , 3 :e121 , 2005
Abstract : Complete genome DNA sequence and analysis is presented for Wolbachia, the obligate alpha-proteobacterial endosymbiont required for fertility and survival of the human filarial parasitic nematode Brugia malayi. Although, quantitatively, the genome is even more degraded than those of closely related Rickettsia species, Wolbachia has retained more intact metabolic pathways. The ability to provide riboflavin, flavin adenine dinucleotide, heme, and nucleotides is likely to be Wolbachia's principal contribution to the mutualistic relationship, whereas the host nematode likely supplies amino acids required for Wolbachia growth. Genome comparison of the Wolbachia endosymbiont of B. malayi (wBm) with the Wolbachia endosymbiont of Drosophila melanogaster (wMel) shows that they share similar metabolic trends, although their genomes show a high degree of genome shuffling. In contrast to wMel, wBm contains no prophage and has a reduced level of repeated DNA. Both Wolbachia have lost a considerable number of membrane biogenesis genes that apparently make them unable to synthesize lipid A, the usual component of proteobacterial membranes. However, differences in their peptidoglycan structures may reflect the mutualistic lifestyle of wBm in contrast to the parasitic lifestyle of wMel. The smaller genome size of wBm, relative to wMel, may reflect the loss of genes required for infecting host cells and avoiding host defense systems. Analysis of this first sequenced endosymbiont genome from a filarial nematode provides insight into endosymbiont evolution and additionally provides new potential targets for elimination of cutaneous and lymphatic human filarial disease.
ESTHER : Foster_2005_PLoS.Biol_3_e121
PubMedSearch : Foster_2005_PLoS.Biol_3_e121
PubMedID: 15780005
Gene_locus related to this paper: woltr-q5gs90 , woltr-q5gtr0

Title : Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000 - Feil_2005_Proc.Natl.Acad.Sci.U.S.A_102_11064
Author(s) : Feil H , Feil WS , Chain P , Larimer F , DiBartolo G , Copeland A , Lykidis A , Trong S , Nolan M , Goltsman E , Thiel J , Malfatti S , Loper JE , Lapidus A , Detter JC , Land M , Richardson PM , Kyrpides NC , Ivanova N , Lindow SE
Ref : Proc Natl Acad Sci U S A , 102 :11064 , 2005
Abstract : The complete genomic sequence of Pseudomonas syringae pv. syringae B728a (Pss B728a) has been determined and is compared with that of P. syringae pv. tomato DC3000 (Pst DC3000). The two pathovars of this economically important species of plant pathogenic bacteria differ in host range and other interactions with plants, with Pss having a more pronounced epiphytic stage of growth and higher abiotic stress tolerance and Pst DC3000 having a more pronounced apoplastic growth habitat. The Pss B728a genome (6.1 Mb) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared with Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. Over 375 repetitive extragenic palindromic sequences unique to Pss B728a when compared with Pst DC3000 are widely distributed throughout the chromosome except in 14 genomic islands, which generally had lower GC content than the genome as a whole. Content of the genomic islands varies, with one containing a prophage and another the plasmid pKLC102 of Pseudomonas aeruginosa PAO1. Among the 976 genes of Pss B728a with no counterpart in Pst DC3000 are those encoding for syringopeptin, syringomycin, indole acetic acid biosynthesis, arginine degradation, and production of ice nuclei. The genomic comparison suggests that several unique genes for Pss B728a such as ectoine synthase, DNA repair, and antibiotic production may contribute to the epiphytic fitness and stress tolerance of this organism.
