D74E_human-ACHE

General

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Peripheral Anionic Site Peripheral Anionic Site\/signal transduction\/allosteric modulation. Affects conformation of active site and PAS Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 || Signal transduction Affects conformation of active site and PAS Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 || VX stereospecificity no effect in stereoselectivity Ordentlich_2005_Chem.Biol.Interact_157-158_191 || oxime interaction reactivation of tabun-inhibited human AChE. Unable to facilitate HI-6-mediated reactivation of tabun-hAChE Artursson_2009_Toxicology_265_108

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Peripheral Anionic Site || Substrate inhibition || Signal transduction || Allosteric modulation || Oxime interaction

Torpedo_number : 72

Kinetic Parameter : BW284C51_D74E_human-ACHE, Decamethonium_D74E_human-ACHE, Propidium_D74E_human-ACHE, Edrophonium_D74E_human-ACHE, Acetylthiocholine_D74E_human-ACHE

News : No news

Comment :
p.D74E Asp74Glu (p.D105E Asp105Glu in primary sequence with 31 amino-acids signal peptide) Peripheral Anionic Site\/signal transduction\/allosteric modulation. Affects conformation of active site and PAS\; no effect in VX stereoselectivity\; Unable to facilitate HI-6-mediated reactivation of tabun-hAChE

References (4)

Title : Reactivation of tabun-hAChE investigated by structurally analogous oximes and mutagenesis - Artursson_2009_Toxicology_265_108
Author(s) : Artursson E , Akfur C , Hornberg A , Worek F , Ekstrom F
Ref : Toxicology , 265 :108 , 2009
Abstract :
PubMedSearch : Artursson_2009_Toxicology_265_108
PubMedID: 19761810

Title : The role of AChE active site gorge in determining stereoselectivity of charged and noncharged VX enantiomers - Ordentlich_2005_Chem.Biol.Interact_157-158_191
Author(s) : Ordentlich A , Barak D , Sod-Moriah G , Kaplan D , Mizrahi D , Segall Y , Kronman C , Karton Y , Lazar A , Marcus D , Velan B , Shafferman A
Ref : Chemico-Biological Interactions , 157-158 :191 , 2005
Abstract :
PubMedSearch : Ordentlich_2005_Chem.Biol.Interact_157-158_191
PubMedID: 16289014

Title : Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center - Shafferman_1992_EMBO.J_11_3561
Author(s) : Shafferman A , Velan B , Ordentlich A , Kronman C , Grosfeld H , Leitner M , Flashner Y , Cohen S , Barak D , Ariel N
Ref : EMBO Journal , 11 :3561 , 1992
Abstract :
PubMedSearch : Shafferman_1992_EMBO.J_11_3561
PubMedID: 1396557

Title : Acetylcholinesterase Catalysis - Protein Engineering Studies -
Author(s) : Shafferman A , Velan B
Ref : In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases , (Shafferman, A. and Velan, B., Eds) Plenum Press, New York :165 , 1992
PubMedID: