L286H_human-BCHE

General

Gene Locus : human-BCHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Search for phosphotriesterase activity Phosphotriesterase activity Identical to WT Lockridge_1997_Biochemistry_36_786 || Search for phosphotriesterase activity Phosphotriesterase activity OP sensitive Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Search for phosphotriesterase activity

Torpedo_number : 288

Kinetic Parameter : No kinetic parameter

News : No news

Comment :
p.L286H Leu286His (p.L314H Leu314His in primary sequence with 28 amino-acids signal peptide) Phosphotriesterase activity Identical to WT. Tentative to place an Histidine in the active site at other place than the G117H mutation. Though the L286H mutant reactivated 400-fold more slowly than G117H, it was still 250-fold faster than wild-type BChE. Adjusting the position of histidine 286 by adding glycine residues to the sequence, e.g., L286GH, L286HG, and L286GHG, did not improve the reactivation rate

References (2)

Title : Mutants of human butyrylcholinesterase with organophosphate hydrolase activity\; evidence that His117 is a general base catalyst for hydrolysis of echothiophate - Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1
Author(s) : Schopfer LM , Boeck AT , Broomfield CA , Lockridge O
Ref : Journal of Medicinal Chemistryical Biology Radiol Def , 2 :1 , 2004
Abstract :
PubMedSearch : Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1
PubMedID:

Title : A single amino acid substitution, Gly117His, confers phosphotriesterase (organophosphorus acid anhydride hydrolase) activity on human butyrylcholinesterase - Lockridge_1997_Biochemistry_36_786
Author(s) : Lockridge O , Blong RM , Masson P , Froment MT , Millard CB , Broomfield CA
Ref : Biochemistry , 36 :786 , 1997
Abstract :
PubMedSearch : Lockridge_1997_Biochemistry_36_786
PubMedID: 9020776