ESTHER : Feil_2005_Proc.Natl.Acad.Sci.U.S.A_102_11064
PubMedSearch : Feil_2005_Proc.Natl.Acad.Sci.U.S.A_102_11064
PubMedID: 16043691
Gene_locus related to this paper: psesm-METX , psesm-q87y20 , psesm-q889k3 , psesy-PIP , psesy-PSPTO2134 , psesy-PSPTO3135 , psesy-SYLD , psesy-SYPC , psesy-SYRE , pseu2-q4zlt8 , pseu2-q4zm40 , pseu2-q4zmb0 , pseu2-q4zmc0 , pseu2-q4zmh2 , pseu2-q4zmw4 , pseu2-q4zn59 , pseu2-q4znb2 , pseu2-q4znm5 , pseu2-q4zpb0 , pseu2-q4zph7 , pseu2-q4zpw6 , pseu2-q4zq18 , pseu2-q4zq29 , pseu2-q4zqk3 , pseu2-q4zr46 , pseu2-q4zrk3 , pseu2-q4zrq0 , pseu2-q4zrr1 , pseu2-q4zrt9 , pseu2-q4zs33 , pseu2-q4zs84 , pseu2-q4zs88 , pseu2-q4zsh3 , pseu2-q4zt45 , pseu2-q4zt74 , pseu2-q4zta6 , pseu2-q4zte9 , pseu2-q4zts5 , pseu2-q4zun8 , pseu2-q4zuv2 , pseu2-q4zuw1 , pseu2-q4zv19 , pseu2-q4zv57 , pseu2-q4zvi1 , pseu2-q4zvi2 , pseu2-q4zw50 , pseu2-q4zwq3 , pseu2-q4zwv7 , pseu2-q4zxj5 , pseu2-q4zxs0 , pseu2-q4zz02 , pseu2-q4zzm1 , pseu2-q4zzm4 , pseu2-q500r7 , pseu2-q4zqx8

Title : Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus - Bolotin_2004_Nat.Biotechnol_22_1554
Author(s) : Bolotin A , Quinquis B , Renault P , Sorokin A , Ehrlich SD , Kulakauskas S , Lapidus A , Goltsman E , Mazur M , Pusch GD , Fonstein M , Overbeek R , Kyprides N , Purnelle B , Prozzi D , Ngui K , Masuy D , Hancy F , Burteau S , Boutry M , Delcour J , Goffeau A , Hols P
Ref : Nat Biotechnol , 22 :1554 , 2004
Abstract : The lactic acid bacterium Streptococcus thermophilus is widely used for the manufacture of yogurt and cheese. This dairy species of major economic importance is phylogenetically close to pathogenic streptococci, raising the possibility that it has a potential for virulence. Here we report the genome sequences of two yogurt strains of S. thermophilus. We found a striking level of gene decay (10% pseudogenes) in both microorganisms. Many genes involved in carbon utilization are nonfunctional, in line with the paucity of carbon sources in milk. Notably, most streptococcal virulence-related genes that are not involved in basic cellular processes are either inactivated or absent in the dairy streptococcus. Adaptation to the constant milk environment appears to have resulted in the stabilization of the genome structure. We conclude that S. thermophilus has evolved mainly through loss-of-function events that remarkably mirror the environment of the dairy niche resulting in a severely diminished pathogenic potential.
ESTHER : Bolotin_2004_Nat.Biotechnol_22_1554
PubMedSearch : Bolotin_2004_Nat.Biotechnol_22_1554
PubMedID: 15543133
Gene_locus related to this paper: strt1-q5lz16 , strt1-q5lza1 , strt2-q5m420 , strtr-pepx

Title : Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586 - Kapatral_2002_J.Bacteriol_184_2005
Author(s) : Kapatral V , Anderson I , Ivanova N , Reznik G , Los T , Lykidis A , Bhattacharyya A , Bartman A , Gardner W , Grechkin G , Zhu L , Vasieva O , Chu L , Kogan Y , Chaga O , Goltsman E , Bernal A , Larsen N , D'Souza M , Walunas T , Pusch G , Haselkorn R , Fonstein M , Kyrpides N , Overbeek R
Ref : Journal of Bacteriology , 184 :2005 , 2002
Abstract : We present a complete DNA sequence and metabolic analysis of the dominant oral bacterium Fusobacterium nucleatum. Although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens Porphyromonas gingivalis and Bacteroides forsythus. The F. nucleatum strain ATCC 25586 genome was assembled from shotgun sequences and analyzed using the ERGO bioinformatics suite (http://www.integratedgenomics.com). The genome contains 2.17 Mb encoding 2,067 open reading frames, organized on a single circular chromosome with 27% GC content. Despite its taxonomic position among the gram-negative bacteria, several features of its core metabolism are similar to that of gram-positive Clostridium spp., Enterococcus spp., and Lactococcus spp. The genome analysis has revealed several key aspects of the pathways of organic acid, amino acid, carbohydrate, and lipid metabolism. Nine very-high-molecular-weight outer membrane proteins are predicted from the sequence, none of which has been reported in the literature. More than 137 transporters for the uptake of a variety of substrates such as peptides, sugars, metal ions, and cofactors have been identified. Biosynthetic pathways exist for only three amino acids: glutamate, aspartate, and asparagine. The remaining amino acids are imported as such or as di- or oligopeptides that are subsequently degraded in the cytoplasm. A principal source of energy appears to be the fermentation of glutamate to butyrate. Additionally, desulfuration of cysteine and methionine yields ammonia, H(2)S, methyl mercaptan, and butyrate, which are capable of arresting fibroblast growth, thus preventing wound healing and aiding penetration of the gingival epithelium. The metabolic capabilities of F. nucleatum revealed by its genome are therefore consistent with its specialized niche in the mouth.
ESTHER : Kapatral_2002_J.Bacteriol_184_2005
PubMedSearch : Kapatral_2002_J.Bacteriol_184_2005
PubMedID: 11889109
Gene_locus related to this paper: fusnn-FN0616 , fusnn-FN0752 , fusnu-FN0280 , fusnu-FN0484 , fusnu-FN0852 , fusnu-FN1062 , fusnu-FN1128 , fusnu-FN1345 , fusnv-q7p2e5 , fusnv-q7p338

Title : The genome sequence of the facultative intracellular pathogen Brucella melitensis - DelVecchio_2002_Proc.Natl.Acad.Sci.U.S.A_99_443
Author(s) : DelVecchio VG , Kapatral V , Redkar RJ , Patra G , Mujer C , Los T , Ivanova N , Anderson I , Bhattacharyya A , Lykidis A , Reznik G , Jablonski L , Larsen N , D'Souza M , Bernal A , Mazur M , Goltsman E , Selkov E , Elzer PH , Hagius S , O'Callaghan D , Letesson JJ , Haselkorn R , Kyrpides N , Overbeek R
Ref : Proc Natl Acad Sci U S A , 99 :443 , 2002
Abstract : Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and Malta fever in humans. The genome of B. melitensis strain 16M was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 ORFs. By using the bioinformatics suite ERGO, 2,487 (78%) ORFs were assigned functions. The origins of replication of the two chromosomes are similar to those of other alpha-proteobacteria. Housekeeping genes, including those involved in DNA replication, transcription, translation, core metabolism, and cell wall biosynthesis, are distributed on both chromosomes. Type I, II, and III secretion systems are absent, but genes encoding sec-dependent, sec-independent, and flagella-specific type III, type IV, and type V secretion systems as well as adhesins, invasins, and hemolysins were identified. Several features of the B. melitensis genome are similar to those of the symbiotic Sinorhizobium meliloti.
ESTHER : DelVecchio_2002_Proc.Natl.Acad.Sci.U.S.A_99_443
PubMedSearch : DelVecchio_2002_Proc.Natl.Acad.Sci.U.S.A_99_443
PubMedID: 11756688
Gene_locus related to this paper: brume-BMEI0552 , brume-BMEI0733 , brume-BMEI1044 , brume-BMEI1119 , brume-BMEI1365 , brume-BMEI1594 , brume-BMEI1608 , brume-BMEI1822 , brume-BMEI1884 , brume-BMEI1951 , brume-BMEI2011 , brume-BMEII0047 , brume-BMEII0306 , brume-BMEII0681 , brume-BMEII0989 , brume-dhE2 , brume-PCAD , brusu-BR0288 , brusu-BR1291 , brusu-BR1327